Incidental Mutation 'R5628:Gramd2'
ID441935
Institutional Source Beutler Lab
Gene Symbol Gramd2
Ensembl Gene ENSMUSG00000074259
Gene NameGRAM domain containing 2
Synonyms
MMRRC Submission 043167-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5628 (G1)
Quality Score225
Status Not validated
Chromosome9
Chromosomal Location59680144-59718874 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 59707723 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Threonine at position 3 (M3T)
Ref Sequence ENSEMBL: ENSMUSP00000116879 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000098661] [ENSMUST00000123914] [ENSMUST00000128944] [ENSMUST00000129129]
Predicted Effect probably benign
Transcript: ENSMUST00000098661
SMART Domains Protein: ENSMUSP00000096258
Gene: ENSMUSG00000074259

DomainStartEndE-ValueType
GRAM 66 133 2.93e-20 SMART
transmembrane domain 273 290 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000123914
SMART Domains Protein: ENSMUSP00000114530
Gene: ENSMUSG00000074259

DomainStartEndE-ValueType
GRAM 73 140 2.93e-20 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000128944
AA Change: M3T

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)
SMART Domains Protein: ENSMUSP00000116879
Gene: ENSMUSG00000074259
AA Change: M3T

DomainStartEndE-ValueType
GRAM 74 141 2.93e-20 SMART
transmembrane domain 281 298 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000129129
SMART Domains Protein: ENSMUSP00000118901
Gene: ENSMUSG00000074259

DomainStartEndE-ValueType
transmembrane domain 161 178 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000133822
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 95.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 44 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam25 A G 8: 40,755,710 D671G probably benign Het
Ap3b1 A G 13: 94,477,048 D685G unknown Het
Atp6v1h G A 1: 5,135,889 W358* probably null Het
Atr T A 9: 95,874,226 Y830* probably null Het
B3galnt2 A T 13: 13,995,152 probably null Het
Casz1 T C 4: 148,946,096 Y1191H probably damaging Het
Cdc40 T G 10: 40,851,053 E169D probably benign Het
Cep55 C T 19: 38,069,948 Q330* probably null Het
Clcn1 T C 6: 42,298,889 V315A probably damaging Het
Cmya5 A G 13: 93,089,710 F2957L probably damaging Het
Dnah2 C T 11: 69,458,920 R2399Q probably benign Het
Dync1li2 T C 8: 104,420,592 N490S possibly damaging Het
Ephb3 T C 16: 21,218,119 Y111H probably damaging Het
Fam186a A C 15: 99,941,747 H2205Q possibly damaging Het
Fat3 T C 9: 15,966,096 Y3407C probably damaging Het
Fbxw8 A G 5: 118,092,557 V393A probably damaging Het
Fnip1 A T 11: 54,503,633 D965V probably benign Het
Kctd15 T C 7: 34,640,295 D283G probably damaging Het
Kif9 C T 9: 110,514,553 R547* probably null Het
Map4 A G 9: 110,081,847 T245A probably benign Het
Mindy4 C T 6: 55,260,594 L385F probably damaging Het
Myo7b A G 18: 31,974,187 C1252R probably benign Het
Myt1l T A 12: 29,811,621 I134N unknown Het
Olfr1022 T A 2: 85,868,805 I71N probably damaging Het
Osgin2 T A 4: 15,998,998 N208I probably benign Het
Polr2b G A 5: 77,313,216 V29M probably damaging Het
Prdm15 T A 16: 97,799,623 M812L probably damaging Het
Prr36 TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC 8: 4,216,273 probably benign Het
Rev3l T A 10: 39,822,967 N1153K probably damaging Het
Rnf17 A G 14: 56,486,952 probably null Het
Rusc2 C T 4: 43,425,348 T1151M probably damaging Het
Scrib T C 15: 76,049,540 T30A possibly damaging Het
Sephs1 T C 2: 4,889,207 I73T probably benign Het
Sf3b1 C T 1: 54,998,175 A861T probably benign Het
Shq1 A G 6: 100,631,003 W316R probably damaging Het
Slc26a5 T A 5: 21,816,976 D484V probably benign Het
Smg1 C T 7: 118,154,701 probably benign Het
Stard5 T C 7: 83,633,147 I56T probably benign Het
Szt2 A G 4: 118,373,217 V2653A unknown Het
Tmem217 A T 17: 29,526,456 I100N probably damaging Het
Trpm2 C T 10: 77,912,636 R1400Q probably benign Het
Vmn1r82 T G 7: 12,305,278 N41K probably damaging Het
Zfp236 T C 18: 82,657,122 D367G probably damaging Het
Zfyve1 A T 12: 83,574,889 V244E probably benign Het
Other mutations in Gramd2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01682:Gramd2 APN 9 59712110 missense possibly damaging 0.76
IGL03032:Gramd2 APN 9 59712127 missense probably benign 0.22
R5119:Gramd2 UTSW 9 59714320 intron probably benign
R5703:Gramd2 UTSW 9 59708016 missense probably benign 0.25
R5730:Gramd2 UTSW 9 59711206 missense probably damaging 1.00
R5799:Gramd2 UTSW 9 59708016 missense probably benign 0.25
R7424:Gramd2 UTSW 9 59708071 missense possibly damaging 0.75
R7531:Gramd2 UTSW 9 59709910 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TGAGCAAGTCTGTGTCCTTCC -3'
(R):5'- TTAGCTGCACTGTGTCCAG -3'

Sequencing Primer
(F):5'- TCCTTCCTCCTGGGTGGG -3'
(R):5'- CTCTACCCTTTGATGTACAGGG -3'
Posted On2016-11-08