Incidental Mutation 'R9348:Crlf1'
ID 707924
Institutional Source Beutler Lab
Gene Symbol Crlf1
Ensembl Gene ENSMUSG00000007888
Gene Name cytokine receptor-like factor 1
Synonyms cytokine receptor like molecule 3, CRLM3, CLF-1
MMRRC Submission
Accession Numbers
Essential gene? Probably essential (E-score: 0.791) question?
Stock # R9348 (G1)
Quality Score 225.009
Status Not validated
Chromosome 8
Chromosomal Location 70945808-70956731 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 70951316 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Lysine at position 65 (I65K)
Ref Sequence ENSEMBL: ENSMUSP00000008032 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000008032] [ENSMUST00000132648]
AlphaFold Q9JM58
Predicted Effect probably benign
Transcript: ENSMUST00000008032
AA Change: I65K

PolyPhen 2 Score 0.209 (Sensitivity: 0.92; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000008032
Gene: ENSMUSG00000007888
AA Change: I65K

DomainStartEndE-ValueType
signal peptide 1 40 N/A INTRINSIC
Pfam:Lep_receptor_Ig 41 127 5.7e-8 PFAM
FN3 138 223 2.11e0 SMART
FN3 238 323 1.5e-5 SMART
low complexity region 345 361 N/A INTRINSIC
low complexity region 415 425 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000127983
SMART Domains Protein: ENSMUSP00000115614
Gene: ENSMUSG00000007888

DomainStartEndE-ValueType
Blast:FN3 2 28 2e-12 BLAST
SCOP:d1eerb2 2 46 1e-8 SMART
low complexity region 50 66 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000132648
SMART Domains Protein: ENSMUSP00000119545
Gene: ENSMUSG00000007888

