Mutagenetix > Incidental Mutation

Incidental Mutation 'FR4340:Zfp28'

511123

Institutional Source

Beutler Lab

Gene Symbol

Zfp28

Ensembl Gene

ENSMUSG00000062861

Gene Name

zinc finger protein 28

Synonyms

2810438M17Rik, mkr-5, Zfp-28

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

FR4340 ()

Quality Score

80.0075

Status

Not validated

Chromosome

Chromosomal Location

6386317-6399636 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 6397862 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 766 (G766R)

Ref Sequence

ENSEMBL: ENSMUSP00000079812 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081022] [ENSMUST00000161855]

AlphaFold

P10078

Predicted Effect

probably damaging
Transcript: ENSMUST00000081022
AA Change: G766R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000079812
Gene: ENSMUSG00000062861
AA Change: G766R

Domain	Start	End	E-Value	Type
low complexity region	27	50	N/A	INTRINSIC
KRAB	103	163	3.53e-33	SMART
ZnF_C2H2	377	399	3.95e-4	SMART
ZnF_C2H2	405	427	6.88e-4	SMART
ZnF_C2H2	433	456	1.2e-3	SMART
ZnF_C2H2	462	484	9.58e-3	SMART
ZnF_C2H2	490	512	2.57e-3	SMART
ZnF_C2H2	518	540	1.82e-3	SMART
ZnF_C2H2	546	568	2.4e-3	SMART
ZnF_C2H2	574	596	6.32e-3	SMART
ZnF_C2H2	602	624	1.38e-3	SMART
ZnF_C2H2	630	652	4.87e-4	SMART
ZnF_C2H2	658	680	2.91e-2	SMART
ZnF_C2H2	686	708	2.36e-2	SMART
ZnF_C2H2	714	736	6.42e-4	SMART
ZnF_C2H2	742	764	2.4e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159318

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160568

Predicted Effect

probably benign
Transcript: ENSMUST00000161855

SMART Domains

Protein: ENSMUSP00000130202
Gene: ENSMUSG00000093536

Domain	Start	End	E-Value	Type
low complexity region	57	72	N/A	INTRINSIC
transmembrane domain	85	107	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000207086

