Mutagenetix > Incidental Mutation

Incidental Mutation 'R1421:Zfp735'

159824

Institutional Source

Beutler Lab

Gene Symbol

Zfp735

Ensembl Gene

ENSMUSG00000060630

Gene Name

zinc finger protein 735

Synonyms

1700012C15Rik

MMRRC Submission

039477-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R1421 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73579604-73604624 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 73601523 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Isoleucine at position 156 (L156I)

Ref Sequence

ENSEMBL: ENSMUSP00000079269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080407]

AlphaFold

B1ARH2

Predicted Effect

probably benign
Transcript: ENSMUST00000080407
AA Change: L156I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000079269
Gene: ENSMUSG00000060630
AA Change: L156I

Domain	Start	End	E-Value	Type
KRAB	8	68	2.2e-34	SMART
ZnF_C2H2	483	505	4.38e1	SMART
ZnF_C2H2	511	533	2.67e-1	SMART
ZnF_C2H2	539	561	1.81e1	SMART
ZnF_C2H2	567	589	1.5e-4	SMART
ZnF_C2H2	595	617	4.87e-4	SMART
ZnF_C2H2	623	645	4.24e-4	SMART
ZnF_C2H2	651	673	2.27e-4	SMART
ZnF_C2H2	679	701	7.49e-5	SMART
ZnF_C2H2	707	729	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145996

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149560

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.7%

Validation Efficiency

96% (54/56)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	T	C	6: 128,520,923 (GRCm39)	T1344A	probably benign	Het
Abcf1	G	A	17: 36,271,801 (GRCm39)	A375V	probably damaging	Het
Adam20	T	A	8: 41,249,784 (GRCm39)	H631Q	possibly damaging	Het
Adcy10	T	C	1: 165,391,516 (GRCm39)	S1258P	probably damaging	Het
Agtpbp1	A	G	13: 59,643,389 (GRCm39)	I717T	possibly damaging	Het
Ahnak	T	A	19: 8,992,995 (GRCm39)	F4760I	possibly damaging	Het
Ano6	A	G	15: 95,811,266 (GRCm39)	K122R	probably benign	Het
Arhgap5	T	C	12: 52,563,631 (GRCm39)	C201R	probably damaging	Het
Atg16l1	T	C	1: 87,714,080 (GRCm39)		probably benign	Het
Cdhr3	A	T	12: 33,110,291 (GRCm39)	I331K	probably damaging	Het
Coq8a	A	G	1: 179,998,006 (GRCm39)		probably benign	Het
Crebbp	A	G	16: 3,942,511 (GRCm39)	V662A	probably damaging	Het
Cspg4	T	A	9: 56,803,910 (GRCm39)	M1667K	probably benign	Het
Dnah7a	A	G	1: 53,580,032 (GRCm39)		probably benign	Het
Dnajc6	A	T	4: 101,468,513 (GRCm39)	Y251F	probably damaging	Het
Dpy19l4	A	T	4: 11,304,011 (GRCm39)	M133K	probably benign	Het
Emb	T	C	13: 117,408,624 (GRCm39)	Y322H	probably benign	Het
Gcm1	A	T	9: 77,966,982 (GRCm39)	H67L	probably damaging	Het
Gls2	A	T	10: 128,037,217 (GRCm39)	K253*	probably null	Het
Gm28042	T	A	2: 119,866,944 (GRCm39)	S196T	probably benign	Het
Gm43302	T	C	5: 105,365,215 (GRCm39)	T598A	probably benign	Het
Gramd1a	T	A	7: 30,842,291 (GRCm39)	Q90L	probably damaging	Het
Grhl2	A	G	15: 37,309,960 (GRCm39)	Y352C	probably damaging	Het
Ifi203-ps	T	C	1: 173,625,563 (GRCm39)		noncoding transcript	Het
Ifitm2	T	C	7: 140,534,972 (GRCm39)	I121V	probably benign	Het
Insyn2a	A	T	7: 134,500,960 (GRCm39)		probably benign	Het
Kptn	T	G	7: 15,856,949 (GRCm39)		probably benign	Het
L2hgdh	C	T	12: 69,748,092 (GRCm39)	D345N	probably benign	Het
Lgals12	T	C	19: 7,584,079 (GRCm39)	H6R	probably benign	Het
Lrrc4b	A	G	7: 44,110,475 (GRCm39)	I116V	probably benign	Het
Misp	A	G	10: 79,662,681 (GRCm39)	D366G	probably damaging	Het
Nav1	T	C	1: 135,512,748 (GRCm39)	E104G	probably damaging	Het
Nup155	A	T	15: 8,187,244 (GRCm39)	H1391L	probably damaging	Het
Parp1	T	C	1: 180,427,653 (GRCm39)		probably benign	Het
Pikfyve	G	A	1: 65,310,470 (GRCm39)	G1919D	probably damaging	Het
Pomt1	A	G	2: 32,126,765 (GRCm39)		probably benign	Het
Prrc2b	C	T	2: 32,090,990 (GRCm39)	S454F	possibly damaging	Het
Selenbp1	T	C	3: 94,851,183 (GRCm39)	S360P	probably benign	Het
Slc6a12	T	A	6: 121,336,085 (GRCm39)	I352N	probably damaging	Het
Snx9	G	A	17: 5,952,759 (GRCm39)	G197D	probably benign	Het
Ston1	T	C	17: 88,943,221 (GRCm39)	V209A	probably benign	Het
Taf7l2	A	G	10: 115,949,343 (GRCm39)	V61A	probably damaging	Het
Tex36	A	T	7: 133,197,078 (GRCm39)		probably null	Het
Tnnt3	A	G	7: 142,065,103 (GRCm39)	E108G	probably damaging	Het
Vmn2r98	T	A	17: 19,285,440 (GRCm39)	F87I	probably damaging	Het
Vwa8	C	T	14: 79,145,670 (GRCm39)	R116C	probably damaging	Het
Wdr64	T	C	1: 175,594,716 (GRCm39)	I479T	possibly damaging	Het
Xrcc5	A	G	1: 72,349,636 (GRCm39)	N22D	probably benign	Het
Zfp407	C	T	18: 84,577,898 (GRCm39)	A1072T	probably benign	Het
Zfp820	A	T	17: 22,038,861 (GRCm39)	Y156N	possibly damaging	Het

