Mutagenetix > Incidental Mutation

Incidental Mutation 'R0486:Or8b35'

42291

Institutional Source

Beutler Lab

Gene Symbol

Or8b35

Ensembl Gene

ENSMUSG00000060583

Gene Name

olfactory receptor family 8 subfamily B member 35

Synonyms

Olfr881, MOR162-7, GA_x6K02T2PVTD-31676771-31677700

MMRRC Submission

038685-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R0486 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

37903788-37904735 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 37903998 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 70 (N70S)

Ref Sequence

ENSEMBL: ENSMUSP00000074193 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074611] [ENSMUST00000212489]

AlphaFold

Q8VF63

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074611
AA Change: N70S

PolyPhen 2 Score 0.724 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000074193
Gene: ENSMUSG00000060583
AA Change: N70S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	36	311	4.6e-46	PFAM
Pfam:7tm_1	46	293	1.4e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000212489
AA Change: N65S

PolyPhen 2 Score 0.677 (Sensitivity: 0.86; Specificity: 0.92)

Meta Mutation Damage Score

0.1714

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 91.9%

Validation Efficiency

98% (65/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aco1	T	C	4: 40,177,783 (GRCm39)	L268P	probably damaging	Het
Adam22	A	T	5: 8,380,048 (GRCm39)	H83Q	probably damaging	Het
Anln	T	C	9: 22,264,122 (GRCm39)	D886G	probably benign	Het
Arhgef11	T	A	3: 87,596,159 (GRCm39)		probably null	Het
Ark2c	T	A	18: 77,571,950 (GRCm39)	Q91L	probably damaging	Het
Arl8b	A	T	6: 108,792,287 (GRCm39)	D116V	possibly damaging	Het
BC051665	C	T	13: 60,931,859 (GRCm39)	G180D	probably damaging	Het
Bloc1s2	A	G	19: 44,131,589 (GRCm39)		probably benign	Het
Ccdc185	T	G	1: 182,575,424 (GRCm39)	S422R	possibly damaging	Het
Cd101	T	C	3: 100,915,408 (GRCm39)	K720E	possibly damaging	Het
Cdh23	C	A	10: 60,222,725 (GRCm39)	A1236S	probably damaging	Het
Chd1	G	A	17: 15,954,604 (GRCm39)	A491T	probably damaging	Het
Chdh	T	C	14: 29,754,815 (GRCm39)	V275A	possibly damaging	Het
Cmtm2b	A	T	8: 105,057,047 (GRCm39)	I136F	probably damaging	Het
Cps1	T	C	1: 67,204,551 (GRCm39)	V457A	probably damaging	Het
Cwf19l1	A	G	19: 44,103,129 (GRCm39)	V362A	probably benign	Het
Cyp4f17	T	C	17: 32,743,797 (GRCm39)		probably benign	Het
Cyp4f18	C	A	8: 72,749,861 (GRCm39)	V263L	probably benign	Het
Dclre1a	A	G	19: 56,529,922 (GRCm39)		probably benign	Het
Dpp6	T	C	5: 27,866,640 (GRCm39)	I446T	probably benign	Het
F11r	T	C	1: 171,288,156 (GRCm39)	W61R	probably damaging	Het
Fam120b	C	T	17: 15,646,550 (GRCm39)		probably benign	Het
Fastkd2	T	C	1: 63,791,499 (GRCm39)	V669A	possibly damaging	Het
Foxg1	T	C	12: 49,431,314 (GRCm39)		probably benign	Het
Foxo3	A	G	10: 42,073,477 (GRCm39)	Y347H	probably damaging	Het
G3bp1	T	C	11: 55,389,452 (GRCm39)	F383L	probably damaging	Het
Gbp7	C	A	3: 142,252,078 (GRCm39)		probably benign	Het
Glipr1	T	C	10: 111,832,754 (GRCm39)		probably benign	Het
Gm11555	A	G	11: 99,540,986 (GRCm39)	S8P	unknown	Het
H6pd	G	A	4: 150,067,393 (GRCm39)		probably benign	Het
Haus8	C	T	8: 71,709,182 (GRCm39)	M75I	probably benign	Het
Haus8	C	A	8: 71,709,181 (GRCm39)	G76W	probably damaging	Het
Kcnj13	C	A	1: 87,314,752 (GRCm39)	V157L	probably damaging	Het
Kcnt2	T	A	1: 140,437,218 (GRCm39)	C550*	probably null	Het
Kdm5d	A	G	Y: 927,107 (GRCm39)	N615S	probably damaging	Het
Naip2	A	C	13: 100,298,290 (GRCm39)	I582S	probably benign	Het
Ncapd2	G	A	6: 125,160,990 (GRCm39)	R292*	probably null	Het
Ngef	T	A	1: 87,406,848 (GRCm39)	N640I	probably damaging	Het
Nhlrc3	T	C	3: 53,359,858 (GRCm39)	Y335C	probably damaging	Het
Nipbl	A	T	15: 8,368,354 (GRCm39)		probably benign	Het
Nop2	A	G	6: 125,117,636 (GRCm39)	K434R	probably null	Het
Nr4a3	T	C	4: 48,056,525 (GRCm39)		probably benign	Het
Piezo2	A	C	18: 63,162,132 (GRCm39)	I2233R	probably damaging	Het
Prag1	A	T	8: 36,613,787 (GRCm39)	E1113V	probably damaging	Het
Prpsap2	A	G	11: 61,631,826 (GRCm39)	I177T	possibly damaging	Het
Psmd1	T	A	1: 86,022,012 (GRCm39)	N611K	probably damaging	Het
Ptpn7	C	T	1: 135,065,096 (GRCm39)	T168I	probably damaging	Het
Pus1	A	T	5: 110,927,596 (GRCm39)	V53E	probably damaging	Het
Rgs22	A	G	15: 36,093,028 (GRCm39)	M415T	probably damaging	Het
Rnf17	C	T	14: 56,751,632 (GRCm39)	T1490M	probably benign	Het
Rnf20	C	A	4: 49,645,907 (GRCm39)	L332I	possibly damaging	Het
Snrnp40	C	G	4: 130,271,836 (GRCm39)		probably null	Het
Spam1	A	T	6: 24,796,394 (GRCm39)	Q115L	probably damaging	Het
Syce1l	A	T	8: 114,381,395 (GRCm39)		probably null	Het
Synj1	T	C	16: 90,735,151 (GRCm39)		probably benign	Het
Tas2r126	A	T	6: 42,412,225 (GRCm39)	I253F	probably benign	Het
Tecpr2	G	A	12: 110,862,803 (GRCm39)	V72I	probably benign	Het
Tfap2a	G	T	13: 40,882,170 (GRCm39)	P45Q	probably damaging	Het
Trip12	C	A	1: 84,738,805 (GRCm39)	G714*	probably null	Het
Wdr31	A	G	4: 62,372,130 (GRCm39)	S330P	probably damaging	Het
Wdr64	T	C	1: 175,622,769 (GRCm39)		probably benign	Het
Yes1	T	A	5: 32,812,926 (GRCm39)	Y343*	probably null	Het

