Mutagenetix > Incidental Mutation

Incidental Mutation 'R8928:Or5k15'

692992

Institutional Source

Beutler Lab

Gene Symbol

Or5k15

Ensembl Gene

ENSMUSG00000044029

Gene Name

olfactory receptor family 5 subfamily J member 15

Synonyms

MOR184-6, Olfr178, GA_x54KRFPKG5P-55108059-55107100

MMRRC Submission

068772-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R8928 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

58709622-58710581 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 58709750 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 278 (T278S)

Ref Sequence

ENSEMBL: ENSMUSP00000145885 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058564] [ENSMUST00000206523] [ENSMUST00000215032]

AlphaFold

E9Q9T3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000058564
AA Change: T278S

PolyPhen 2 Score 0.753 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000049578
Gene: ENSMUSG00000044029
AA Change: T278S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	306	1.2e-49	PFAM
Pfam:7TM_GPCR_Srsx	37	262	4.4e-7	PFAM
Pfam:7tm_1	43	312	5.2e-19	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000206523
AA Change: T278S

PolyPhen 2 Score 0.753 (Sensitivity: 0.85; Specificity: 0.92)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215032
AA Change: T278S

PolyPhen 2 Score 0.753 (Sensitivity: 0.85; Specificity: 0.92)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahctf1	C	A	1: 179,596,626 (GRCm39)	S1056I	possibly damaging	Het
Ajm1	A	G	2: 25,468,577 (GRCm39)	Y445H	possibly damaging	Het
Akap13	T	A	7: 75,259,606 (GRCm39)	D743E	probably benign	Het
Alas1	T	A	9: 106,118,513 (GRCm39)	H203L	probably benign	Het
Ankfn1	G	A	11: 89,429,279 (GRCm39)	T35I	possibly damaging	Het
Ankrd11	C	T	8: 123,622,718 (GRCm39)	R378K	probably damaging	Het
Arap1	G	A	7: 101,057,324 (GRCm39)	R1355Q	possibly damaging	Het
Cabp7	T	A	11: 4,696,640 (GRCm39)	I30F	possibly damaging	Het
Ccdc113	T	C	8: 96,267,584 (GRCm39)		probably null	Het
Ccdc162	T	A	10: 41,462,245 (GRCm39)		probably benign	Het
Cdh1	ACTCGAAATGATGTGGCTC	ACTC	8: 107,392,870 (GRCm39)		probably benign	Het
Cep85	G	T	4: 133,859,715 (GRCm39)	H651Q	probably benign	Het
Cfap43	T	A	19: 47,804,399 (GRCm39)	I266L	probably benign	Het
Crybg3	T	C	16: 59,315,123 (GRCm39)	T1179A	probably benign	Het
Crybg3	A	G	16: 59,376,715 (GRCm39)	V1513A	probably benign	Het
Csmd2	G	T	4: 128,369,582 (GRCm39)	V1905L		Het
D630003M21Rik	T	C	2: 158,059,447 (GRCm39)	E151G	probably damaging	Het
Dnah10	A	T	5: 124,866,828 (GRCm39)	Y2306F	probably damaging	Het
Dpy19l4	A	T	4: 11,304,674 (GRCm39)		probably benign	Het
Dsc1	G	T	18: 20,243,225 (GRCm39)	T76K	probably benign	Het
Egfem1	A	C	3: 29,744,561 (GRCm39)	*591C	probably null	Het
Elapor2	T	A	5: 9,496,979 (GRCm39)	H814Q	possibly damaging	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Fn1	T	C	1: 71,641,777 (GRCm39)		probably benign	Het
Fsd2	T	C	7: 81,209,354 (GRCm39)	T163A	probably benign	Het
Gm45844	A	G	7: 7,281,094 (GRCm39)		probably benign	Het
Grid1	T	A	14: 35,302,723 (GRCm39)	V996D	probably benign	Het
Hepacam2	C	A	6: 3,467,623 (GRCm39)		probably null	Het
Hira	A	G	16: 18,716,537 (GRCm39)	T101A	probably benign	Het
Ighv8-9	T	A	12: 115,432,204 (GRCm39)	T36S	possibly damaging	Het
Lama5	A	T	2: 179,843,832 (GRCm39)	C413S	probably damaging	Het
Lpcat2b	G	T	5: 107,580,913 (GRCm39)	V81L	probably benign	Het
Map1b	C	G	13: 99,568,624 (GRCm39)	E1366Q	unknown	Het
Marchf4	C	A	1: 72,574,035 (GRCm39)	A88S	probably benign	Het
Mbnl2	A	G	14: 120,633,974 (GRCm39)	T324A	probably benign	Het
Mipol1	T	A	12: 57,507,651 (GRCm39)	M387K	probably benign	Het
Mtor	T	C	4: 148,623,356 (GRCm39)	S2069P	probably damaging	Het
Mycbp2	T	A	14: 103,393,781 (GRCm39)	T2927S	probably benign	Het
Nfatc1	A	T	18: 80,741,180 (GRCm39)	N273K	possibly damaging	Het
Or10p22	T	C	10: 128,826,317 (GRCm39)	C179R	probably damaging	Het
Or2i1	T	A	17: 37,507,861 (GRCm39)	T258S	probably damaging	Het
Or5v1	A	T	17: 37,809,583 (GRCm39)	I14F	probably damaging	Het
Or7a36	A	G	10: 78,820,547 (GRCm39)	K308E		Het
Or8b49	A	T	9: 38,505,662 (GRCm39)	L48F	probably damaging	Het
Parp16	A	G	9: 65,133,396 (GRCm39)	D79G	probably benign	Het
Ppp4r3b	T	C	11: 29,144,598 (GRCm39)	V316A	probably benign	Het
Ptcd2	G	A	13: 99,476,505 (GRCm39)	T78I	probably damaging	Het
Rbpjl	A	G	2: 164,250,313 (GRCm39)	Y155C	probably damaging	Het
Slc5a7	G	T	17: 54,591,258 (GRCm39)	Q225K	possibly damaging	Het
Stom	T	C	2: 35,205,937 (GRCm39)	N264S	probably damaging	Het
Tbc1d2b	C	T	9: 90,108,190 (GRCm39)	E454K	probably damaging	Het
Tmem209	C	T	6: 30,489,365 (GRCm39)	C495Y	probably damaging	Het
Tnn	T	C	1: 159,953,099 (GRCm39)	D647G	probably damaging	Het
Vmn2r44	A	T	7: 8,381,101 (GRCm39)	V264E	probably damaging	Het
Wscd2	A	T	5: 113,715,401 (GRCm39)	I414F	possibly damaging	Het
Xkr5	A	G	8: 18,983,787 (GRCm39)	V585A	probably benign	Het
Zfp456	G	A	13: 67,514,603 (GRCm39)	R368C	probably benign	Het
Zscan18	G	A	7: 12,509,120 (GRCm39)	Q127*	probably null	Het

