Mutagenetix > Incidental Mutation

Incidental Mutation 'R9749:Lcn6'

732377

Institutional Source

Beutler Lab

Gene Symbol

Lcn6

Ensembl Gene

ENSMUSG00000045684

Gene Name

lipocalin 6

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R9749 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

25676786-25681608 bp(+) (GRCm38)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 25680022 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 1 (M1L)

Ref Sequence

ENSEMBL: ENSMUSP00000109835 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058912] [ENSMUST00000059693] [ENSMUST00000114197] [ENSMUST00000114199]

AlphaFold

A2AJB9

Predicted Effect

probably benign
Transcript: ENSMUST00000058912

SMART Domains

Protein: ENSMUSP00000059353
Gene: ENSMUSG00000047356

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:Lipocalin	36	169	1.6e-8	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000059693
AA Change: M1L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000055660
Gene: ENSMUSG00000045684
AA Change: M1L

Domain	Start	End	E-Value	Type
Pfam:Lipocalin	7	106	1.4e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114197
AA Change: M1L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000109835
Gene: ENSMUSG00000045684
AA Change: M1L

Domain	Start	End	E-Value	Type
Pfam:Lipocalin	7	106	4.8e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114199

SMART Domains

Protein: ENSMUSP00000109837
Gene: ENSMUSG00000045684

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:Lipocalin	33	172	2.6e-12	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.3%
20x: 98.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice with loss of expression in the testes show premature acrosome reaction and elevated intracellular calcium levels in sperm. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	T	A	11: 72,189,695	I382F	possibly damaging	Het
9030624J02Rik	C	A	7: 118,752,884	D195E	probably benign	Het
Adamtsl3	A	G	7: 82,450,186	S183G	probably benign	Het
Adamtsl4	T	C	3: 95,684,147	H170R	probably benign	Het
Adcy2	T	C	13: 68,625,855	D1023G	probably damaging	Het
Agrn	C	A	4: 156,173,657	V1083F	probably benign	Het
Arhgap21	T	C	2: 20,849,215	T1789A	probably benign	Het
Atr	T	C	9: 95,937,650		probably null	Het
Bcl2l13	T	C	6: 120,865,672	S109P	possibly damaging	Het
Bik	A	T	15: 83,541,339	T14S	possibly damaging	Het
Car7	A	G	8: 104,548,422	D164G	probably damaging	Het
Cat	T	A	2: 103,455,100	K499M	probably benign	Het
Ccar2	T	A	14: 70,151,279	Q161L	probably benign	Het
Cd109	T	G	9: 78,684,884	V760G	probably damaging	Het
Cep350	A	T	1: 155,953,239	H246Q	probably benign	Het
Chst13	T	C	6: 90,318,269	D54G	probably damaging	Het
Col4a4	A	G	1: 82,485,632	S1046P	unknown	Het
Col6a5	T	C	9: 105,861,991	Q2581R	probably damaging	Het
Csmd2	A	T	4: 128,496,128	I2219F		Het
Cux2	G	A	5: 121,869,717	S627L	possibly damaging	Het
Cyp2a22	A	T	7: 26,939,290	W24R	probably null	Het
Dhrs7c	A	T	11: 67,811,653	T156S	probably damaging	Het
Dnah9	A	G	11: 66,095,376	V1144A	probably damaging	Het
Folh1	T	C	7: 86,719,700	T740A	probably damaging	Het
Foxo4	G	A	X: 101,258,349	S209N	probably benign	Het
Gm10300	A	G	4: 132,074,718	T8A	unknown	Het
Gm11273	G	A	13: 21,501,345	A25V	probably benign	Het
Gns	T	A	10: 121,378,152	I244N	probably benign	Het
Gsdmd	A	G	15: 75,863,562	K52E	probably damaging	Het
H1f0	A	G	15: 79,029,017	D99G	probably benign	Het
Hapln4	T	C	8: 70,087,074	Y252H	probably damaging	Het
Hmcn1	A	C	1: 150,756,588	I866S	possibly damaging	Het
Hrnr	C	T	3: 93,324,077	R541C	unknown	Het
Ick	T	A	9: 78,153,717	V215E	probably damaging	Het
Igkv4-57-1	A	G	6: 69,544,424	I98T	probably damaging	Het
Itih3	T	A	14: 30,919,322	D253V	probably damaging	Het
Kansl1l	T	A	1: 66,721,811	E869D	probably damaging	Het
Kif13a	T	C	13: 46,760,751	Q502R	probably damaging	Het
Lama5	T	A	2: 180,183,640	Q2447L	probably benign	Het
Lrrtm1	T	C	6: 77,243,872	V104A	probably damaging	Het
Maats1	A	G	16: 38,308,125	I496T	probably damaging	Het
Map3k13	T	C	16: 21,921,831	L636P	probably benign	Het
Melk	T	A	4: 44,307,067	H74Q	possibly damaging	Het
Mixl1	A	T	1: 180,694,746	M190K	probably benign	Het
Mttp	A	G	3: 138,125,228	S27P	probably damaging	Het
Myh6	T	C	14: 54,953,486	I911V	probably damaging	Het
Ncapd3	T	A	9: 27,045,577	I262N	probably benign	Het
Ncapg2	C	A	12: 116,447,748	S1051*	probably null	Het
Nckap1	G	A	2: 80,571,224	Q39*	probably null	Het
Nemp1	A	G	10: 127,688,329	D76G	probably benign	Het
Olfr1083-ps	T	C	2: 86,607,565	E2G	probably benign	Het
Olfr1145	A	C	2: 87,809,858	K13Q	probably benign	Het
Olfr1239	T	A	2: 89,418,318	I32F	possibly damaging	Het
Olfr166	A	T	16: 19,487,363	H175L	possibly damaging	Het
Olfr434	G	A	6: 43,217,324	W137*	probably null	Het
Osbpl5	C	A	7: 143,695,571	V578L	probably benign	Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398		probably benign	Het
Rabl3	A	G	16: 37,556,828	D49G	possibly damaging	Het
Rngtt	A	G	4: 33,368,618	I384V	possibly damaging	Het
Robo1	T	C	16: 72,308,369		probably benign	Het
Rtcb	C	A	10: 85,949,589	R125L	probably damaging	Het
Rtn4rl2	C	A	2: 84,872,610	R206L	probably damaging	Het
Sbk2	A	T	7: 4,957,334	I279N	probably benign	Het
Scaf1	A	G	7: 45,007,152	S768P	unknown	Het
Slc6a9	A	G	4: 117,864,001	H316R	probably damaging	Het
Son	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	16: 91,656,691		probably benign	Het
Tas2r136	G	T	6: 132,778,143	T7K	probably damaging	Het
Tbc1d9	T	A	8: 83,241,710		probably null	Het
Tekt2	C	T	4: 126,323,651	R207H	probably damaging	Het
Tmem40	T	C	6: 115,742,345	D53G	probably benign	Het
Tram1	A	T	1: 13,570,014	V272D	possibly damaging	Het
Trpc4	G	A	3: 54,194,881	D67N	probably damaging	Het
Ttc6	A	T	12: 57,654,773	T591S	probably benign	Het
Ube2g1	A	T	11: 72,679,373	E144V	probably benign	Het
Unc80	A	G	1: 66,505,020	D434G	probably damaging	Het
Vmn1r168	C	A	7: 23,540,812	N31K	probably damaging	Het
Vmn1r79	A	T	7: 12,176,523	T111S	probably damaging	Het
Vmn2r118	C	T	17: 55,608,415		probably null	Het
Yy1	A	G	12: 108,806,491	D231G	possibly damaging	Het
Zfp748	A	G	13: 67,542,454	V229A	probably benign	Het

