Mutagenetix > Incidental Mutation

Incidental Mutation 'R8736:Zfp870'

663047

Institutional Source

Beutler Lab

Gene Symbol

Zfp870

Ensembl Gene

ENSMUSG00000095325

Gene Name

zinc finger protein 870

Synonyms

MMRRC Submission

068617-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R8736 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

33098193-33110616 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 33104966 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 31 (V31E)

Ref Sequence

ENSEMBL: ENSMUSP00000136197 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178401] [ENSMUST00000228075]

AlphaFold

Q6NZJ3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000178401
AA Change: V31E

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000136197
Gene: ENSMUSG00000095325
AA Change: V31E

Domain	Start	End	E-Value	Type
KRAB	3	60	1.01e-19	SMART
ZnF_C2H2	193	215	4.5e1	SMART
ZnF_C2H2	221	241	6.4e0	SMART
ZnF_C2H2	277	299	2.24e-3	SMART
ZnF_C2H2	305	327	9.58e-3	SMART
ZnF_C2H2	333	355	2.86e-1	SMART
ZnF_C2H2	361	383	6.42e-4	SMART
ZnF_C2H2	389	411	1.18e-2	SMART
ZnF_C2H2	417	439	3.89e-3	SMART
ZnF_C2H2	445	467	5.5e-3	SMART
ZnF_C2H2	473	495	4.47e-3	SMART
ZnF_C2H2	501	523	5.9e-3	SMART
ZnF_C2H2	529	551	3.11e-2	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000228075
AA Change: V32E

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

Meta Mutation Damage Score

0.7131

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.9%
20x: 99.8%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bltp2	T	A	11: 78,178,875 (GRCm39)	S2047T	probably benign	Het
Ccdc125	T	C	13: 100,815,833 (GRCm39)	S100P	possibly damaging	Het
Ccnl1	T	C	3: 65,865,447 (GRCm39)	T35A	unknown	Het
Cul2	T	A	18: 3,434,019 (GRCm39)	V672E	probably damaging	Het
Cyb5a	C	A	18: 84,869,560 (GRCm39)		probably benign	Het
Ddhd1	T	A	14: 45,836,642 (GRCm39)	Y831F	probably benign	Het
Ddr1	A	G	17: 35,995,104 (GRCm39)	I698T	probably damaging	Het
Elovl7	A	T	13: 108,393,320 (GRCm39)	I18F	probably benign	Het
Fcgbp	G	T	7: 27,805,621 (GRCm39)	V1967L	probably benign	Het
Gm40460	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,450 (GRCm39)		probably benign	Het
Gtf2h3	A	G	5: 124,728,972 (GRCm39)	N177S	probably damaging	Het
Krt6b	A	G	15: 101,587,047 (GRCm39)	Y242H	probably damaging	Het
Mab21l2	C	A	3: 86,454,607 (GRCm39)	R131L	probably damaging	Het
Maml1	T	C	11: 50,148,726 (GRCm39)	T1005A	possibly damaging	Het
Mapk4	T	C	18: 74,103,396 (GRCm39)	T38A	probably benign	Het
Mapkapk5	G	A	5: 121,665,241 (GRCm39)	A338V	possibly damaging	Het
Morc2a	C	A	11: 3,631,737 (GRCm39)	T556K	probably damaging	Het
Muc16	G	A	9: 18,462,139 (GRCm39)	Q7459*	probably null	Het
Muc6	T	C	7: 141,228,439 (GRCm39)	T1256A	possibly damaging	Het
Or1e21	T	A	11: 73,344,384 (GRCm39)	Y218F	probably damaging	Het
Or4b12	A	G	2: 90,095,922 (GRCm39)	V284A	possibly damaging	Het
Or5a1	A	G	19: 12,097,309 (GRCm39)	C256R	probably damaging	Het
Or7g20	G	T	9: 18,946,774 (GRCm39)	M118I	probably damaging	Het
Or9i14	T	C	19: 13,792,358 (GRCm39)	I199V	probably benign	Het
Or9s18	T	G	13: 65,300,538 (GRCm39)	C167G	probably damaging	Het
Pik3c2a	A	G	7: 115,975,464 (GRCm39)	V701A	possibly damaging	Het
Plxnb2	A	T	15: 89,046,261 (GRCm39)	I918N	probably damaging	Het
Pms1	T	C	1: 53,307,053 (GRCm39)	S118G	possibly damaging	Het
Ppp1r18	A	G	17: 36,184,711 (GRCm39)	T549A	probably benign	Het
Rap1gds1	T	C	3: 138,647,512 (GRCm39)	I559V	probably benign	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Tenm4	C	T	7: 96,555,148 (GRCm39)	P2618S	probably benign	Het
Tlx1	A	G	19: 45,141,975 (GRCm39)	T200A	possibly damaging	Het
Usp1	A	G	4: 98,821,105 (GRCm39)	I488V	probably damaging	Het
Vmn2r6	T	A	3: 64,467,221 (GRCm39)	M93L	probably damaging	Het