Other mutations in Or8b35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01882:Or8b35	APN	9	37,903,856 (GRCm39)	missense	probably damaging	1.00
IGL02282:Or8b35	APN	9	37,904,318 (GRCm39)	missense	probably damaging	0.98
IGL02714:Or8b35	APN	9	37,904,382 (GRCm39)	missense	possibly damaging	0.83
R0308:Or8b35	UTSW	9	37,904,141 (GRCm39)	missense	probably benign	0.01
R0504:Or8b35	UTSW	9	37,904,438 (GRCm39)	missense	probably benign	0.05
R1939:Or8b35	UTSW	9	37,904,385 (GRCm39)	missense	probably benign	0.12
R4669:Or8b35	UTSW	9	37,904,381 (GRCm39)	missense	possibly damaging	0.83
R5377:Or8b35	UTSW	9	37,903,908 (GRCm39)	missense	probably benign	0.13
R5468:Or8b35	UTSW	9	37,904,307 (GRCm39)	missense	probably damaging	1.00
R5601:Or8b35	UTSW	9	37,904,010 (GRCm39)	missense	possibly damaging	0.79
R6799:Or8b35	UTSW	9	37,904,478 (GRCm39)	missense	possibly damaging	0.79
R7212:Or8b35	UTSW	9	37,904,253 (GRCm39)	missense	possibly damaging	0.47
R7648:Or8b35	UTSW	9	37,903,856 (GRCm39)	missense	probably damaging	1.00
R8286:Or8b35	UTSW	9	37,904,401 (GRCm39)	nonsense	probably null
X0064:Or8b35	UTSW	9	37,904,636 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- ATCCTGCTTGGCTTGACACAGC -3'
(R):5'- TGGCAGTATGCGCCATAGCTTC -3'

Sequencing Primer
(F):5'- TTGACACAGCAGCCAGAG -3'
(R):5'- GCTTACAGATGGCAGCATATC -3'

Posted On

2013-05-23