Mutagenetix > Incidental Mutation

Incidental Mutation 'R8978:Or7g21'

683572

Institutional Source

Beutler Lab

Gene Symbol

Or7g21

Ensembl Gene

ENSMUSG00000059303

Gene Name

olfactory receptor family 7 subfamily G member 21

Synonyms

GA_x6K02T2PVTD-12857805-12858749, MOR152-3, Olfr836

MMRRC Submission

068811-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.150) question?

Stock #

R8978 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19032253-19033207 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 19032582 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 107 (C107*)

Ref Sequence

ENSEMBL: ENSMUSP00000148738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074986] [ENSMUST00000212730] [ENSMUST00000215981]

AlphaFold

Q8VFJ3

Predicted Effect

probably null
Transcript: ENSMUST00000074986
AA Change: C110*

SMART Domains

Protein: ENSMUSP00000074514
Gene: ENSMUSG00000059303
AA Change: C110*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	1.2e-50	PFAM
Pfam:7TM_GPCR_Srsx	38	210	9.6e-6	PFAM
Pfam:7tm_1	44	293	2.4e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000212730
AA Change: C107*

Predicted Effect

probably null
Transcript: ENSMUST00000215981
AA Change: C107*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.8%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg4	A	G	9: 44,192,395 (GRCm39)	F224L	probably benign	Het
Adam39	A	G	8: 41,278,707 (GRCm39)	D366G	probably damaging	Het
Adamts14	T	A	10: 61,038,795 (GRCm39)	E902V	probably damaging	Het
Adamts6	T	C	13: 104,512,247 (GRCm39)	C490R	probably damaging	Het
Btbd3	T	C	2: 138,126,055 (GRCm39)	V413A	possibly damaging	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,128,173 (GRCm39)		probably benign	Het
Cdadc1	G	T	14: 59,813,197 (GRCm39)	N403K	probably damaging	Het
Cdc27	C	T	11: 104,399,211 (GRCm39)	V734I	possibly damaging	Het
Celf3	A	G	3: 94,392,667 (GRCm39)	D122G	probably benign	Het
Chgb	T	C	2: 132,634,498 (GRCm39)	S147P	probably benign	Het
Col9a1	G	A	1: 24,278,396 (GRCm39)	G808D	probably damaging	Het
Cpne7	A	G	8: 123,861,177 (GRCm39)		probably null	Het
Crhr1	T	C	11: 104,064,480 (GRCm39)	F330S	possibly damaging	Het
Csmd1	A	C	8: 15,971,056 (GRCm39)	N3086K	probably benign	Het
Ctnnb1	A	G	9: 120,786,650 (GRCm39)	D624G	probably damaging	Het
Dcaf8	G	A	1: 172,022,124 (GRCm39)	R554H	probably benign	Het
Dmbt1	T	C	7: 130,639,611 (GRCm39)	Y50H	possibly damaging	Het
Dmxl1	T	C	18: 50,055,679 (GRCm39)	L2573P	probably benign	Het
Ebf2	G	A	14: 67,661,548 (GRCm39)	G559S	probably benign	Het
Fancd2	A	G	6: 113,562,507 (GRCm39)		probably benign	Het
Fkbp10	A	T	11: 100,313,936 (GRCm39)	I427F	probably benign	Het
Gipc1	T	C	8: 84,389,046 (GRCm39)		probably null	Het
Gm1110	A	T	9: 26,807,095 (GRCm39)		probably benign	Het
Gsdmc3	A	G	15: 63,731,455 (GRCm39)	L359P	probably damaging	Het
Hspg2	A	G	4: 137,291,341 (GRCm39)	D3897G	probably benign	Het
Ift122	T	A	6: 115,902,769 (GRCm39)	M1117K	possibly damaging	Het
Itgbl1	A	T	14: 124,209,617 (GRCm39)	D456V	probably damaging	Het
Itprid2	T	A	2: 79,491,257 (GRCm39)	L1014Q	probably damaging	Het
Kctd1	G	T	18: 15,119,491 (GRCm39)	L675I		Het
Kyat3	C	T	3: 142,443,596 (GRCm39)	T403M	probably benign	Het
Ltbp2	T	C	12: 84,834,164 (GRCm39)	T1442A	probably benign	Het
Mier2	A	C	10: 79,376,790 (GRCm39)	S45R	unknown	Het
Mon2	T	A	10: 122,871,469 (GRCm39)	K383*	probably null	Het
Mroh4	G	T	15: 74,499,473 (GRCm39)	T136K	probably benign	Het
Mtif3	A	T	5: 146,895,846 (GRCm39)	S80R	probably benign	Het
Myh2	A	C	11: 67,068,188 (GRCm39)	E272A	probably damaging	Het
Myh2	A	G	11: 67,080,323 (GRCm39)	Q1179R	probably damaging	Het
Nfatc3	G	A	8: 106,835,402 (GRCm39)	C916Y	probably benign	Het
Or13a17	A	T	7: 140,271,642 (GRCm39)	T275S	probably benign	Het
Or2w2	T	A	13: 21,758,279 (GRCm39)	T116S	probably benign	Het
Orc2	T	C	1: 58,511,499 (GRCm39)	D370G	possibly damaging	Het
Pate7	A	T	9: 35,688,069 (GRCm39)		probably benign	Het
Pcdhga6	T	A	18: 37,840,716 (GRCm39)	N145K	probably benign	Het
Pih1d2	A	G	9: 50,536,232 (GRCm39)	M296V	probably benign	Het
Prickle4	A	T	17: 47,999,772 (GRCm39)		probably benign	Het
Rabl6	G	A	2: 25,477,541 (GRCm39)	P303L	probably damaging	Het
Rara	T	G	11: 98,862,595 (GRCm39)	I332S	probably damaging	Het
Reln	A	G	5: 22,090,512 (GRCm39)	F3449L	possibly damaging	Het
Rhoq	T	C	17: 87,271,767 (GRCm39)	L61P		Het
Rigi	A	G	4: 40,239,650 (GRCm39)	I16T	probably damaging	Het
Slc6a15	G	A	10: 103,230,953 (GRCm39)	W226*	probably null	Het
Spef2	A	T	15: 9,725,263 (GRCm39)	S165T	possibly damaging	Het
Stab2	T	C	10: 86,785,782 (GRCm39)	N620S	possibly damaging	Het
Suds3	A	G	5: 117,232,973 (GRCm39)		probably null	Het
Sult1b1	A	G	5: 87,682,900 (GRCm39)	I15T	possibly damaging	Het
Synm	C	A	7: 67,384,672 (GRCm39)	V997L	probably damaging	Het
Tgfbi	C	A	13: 56,778,391 (GRCm39)	D387E	probably benign	Het
Thrb	T	C	14: 17,981,886 (GRCm38)	C4R	possibly damaging	Het
Tk2	A	G	8: 104,957,809 (GRCm39)	V179A	possibly damaging	Het
Trim25	T	C	11: 88,907,027 (GRCm39)	V462A	probably benign	Het
Tub	T	C	7: 108,629,393 (GRCm39)	Y483H	probably damaging	Het
Vav1	A	G	17: 57,603,710 (GRCm39)	T131A	probably benign	Het
Vav1	A	G	17: 57,631,650 (GRCm39)	T781A	probably benign	Het
Zbtb34	A	G	2: 33,301,048 (GRCm39)	S498P	possibly damaging	Het
Zfp568	T	A	7: 29,716,683 (GRCm39)	H194Q	probably benign	Het
Zfp683	A	T	4: 133,781,239 (GRCm39)	Q18L	probably benign	Het

