Mutagenetix > Incidental Mutation

Incidental Mutation 'R9019:Zfp933'

686596

Institutional Source

Beutler Lab

Gene Symbol

Zfp933

Ensembl Gene

ENSMUSG00000059423

Gene Name

zinc finger protein 933

Synonyms

2810408P10Rik

MMRRC Submission

068849-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9019 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147907443-147932823 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 147911021 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 192 (C192S)

Ref Sequence

ENSEMBL: ENSMUSP00000101343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105718] [ENSMUST00000135798]

AlphaFold

Q6PEE4

Predicted Effect

probably damaging
Transcript: ENSMUST00000105718
AA Change: C192S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101343
Gene: ENSMUSG00000059423
AA Change: C192S

Domain	Start	End	E-Value	Type
KRAB	4	66	9.49e-16	SMART
ZnF_C2H2	131	153	3.21e-4	SMART
ZnF_C2H2	159	181	5.21e-4	SMART
ZnF_C2H2	187	209	2.12e-4	SMART
ZnF_C2H2	215	237	7.26e-3	SMART
ZnF_C2H2	243	265	6.88e-4	SMART
ZnF_C2H2	271	293	1.13e-4	SMART
ZnF_C2H2	299	321	3.95e-4	SMART
ZnF_C2H2	327	349	1.56e-2	SMART
ZnF_C2H2	355	377	1.79e-2	SMART
ZnF_C2H2	383	405	4.24e-4	SMART
ZnF_C2H2	411	433	1.22e-4	SMART
ZnF_C2H2	439	461	4.79e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135798

SMART Domains

Protein: ENSMUSP00000118300
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	8e-18	BLAST
PDB:2I13\|B	32	98	1e-12	PDB
SCOP:d1fgja_	33	98	5e-13	SMART
Blast:PHD	44	98	6e-11	BLAST

Meta Mutation Damage Score

0.9069

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca9	T	A	11: 110,011,522 (GRCm39)	T1174S		Het
Aoc2	C	T	11: 101,216,262 (GRCm39)	P115L	possibly damaging	Het
Arhgef19	C	T	4: 140,973,738 (GRCm39)	P75L	probably benign	Het
Atad3a	A	G	4: 155,838,052 (GRCm39)	V240A	possibly damaging	Het
AW551984	A	T	9: 39,508,973 (GRCm39)	L317*	probably null	Het
Bdkrb1	T	C	12: 105,570,700 (GRCm39)	S89P	probably damaging	Het
Bpifb4	T	A	2: 153,790,607 (GRCm39)	L166*	probably null	Het
Ccdc27	A	G	4: 154,124,014 (GRCm39)	V173A	unknown	Het
Ccl8	T	C	11: 82,006,877 (GRCm39)	V30A	probably benign	Het
Cdc16	C	T	8: 13,831,501 (GRCm39)	A578V	probably benign	Het
Cnksr1	T	C	4: 133,959,365 (GRCm39)	Y423C	probably damaging	Het
Cyp3a44	T	C	5: 145,727,519 (GRCm39)	D270G	probably damaging	Het
Ep300	A	G	15: 81,532,730 (GRCm39)	E1656G	unknown	Het
Eppk1	A	G	15: 75,992,472 (GRCm39)	F1470L	probably benign	Het
Fbxw16	T	A	9: 109,270,135 (GRCm39)	D202V	probably damaging	Het
Fndc3a	G	A	14: 72,811,840 (GRCm39)	T330I	probably benign	Het
Gm36864	A	T	7: 43,887,028 (GRCm39)	Q307L	probably benign	Het
Gm37240	T	C	3: 84,426,946 (GRCm39)	E38G	probably damaging	Het
Gm6871	A	T	7: 41,195,262 (GRCm39)	C492S	probably damaging	Het
Gnaq	T	C	19: 16,355,638 (GRCm39)	Y285H	probably benign	Het
Htt	T	A	5: 35,023,920 (GRCm39)	F1723I	probably damaging	Het
Il21r	G	A	7: 125,231,472 (GRCm39)	S300N	probably damaging	Het
Itm2b	G	A	14: 73,605,856 (GRCm39)		probably benign	Het
Kat6a	T	C	8: 23,425,754 (GRCm39)	S1100P	probably damaging	Het
Kcnj2	T	C	11: 110,963,415 (GRCm39)	I269T	probably damaging	Het
Kmt2c	G	A	5: 25,488,208 (GRCm39)	A4635V	probably damaging	Het
Kng2	A	T	16: 22,847,546 (GRCm39)	D38E	probably damaging	Het
Lgr5	A	C	10: 115,314,454 (GRCm39)	L161R	probably damaging	Het
Lrig2	A	T	3: 104,368,914 (GRCm39)	I923N	probably benign	Het
Mrgprh	T	C	17: 13,096,200 (GRCm39)	S147P	probably damaging	Het
Mthfd1	C	T	12: 76,350,754 (GRCm39)	T712M	probably benign	Het
Nsmaf	A	T	4: 6,418,523 (GRCm39)	S427R	probably damaging	Het
Or8g53	G	T	9: 39,684,038 (GRCm39)	D19E	probably benign	Het
Pdcd11	G	T	19: 47,101,658 (GRCm39)	G948C	probably damaging	Het
Pdzd2	A	G	15: 12,375,612 (GRCm39)	Y1508H	probably damaging	Het
Prmt5	G	T	14: 54,753,564 (GRCm39)	A105E	probably benign	Het
Prr23a3	T	C	9: 98,747,213 (GRCm39)	S56P	probably damaging	Het
Slc44a2	A	G	9: 21,265,077 (GRCm39)	E705G	probably damaging	Het
Sostdc1	A	T	12: 36,364,431 (GRCm39)	N47Y	probably benign	Het
Speg	T	C	1: 75,405,882 (GRCm39)	Y3029H	probably damaging	Het
Srd5a1	G	A	13: 69,748,413 (GRCm39)	R129*	probably null	Het
Srrm4	A	G	5: 116,605,586 (GRCm39)	S224P	unknown	Het
Syne2	A	G	12: 75,999,618 (GRCm39)	E2337G	possibly damaging	Het
Tmem150c	C	A	5: 100,240,958 (GRCm39)	V32L	probably benign	Het
Topaz1	T	C	9: 122,619,192 (GRCm39)	I1263T	possibly damaging	Het
Ttc39d	C	T	17: 80,523,349 (GRCm39)	R3W	probably benign	Het
Xylt1	T	C	7: 117,250,038 (GRCm39)		probably null	Het
Zswim8	G	A	14: 20,761,119 (GRCm39)	G129D	probably damaging	Het