Predicted Effect

probably benign
Transcript: ENSMUST00000207465

Predicted Effect

probably benign
Transcript: ENSMUST00000207809

Predicted Effect

probably benign
Transcript: ENSMUST00000208949

Predicted Effect

probably benign
Transcript: ENSMUST00000208338

Coding Region Coverage

1x: 99.7%
3x: 99.4%
10x: 97.8%
20x: 94.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 115 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	AACC	A	7: 40,642,479 (GRCm39)		probably benign	Het
4930578G10Rik	G	T	4: 42,761,098 (GRCm39)		probably benign	Het
7530416G11Rik	T	A	15: 85,378,508 (GRCm39)	E45V	unknown	Homo
A530064D06Rik	GTAGGAAGCTTAG	GTAG	17: 48,470,549 (GRCm39)		probably benign	Homo
Arpc1b	CC	CCTGGTC	5: 145,063,602 (GRCm39)		probably null	Het
Arrb2	C	T	11: 70,329,497 (GRCm39)	T269M	probably damaging	Homo
Bcas3	G	A	11: 85,400,323 (GRCm39)	V431I	probably benign	Homo
Blm	ACCT	ACCTGCCT	7: 80,113,515 (GRCm39)		probably benign	Het
Blm	TCCTCCTCCTCCTCCTCCTCCTCC	TCCTCCTCCTCCACCTCCTCCTCCTCCTCCTCCTCC	7: 80,162,658 (GRCm39)		probably benign	Het
Blm	TCCTCCTCCTCCTCCTCCTCCTCC	TCCTCCTCCTCCGCCTCCTCCTCCTCCTCCTCCTCC	7: 80,162,655 (GRCm39)		probably benign	Het
Bltp1	TATTATTAT	TATTATTATTATTATCATTATTAT	3: 37,104,901 (GRCm39)		probably benign	Het
Cacna1a	ACC	ACCGCC	8: 85,365,352 (GRCm39)		probably benign	Het
Cacna1f	AGG	AGGCGG	X: 7,486,306 (GRCm39)		probably benign	Het
Calhm1	CTCTGTGGCTGTGGCTGTGGCTGTG	CTCTGTGGCTGTGTCTGTGGCTGTGGCTGTGGCTGTG	19: 47,129,690 (GRCm39)		probably benign	Het
Casz1	ACCACAGCCACAGCCACAGCCAC	ACCACAGCCACAGCCAC	4: 149,036,759 (GRCm39)		probably benign	Homo
Ccdc121	GAGAAG	GAG	5: 31,644,717 (GRCm39)		probably benign	Het
Cd164	G	T	10: 41,397,922 (GRCm39)	A59S	probably benign	Het
Cd22	C	T	7: 30,577,507 (GRCm39)	R2H	possibly damaging	Homo
Cd80	GAA	GAAAAA	16: 38,306,678 (GRCm39)		probably benign	Homo
Cimip2b	CAGAG	CAG	4: 43,427,384 (GRCm39)		probably null	Homo
Col2a1	C	A	15: 97,886,862 (GRCm39)		probably null	Het
Col6a5	A	T	9: 105,811,373 (GRCm39)	N715K	unknown	Homo
Crygc	A	T	1: 65,110,822 (GRCm39)	F155Y	probably benign	Het
Cul9	TCC	TCCCCC	17: 46,811,779 (GRCm39)		probably benign	Het
Cyp2d11	T	TGGGA	15: 82,274,223 (GRCm39)		probably null	Homo
Dbr1	AGG	AGGAGGCGG	9: 99,465,754 (GRCm39)		probably benign	Het
Dennd10	CT	CTTTT	19: 60,803,059 (GRCm39)		probably benign	Homo
Dennd2b	CACCACACTGGGGCAGCCCACACTGGGGCAG	CCCCACACTGGGGCAG	7: 109,156,128 (GRCm39)		probably benign	Het
Dnah12	G	T	14: 26,571,342 (GRCm39)	G2817V	probably damaging	Homo
Dnah8	ACACTGCC	AC	17: 30,854,437 (GRCm39)		probably benign	Het
Dthd1	C	CTTA	5: 63,000,369 (GRCm39)		probably benign	Homo
Frem3	CT	CTTTT	8: 81,341,870 (GRCm39)		probably benign	Homo
Frmpd2	G	T	14: 33,232,978 (GRCm39)	L399F	probably damaging	Homo
