Incidental Mutation 'R6688:Zfp958'
| ID |
527862 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp958
|
| Ensembl Gene |
ENSMUSG00000058748 |
| Gene Name |
zinc finger protein 958 |
| Synonyms |
BC003267 |
| MMRRC Submission |
044806-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.080)
|
| Stock # |
R6688 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
8 |
| Chromosomal Location |
4663167-4680231 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 4678940 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 322
(T322A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000143842
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000073201]
[ENSMUST00000202692]
|
| AlphaFold |
Q99LG7 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000073201
AA Change: T321A
PolyPhen 2
Score 0.444 (Sensitivity: 0.89; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: T321A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
3 |
65 |
1.96e-17 |
SMART |
|
ZnF_C2H2
|
102 |
124 |
2.67e-1 |
SMART |
|
ZnF_C2H2
|
130 |
152 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
158 |
180 |
5.5e-3 |
SMART |
|
ZnF_C2H2
|
186 |
208 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
214 |
236 |
9.96e-1 |
SMART |
|
ZnF_C2H2
|
242 |
264 |
2.15e-5 |
SMART |
|
ZnF_C2H2
|
270 |
292 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
298 |
320 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
326 |
348 |
5.14e-3 |
SMART |
|
ZnF_C2H2
|
354 |
376 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
382 |
404 |
2.27e-4 |
SMART |
|
ZnF_C2H2
|
410 |
432 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
438 |
460 |
6.88e-4 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000202692
AA Change: T322A
PolyPhen 2
Score 0.909 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: T322A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
4 |
66 |
1.96e-17 |
SMART |
|
ZnF_C2H2
|
103 |
125 |
2.67e-1 |
SMART |
|
ZnF_C2H2
|
131 |
153 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
159 |
181 |
5.5e-3 |
SMART |
|
ZnF_C2H2
|
187 |
209 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
215 |
237 |
9.96e-1 |
SMART |
|
ZnF_C2H2
|
243 |
265 |
2.15e-5 |
SMART |
|
ZnF_C2H2
|
271 |
293 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
299 |
321 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
327 |
349 |
5.14e-3 |
SMART |
|
ZnF_C2H2
|
355 |
377 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
383 |
405 |
2.27e-4 |
SMART |
|
ZnF_C2H2
|
411 |
433 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
439 |
461 |
6.88e-4 |
SMART |
|
| Meta Mutation Damage Score |
0.1168 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.7%
- 10x: 98.5%
- 20x: 95.8%
|
| Validation Efficiency |
100% (33/33) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 30 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Atxn1 |
A |
T |
13: 45,721,147 (GRCm39) |
H249Q |
probably damaging |
Het |
| Cd22 |
A |
C |
7: 30,572,389 (GRCm39) |
S362A |
possibly damaging |
Het |
| Cep120 |
G |
A |
18: 53,857,608 (GRCm39) |
P286S |
probably benign |
Het |
| Ces1g |
G |
A |
8: 94,033,600 (GRCm39) |
P441S |
possibly damaging |
Het |
| Ces2h |
A |
G |
8: 105,744,472 (GRCm39) |
I316V |
probably benign |
Het |
| Cntnap5c |
T |
A |
17: 58,600,899 (GRCm39) |
D747E |
possibly damaging |
Het |
| Cwf19l2 |
T |
C |
9: 3,450,015 (GRCm39) |
V572A |
probably benign |
Het |
| Cyb5rl |
C |
T |
4: 106,931,102 (GRCm39) |
A128V |
probably damaging |
Het |
| Dnttip1 |
T |
C |
2: 164,607,081 (GRCm39) |
Y241H |
probably damaging |
Het |
| Gm2381 |
G |
A |
7: 42,470,010 (GRCm39) |
A38V |
probably benign |
Het |
| Golga4 |
A |
G |
9: 118,343,278 (GRCm39) |
T11A |
possibly damaging |
Het |
| Ip6k2 |
C |
A |
9: 108,683,210 (GRCm39) |
T440K |
probably benign |
Het |
| Kif5c |
A |
G |
2: 49,578,749 (GRCm39) |
