Incidental Mutation 'R0606:Rev1'
ID 54117
Institutional Source Beutler Lab
Gene Symbol Rev1
Ensembl Gene ENSMUSG00000026082
Gene Name REV1, DNA directed polymerase
Synonyms 1110027I23Rik, Rev1l, REV1
MMRRC Submission 038795-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.921) question?
Stock # R0606 (G1)
Quality Score 155
Status Validated
Chromosome 1
Chromosomal Location 38091867-38168882 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 38098204 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Tryptophan at position 780 (R780W)
Ref Sequence ENSEMBL: ENSMUSP00000027251 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027251] [ENSMUST00000027252]
AlphaFold Q920Q2
PDB Structure Solution structure of the mouse Rev1 C-terminal domain [SOLUTION NMR]
Solution structure of the mouse Rev1 CTD in complex with the Rev1-interacting Region (RIR)of Pol Kappa [SOLUTION NMR]
Structure of the Rev1 CTD-Rev3/7-Pol kappa RIR complex [X-RAY DIFFRACTION]
Predicted Effect probably null
Transcript: ENSMUST00000027251
AA Change: R780W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000027251
Gene: ENSMUSG00000026082
AA Change: R780W

DomainStartEndE-ValueType
BRCT 46 121 3.99e-13 SMART
low complexity region 320 342 N/A INTRINSIC
Pfam:IMS 420 620 1.9e-43 PFAM
Pfam:IMS_C 700 831 5.8e-20 PFAM
low complexity region 888 901 N/A INTRINSIC
Pfam:DUF4414 938 1071 9.7e-11 PFAM
Pfam:REV1_C 1127 1248 1.2e-43 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000027252
SMART Domains Protein: ENSMUSP00000027252
Gene: ENSMUSG00000026083

DomainStartEndE-ValueType
low complexity region 19 32 N/A INTRINSIC
low complexity region 33 51 N/A INTRINSIC
low complexity region 94 106 N/A INTRINSIC
low complexity region 110 126 N/A INTRINSIC
low complexity region 145 161 N/A INTRINSIC
low complexity region 183 193 N/A INTRINSIC
coiled coil region 227 272 N/A INTRINSIC
coiled coil region 301 414 N/A INTRINSIC
low complexity region 480 498 N/A INTRINSIC
coiled coil region 523 554 N/A INTRINSIC
low complexity region 580 594 N/A INTRINSIC
Pfam:GTP_EFTU 625 840 4.7e-35 PFAM
Pfam:MMR_HSR1 629 753 5.1e-6 PFAM
Pfam:GTP_EFTU_D2 866 944 7.1e-11 PFAM
Pfam:IF-2 959 1066 1.4e-20 PFAM
Blast:S1 1116 1172 2e-6 BLAST
Predicted Effect noncoding transcript
Transcript: ENSMUST00000193008
Predicted Effect noncoding transcript
Transcript: ENSMUST00000193113
Predicted Effect noncoding transcript
Transcript: ENSMUST00000194650
Meta Mutation Damage Score 0.7221 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.8%
  • 10x: 97.3%
  • 20x: 94.2%
Validation Efficiency 98% (99/101)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
PHENOTYPE: Mice homozygous for a knock-in allele exhibit abnormal somatic hypermutation frequency of the Ig gene. Mice homozygous for a knock-out allele exhibit background-sensitive prenatal lethality and abnormal somatic hypermutation frequency. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 95 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actl9 T C 17: 33,652,572 (GRCm39) Y211H probably damaging Het