G530012D18Rik	CACACAGAGAGAGAGAGAGAGAGAGA	CA	1: 85,504,873 (GRCm39)		probably benign	Het
Gbp2b	A	G	3: 142,309,413 (GRCm39)	I175V	probably benign	Het
Gm14393	T	C	2: 174,903,427 (GRCm39)	E160G	possibly damaging	Het
Gm16519	A	AGAAC	17: 71,236,333 (GRCm39)		probably null	Homo
Gm4340	GCAG	GCAACAG	10: 104,031,959 (GRCm39)		probably benign	Het
Gm4340	CAGAAG	CAGAAGAAG	10: 104,031,960 (GRCm39)		probably benign	Het
Gm4340	CAG	CAGTAG	10: 104,031,936 (GRCm39)		probably null	Het
Gpatch11	AGGAAG	AGGAAGGGGAAG	17: 79,149,603 (GRCm39)		probably benign	Het
H2-Q4	G	A	17: 35,599,381 (GRCm39)	D155N	probably damaging	Het
Ifi208	ATGGTG	ATG	1: 173,505,264 (GRCm39)		probably benign	Homo
Ighv5-9	C	T	12: 113,625,497 (GRCm39)	S82N	probably benign	Homo
Ipo9	TCC	TCCCCC	1: 135,314,007 (GRCm39)		probably benign	Het
Ipo9	CTC	CTCTTC	1: 135,314,009 (GRCm39)		probably benign	Het
Isg20l2	AAG	AAGTAG	3: 87,839,019 (GRCm39)		probably null	Het
Kmt2b	TCCTCC	TCCTCCCCCTCC	7: 30,285,800 (GRCm39)		probably benign	Het
Kmt2b	TCCTCC	TCCTCCCCCTCC	7: 30,285,788 (GRCm39)		probably benign	Het
Kmt2b	TCCTCC	TCCTCCCCCTCC	7: 30,285,794 (GRCm39)		probably benign	Het
Krt10	CCTCCT	CCTCCTTCTCCT	11: 99,280,100 (GRCm39)		probably benign	Het
Krt10	CAC	CACGAC	11: 99,277,028 (GRCm39)		probably benign	Het
Krt10	ACCG	ACCGCCG	11: 99,277,029 (GRCm39)		probably benign	Homo
Las1l	TCCTC	TCCTCTACCTC	X: 94,984,228 (GRCm39)		probably benign	Het
Lce1a1	C	T	3: 92,554,151 (GRCm39)	G108S	unknown	Het
Lkaaear1	CCAGCTCCAG	CCAGCTCCAGCTGCAGCTCCAG	2: 181,339,387 (GRCm39)		probably benign	Het
Lrit3	CTG	CTGTTG	3: 129,582,457 (GRCm39)		probably benign	Het
Mamld1	CAG	CAGAAG	X: 70,162,452 (GRCm39)		probably benign	Het
Mapk7	TGCTGGCGCTGGTGCTGGCGCTGG	TGCTGGCGCTGGCGCTGGTGCTGGCGCTGG	11: 61,381,032 (GRCm39)		probably benign	Het
Mast4	GCA	GCAGTGTCA	13: 102,872,825 (GRCm39)		probably benign	Homo
Mast4	TTTT	TTTTATTT	13: 102,871,365 (GRCm39)		probably null	Het
Med12l	AGC	AGCCGC	3: 59,183,406 (GRCm39)		probably benign	Het
Mfsd5	G	A	15: 102,189,596 (GRCm39)	V323I	probably benign	Het
Nacad	GTC	GTCAGGATC	11: 6,549,761 (GRCm39)		probably benign	Het
Naip1	A	C	13: 100,559,584 (GRCm39)	M1140R	probably benign	Het
Nbea	TTTA	T	3: 55,916,633 (GRCm39)		probably benign	Homo
Nefh	CTCACCTGGGGACTTGGCCTC	CTCACCTGGGGACTTGGCCTCACCTGGGGACTTGGCCTC	11: 4,891,040 (GRCm39)		probably benign	Homo
Nefh	ACTTGGCCTCACCTGGGG	ACTTGGCCTCACCTGGGGCCTTGGCCTCACCTGGGG	11: 4,891,033 (GRCm39)		probably benign	Het
Nefh	GCCTCACCTGGGGACTTGGCCTC	GCCTCACCTGGGGACTTGGCCTCACCTGGGGACTTGGCCTC	11: 4,891,038 (GRCm39)		probably benign	Homo
Neu1	TCTTCTA	T	17: 35,151,534 (GRCm39)		probably benign	Het
Nutf2	G	T	8: 106,603,202 (GRCm39)	D78Y	probably damaging	Het
Or51q1	TCC	TCCC	7: 103,629,110 (GRCm39)		probably null	Het
Or5e1	AT	ATGATATT	7: 108,354,161 (GRCm39)		probably benign	Homo