Other mutations in Or5k15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00953:Or5k15	APN	16	58,710,048 (GRCm39)	missense	probably damaging	0.97
IGL01964:Or5k15	APN	16	58,709,827 (GRCm39)	missense	probably damaging	0.97
IGL02122:Or5k15	APN	16	58,710,134 (GRCm39)	missense	probably benign
IGL02183:Or5k15	APN	16	58,710,184 (GRCm39)	missense	probably benign	0.00
IGL03143:Or5k15	APN	16	58,709,824 (GRCm39)	missense	probably damaging	1.00
R1566:Or5k15	UTSW	16	58,709,903 (GRCm39)	missense	probably damaging	1.00
R2324:Or5k15	UTSW	16	58,710,503 (GRCm39)	missense	probably benign
R2420:Or5k15	UTSW	16	58,710,328 (GRCm39)	missense	probably benign	0.00
R2421:Or5k15	UTSW	16	58,710,328 (GRCm39)	missense	probably benign	0.00
R2422:Or5k15	UTSW	16	58,710,328 (GRCm39)	missense	probably benign	0.00
R4256:Or5k15	UTSW	16	58,710,143 (GRCm39)	missense	probably benign	0.21
R4374:Or5k15	UTSW	16	58,710,242 (GRCm39)	missense	probably benign	0.13
R4502:Or5k15	UTSW	16	58,710,539 (GRCm39)	missense	probably benign	0.02
R4503:Or5k15	UTSW	16	58,710,539 (GRCm39)	missense	probably benign	0.02
R4662:Or5k15	UTSW	16	58,710,287 (GRCm39)	missense	probably damaging	1.00
R4967:Or5k15	UTSW	16	58,709,957 (GRCm39)	missense	possibly damaging	0.48
R5206:Or5k15	UTSW	16	58,710,381 (GRCm39)	missense	probably damaging	0.99
R5285:Or5k15	UTSW	16	58,710,471 (GRCm39)	nonsense	probably null
R5477:Or5k15	UTSW	16	58,710,107 (GRCm39)	missense	probably benign	0.10
R5554:Or5k15	UTSW	16	58,710,169 (GRCm39)	missense	possibly damaging	0.95
R5723:Or5k15	UTSW	16	58,709,976 (GRCm39)	nonsense	probably null
R5725:Or5k15	UTSW	16	58,710,250 (GRCm39)	missense	possibly damaging	0.64
R6853:Or5k15	UTSW	16	58,710,122 (GRCm39)	missense	probably damaging	0.99
R6853:Or5k15	UTSW	16	58,710,121 (GRCm39)	missense	possibly damaging	0.64
R7238:Or5k15	UTSW	16	58,710,252 (GRCm39)	missense	probably damaging	1.00
R7554:Or5k15	UTSW	16	58,709,769 (GRCm39)	missense	probably benign	0.27
R7577:Or5k15	UTSW	16	58,709,629 (GRCm39)	missense	probably benign	0.22
R7787:Or5k15	UTSW	16	58,709,953 (GRCm39)	missense	probably benign
R8008:Or5k15	UTSW	16	58,710,251 (GRCm39)	missense	probably benign	0.13
R8140:Or5k15	UTSW	16	58,709,948 (GRCm39)	missense	probably benign	0.10
R9082:Or5k15	UTSW	16	58,709,834 (GRCm39)	missense	probably damaging	1.00
R9285:Or5k15	UTSW	16	58,710,569 (GRCm39)	nonsense	probably null
R9414:Or5k15	UTSW	16	58,710,565 (GRCm39)	missense	probably benign	0.03
R9627:Or5k15	UTSW	16	58,709,771 (GRCm39)	missense	probably benign	0.01
R9745:Or5k15	UTSW	16	58,710,265 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCACAGTTGTTCAATTAACCAGAC -3'
(R):5'- AGACTCTCCTGTACAGACCC -3'

Sequencing Primer
(F):5'- TGGGAATTACAATAGCATACTGTTC -3'
(R):5'- CCTGTACAGACCCATTTATCAATG -3'

Posted On

2022-01-20