Actn1 A T 12: 80,221,421 (GRCm39) probably benign Het
Adtrp A G 13: 41,920,881 (GRCm39) F197L probably damaging Het
Ankrd11 G A 8: 123,619,571 (GRCm39) T1406I probably benign Het
Arhgap24 A T 5: 103,045,086 (GRCm39) R620W probably damaging Het
Atg13 A G 2: 91,512,418 (GRCm39) Y284H probably benign Het
Atrn A G 2: 130,748,776 (GRCm39) E99G possibly damaging Het
Cage1 A T 13: 38,200,470 (GRCm39) probably benign Het
Cblif A T 19: 11,729,658 (GRCm39) I206F possibly damaging Het
Ccr3 T A 9: 123,828,839 (GRCm39) M58K probably benign Het
Cdk18 G T 1: 132,045,355 (GRCm39) probably benign Het
Chst5 A G 8: 112,617,551 (GRCm39) V23A probably benign Het
Col4a3 T C 1: 82,650,307 (GRCm39) probably benign Het
Col4a6 A G X: 139,975,219 (GRCm39) probably benign Het
Csmd3 T C 15: 48,321,058 (GRCm39) I251V probably benign Het
Csnk1g3 G A 18: 54,050,100 (GRCm39) V115M probably damaging Het
Cst7 A T 2: 150,412,439 (GRCm39) M1L probably benign Het
Cyp4f17 A T 17: 32,746,817 (GRCm39) D373V probably damaging Het
Dclk2 G A 3: 86,813,311 (GRCm39) R212W probably damaging Het
Dhrs7b T G 11: 60,721,572 (GRCm39) probably benign Het
Dhx58 T A 11: 100,593,077 (GRCm39) H210L probably benign Het
Dnah9 T C 11: 65,732,159 (GRCm39) Y4249C probably damaging Het
Eif5b T A 1: 38,087,974 (GRCm39) L990H probably damaging Het
Faap24 T C 7: 35,094,388 (GRCm39) probably benign Het
Fryl T A 5: 73,282,077 (GRCm39) H174L probably benign Het
Gabrr1 T C 4: 33,132,696 (GRCm39) W15R probably benign Het
Gm15446 T C 5: 110,091,347 (GRCm39) V533A probably benign Het
Gm6760 T A X: 63,195,259 (GRCm39) K63* probably null Het
Gne C T 4: 44,042,244 (GRCm39) E444K possibly damaging Het
Gpr173 T A X: 151,130,036 (GRCm39) M146L possibly damaging Het
Hira C T 16: 18,753,797 (GRCm39) S547L probably benign Het
Hnf1b A G 11: 83,754,810 (GRCm39) H161R probably benign Het
Hnrnpm T A 17: 33,877,364 (GRCm39) N53I probably damaging Het
Hs3st5 A T 10: 36,708,584 (GRCm39) I40F probably benign Het
Hydin C T 8: 111,276,430 (GRCm39) probably benign Het
Ift172 A G 5: 31,411,657 (GRCm39) I1607T probably damaging Het
Igfn1 T C 1: 135,887,639 (GRCm39) Q2475R probably damaging Het
Il6st T C 13: 112,640,806 (GRCm39) S800P possibly damaging Het
Iqub G A 6: 24,501,260 (GRCm39) probably benign Het
Itgb1 A T 8: 129,448,853 (GRCm39) probably benign Het
Kctd21 G A 7: 96,996,808 (GRCm39) E94K probably benign Het
Kir3dl2 A G X: 135,354,260 (GRCm39) V233A possibly damaging Het
Klra2 A T 6: 131,197,187 (GRCm39) C271S probably damaging Het
Lacc1 A T 14: 77,267,061 (GRCm39) C401S probably damaging Het
Lmna T C 3: 88,389,885 (GRCm39) E580G probably damaging Het
Matn2 A G 15: 34,345,296 (GRCm39) Y101C probably damaging Het
Mrps16 G A 14: 20,441,457 (GRCm39) R116* probably null Het
Ndrg2 G T 14: 52,143,674 (GRCm39) R333S probably damaging Het
Nf2 A G 11: 4,732,194 (GRCm39) I507T possibly damaging Het
Nktr A G 9: 121,578,356 (GRCm39) probably benign Het
Nkx3-1 G A 14: 69,428,455 (GRCm39) probably benign Het
Npat T C 9: 53,467,781 (GRCm39) probably null Het
Nrxn1 T C 17: 90,872,801 (GRCm39) N1047S probably damaging Het