Other mutations in Zfp933

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00576:Zfp933	APN	4	147,910,778 (GRCm39)	missense	probably damaging	1.00
IGL03377:Zfp933	APN	4	147,913,168 (GRCm39)	missense	possibly damaging	0.65
F5770:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
FR4340:Zfp933	UTSW	4	147,910,186 (GRCm39)	frame shift	probably null
FR4548:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
R0388:Zfp933	UTSW	4	147,910,899 (GRCm39)	missense	probably benign	0.35
R0523:Zfp933	UTSW	4	147,910,919 (GRCm39)	nonsense	probably null
R0539:Zfp933	UTSW	4	147,911,005 (GRCm39)	missense	probably benign	0.08
R1672:Zfp933	UTSW	4	147,910,476 (GRCm39)	missense	probably damaging	1.00
R4049:Zfp933	UTSW	4	147,910,969 (GRCm39)	missense	probably damaging	1.00
R4895:Zfp933	UTSW	4	147,910,892 (GRCm39)	nonsense	probably null
R5133:Zfp933	UTSW	4	147,911,321 (GRCm39)	missense	probably benign
R5786:Zfp933	UTSW	4	147,912,864 (GRCm39)	splice site	probably null
R5891:Zfp933	UTSW	4	147,911,231 (GRCm39)	missense	probably benign	0.03
R6111:Zfp933	UTSW	4	147,913,217 (GRCm39)	missense	probably damaging	1.00
R6382:Zfp933	UTSW	4	147,910,325 (GRCm39)	missense	probably benign	0.07
R6968:Zfp933	UTSW	4	147,910,654 (GRCm39)	missense	probably damaging	1.00
R7195:Zfp933	UTSW	4	147,910,636 (GRCm39)	missense	probably benign	0.16
R7555:Zfp933	UTSW	4	147,910,589 (GRCm39)	missense	probably damaging	1.00
R7902:Zfp933	UTSW	4	147,911,058 (GRCm39)	missense	probably damaging	0.96
R8319:Zfp933	UTSW	4	147,912,910 (GRCm39)	missense	possibly damaging	0.87
R8688:Zfp933	UTSW	4	147,911,249 (GRCm39)	missense	probably benign	0.14
RF024:Zfp933	UTSW	4	147,910,898 (GRCm39)	missense	probably damaging	1.00
RF028:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
RF035:Zfp933	UTSW	4	147,910,188 (GRCm39)	makesense	probably null
RF043:Zfp933	UTSW	4	147,910,188 (GRCm39)	frame shift	probably null
V7581:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7582:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98
V7583:Zfp933	UTSW	4	147,910,927 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGCGCAAAGGCTTTATCACATTG -3'
(R):5'- TGTGCCAATTATCTCTGCAGG -3'

Sequencing Primer
(F):5'- CGCAAAGGCTTTATCACATTGATTGC -3'
(R):5'- TATCTCTGCAGGCATGAAAGAAGTCC -3'

Posted On

2021-11-19