Incidental Mutation 'R9114:Mknk2'
ID 692427
Institutional Source Beutler Lab
Gene Symbol Mknk2
Ensembl Gene ENSMUSG00000020190
Gene Name MAP kinase-interacting serine/threonine kinase 2
Synonyms Mnk2, 2010016G11Rik
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R9114 (G1)
Quality Score 225.009
Status Not validated
Chromosome 10
Chromosomal Location 80501152-80512264 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 80504823 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Tyrosine at position 236 (N236Y)
Ref Sequence ENSEMBL: ENSMUSP00000143508 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000003433] [ENSMUST00000197276] [ENSMUST00000198819] [ENSMUST00000199949] [ENSMUST00000200082]
AlphaFold Q8CDB0
Predicted Effect probably damaging
Transcript: ENSMUST00000003433
AA Change: N189Y

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000003433
Gene: ENSMUSG00000020190
AA Change: N189Y

DomainStartEndE-ValueType
low complexity region 13 23 N/A INTRINSIC
S_TKc 36 321 7.09e-88 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000197276
SMART Domains Protein: ENSMUSP00000143679
Gene: ENSMUSG00000020190

DomainStartEndE-ValueType
SCOP:d1koba_ 52 118 3e-11 SMART
PDB:2AC3|A 59 118 3e-32 PDB
Blast:S_TKc 71 118 1e-24 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000198819
Predicted Effect probably benign
Transcript: ENSMUST00000199949
Predicted Effect probably damaging
Transcript: ENSMUST00000200082
AA Change: N236Y

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000143508
Gene: ENSMUSG00000020190
AA Change: N236Y

