Mutagenetix > Incidental Mutation

Incidental Mutation 'R8673:Zfp958'

661264

Institutional Source

Beutler Lab

Gene Symbol

Zfp958

Ensembl Gene

ENSMUSG00000058748

Gene Name

zinc finger protein 958

Synonyms

BC003267

MMRRC Submission

068528-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R8673 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

4663167-4680231 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 4678268 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 98 (T98S)

Ref Sequence

ENSEMBL: ENSMUSP00000143842 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073201] [ENSMUST00000202692]

AlphaFold

Q99LG7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000073201
AA Change: T97S

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: T97S

Domain	Start	End	E-Value	Type
KRAB	3	65	1.96e-17	SMART
ZnF_C2H2	102	124	2.67e-1	SMART
ZnF_C2H2	130	152	8.47e-4	SMART
ZnF_C2H2	158	180	5.5e-3	SMART
ZnF_C2H2	186	208	1.38e-3	SMART
ZnF_C2H2	214	236	9.96e-1	SMART
ZnF_C2H2	242	264	2.15e-5	SMART
ZnF_C2H2	270	292	3.16e-3	SMART
ZnF_C2H2	298	320	1.69e-3	SMART
ZnF_C2H2	326	348	5.14e-3	SMART
ZnF_C2H2	354	376	2.91e-2	SMART
ZnF_C2H2	382	404	2.27e-4	SMART
ZnF_C2H2	410	432	4.79e-3	SMART
ZnF_C2H2	438	460	6.88e-4	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000202692
AA Change: T98S

PolyPhen 2 Score 0.536 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: T98S

Domain	Start	End	E-Value	Type
KRAB	4	66	1.96e-17	SMART
ZnF_C2H2	103	125	2.67e-1	SMART
ZnF_C2H2	131	153	8.47e-4	SMART
ZnF_C2H2	159	181	5.5e-3	SMART
ZnF_C2H2	187	209	1.38e-3	SMART
ZnF_C2H2	215	237	9.96e-1	SMART
ZnF_C2H2	243	265	2.15e-5	SMART
ZnF_C2H2	271	293	3.16e-3	SMART
ZnF_C2H2	299	321	1.69e-3	SMART
ZnF_C2H2	327	349	5.14e-3	SMART
ZnF_C2H2	355	377	2.91e-2	SMART
ZnF_C2H2	383	405	2.27e-4	SMART
ZnF_C2H2	411	433	4.79e-3	SMART
ZnF_C2H2	439	461	6.88e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700067P10Rik	G	T	17: 48,400,849 (GRCm39)	E45*	probably null	Het
Anxa9	C	T	3: 95,207,657 (GRCm39)	R250Q	probably benign	Het
Apaf1	A	G	10: 90,831,530 (GRCm39)	S1195P	probably damaging	Het
Baiap2l1	T	A	5: 144,212,852 (GRCm39)		probably benign	Het
Bpifb6	G	T	2: 153,747,212 (GRCm39)	C172F	probably damaging	Het
Ccdc9	A	T	7: 16,018,286 (GRCm39)	D7E	probably damaging	Het
Cdcp3	A	T	7: 130,844,846 (GRCm39)	I580F	probably damaging	Het
Chchd6	T	A	6: 89,546,380 (GRCm39)	S109C	probably damaging	Het
Cltc	G	A	11: 86,648,201 (GRCm39)		probably benign	Het
Col4a6	G	A	X: 140,017,173 (GRCm39)	P60L	probably damaging	Het
Cxxc1	C	T	18: 74,351,915 (GRCm39)	T264I	probably benign	Het
D5Ertd579e	A	G	5: 36,830,151 (GRCm39)	S58P	probably benign	Het
Dlgap1	T	A	17: 71,122,293 (GRCm39)	H907Q	probably damaging	Het
Dnah2	A	T	11: 69,405,523 (GRCm39)	M663K	probably benign	Het
Dock8	G	A	19: 25,160,867 (GRCm39)	M1791I	probably damaging	Het
Esd	T	C	14: 74,969,952 (GRCm39)	F8S	probably benign	Het
Etnk2	T	C	1: 133,302,300 (GRCm39)	Y273H	probably damaging	Het
Fry	C	A	5: 150,318,576 (GRCm39)	H935Q	possibly damaging	Het
Gabrr3	T	G	16: 59,235,633 (GRCm39)	M45R	possibly damaging	Het
Gata4	T	C	14: 63,478,258 (GRCm39)	T114A	probably benign	Het
Gcnt4	G	A	13: 97,082,997 (GRCm39)	D98N	probably benign	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Igkv1-99	C	T	6: 68,519,387 (GRCm39)	Q115*	probably null	Het
Irak3	G	T	10: 119,982,493 (GRCm39)	T323K	possibly damaging	Het
Lrp2	T	C	2: 69,302,804 (GRCm39)	D2975G	probably damaging	Het
Mybph	T	A	1: 134,126,142 (GRCm39)	W319R	probably damaging	Het
Nell1	A	G	7: 49,869,343 (GRCm39)	D206G	probably damaging	Het
Or10ag55-ps1	A	T	2: 87,114,658 (GRCm39)	D8V	probably benign	Het
Or4c105	T	C	2: 88,647,590 (GRCm39)	V25A	probably benign	Het
Or5b114-ps1	T	C	19: 13,352,581 (GRCm39)	I85T	probably benign	Het
Pex1	A	C	5: 3,685,886 (GRCm39)	D1209A	possibly damaging	Het
Phf10	T	C	17: 15,170,868 (GRCm39)	D355G	probably benign	Het
Psmd2	T	C	16: 20,475,638 (GRCm39)	S413P	probably damaging	Het
Ptgir	G	T	7: 16,641,287 (GRCm39)	C193F	probably damaging	Het
Rad21l	A	G	2: 151,502,718 (GRCm39)	I164T	possibly damaging	Het
Ralgapb	G	A	2: 158,292,133 (GRCm39)	R773H	probably damaging	Het
Rasgef1b	G	T	5: 99,370,844 (GRCm39)	N385K	probably damaging	Het
Shank3	C	T	15: 89,433,979 (GRCm39)	R1575C	probably damaging	Het
Slc7a2	G	T	8: 41,365,446 (GRCm39)		probably benign	Het
Spata1	T	A	3: 146,181,079 (GRCm39)	N293I	probably damaging	Het
Spen	C	T	4: 141,197,681 (GRCm39)	A3396T	probably benign	Het
Sphkap	T	C	1: 83,253,561 (GRCm39)	H1396R	probably benign	Het
Spire2	A	G	8: 124,086,867 (GRCm39)	E446G	probably damaging	Het
Tdpoz2	A	T	3: 93,558,918 (GRCm39)	C351*	probably null	Het
Thoc5	G	A	11: 4,876,061 (GRCm39)	V605I	possibly damaging	Het
Ttll2	C	T	17: 7,619,340 (GRCm39)	V196I	possibly damaging	Het
Uba6	A	G	5: 86,284,178 (GRCm39)	Y576H	probably damaging	Het
Vegfb	G	A	19: 6,962,812 (GRCm39)	P188L	unknown	Het
Vmn1r62	A	G	7: 5,678,277 (GRCm39)		probably benign	Het
Vmn2r73	T	A	7: 85,521,902 (GRCm39)	T146S	probably benign	Het
Wdr64	A	G	1: 175,633,584 (GRCm39)	Y945C	probably damaging	Het
Zfp870	C	T	17: 33,101,904 (GRCm39)	C475Y	probably damaging	Het

