Mutagenetix > Incidental Mutation

Incidental Mutation 'R4026:Gm5581'

312984

Institutional Source

Beutler Lab

Gene Symbol

Gm5581

Ensembl Gene

ENSMUSG00000061969

Gene Name

predicted gene 5581

Synonyms

MMRRC Submission

040849-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R4026 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

131143329-131144284 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to A at 131144031 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000072940

SMART Domains

Protein: ENSMUSP00000072710
Gene: ENSMUSG00000061969

Domain	Start	End	E-Value	Type
KRAB	8	68	1.79e-34	SMART
low complexity region	285	302	N/A	INTRINSIC
low complexity region	355	366	N/A	INTRINSIC
PDB:1MEY\|G	422	507	4e-15	PDB
Blast:HNHc	445	504	4e-6	BLAST
ZnF_C2H2	509	531	9.73e-4	SMART
ZnF_C2H2	537	559	1.5e-4	SMART
ZnF_C2H2	565	587	1.98e-4	SMART
ZnF_C2H2	593	615	1.52e-5	SMART
ZnF_C2H2	621	643	9.73e-4	SMART
ZnF_C2H2	649	671	9.36e-6	SMART
ZnF_C2H2	677	699	3.49e-5	SMART
ZnF_C2H2	705	727	9.88e-5	SMART
ZnF_C2H2	733	755	9.88e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204992

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.1%

Validation Efficiency

97% (36/37)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg7	A	T	16: 56,550,661 (GRCm39)	Y684N	probably damaging	Het
Ahnak	G	T	19: 8,988,663 (GRCm39)	V3316F	probably damaging	Het
Cacna1a	C	T	8: 85,307,962 (GRCm39)	T1409I	probably damaging	Het
Ccdc158	A	G	5: 92,791,666 (GRCm39)	M698T	probably benign	Het
Ces2g	T	A	8: 105,691,377 (GRCm39)	V171E	probably damaging	Het
Ctnna2	A	T	6: 77,613,827 (GRCm39)	D254E	possibly damaging	Het
Dlec1	T	C	9: 118,966,408 (GRCm39)	Y1126H	probably damaging	Het
Dspp	G	A	5: 104,325,563 (GRCm39)	S642N	unknown	Het
Fezf2	A	T	14: 12,343,986 (GRCm38)	C302S	probably damaging	Het
Gm10754	A	G	10: 97,517,978 (GRCm39)		probably benign	Het
Gm7929	T	C	14: 41,472,509 (GRCm39)	K21E	probably damaging	Het
Hmcn1	A	T	1: 150,598,120 (GRCm39)	D1727E	probably benign	Het
Lrriq4	T	C	3: 30,704,422 (GRCm39)	V150A	possibly damaging	Het
Micu3	A	G	8: 40,812,496 (GRCm39)		probably benign	Het
Mrpl3	T	C	9: 104,948,685 (GRCm39)		probably null	Het
Myo1e	A	G	9: 70,232,157 (GRCm39)	I229V	probably benign	Het
Ncam1	T	C	9: 49,476,295 (GRCm39)	I265V	probably benign	Het
Ncoa7	T	C	10: 30,598,720 (GRCm39)	T68A	probably benign	Het
Oprk1	T	C	1: 5,668,908 (GRCm39)	V118A	probably benign	Het
Ppp3r1	T	C	11: 17,144,786 (GRCm39)	V133A	probably damaging	Het
Sidt1	A	G	16: 44,102,249 (GRCm39)	S304P	possibly damaging	Het
Srsf6	T	A	2: 162,776,211 (GRCm39)		probably benign	Het
Tas2r105	A	G	6: 131,663,789 (GRCm39)	V213A	probably benign	Het
Tlr12	C	T	4: 128,510,301 (GRCm39)	E650K	probably benign	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Trav4-2	T	A	14: 53,656,287 (GRCm39)	D96E	possibly damaging	Het
Vmn1r34	T	A	6: 66,614,688 (GRCm39)	M17L	probably benign	Het
Vmn2r6	A	T	3: 64,445,671 (GRCm39)	S685T	possibly damaging	Het
Vmn2r89	T	C	14: 51,689,500 (GRCm39)	M1T	probably null	Het
Wdr20	A	G	12: 110,759,950 (GRCm39)	T279A	probably benign	Het
Zfp407	A	G	18: 84,577,721 (GRCm39)	S1131P	possibly damaging	Het
Zfyve1	A	G	12: 83,641,296 (GRCm39)	V120A	probably benign	Het

Other mutations in Gm5581

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00533:Gm5581	APN	6	131,144,604 (GRCm39)	unclassified	noncoding transcript
IGL01372:Gm5581	APN	6	131,145,366 (GRCm39)	exon	noncoding transcript
IGL01630:Gm5581	APN	6	131,145,259 (GRCm39)	exon	noncoding transcript
IGL01667:Gm5581	APN	6	131,144,735 (GRCm39)	unclassified	noncoding transcript
IGL02260:Gm5581	APN	6	131,144,909 (GRCm39)	unclassified	noncoding transcript
IGL03212:Gm5581	APN	6	131,158,413 (GRCm39)	exon	noncoding transcript
IGL03306:Gm5581	APN	6	131,145,044 (GRCm39)	unclassified	noncoding transcript
R0366:Gm5581	UTSW	6	131,143,410 (GRCm39)	unclassified	noncoding transcript
R1764:Gm5581	UTSW	6	131,158,362 (GRCm39)	exon	noncoding transcript
R1961:Gm5581	UTSW	6	131,145,125 (GRCm39)	unclassified	noncoding transcript
R2129:Gm5581	UTSW	6	131,145,247 (GRCm39)	exon	noncoding transcript
R3177:Gm5581	UTSW	6	131,143,928 (GRCm39)	unclassified	noncoding transcript
R4289:Gm5581	UTSW	6	131,144,519 (GRCm39)	unclassified	noncoding transcript
R4943:Gm5581	UTSW	6	131,144,088 (GRCm39)	unclassified	noncoding transcript
R4961:Gm5581	UTSW	6	131,144,190 (GRCm39)	unclassified	noncoding transcript
R5817:Gm5581	UTSW	6	131,144,132 (GRCm39)	unclassified	noncoding transcript
R5944:Gm5581	UTSW	6	131,145,363 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- AAGGTTTCTCTCCTGTGTGAATTC -3'
(R):5'- TATCCAGTCCTCAAATCTTCAAGC -3'

Sequencing Primer
(F):5'- CTCCTGTGTGAATTCTGTAGTGAAC -3'
(R):5'- TTTGCCATGAAAACTGTACAGGAG -3'

Posted On

2015-04-30