DomainStartEndE-ValueType
low complexity region 60 70 N/A INTRINSIC
S_TKc 83 368 7.09e-88 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.5%
Validation Efficiency
MGI Phenotype FUNCTION: The protein encoded by this gene is a serine/threonine-protein kinase, which is targeted by both the extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways. This enzyme targets several substrates including eukaryotic translation initiation factor 4E and mammalian target of rapamycin, which are negatively regulated by its phosphorylation. Null mutant mice do not exhibit developmental or reproductive defects. However, mice null for both this protein and mitogen-activated protein kinase-interacting serine/threonine protein kinase 1 have delayed tumor development in phosphatase and tensin homolog mutant mice, indicating an oncogenic function for this gene in tumor development. [provided by RefSeq, Oct 2014]
PHENOTYPE: Homozygous null mice are viable and fertile with no gross abnormalities. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1a T A 5: 8,788,702 (GRCm39) L1027* probably null Het
Actl11 T A 9: 107,808,509 (GRCm39) M944K possibly damaging Het
Afap1l2 T C 19: 56,906,427 (GRCm39) K491E probably damaging Het
Ahr A T 12: 35,561,164 (GRCm39) D150E probably damaging Het
Arhgef1 T A 7: 24,607,304 (GRCm39) L17Q probably damaging Het
Bysl A G 17: 47,915,242 (GRCm39) S169P Het
C4b A T 17: 34,948,404 (GRCm39) F1610I probably damaging Het
Chtf8 C A 8: 107,612,481 (GRCm39) A153S probably benign Het
Ciapin1 C T 8: 95,558,400 (GRCm39) probably null Het
Cnnm1 A G 19: 43,429,395 (GRCm39) E171G possibly damaging Het
Crybb3 T C 5: 113,225,407 (GRCm39) N155S probably benign Het
Dclk3 T A 9: 111,317,683 (GRCm39) M773K probably benign Het
Ddx18 A T 1: 121,489,267 (GRCm39) V260D probably damaging Het
Dmxl2 T A 9: 54,307,321 (GRCm39) N2216Y Het
Epn2 T C 11: 61,437,446 (GRCm39) E42G probably damaging Het
Esrrg C A 1: 187,878,605 (GRCm39) P229T probably benign Het
Esrrg C A 1: 187,878,606 (GRCm39) P229Q possibly damaging Het
Ethe1 G T 7: 24,305,643 (GRCm39) R130L probably benign Het
Fgl2 G A 5: 21,580,363 (GRCm39) C235Y probably damaging Het
Fmnl1 C T 11: 103,087,327 (GRCm39) T890M unknown Het
Fsip2 C G 2: 82,807,301 (GRCm39) P1207A probably benign Het
Gc C T 5: 89,593,165 (GRCm39) D85N possibly damaging Het
Gfi1 G A 5: 107,869,370 (GRCm39) R287W probably damaging Het
Gm3629 T C 14: 17,834,566 (GRCm39) probably null Het
Hapln3 A G 7: 78,771,712 (GRCm39) F59S probably benign Het
Il24 G A 1: 130,813,483 (GRCm39) T38I possibly damaging Het
Izumo1 A G 7: 45,276,583 (GRCm39) D382G probably benign Het
Kdm5a T A 6: 120,382,887 (GRCm39) Y739* probably null Het
Kif2b T A 11: 91,466,538 (GRCm39) I582F possibly damaging Het
Klhdc1 A G 12: 69,288,783 (GRCm39) Y31C probably damaging Het
Lama1 G A 17: 68,128,669 (GRCm39) E3009K Het
Mad2l1bp G A 17: 46,458,958 (GRCm39) R191C probably damaging Het
Mark1 A C 1: 184,644,261 (GRCm39) S468A probably damaging Het
Muc2 T A 7: 141,287,983 (GRCm39) C251* probably null Het
Nacad T C 11: 6,552,252 (GRCm39) D313G probably damaging Het
Neb G T 2: 52,099,599 (GRCm39) D4750E probably benign Het
Nlrp6 T C 7: 140,506,332 (GRCm39) S758P probably damaging Het
Nxt2 C T X: 141,020,747 (GRCm39) A118V possibly damaging Het
Or13a28 A C 7: 140,218,282 (GRCm39) I223L probably benign Het
Or51b4 T C 7: 103,530,569 (GRCm39) T294A possibly damaging Het
Or8b3b A G 9: 38,583,892 (GRCm39) F283L probably benign Het
Oxtr A G 6: 112,466,481 (GRCm39) V93A probably damaging Het
Pbx3 C A 2: 34,103,271 (GRCm39) D234Y probably damaging Het
Pde4b A T 4: 102,459,826 (GRCm39) T554S probably damaging Het
Peg10 GC GCTCC 6: 4,756,452 (GRCm39) probably benign Het
Pid1 A T 1: 84,015,955 (GRCm39) Y151N probably damaging Het
Plcd4 A G 1: 74,591,307 (GRCm39) E234G possibly damaging Het
Pou6f1 A G 15: 100,478,789 (GRCm39) V373A probably benign Het
Ppp1r12c T C 7: 4,485,792 (GRCm39) K685E possibly damaging Het
Rnf34 T A 5: 122,999,957 (GRCm39) V71D probably damaging Het
Rpap2 T G 5: 107,746,156 (GRCm39) H11Q possibly damaging Het
Skint2 A G 4: 112,496,834 (GRCm39) T247A probably benign Het
Stat5b T A 11: 100,692,350 (GRCm39) N145Y probably damaging Het
Sv2b A G 7: 74,856,017 (GRCm39) L91P probably damaging Het
Syt1 A G 10: 108,340,376 (GRCm39) I314T probably damaging Het
Tlk1 A T 2: 70,572,502 (GRCm39) N355K probably benign Het
Tnc C A 4: 63,890,973 (GRCm39) M1636I probably benign Het
Trank1 C A 9: 111,162,843 (GRCm39) A34D probably damaging Het
Unc13c A G 9: 73,719,665 (GRCm39) I1001T probably benign Het
Vdr T C 15: 97,765,136 (GRCm39) D201G probably benign Het
Vmn2r115 A T 17: 23,564,307 (GRCm39) I160L probably benign Het
Wdr81 C A 11: 75,335,250 (GRCm39) R1772L probably damaging Het
Zfp457 T C 13: 67,442,068 (GRCm39) D169G probably benign Het
Other mutations in Mknk2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01468:Mknk2 APN 10 80,503,498 (GRCm39) splice site probably benign
IGL02471:Mknk2 APN 10 80,503,955 (GRCm39) missense probably damaging 0.99
IGL02643:Mknk2 APN 10 80,504,435 (GRCm39) missense probably damaging 1.00
H8562:Mknk2 UTSW 10 80,504,768 (GRCm39) splice site probably benign
IGL03052:Mknk2 UTSW 10 80,505,496 (GRCm39) missense probably benign 0.12
R0645:Mknk2 UTSW 10 80,507,742 (GRCm39) splice site probably null
R2061:Mknk2 UTSW 10 80,507,391 (GRCm39) critical splice donor site probably null
R2105:Mknk2 UTSW 10 80,504,435 (GRCm39) missense possibly damaging 0.90
R2167:Mknk2 UTSW 10 80,504,535 (GRCm39) missense probably damaging 1.00
R3847:Mknk2 UTSW 10 80,503,809 (GRCm39) nonsense probably null
R4649:Mknk2 UTSW 10 80,505,173 (GRCm39) missense probably damaging 1.00
R5062:Mknk2 UTSW 10 80,507,603 (GRCm39) missense probably damaging 1.00
R5358:Mknk2 UTSW 10 80,507,597 (GRCm39) missense probably benign 0.19
R5433:Mknk2 UTSW 10 80,503,059 (GRCm39) missense probably benign 0.00
R5518:Mknk2 UTSW 10 80,504,475 (GRCm39) missense possibly damaging 0.92
R5813:Mknk2 UTSW 10 80,511,696 (GRCm39) missense probably benign 0.34
R6060:Mknk2 UTSW 10 80,507,468 (GRCm39) missense probably benign 0.00
R6151:Mknk2 UTSW 10 80,504,859 (GRCm39) splice site probably null
R6366:Mknk2 UTSW 10 80,507,767 (GRCm39) missense probably damaging 0.99
R7640:Mknk2 UTSW 10 80,504,400 (GRCm39) missense probably benign 0.00
R7827:Mknk2 UTSW 10 80,503,021 (GRCm39) missense probably benign 0.03
R7943:Mknk2 UTSW 10 80,511,701 (GRCm39) missense probably benign 0.00
R8075:Mknk2 UTSW 10 80,507,982 (GRCm39) intron probably benign
R9140:Mknk2 UTSW 10 80,507,427 (GRCm39) missense probably benign 0.22
R9451:Mknk2 UTSW 10 80,505,496 (GRCm39) missense probably benign 0.12
R9506:Mknk2 UTSW 10 80,503,918 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TCAAAGGCTACCGATGCATC -3'
(R):5'- TCCTATGTGAGCACCCCAAC -3'

Sequencing Primer
(F):5'- CAGGTGACACGAGCTGACTAC -3'
(R):5'- AACCAGGTGAGGCTGCCTG -3'
Posted On 2021-12-30