Other mutations in Zfp735

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Zfp735	APN	11	73,602,192 (GRCm39)	missense	possibly damaging	0.86
IGL00798:Zfp735	APN	11	73,602,386 (GRCm39)	missense	possibly damaging	0.72
IGL01642:Zfp735	APN	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
IGL01684:Zfp735	APN	11	73,581,191 (GRCm39)	missense	possibly damaging	0.86
IGL02096:Zfp735	APN	11	73,602,254 (GRCm39)	missense	probably benign	0.01
IGL02238:Zfp735	APN	11	73,601,319 (GRCm39)	missense	probably benign	0.00
IGL02505:Zfp735	APN	11	73,580,626 (GRCm39)	missense	probably benign	0.03
IGL02740:Zfp735	APN	11	73,601,412 (GRCm39)	missense	possibly damaging	0.53
IGL02957:Zfp735	APN	11	73,601,755 (GRCm39)	missense	probably benign	0.00
bananaquit	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
bescher	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
Galvanic	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
grassquit	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R0114:Zfp735	UTSW	11	73,601,488 (GRCm39)	missense	probably benign	0.33
R0217:Zfp735	UTSW	11	73,602,112 (GRCm39)	missense	possibly damaging	0.73
R0943:Zfp735	UTSW	11	73,602,909 (GRCm39)	missense	probably benign	0.04
R1460:Zfp735	UTSW	11	73,603,159 (GRCm39)	missense	possibly damaging	0.73
R1493:Zfp735	UTSW	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
R1517:Zfp735	UTSW	11	73,601,470 (GRCm39)	missense	probably benign
R1676:Zfp735	UTSW	11	73,602,301 (GRCm39)	missense	possibly damaging	0.53
R1709:Zfp735	UTSW	11	73,602,589 (GRCm39)	missense	probably benign	0.01
R1871:Zfp735	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
R1931:Zfp735	UTSW	11	73,602,677 (GRCm39)	missense	possibly damaging	0.69
R2219:Zfp735	UTSW	11	73,601,851 (GRCm39)	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73,602,223 (GRCm39)	nonsense	probably null
R2227:Zfp735	UTSW	11	73,602,222 (GRCm39)	missense	possibly damaging	0.53
R3552:Zfp735	UTSW	11	73,602,067 (GRCm39)	nonsense	probably null
R3856:Zfp735	UTSW	11	73,602,282 (GRCm39)	missense	probably benign	0.01
R3925:Zfp735	UTSW	11	73,601,950 (GRCm39)	missense	probably benign	0.33
R4572:Zfp735	UTSW	11	73,580,611 (GRCm39)	missense	probably benign	0.02
R4585:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4586:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4619:Zfp735	UTSW	11	73,602,031 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,682 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,681 (GRCm39)	missense	probably damaging	0.98
R5435:Zfp735	UTSW	11	73,602,939 (GRCm39)	missense	possibly damaging	0.72
R5489:Zfp735	UTSW	11	73,601,419 (GRCm39)	nonsense	probably null
R5516:Zfp735	UTSW	11	73,601,640 (GRCm39)	missense	probably benign
