Mutagenetix > Incidental Mutation

Incidental Mutation 'R9193:Or2a20'

697802

Institutional Source

Beutler Lab

Gene Symbol

Or2a20

Ensembl Gene

ENSMUSG00000059411

Gene Name

olfactory receptor family 2 subfamily A member 2

Synonyms

Olfr434, GA_x6K02T2P3E9-4341246-4340281, MOR261-10

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R9193 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

43193849-43194814 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 43194086 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 80 (T80S)

Ref Sequence

ENSEMBL: ENSMUSP00000149678 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076752] [ENSMUST00000216562]

AlphaFold

Q8VF17

Predicted Effect

probably benign
Transcript: ENSMUST00000076752
AA Change: T80S

PolyPhen 2 Score 0.452 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000076039
Gene: ENSMUSG00000059411
AA Change: T80S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	7.7e-62	PFAM
Pfam:7tm_1	40	289	2.1e-29	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216562
AA Change: T80S

PolyPhen 2 Score 0.452 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ak1	A	T	2: 32,520,391 (GRCm39)	K79M	probably benign	Het
Amy1	T	C	3: 113,356,278 (GRCm39)	K293E	probably benign	Het
Bcl2l10	A	G	9: 75,255,333 (GRCm39)	Q50R	probably benign	Het
Bpifa6	G	A	2: 153,826,740 (GRCm39)	G35D	probably benign	Het
Cachd1	C	A	4: 100,634,339 (GRCm39)	P5Q	unknown	Het
Capn13	A	T	17: 73,652,191 (GRCm39)	I266N	probably damaging	Het
Chil3	A	G	3: 106,063,081 (GRCm39)	S170P	possibly damaging	Het
Ckap4	A	G	10: 84,363,350 (GRCm39)	I571T	probably damaging	Het
Col14a1	A	G	15: 55,242,964 (GRCm39)	M371V	unknown	Het
Dag1	A	G	9: 108,085,467 (GRCm39)	L558P	possibly damaging	Het
Edem3	G	A	1: 151,694,270 (GRCm39)	E868K	probably benign	Het
Eif3k	T	C	7: 28,673,624 (GRCm39)	Y150C	probably damaging	Het
Farp1	T	C	14: 121,518,281 (GRCm39)	I988T	probably benign	Het
Fat3	A	G	9: 15,910,248 (GRCm39)	M1918T	probably benign	Het
Gba2	A	G	4: 43,578,112 (GRCm39)	I79T	probably benign	Het
Gbp8	T	C	5: 105,179,169 (GRCm39)	D110G	probably damaging	Het
Gm57858	G	T	3: 36,089,428 (GRCm39)	N165K	probably benign	Het
H2-M2	T	A	17: 37,793,428 (GRCm39)	M193L	probably benign	Het
H2-T13	A	G	17: 36,391,956 (GRCm39)	V39A	possibly damaging	Het
Ighv7-1	T	A	12: 113,860,110 (GRCm39)	D94V	probably damaging	Het
Il2ra	T	C	2: 11,689,202 (GRCm39)	F244L	possibly damaging	Het
Jag1	A	T	2: 136,931,764 (GRCm39)	H602Q	probably null	Het
Kidins220	A	T	12: 25,036,966 (GRCm39)	I16F	possibly damaging	Het
Klb	A	G	5: 65,529,368 (GRCm39)	T299A	possibly damaging	Het
Lama4	A	G	10: 38,951,444 (GRCm39)	K1063R	probably benign	Het
Lrit2	A	G	14: 36,794,550 (GRCm39)	E538G	possibly damaging	Het
Lrrc7	A	T	3: 158,059,011 (GRCm39)	L32*	probably null	Het
Lrrtm3	A	T	10: 63,765,883 (GRCm39)	I568N	probably damaging	Het
Myom1	A	G	17: 71,343,295 (GRCm39)	D164G	probably damaging	Het
Naaladl2	T	C	3: 23,900,742 (GRCm39)	D696G	probably damaging	Het
Nalcn	A	T	14: 123,545,792 (GRCm39)	L1073*	probably null	Het
Nid2	T	C	14: 19,853,278 (GRCm39)	S1166P	probably damaging	Het
Nlrp4c	T	C	7: 6,095,621 (GRCm39)	V833A	probably benign	Het
Nyap2	T	C	1: 81,275,712 (GRCm39)	S619P	probably damaging	Het
Or10d5j	T	C	9: 39,867,878 (GRCm39)	M118V	probably damaging	Het
Or4a71	A	G	2: 89,357,987 (GRCm39)	Y256H	probably damaging	Het
Or8k16	C	A	2: 85,520,644 (GRCm39)	Y290*	probably null	Het
P3h2	T	C	16: 25,923,991 (GRCm39)	N148S	probably benign	Het
P4htm	A	G	9: 108,460,081 (GRCm39)	M231T	probably damaging	Het
Pank1	T	A	19: 34,804,634 (GRCm39)	K349I	possibly damaging	Het
Pgam5	A	G	5: 110,413,466 (GRCm39)	Y210H	probably benign	Het
Prb1b	T	C	6: 132,288,996 (GRCm39)	H276R	unknown	Het
Prl2a1	T	A	13: 27,992,535 (GRCm39)	C220S	probably damaging	Het
Psca	C	T	15: 74,587,932 (GRCm39)	Q39*	probably null	Het
Rgs20	A	T	1: 5,091,067 (GRCm39)	M121K	possibly damaging	Het
S100z	C	T	13: 95,613,883 (GRCm39)	V76M	possibly damaging	Het
Slc30a10	T	C	1: 185,195,034 (GRCm39)	I282T	probably damaging	Het
Slc30a3	T	C	5: 31,246,088 (GRCm39)	Y214C	probably damaging	Het
Sox5	C	T	6: 143,790,570 (GRCm39)	E497K	probably benign	Het
Spata31g1	A	G	4: 42,971,519 (GRCm39)	D284G	probably benign	Het
Sptbn1	C	T	11: 30,087,551 (GRCm39)	E963K	possibly damaging	Het
Srl	T	C	16: 4,311,723 (GRCm39)	E465G	possibly damaging	Het
Tacc2	T	C	7: 130,228,304 (GRCm39)	M1663T	probably benign	Het
Tas2r119	T	G	15: 32,177,932 (GRCm39)	V166G	probably benign	Het
Tcf7l1	A	G	6: 72,611,205 (GRCm39)	V191A	probably damaging	Het
Thap7	A	T	16: 17,346,901 (GRCm39)	I92K	probably damaging	Het
Tnfsf10	A	G	3: 27,371,407 (GRCm39)	T38A	possibly damaging	Het
Trav7-5	C	G	14: 53,768,615 (GRCm39)	A61G	probably benign	Het
Trim30c	C	T	7: 104,031,553 (GRCm39)	V421I	probably benign	Het
Ugt2b38	A	T	5: 87,571,729 (GRCm39)	M101K	probably benign	Het
Vmn1r44	T	A	6: 89,870,565 (GRCm39)	C104S	probably damaging	Het
Wdr74	T	A	19: 8,715,240 (GRCm39)	V133E	probably damaging	Het
Zfp735	A	T	11: 73,580,600 (GRCm39)	Y33F	possibly damaging	Het