DomainStartEndE-ValueType
FN3 16 101 2.11e0 SMART
low complexity region 104 122 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.5%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a member of the cytokine type I receptor family. The encoded protein functions as a cytokine receptor subunit and may be involved in immune system regulation and fetal development. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for a targeted mutation fail to suckle effectively and do not survive beyond 24 hrs after birth. Newborns exhibit reduced numbers of hematopoietic progenitor cells as well as a significant reduction in the number of motoneurons in the lumbar spinal cord and facial nucleus. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 50 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam25 C T 8: 41,208,953 (GRCm39) P740S probably benign Het
Arfgef1 G T 1: 10,283,419 (GRCm39) H163Q probably benign Het
Arih2 C G 9: 108,488,938 (GRCm39) R260P probably damaging Het
Atp6v1h T A 1: 5,187,699 (GRCm39) D222E probably damaging Het
Auts2 T C 5: 131,490,155 (GRCm39) T165A Het
BC034090 A G 1: 155,099,049 (GRCm39) S579P probably benign Het
Bpifb9b T A 2: 154,160,766 (GRCm39) S546T probably benign Het
C4b C T 17: 34,952,159 (GRCm39) V1229I probably benign Het
Cadps2 T C 6: 23,344,262 (GRCm39) D895G probably benign Het
Cby2 A G 14: 75,820,838 (GRCm39) S296P probably damaging Het
Cep112 T C 11: 108,328,076 (GRCm39) S51P probably damaging Het
Cep164 T C 9: 45,717,708 (GRCm39) H545R unknown Het
Creb3l1 C T 2: 91,822,231 (GRCm39) probably null Het
Crybg3 T C 16: 59,421,256 (GRCm39) M1V probably null Het
Cyb5d1 T A 11: 69,285,830 (GRCm39) I69L probably damaging Het
D430041D05Rik T C 2: 104,088,337 (GRCm39) D213G probably benign Het
Dennd5a C G 7: 109,498,930 (GRCm39) probably null Het
Dennd5a T A 7: 109,498,942 (GRCm39) M998L probably benign Het
E2f1 T A 2: 154,402,755 (GRCm39) E382D probably benign Het
Enpep T A 3: 129,102,772 (GRCm39) T395S probably benign Het
Gm20939 A G 17: 95,182,977 (GRCm39) E71G probably damaging Het
Hs1bp3 T A 12: 8,386,273 (GRCm39) V225E probably benign Het
Icam5 T C 9: 20,943,427 (GRCm39) M1T probably null Het
Ifi205 T C 1: 173,844,997 (GRCm39) I262V probably benign Het
Iqce T C 5: 140,677,380 (GRCm39) Y147C probably damaging Het
Kcnh2 C T 5: 24,538,003 (GRCm39) G120E probably damaging Het
Kif3c A G 12: 3,417,505 (GRCm39) T509A probably benign Het
Napepld A T 5: 21,875,490 (GRCm39) N351K probably benign Het
Ndufaf3 C A 9: 108,443,357 (GRCm39) probably null Het
Nlrp9b C T 7: 19,757,336 (GRCm39) T191I probably damaging Het
Oog2 T C 4: 143,921,789 (GRCm39) L233P probably damaging Het
Or1a1b T A 11: 74,097,289 (GRCm39) Y251F probably damaging Het
Or2g1 T C 17: 38,106,992 (GRCm39) I219T probably damaging Het
Or2n1d A T 17: 38,646,320 (GRCm39) T91S possibly damaging Het
Or2w1 T A 13: 21,317,131 (GRCm39) F62Y possibly damaging Het
Preb A G 5: 31,112,995 (GRCm39) F416L probably benign Het
Prickle2 C T 6: 92,397,243 (GRCm39) V217I probably benign Het
Prss45 T A 9: 110,668,278 (GRCm39) I157N probably damaging Het
Ranbp17 T C 11: 33,429,232 (GRCm39) T338A probably benign Het
Snd1 T C 6: 28,745,206 (GRCm39) F517S probably damaging Het
Srpk2 A T 5: 23,719,671 (GRCm39) F578I probably damaging Het
Stx2 A T 5: 129,076,601 (GRCm39) D27E probably benign Het
Tgfbrap1 A G 1: 43,093,695 (GRCm39) I599T probably benign Het
Trim25 G T 11: 88,900,167 (GRCm39) E305* probably null Het
Ttll7 T A 3: 146,673,768 (GRCm39) I821N probably benign Het
Wdfy3 A G 5: 102,089,358 (GRCm39) L612P probably damaging Het
Zcchc9 T A 13: 91,954,186 (GRCm39) D23V Het
Zfp141 C A 7: 42,124,814 (GRCm39) E553* probably null Het
Zfyve26 T C 12: 79,315,231 (GRCm39) D1415G possibly damaging Het
Zpbp2 C T 11: 98,442,141 (GRCm39) probably benign Het
Other mutations in Crlf1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02878:Crlf1 APN 8 70,956,290 (GRCm39) critical splice donor site probably null
R0317:Crlf1 UTSW 8 70,951,249 (GRCm39) missense probably benign
R0398:Crlf1 UTSW 8 70,951,739 (GRCm39) splice site probably benign
R0437:Crlf1 UTSW 8 70,952,164 (GRCm39) splice site probably null
R1191:Crlf1 UTSW 8 70,951,478 (GRCm39) missense probably damaging 1.00
R1741:Crlf1 UTSW 8 70,953,556 (GRCm39) missense probably damaging 0.99
R3730:Crlf1 UTSW 8 70,952,092 (GRCm39) missense probably benign 0.03
R3731:Crlf1 UTSW 8 70,952,092 (GRCm39) missense probably benign 0.03
R4467:Crlf1 UTSW 8 70,953,606 (GRCm39) nonsense probably null
R5557:Crlf1 UTSW 8 70,951,317 (GRCm39) missense probably benign 0.12
R6009:Crlf1 UTSW 8 70,956,129 (GRCm39) missense probably damaging 1.00
R6348:Crlf1 UTSW 8 70,945,990 (GRCm39) missense probably benign
R6606:Crlf1 UTSW 8 70,953,824 (GRCm39) missense probably damaging 1.00
R7947:Crlf1 UTSW 8 70,951,862 (GRCm39) missense probably damaging 1.00
R9313:Crlf1 UTSW 8 70,951,466 (GRCm39) missense probably damaging 1.00
X0062:Crlf1 UTSW 8 70,951,487 (GRCm39) splice site probably null
Predicted Primers PCR Primer
(F):5'- TGCCTCAGCTTCAATGAGTGC -3'
(R):5'- GCGTGACACACCAGATTGTCTC -3'

Sequencing Primer
(F):5'- CTTTATCTGTGAAATGGGTGAACAC -3'
(R):5'- TGACACACCAGATTGTCTCCTGAC -3'
Posted On 2022-04-18