Nup210 A T 6: 91,003,911 (GRCm39) I1402N possibly damaging Het
Or5d40 G T 2: 88,015,624 (GRCm39) M134I possibly damaging Het
Parp14 G A 16: 35,677,130 (GRCm39) A946V probably benign Het
Pdcl2 C T 5: 76,460,328 (GRCm39) S182N probably benign Het
Pdia3 G A 2: 121,262,858 (GRCm39) G275S probably damaging Het
Pla2g4a A G 1: 149,716,455 (GRCm39) F669L probably benign Het
Plekha8 A G 6: 54,606,805 (GRCm39) K367E probably damaging Het
Pola1 A G X: 92,531,693 (GRCm39) probably benign Het
Ppm1d C T 11: 85,236,703 (GRCm39) T494I probably benign Het
Pramel16 T A 4: 143,676,453 (GRCm39) Y217F probably benign Het
Prl6a1 A T 13: 27,498,177 (GRCm39) probably benign Het
Ptprg T A 14: 12,154,131 (GRCm38) S617R probably benign Het
R3hdm2 G A 10: 127,280,313 (GRCm39) G45D probably damaging Het
Rnf139 T A 15: 58,771,676 (GRCm39) F567Y probably damaging Het
Scarf1 T C 11: 75,405,174 (GRCm39) V71A probably damaging Het
Shtn1 A G 19: 58,988,372 (GRCm39) S438P probably damaging Het
Slc30a3 T A 5: 31,246,067 (GRCm39) H221L probably benign Het
Smo A C 6: 29,753,603 (GRCm39) I160L possibly damaging Het
Snapc5 A T 9: 64,086,582 (GRCm39) probably benign Het
Snf8 G T 11: 95,925,799 (GRCm39) probably benign Het
Spata31d1a T C 13: 59,850,245 (GRCm39) S628G probably benign Het
Sphkap A T 1: 83,258,145 (GRCm39) D199E probably damaging Het
Spring1 T C 5: 118,397,154 (GRCm39) Y128H probably damaging Het
Stxbp5l T C 16: 37,024,883 (GRCm39) T572A possibly damaging Het
Thada C A 17: 84,723,731 (GRCm39) V1108L possibly damaging Het
Tln1 T C 4: 43,547,756 (GRCm39) Q735R probably benign Het
Trim24 G A 6: 37,848,169 (GRCm39) E42K probably benign Het
Trnt1 T A 6: 106,754,869 (GRCm39) probably benign Het
Ttbk2 A T 2: 120,604,353 (GRCm39) M215K probably damaging Het
Ttc8 C T 12: 98,909,718 (GRCm39) probably benign Het
Ube3c A G 5: 29,795,926 (GRCm39) Y105C probably damaging Het
Unc13c A G 9: 73,438,265 (GRCm39) probably benign Het
Usp36 A G 11: 118,153,854 (GRCm39) probably benign Het
Vcf2 C T X: 149,181,360 (GRCm39) A144T probably benign Het
Vmn2r102 T C 17: 19,899,106 (GRCm39) S483P possibly damaging Het
Wdr95 A G 5: 149,511,595 (GRCm39) T432A probably damaging Het
Wnk1 G T 6: 119,903,644 (GRCm39) P2523H probably damaging Het
Xpo4 A G 14: 57,875,665 (GRCm39) probably benign Het
Zar1 G T 5: 72,737,886 (GRCm39) P71Q probably damaging Het
Zbtb41 T C 1: 139,351,348 (GRCm39) Y154H probably benign Het
Zer1 G T 2: 29,994,809 (GRCm39) probably benign Het
Zfp454 A G 11: 50,765,012 (GRCm39) F140S probably benign Het
Other mutations in Rev1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00896:Rev1 APN 1 38,138,021 (GRCm39) missense probably damaging 1.00
IGL01065:Rev1 APN 1 38,138,090 (GRCm39) missense possibly damaging 0.89
IGL01393:Rev1 APN 1 38,131,144 (GRCm39) missense probably damaging 1.00
IGL03003:Rev1 APN 1 38,127,154 (GRCm39) missense possibly damaging 0.77
H8562:Rev1 UTSW 1 38,095,848 (GRCm39) missense probably damaging 0.96
PIT1430001:Rev1 UTSW 1 38,095,337 (GRCm39) unclassified probably benign
R0409:Rev1 UTSW 1 38,113,449 (GRCm39) nonsense probably null
