Mutagenetix > Incidental Mutation

Incidental Mutation 'R0748:Lcn4'

70211

Institutional Source

Beutler Lab

Gene Symbol

Lcn4

Ensembl Gene

ENSMUSG00000026919

Gene Name

lipocalin 4

Synonyms

A630045M08Rik, Vnsp2

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R0748 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

26557686-26561294 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 26558359 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 175 (I175M)

Ref Sequence

ENSEMBL: ENSMUSP00000028283 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028283]

AlphaFold

Q62472

Predicted Effect

probably damaging
Transcript: ENSMUST00000028283
AA Change: I175M

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000028283
Gene: ENSMUSG00000026919
AA Change: I175M

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:Lipocalin	33	170	1.3e-21	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.7%
20x: 92.4%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf3	A	G	5: 30,401,874 (GRCm39)	V718A	probably damaging	Het
Amigo1	A	G	3: 108,095,946 (GRCm39)	S482G	probably damaging	Het
F2rl3	A	G	8: 73,489,379 (GRCm39)	Q202R	probably benign	Het
Faap100	A	G	11: 120,262,997 (GRCm39)	V787A	probably damaging	Het
Fat4	A	T	3: 38,941,977 (GRCm39)	Q290L	possibly damaging	Het
Flnc	A	G	6: 29,446,343 (GRCm39)	E920G	probably damaging	Het
Gm9881	T	C	16: 90,967,313 (GRCm39)	N137S	unknown	Het
Hps4	A	G	5: 112,522,780 (GRCm39)	E546G	probably damaging	Het
Htr2b	A	G	1: 86,038,528 (GRCm39)	I26T	probably benign	Het
Insr	A	C	8: 3,308,841 (GRCm39)	M65R	probably damaging	Het
Kif20a	T	C	18: 34,761,241 (GRCm39)		probably benign	Het
L3mbtl1	A	T	2: 162,813,083 (GRCm39)		probably benign	Het
L3mbtl1	A	C	2: 162,813,084 (GRCm39)		probably null	Het
Malt1	T	A	18: 65,608,331 (GRCm39)		probably null	Het
Nufip1	T	C	14: 76,348,508 (GRCm39)	S46P	probably damaging	Het
Nup93	T	A	8: 95,034,571 (GRCm39)	Y629N	probably damaging	Het
Or10a49	C	A	7: 108,468,357 (GRCm39)	M1I	probably null	Het
Pcsk6	T	C	7: 65,688,716 (GRCm39)		probably benign	Het
Rdx	G	C	9: 51,976,160 (GRCm39)	V33L	possibly damaging	Het
Rnf213	T	C	11: 119,364,306 (GRCm39)	L4535P	probably damaging	Het
Rorb	T	A	19: 18,955,164 (GRCm39)	T66S	probably damaging	Het
S100a9	T	C	3: 90,600,198 (GRCm39)	D66G	possibly damaging	Het
Sacs	T	C	14: 61,446,714 (GRCm39)	I2920T	probably damaging	Het
Safb2	T	C	17: 56,882,580 (GRCm39)	N351S	probably benign	Het
Ugt1a10	C	T	1: 88,142,845 (GRCm39)	P113L	probably damaging	Het
Unc13b	G	A	4: 43,241,164 (GRCm39)		probably benign	Het
Vars1	A	G	17: 35,216,988 (GRCm39)	S489P	probably damaging	Het
Wars2	A	G	3: 99,123,888 (GRCm39)	K250E	probably damaging	Het
Zfp292	A	G	4: 34,816,424 (GRCm39)		probably benign	Het

Other mutations in Lcn4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0421:Lcn4	UTSW	2	26,558,661 (GRCm39)	missense	possibly damaging	0.73
R1466:Lcn4	UTSW	2	26,558,588 (GRCm39)	missense	probably damaging	1.00
R1466:Lcn4	UTSW	2	26,558,588 (GRCm39)	missense	probably damaging	1.00
R1584:Lcn4	UTSW	2	26,558,588 (GRCm39)	missense	probably damaging	1.00
R1911:Lcn4	UTSW	2	26,560,607 (GRCm39)	splice site	probably benign
R2008:Lcn4	UTSW	2	26,561,228 (GRCm39)	missense	possibly damaging	0.85
R6953:Lcn4	UTSW	2	26,559,367 (GRCm39)	missense	probably benign
R7369:Lcn4	UTSW	2	26,557,906 (GRCm39)	missense	probably benign	0.01
R8262:Lcn4	UTSW	2	26,558,375 (GRCm39)	missense	probably benign	0.00
R8968:Lcn4	UTSW	2	26,558,360 (GRCm39)	missense	possibly damaging	0.79
R9513:Lcn4	UTSW	2	26,560,613 (GRCm39)	critical splice donor site	probably null
R9622:Lcn4	UTSW	2	26,561,228 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCTGAGAATACATGGGCCACAGAAG -3'
(R):5'- GCCTCTGTTCACAGGAAAGTATCCG -3'

Sequencing Primer
(F):5'- AGTATCCAAGAATTCCACTTGAGGAC -3'
(R):5'- AAGAGGATGCTCTGTTTAACTTCTCC -3'

Posted On

2013-09-30