Other mutations in Zfp870

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01304:Zfp870	APN	17	33,101,980 (GRCm39)	missense	possibly damaging	0.72
R1591:Zfp870	UTSW	17	33,102,990 (GRCm39)	missense	probably damaging	1.00
R1892:Zfp870	UTSW	17	33,102,863 (GRCm39)	missense	possibly damaging	0.66
R1941:Zfp870	UTSW	17	33,101,778 (GRCm39)	missense	possibly damaging	0.71
R1997:Zfp870	UTSW	17	33,103,027 (GRCm39)	missense	possibly damaging	0.66
R2289:Zfp870	UTSW	17	33,102,334 (GRCm39)	missense	probably benign	0.27
R4240:Zfp870	UTSW	17	33,104,710 (GRCm39)	missense	probably benign	0.00
R4860:Zfp870	UTSW	17	33,102,314 (GRCm39)	nonsense	probably null
R4860:Zfp870	UTSW	17	33,102,314 (GRCm39)	nonsense	probably null
R5306:Zfp870	UTSW	17	33,102,627 (GRCm39)	missense	probably damaging	1.00
R6425:Zfp870	UTSW	17	33,102,045 (GRCm39)	missense	possibly damaging	0.66
R6736:Zfp870	UTSW	17	33,102,570 (GRCm39)	missense	probably benign
R7054:Zfp870	UTSW	17	33,102,456 (GRCm39)	missense	probably damaging	0.97
R7291:Zfp870	UTSW	17	33,102,828 (GRCm39)	missense	probably damaging	0.97
R7466:Zfp870	UTSW	17	33,102,736 (GRCm39)	missense	possibly damaging	0.66
R7681:Zfp870	UTSW	17	33,101,664 (GRCm39)	missense	probably benign
R8230:Zfp870	UTSW	17	33,102,663 (GRCm39)	missense	possibly damaging	0.46
R8346:Zfp870	UTSW	17	33,102,843 (GRCm39)	missense	possibly damaging	0.92
R8673:Zfp870	UTSW	17	33,101,904 (GRCm39)	missense	probably damaging	0.97
R8695:Zfp870	UTSW	17	33,102,679 (GRCm39)	missense	possibly damaging	0.46
R9057:Zfp870	UTSW	17	33,102,793 (GRCm39)	missense	probably benign	0.27

Predicted Primers

PCR Primer
(F):5'- CCCGACTTGTTTCCTAAAATGC -3'
(R):5'- CGACAACTTGAACCCTTGTGG -3'

Sequencing Primer
(F):5'- CGACTTGTTTCCTAAAATGCAGACAC -3'
(R):5'- GAACCCTTGTGGAACATAGTTTCG -3'

Posted On

2021-03-08