Other mutations in Or2a20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00339:Or2a20	APN	6	43,194,782 (GRCm39)	missense	probably benign	0.00
R0347:Or2a20	UTSW	6	43,194,296 (GRCm39)	missense	probably benign
R1099:Or2a20	UTSW	6	43,194,558 (GRCm39)	missense	probably damaging	1.00
R1147:Or2a20	UTSW	6	43,194,146 (GRCm39)	missense	probably damaging	0.99
R1147:Or2a20	UTSW	6	43,194,146 (GRCm39)	missense	probably damaging	0.99
R1353:Or2a20	UTSW	6	43,194,624 (GRCm39)	missense	probably benign	0.41
R1570:Or2a20	UTSW	6	43,194,285 (GRCm39)	missense	probably benign
R1720:Or2a20	UTSW	6	43,194,494 (GRCm39)	missense	probably damaging	1.00
R2098:Or2a20	UTSW	6	43,194,437 (GRCm39)	missense	probably benign	0.02
R4707:Or2a20	UTSW	6	43,193,883 (GRCm39)	missense	probably benign	0.27
R4942:Or2a20	UTSW	6	43,193,928 (GRCm39)	missense	probably damaging	1.00
R5008:Or2a20	UTSW	6	43,193,991 (GRCm39)	missense	probably damaging	0.98
R5084:Or2a20	UTSW	6	43,194,594 (GRCm39)	missense	probably damaging	1.00
R6472:Or2a20	UTSW	6	43,194,293 (GRCm39)	missense	probably benign
R7592:Or2a20	UTSW	6	43,194,179 (GRCm39)	missense	probably damaging	1.00
R7756:Or2a20	UTSW	6	43,193,950 (GRCm39)	nonsense	probably null
R7784:Or2a20	UTSW	6	43,194,322 (GRCm39)	missense	possibly damaging	0.82
R8056:Or2a20	UTSW	6	43,193,978 (GRCm39)	missense	probably damaging	0.99
R9093:Or2a20	UTSW	6	43,194,500 (GRCm39)	missense	probably benign
R9745:Or2a20	UTSW	6	43,194,258 (GRCm39)	nonsense	probably null
R9749:Or2a20	UTSW	6	43,194,258 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACCATGGTCACAGAGTTCGTC -3'
(R):5'- CTCTCCAGTTCATGATGACAGTG -3'

Sequencing Primer
(F):5'- GTCACAGAGTTCGTCCTGCTG -3'
(R):5'- CTCCAGTTCATGATGACAGTGTAGTG -3'

Posted On

2022-02-07