Other mutations in Lcn6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01086:Lcn6	APN	2	25680780	missense	probably benign	0.29
R0747:Lcn6	UTSW	2	25677172	missense	probably damaging	1.00
R1387:Lcn6	UTSW	2	25677137	missense	possibly damaging	0.86
R1500:Lcn6	UTSW	2	25677119	missense	probably benign	0.00
R1654:Lcn6	UTSW	2	25680775	splice site	probably null
R3005:Lcn6	UTSW	2	25677249	splice site	probably null
R4821:Lcn6	UTSW	2	25680810	missense	probably damaging	1.00
R4972:Lcn6	UTSW	2	25680067	missense	probably damaging	1.00
R5011:Lcn6	UTSW	2	25677070	splice site	probably null
R5013:Lcn6	UTSW	2	25677070	splice site	probably null
R7779:Lcn6	UTSW	2	25680793	missense	probably benign
R8034:Lcn6	UTSW	2	25676871	nonsense	probably null
R8558:Lcn6	UTSW	2	25680706	missense	probably damaging	1.00
R8697:Lcn6	UTSW	2	25677154	missense	probably benign	0.01
R9227:Lcn6	UTSW	2	25680095	missense	probably damaging	1.00
R9312:Lcn6	UTSW	2	25680062	missense	probably benign	0.23
R9740:Lcn6	UTSW	2	25681179	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GGATAGTGGCTCATCACCTG -3'
(R):5'- AGCAAAGTGGCCTCCTTTCC -3'

Sequencing Primer
(F):5'- GATAGTGGCTCATCACCTGAACTG -3'
(R):5'- CCTGTCAACTGCAGTGTGAGAATC -3'

Posted On

2022-11-14