R5654:Zfp735	UTSW	11	73,602,964 (GRCm39)	missense	possibly damaging	0.71
R5990:Zfp735	UTSW	11	73,581,174 (GRCm39)	missense	possibly damaging	0.70
R6332:Zfp735	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
R6427:Zfp735	UTSW	11	73,581,140 (GRCm39)	missense	possibly damaging	0.73
R6460:Zfp735	UTSW	11	73,602,478 (GRCm39)	missense	probably benign	0.33
R6820:Zfp735	UTSW	11	73,579,783 (GRCm39)	start codon destroyed	probably null	0.01
R6831:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6833:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6834:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6897:Zfp735	UTSW	11	73,601,880 (GRCm39)	missense	probably benign	0.08
R6941:Zfp735	UTSW	11	73,581,159 (GRCm39)	missense	probably benign	0.33
R7335:Zfp735	UTSW	11	73,602,379 (GRCm39)	missense	possibly damaging	0.47
R7366:Zfp735	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
R7474:Zfp735	UTSW	11	73,602,002 (GRCm39)	missense	possibly damaging	0.72
R7487:Zfp735	UTSW	11	73,581,154 (GRCm39)	missense	possibly damaging	0.53
R7583:Zfp735	UTSW	11	73,601,933 (GRCm39)	missense	possibly damaging	0.86
R7866:Zfp735	UTSW	11	73,601,629 (GRCm39)	missense	probably benign	0.00
R8005:Zfp735	UTSW	11	73,603,140 (GRCm39)	nonsense	probably null
R8500:Zfp735	UTSW	11	73,601,811 (GRCm39)	missense	possibly damaging	0.53
R8551:Zfp735	UTSW	11	73,603,122 (GRCm39)	missense	probably benign	0.06
R8754:Zfp735	UTSW	11	73,603,000 (GRCm39)	missense	possibly damaging	0.85
R8769:Zfp735	UTSW	11	73,581,127 (GRCm39)	missense	possibly damaging	0.53
R8794:Zfp735	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R8835:Zfp735	UTSW	11	73,601,692 (GRCm39)	missense	possibly damaging	0.53
R8869:Zfp735	UTSW	11	73,602,510 (GRCm39)	missense	possibly damaging	0.53
R8969:Zfp735	UTSW	11	73,602,699 (GRCm39)	missense	possibly damaging	0.83
R9072:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9073:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9193:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	possibly damaging	0.71
R9355:Zfp735	UTSW	11	73,602,362 (GRCm39)	missense	probably benign	0.01
R9414:Zfp735	UTSW	11	73,602,023 (GRCm39)	nonsense	probably null
R9456:Zfp735	UTSW	11	73,602,403 (GRCm39)	missense	possibly damaging	0.53
R9573:Zfp735	UTSW	11	73,602,936 (GRCm39)	missense	possibly damaging	0.67
R9647:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	probably damaging	0.98
R9710:Zfp735	UTSW	11	73,601,806 (GRCm39)	missense	possibly damaging	0.86
Z1176:Zfp735	UTSW	11	73,601,641 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TCTTGGAACAAAGTGATGCACTTCTGT -3'
(R):5'- CCCACAACTGCTTCCCATGTGA -3'

Sequencing Primer
(F):5'- CCAAGAAGCTATTACCAAAGGTGTG -3'
(R):5'- GCTTCCCATGTGATATATTCCAAG -3'

Posted On

2014-03-14