Other mutations in Or7g21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00907:Or7g21	APN	9	19,032,528 (GRCm39)	missense	possibly damaging	0.88
IGL01316:Or7g21	APN	9	19,032,718 (GRCm39)	missense	probably benign	0.10
IGL01412:Or7g21	APN	9	19,032,895 (GRCm39)	missense	probably benign	0.00
IGL01815:Or7g21	APN	9	19,032,622 (GRCm39)	missense	probably damaging	1.00
IGL02003:Or7g21	APN	9	19,032,361 (GRCm39)	missense	probably benign	0.06
IGL02313:Or7g21	APN	9	19,032,671 (GRCm39)	missense	probably damaging	0.99
IGL03185:Or7g21	APN	9	19,033,034 (GRCm39)	missense	probably damaging	1.00
IGL03186:Or7g21	APN	9	19,033,200 (GRCm39)	missense	probably benign	0.16
R1337:Or7g21	UTSW	9	19,033,099 (GRCm39)	missense	probably benign
R2267:Or7g21	UTSW	9	19,032,737 (GRCm39)	missense	probably benign	0.22
R3969:Or7g21	UTSW	9	19,032,956 (GRCm39)	missense	probably benign	0.00
R4695:Or7g21	UTSW	9	19,032,306 (GRCm39)	missense	probably null	0.04
R4976:Or7g21	UTSW	9	19,032,797 (GRCm39)	missense	probably damaging	1.00
R5176:Or7g21	UTSW	9	19,032,656 (GRCm39)	missense	probably damaging	1.00
R5379:Or7g21	UTSW	9	19,032,373 (GRCm39)	missense	probably damaging	1.00
R5638:Or7g21	UTSW	9	19,032,676 (GRCm39)	missense	probably benign	0.00
R6084:Or7g21	UTSW	9	19,032,623 (GRCm39)	missense	probably damaging	1.00
R6236:Or7g21	UTSW	9	19,032,409 (GRCm39)	missense	possibly damaging	0.92
R6329:Or7g21	UTSW	9	19,032,253 (GRCm39)	start codon destroyed	probably benign	0.08
R7151:Or7g21	UTSW	9	19,033,037 (GRCm39)	missense	possibly damaging	0.91
R7326:Or7g21	UTSW	9	19,032,965 (GRCm39)	missense	probably benign	0.42
R8460:Or7g21	UTSW	9	19,032,988 (GRCm39)	missense	probably damaging	1.00
R8767:Or7g21	UTSW	9	19,032,922 (GRCm39)	missense	possibly damaging	0.95
R9099:Or7g21	UTSW	9	19,032,890 (GRCm39)	missense	probably benign	0.35
R9220:Or7g21	UTSW	9	19,033,193 (GRCm39)	missense	possibly damaging	0.82
R9236:Or7g21	UTSW	9	19,033,206 (GRCm39)	makesense	probably null
R9265:Or7g21	UTSW	9	19,032,984 (GRCm39)	nonsense	probably null
R9530:Or7g21	UTSW	9	19,033,051 (GRCm39)	missense	probably benign	0.24
R9612:Or7g21	UTSW	9	19,032,760 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- ACAGAGCTTCAGCCCTTTATC -3'
(R):5'- GCACCATCAGACTATGCAGC -3'

Sequencing Primer
(F):5'- ATATTGGCCATCATTTGTGACTC -3'
(R):5'- GACTATGCAGCAGACCATTTAC -3'

Posted On

2021-10-11