N126D |
probably benign |
Het |
| Mdn1 |
T |
A |
4: 32,774,041 (GRCm39) |
F5551I |
possibly damaging |
Het |
| Myh11 |
A |
G |
16: 14,023,417 (GRCm39) |
L1587P |
probably damaging |
Het |
| Nherf4 |
C |
T |
9: 44,159,527 (GRCm39) |
|
probably null |
Het |
| Nop53 |
A |
G |
7: 15,679,779 (GRCm39) |
V67A |
possibly damaging |
Het |
| Or1o1 |
G |
A |
17: 37,716,796 (GRCm39) |
R119H |
probably benign |
Het |
| Paxip1 |
T |
C |
5: 27,949,135 (GRCm39) |
T1045A |
probably benign |
Het |
| Plg |
A |
T |
17: 12,610,732 (GRCm39) |
H215L |
probably damaging |
Het |
| Psmb5 |
C |
A |
14: 54,854,130 (GRCm39) |
R116L |
probably damaging |
Het |
| Rapgef2 |
T |
A |
3: 78,976,435 (GRCm39) |
Q1307L |
probably benign |
Het |
| Serpini2 |
T |
C |
3: 75,166,870 (GRCm39) |
E129G |
possibly damaging |
Het |
| Stx1b |
C |
T |
7: 127,407,068 (GRCm39) |
R209Q |
probably damaging |
Het |
| Tcf20 |
A |
G |
15: 82,738,736 (GRCm39) |
I905T |
possibly damaging |
Het |
| Tmem252 |
G |
A |
19: 24,651,463 (GRCm39) |
A11T |
probably benign |
Het |
| Tpst2 |
A |
G |
5: 112,455,623 (GRCm39) |
N54S |
probably benign |
Het |
| Usp31 |
T |
C |
7: 121,277,553 (GRCm39) |
S269G |
probably benign |
Het |
| Wasf1 |
T |
C |
10: 40,802,616 (GRCm39) |
|
probably null |
Het |
| Zfp429 |
A |
T |
13: 67,544,249 (GRCm39) |
V58D |
probably damaging |
Het |
|
| Other mutations in Zfp958 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01950:Zfp958
|
APN |
8 |
4,678,917 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02185:Zfp958
|
APN |
8 |
4,678,990 (GRCm39) |
nonsense |
probably null |
|
|
IGL02716:Zfp958
|
APN |
8 |
4,675,967 (GRCm39) |
critical splice donor site |
probably null |
|
|
R1118:Zfp958
|
UTSW |
8 |
4,676,169 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R1119:Zfp958
|
UTSW |
8 |
4,676,169 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R1478:Zfp958
|
UTSW |
8 |
4,679,190 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1816:Zfp958
|
UTSW |
8 |
4,679,147 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R1837:Zfp958
|
UTSW |
8 |
4,678,590 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1838:Zfp958
|
UTSW |
8 |
4,678,590 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3763:Zfp958
|
UTSW |
8 |
4,676,226 (GRCm39) |
critical splice donor site |
probably null |
|
|
R5257:Zfp958
|
UTSW |
8 |
4,678,456 (GRCm39) |
missense |
probably benign |
0.28 |
|
R5258:Zfp958
|
UTSW |
8 |
4,678,456 (GRCm39) |
missense |
probably benign |
0.28 |
|
R5304:Zfp958
|
UTSW |
8 |
4,676,196 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R5512:Zfp958
|
UTSW |
8 |
4,675,838 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R5907:Zfp958
|
UTSW |
8 |
4,679,072 (GRCm39) |
missense |
probably benign |
0.01 |
|
R6604:Zfp958
|
UTSW |
8 |
4,678,245 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6727:Zfp958
|
UTSW |
8 |
4,678,247 (GRCm39) |
missense |
probably benign |
0.08 |
|
R6981:Zfp958
|
UTSW |
8 |
4,676,170 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7010:Zfp958
|
UTSW |
8 |
4,678,377 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7140:Zfp958
|
UTSW |
8 |
4,678,481 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8096:Zfp958
|
UTSW |
8 |
4,663,273 (GRCm39) |
start gained |
probably benign |
|
|
R8491:Zfp958
|
UTSW |
8 |
4,676,215 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8673:Zfp958
|
UTSW |
8 |
4,678,268 (GRCm39) |
missense |
possibly damaging |
0.54 |
|
R8847:Zfp958
|
UTSW |
8 |
4,678,434 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9612:Zfp958
|
UTSW |
8 |
4,678,298 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTTGCATGTCTCAGCTATCTTCGA -3'
(R):5'- ATCATATGGAAAGGCTTTACCACA -3'
Sequencing Primer
(F):5'- CAGCTATCTTCGAGTGCATGGAAG -3'
(R):5'- GCCACATTGATTACATCTGAAGGG -3'
|
| Posted On |
2018-07-23 |
Incidental Mutation