R1134:Rev1 UTSW 1 38,096,768 (GRCm39) missense probably benign 0.04
R1171:Rev1 UTSW 1 38,127,581 (GRCm39) missense possibly damaging 0.89
R1208:Rev1 UTSW 1 38,098,199 (GRCm39) unclassified probably benign
R1440:Rev1 UTSW 1 38,127,286 (GRCm39) missense probably damaging 1.00
R1485:Rev1 UTSW 1 38,127,653 (GRCm39) missense probably benign 0.00
R1627:Rev1 UTSW 1 38,094,571 (GRCm39) missense probably damaging 0.99
R3845:Rev1 UTSW 1 38,138,069 (GRCm39) missense probably damaging 0.99
R3948:Rev1 UTSW 1 38,113,414 (GRCm39) missense possibly damaging 0.69
R4074:Rev1 UTSW 1 38,093,319 (GRCm39) missense possibly damaging 0.50
R4075:Rev1 UTSW 1 38,093,319 (GRCm39) missense possibly damaging 0.50
R4076:Rev1 UTSW 1 38,093,319 (GRCm39) missense possibly damaging 0.50
R4248:Rev1 UTSW 1 38,146,729 (GRCm39) missense possibly damaging 0.87
R4293:Rev1 UTSW 1 38,147,500 (GRCm39) missense possibly damaging 0.89
R4548:Rev1 UTSW 1 38,098,275 (GRCm39) missense possibly damaging 0.72
R4610:Rev1 UTSW 1 38,092,730 (GRCm39) missense probably damaging 1.00
R4654:Rev1 UTSW 1 38,118,337 (GRCm39) intron probably benign
R5032:Rev1 UTSW 1 38,113,570 (GRCm39) intron probably benign
R5286:Rev1 UTSW 1 38,094,407 (GRCm39) nonsense probably null
R5311:Rev1 UTSW 1 38,118,474 (GRCm39) missense probably benign 0.00
R5327:Rev1 UTSW 1 38,147,532 (GRCm39) nonsense probably null
R6363:Rev1 UTSW 1 38,110,570 (GRCm39) missense probably damaging 1.00
R7050:Rev1 UTSW 1 38,093,352 (GRCm39) missense probably damaging 1.00
R7072:Rev1 UTSW 1 38,106,626 (GRCm39) nonsense probably null
R7132:Rev1 UTSW 1 38,110,530 (GRCm39) missense possibly damaging 0.95
R7264:Rev1 UTSW 1 38,124,682 (GRCm39) missense probably damaging 1.00
R7298:Rev1 UTSW 1 38,092,185 (GRCm39) missense probably damaging 1.00
R7367:Rev1 UTSW 1 38,113,488 (GRCm39) nonsense probably null
R7395:Rev1 UTSW 1 38,127,146 (GRCm39) missense possibly damaging 0.69
R7829:Rev1 UTSW 1 38,095,526 (GRCm39) missense probably damaging 0.98
R8053:Rev1 UTSW 1 38,102,222 (GRCm39) missense possibly damaging 0.67
R8093:Rev1 UTSW 1 38,114,097 (GRCm39) intron probably benign
R8356:Rev1 UTSW 1 38,098,324 (GRCm39) nonsense probably null
R8456:Rev1 UTSW 1 38,098,324 (GRCm39) nonsense probably null
R8461:Rev1 UTSW 1 38,122,868 (GRCm39) missense possibly damaging 0.56
R8724:Rev1 UTSW 1 38,127,150 (GRCm39) missense probably damaging 1.00
R8757:Rev1 UTSW 1 38,098,353 (GRCm39) missense probably damaging 1.00
R8759:Rev1 UTSW 1 38,098,353 (GRCm39) missense probably damaging 1.00
R8945:Rev1 UTSW 1 38,122,824 (GRCm39) missense probably damaging 0.98
R9309:Rev1 UTSW 1 38,093,945 (GRCm39) missense probably damaging 1.00
R9433:Rev1 UTSW 1 38,092,173 (GRCm39) missense probably damaging 1.00
R9500:Rev1 UTSW 1 38,102,214 (GRCm39) nonsense probably null
X0017:Rev1 UTSW 1 38,092,742 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ATGTTCCTGACACTGGGATGCCAC -3'
(R):5'- ACCTTTGTGTAGCCCAAAGAAGCAG -3'

Sequencing Primer
(F):5'- TCTTACATCACCAGGAACTGTG -3'
(R):5'- GAAAATCATGGTGCGGAAGC -3'
Posted On 2013-07-11