Or5p70	A	G	7: 107,995,100 (GRCm39)	T258A	probably benign	Het
Or5p70	G	A	7: 107,995,105 (GRCm39)	M259I	probably benign	Het
Pdik1l	ACCAC	ACCACCCCCAC	4: 134,006,823 (GRCm39)		probably benign	Het
Phaf1	G	A	8: 105,967,730 (GRCm39)	G207E	probably benign	Homo
Pik3c2g	AG	AGAGGG	6: 139,612,654 (GRCm39)		probably null	Homo
Plekhs1	TCCAGAC	TCCAGACCTCCCCCCAGAC	19: 56,468,290 (GRCm39)		probably benign	Homo
Prag1	C	CAGT	8: 36,571,040 (GRCm39)		probably benign	Homo
Pramel16	G	A	4: 143,676,312 (GRCm39)	T264M	probably damaging	Het
Prkn	G	A	17: 12,073,650 (GRCm39)	V323M	probably damaging	Homo
Raet1d	A	G	10: 22,247,458 (GRCm39)	Q178R	probably benign	Het
Serac1	T	A	17: 6,121,083 (GRCm39)	K70N	probably damaging	Homo
Serpina3i	CGG	CGGTGG	12: 104,231,423 (GRCm39)		probably benign	Het
Sfswap	ACTCAGCCC	ACTCAGCCCCCTCAGCCC	5: 129,646,815 (GRCm39)		probably benign	Het
Six3	CGG	CGGGGG	17: 85,928,784 (GRCm39)		probably benign	Het
Sp140l1	A	C	1: 85,087,072 (GRCm39)	N6K	probably damaging	Het
Speer4a1	C	A	5: 26,241,746 (GRCm39)	E127*	probably null	Het
Sry	GCTGCTGCTGCTG	GCTGCTGCTGCTGCTG	Y: 2,662,824 (GRCm39)		probably benign	Het
Tbr1	A	C	2: 61,636,691 (GRCm39)		probably benign	Het
Tdpoz2	T	TCC	3: 93,558,922 (GRCm39)		probably null	Homo
Tdpoz4	GAA	GA	3: 93,704,187 (GRCm39)		probably null	Het
Tgoln1	AAG	AAGCCTCAG	6: 72,593,334 (GRCm39)		probably benign	Homo
Tmbim7	C	T	5: 3,720,064 (GRCm39)	R100C	possibly damaging	Het
Tob1	CA	CAGAA	11: 94,105,303 (GRCm39)		probably benign	Het
Tob1	AGC	AGCCGC	11: 94,105,280 (GRCm39)		probably benign	Het
Tob1	AGC	AGCCGC	11: 94,105,286 (GRCm39)		probably benign	Het
Tomm5	GCATCTTCC	GCATCTTCCACATCTTCC	4: 45,107,973 (GRCm39)		probably benign	Het
Triobp	TCGG	TCGGCGG	15: 78,877,590 (GRCm39)		probably benign	Homo
Tsbp1	GC	GCAAC	17: 34,679,051 (GRCm39)		probably benign	Het
Tsbp1	CAG	CAGTAG	17: 34,679,034 (GRCm39)		probably null	Het
Tsbp1	GCA	GCATCA	17: 34,679,042 (GRCm39)		probably benign	Het
Tsen2	AGG	AGGGGG	6: 115,537,027 (GRCm39)		probably benign	Homo
Tsen2	AGG	AGGGGG	6: 115,537,030 (GRCm39)		probably benign	Homo
Ubtf	TCC	TCCCCC	11: 102,197,776 (GRCm39)		probably benign	Het
Vmn2r87	C	T	10: 130,314,583 (GRCm39)	M334I	probably benign	Homo
Zc3h13	CGGGATGTGCG	CGGGATGTGCGGGATGTGCG	14: 75,561,032 (GRCm39)		probably benign	Homo
Zfp384	AAGCCCAGGCCCAGGCCCAGGCCCA	AAGCCCAGGCCCAAGCCCAGGCCCAGGCCCAGGCCCA	6: 125,013,426 (GRCm39)		probably benign	Het
Zfp428	G	A	7: 24,214,506 (GRCm39)	D41N	probably damaging	Homo
Zfp598	CCACAGGC	CC	17: 24,898,346 (GRCm39)		probably benign	Het
Zfp598	CCACCA	CCACCAACACCA	17: 24,899,757 (GRCm39)		probably benign	Het
Zfp831	TCC	TCCCCC	2: 174,487,273 (GRCm39)		probably benign	Het
Zfp933	GCTT	GCTTTTCTT	4: 147,910,186 (GRCm39)		probably null	Homo
Zfp936	G	A	7: 42,838,913 (GRCm39)	G127R	possibly damaging	Het