Other mutations in Zfp958

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01950:Zfp958	APN	8	4,678,917 (GRCm39)	missense	probably damaging	1.00
IGL02185:Zfp958	APN	8	4,678,990 (GRCm39)	nonsense	probably null
IGL02716:Zfp958	APN	8	4,675,967 (GRCm39)	critical splice donor site	probably null
R1118:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1119:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1478:Zfp958	UTSW	8	4,679,190 (GRCm39)	missense	probably damaging	1.00
R1816:Zfp958	UTSW	8	4,679,147 (GRCm39)	missense	possibly damaging	0.89
R1837:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R1838:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R3763:Zfp958	UTSW	8	4,676,226 (GRCm39)	critical splice donor site	probably null
R5257:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5258:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5304:Zfp958	UTSW	8	4,676,196 (GRCm39)	missense	possibly damaging	0.73
R5512:Zfp958	UTSW	8	4,675,838 (GRCm39)	critical splice acceptor site	probably null
R5907:Zfp958	UTSW	8	4,679,072 (GRCm39)	missense	probably benign	0.01
R6604:Zfp958	UTSW	8	4,678,245 (GRCm39)	missense	probably damaging	1.00
R6688:Zfp958	UTSW	8	4,678,940 (GRCm39)	missense	possibly damaging	0.91
R6727:Zfp958	UTSW	8	4,678,247 (GRCm39)	missense	probably benign	0.08
R6981:Zfp958	UTSW	8	4,676,170 (GRCm39)	missense	probably benign	0.00
R7010:Zfp958	UTSW	8	4,678,377 (GRCm39)	missense	probably benign	0.13
R7140:Zfp958	UTSW	8	4,678,481 (GRCm39)	missense	probably benign	0.05
R8096:Zfp958	UTSW	8	4,663,273 (GRCm39)	start gained	probably benign
R8491:Zfp958	UTSW	8	4,676,215 (GRCm39)	missense	probably damaging	0.99
R8847:Zfp958	UTSW	8	4,678,434 (GRCm39)	missense	probably damaging	1.00
R9612:Zfp958	UTSW	8	4,678,298 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCAGTGTGGTAAGGTTCTAAGTTCT -3'
(R):5'- TTATGATGTGCAAAGGCTTTACCAC -3'

Sequencing Primer
(F):5'- GGTATCTTCAAAGATGCAAAGCAAC -3'
(R):5'- GCAAAGGCTTTACCACATTGATTAC -3'

Posted On

2021-03-08