Other mutations in Zfp28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00737:Zfp28	APN	7	6,396,429 (GRCm39)	makesense	probably null
IGL02300:Zfp28	APN	7	6,392,495 (GRCm39)	missense	probably benign	0.00
IGL02541:Zfp28	APN	7	6,396,479 (GRCm39)	nonsense	probably null
FR4342:Zfp28	UTSW	7	6,397,862 (GRCm39)	missense	probably damaging	1.00
R0442:Zfp28	UTSW	7	6,397,998 (GRCm39)	missense	probably damaging	1.00
R0462:Zfp28	UTSW	7	6,395,239 (GRCm39)	missense	possibly damaging	0.71
R0799:Zfp28	UTSW	7	6,387,182 (GRCm39)	missense	possibly damaging	0.49
R1081:Zfp28	UTSW	7	6,392,779 (GRCm39)	missense	possibly damaging	0.93
R1674:Zfp28	UTSW	7	6,397,942 (GRCm39)	missense	possibly damaging	0.90
R1783:Zfp28	UTSW	7	6,397,791 (GRCm39)	missense	probably damaging	1.00
R2119:Zfp28	UTSW	7	6,397,875 (GRCm39)	missense	probably benign	0.00
R2186:Zfp28	UTSW	7	6,397,497 (GRCm39)	missense	probably damaging	1.00
R4280:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4281:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4283:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4331:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4379:Zfp28	UTSW	7	6,396,441 (GRCm39)	missense	probably benign	0.11
R4380:Zfp28	UTSW	7	6,396,441 (GRCm39)	missense	probably benign	0.11
R4505:Zfp28	UTSW	7	6,397,160 (GRCm39)	missense	probably damaging	1.00
R4659:Zfp28	UTSW	7	6,396,506 (GRCm39)	missense	probably benign	0.05
R4706:Zfp28	UTSW	7	6,392,793 (GRCm39)	missense	probably damaging	0.99
R5524:Zfp28	UTSW	7	6,397,850 (GRCm39)	splice site	probably null
R6269:Zfp28	UTSW	7	6,396,612 (GRCm39)	missense	probably benign	0.00
R6981:Zfp28	UTSW	7	6,397,692 (GRCm39)	missense	probably damaging	1.00
R7117:Zfp28	UTSW	7	6,397,461 (GRCm39)	missense	probably damaging	1.00
R7176:Zfp28	UTSW	7	6,386,456 (GRCm39)	missense	possibly damaging	0.49
R7312:Zfp28	UTSW	7	6,386,593 (GRCm39)	unclassified	probably benign
R7422:Zfp28	UTSW	7	6,397,748 (GRCm39)	missense	probably damaging	1.00
R7423:Zfp28	UTSW	7	6,396,956 (GRCm39)	missense	probably damaging	1.00
R7937:Zfp28	UTSW	7	6,396,785 (GRCm39)	missense	probably damaging	1.00
R8110:Zfp28	UTSW	7	6,392,828 (GRCm39)	missense	probably benign	0.02
R8704:Zfp28	UTSW	7	6,397,637 (GRCm39)	missense	probably damaging	1.00
R8804:Zfp28	UTSW	7	6,393,399 (GRCm39)	missense	probably damaging	0.98
R8854:Zfp28	UTSW	7	6,397,938 (GRCm39)	missense	probably benign	0.01
R9071:Zfp28	UTSW	7	6,397,544 (GRCm39)	missense	probably damaging	1.00
R9235:Zfp28	UTSW	7	6,397,605 (GRCm39)	missense	probably damaging	1.00
R9276:Zfp28	UTSW	7	6,397,440 (GRCm39)	missense	probably damaging	1.00
R9589:Zfp28	UTSW	7	6,392,816 (GRCm39)	missense	probably benign	0.09
R9600:Zfp28	UTSW	7	6,397,917 (GRCm39)	missense	probably benign	0.00
R9651:Zfp28	UTSW	7	6,395,623 (GRCm39)	missense
R9653:Zfp28	UTSW	7	6,395,623 (GRCm39)	missense
R9712:Zfp28	UTSW	7	6,396,878 (GRCm39)	missense	probably damaging	1.00
Y4340:Zfp28	UTSW	7	6,397,656 (GRCm39)	missense	probably damaging	1.00
Z1177:Zfp28	UTSW	7	6,387,186 (GRCm39)	missense	probably benign	0.28

Predicted Primers

PCR Primer
(F):5'- GGCCTTTGGTGATAACTCATCC -3'
(R):5'- ATGGCAGTGAGGATGGATTC -3'

Sequencing Primer
(F):5'- TGTACTCAACATCGGAGACTTC -3'
(R):5'- TGGATTCCAGACGAATTTGGAG -3'

Posted On

2018-04-05