Mutagenetix > Incidental Mutation

Incidental Mutation 'R4649:Mapk8ip3'

350640

Institutional Source

Beutler Lab

Gene Symbol

Mapk8ip3

Ensembl Gene

ENSMUSG00000024163

Gene Name

mitogen-activated protein kinase 8 interacting protein 3

Synonyms

JSAP1c, JSAP1b, JNK-interacting protein 3, Jip3, D17Wsu15e, JSAP1, JSAP1d, Syd2, sunday driver 2, JSAP1a, JUN/SAPK-associated protein 1, c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1

MMRRC Submission

041910-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.843) question?

Stock #

R4649 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25116480-25155951 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 25123726 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 599 (I599F)

Ref Sequence

ENSEMBL: ENSMUSP00000136924 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088345] [ENSMUST00000115228] [ENSMUST00000115229] [ENSMUST00000117509] [ENSMUST00000119115] [ENSMUST00000120035] [ENSMUST00000121723] [ENSMUST00000121787] [ENSMUST00000178969] [ENSMUST00000146923] [ENSMUST00000146706]

AlphaFold

Q9ESN9

Predicted Effect

probably damaging
Transcript: ENSMUST00000088345
AA Change: I599F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000085683
Gene: ENSMUSG00000024163
AA Change: I599F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.4e-72	PFAM
low complexity region	236	249	N/A	INTRINSIC
low complexity region	261	270	N/A	INTRINSIC
PDB:2W83\|D	417	472	6e-20	PDB
coiled coil region	525	555	N/A	INTRINSIC
low complexity region	582	596	N/A	INTRINSIC
low complexity region	754	769	N/A	INTRINSIC
low complexity region	893	901	N/A	INTRINSIC
low complexity region	928	940	N/A	INTRINSIC
SCOP:d1flga_	987	1167	4e-8	SMART
Blast:WD40	1075	1116	6e-18	BLAST
low complexity region	1260	1276	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115228
AA Change: I584F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110883
Gene: ENSMUSG00000024163
AA Change: I584F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.4e-72	PFAM
low complexity region	236	249	N/A	INTRINSIC
low complexity region	261	270	N/A	INTRINSIC
PDB:2W83\|D	411	466	7e-20	PDB
low complexity region	567	581	N/A	INTRINSIC
low complexity region	739	754	N/A	INTRINSIC
low complexity region	878	886	N/A	INTRINSIC
low complexity region	913	925	N/A	INTRINSIC
SCOP:d1flga_	972	1152	3e-8	SMART
Blast:WD40	1060	1101	6e-18	BLAST
low complexity region	1245	1261	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115229
AA Change: I607F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000110884
Gene: ENSMUSG00000024163
AA Change: I607F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	184	2.9e-60	PFAM
low complexity region	244	257	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:JIP_LZII	423	493	3.1e-32	PFAM
coiled coil region	533	563	N/A	INTRINSIC
low complexity region	590	604	N/A	INTRINSIC
low complexity region	762	777	N/A	INTRINSIC
low complexity region	901	909	N/A	INTRINSIC
low complexity region	936	948	N/A	INTRINSIC
SCOP:d1flga_	995	1175	4e-8	SMART
Blast:WD40	1083	1124	7e-18	BLAST
low complexity region	1268	1284	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000117509
AA Change: I576F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112712
Gene: ENSMUSG00000024163
AA Change: I576F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.4e-72	PFAM
low complexity region	238	247	N/A	INTRINSIC
PDB:2W83\|D	394	449	7e-20	PDB
coiled coil region	502	532	N/A	INTRINSIC
low complexity region	559	573	N/A	INTRINSIC
low complexity region	731	746	N/A	INTRINSIC
low complexity region	870	878	N/A	INTRINSIC
low complexity region	905	917	N/A	INTRINSIC
SCOP:d1flga_	964	1144	3e-8	SMART
Blast:WD40	1052	1093	6e-18	BLAST
low complexity region	1237	1253	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000119115
AA Change: I567F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112955
Gene: ENSMUSG00000024163
AA Change: I567F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.3e-72	PFAM
low complexity region	229	238	N/A	INTRINSIC
PDB:2W83\|D	385	440	7e-20	PDB
coiled coil region	493	523	N/A	INTRINSIC
low complexity region	550	564	N/A	INTRINSIC
low complexity region	722	737	N/A	INTRINSIC
low complexity region	861	869	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
SCOP:d1flga_	955	1135	3e-8	SMART
Blast:WD40	1043	1084	5e-18	BLAST
low complexity region	1228	1244	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000120035
AA Change: I598F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000114084
Gene: ENSMUSG00000024163
AA Change: I598F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.4e-72	PFAM
low complexity region	235	248	N/A	INTRINSIC
low complexity region	260	269	N/A	INTRINSIC
PDB:2W83\|D	416	471	6e-20	PDB
coiled coil region	524	554	N/A	INTRINSIC
low complexity region	581	595	N/A	INTRINSIC
low complexity region	753	768	N/A	INTRINSIC
low complexity region	892	900	N/A	INTRINSIC
low complexity region	927	939	N/A	INTRINSIC
SCOP:d1flga_	986	1166	3e-8	SMART
Blast:WD40	1074	1115	6e-18	BLAST
low complexity region	1259	1275	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000121723
AA Change: I568F

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000113698
Gene: ENSMUSG00000024163
AA Change: I568F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1e-72	PFAM
low complexity region	230	239	N/A	INTRINSIC
PDB:2W83\|D	386	441	7e-20	PDB
coiled coil region	494	524	N/A	INTRINSIC
low complexity region	551	565	N/A	INTRINSIC
low complexity region	723	738	N/A	INTRINSIC
low complexity region	862	870	N/A	INTRINSIC
low complexity region	897	909	N/A	INTRINSIC
SCOP:d1flga_	956	1136	3e-8	SMART
Blast:WD40	1044	1085	5e-18	BLAST
low complexity region	1229	1245	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132258

Predicted Effect

possibly damaging
Transcript: ENSMUST00000121787
AA Change: I562F

PolyPhen 2 Score 0.950 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000113753
Gene: ENSMUSG00000024163
AA Change: I562F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	3.8e-73	PFAM
low complexity region	230	239	N/A	INTRINSIC
PDB:2W83\|D	380	435	8e-20	PDB
coiled coil region	488	518	N/A	INTRINSIC
low complexity region	545	559	N/A	INTRINSIC
low complexity region	717	732	N/A	INTRINSIC
low complexity region	856	864	N/A	INTRINSIC
low complexity region	891	903	N/A	INTRINSIC
SCOP:d1flga_	950	1130	3e-8	SMART
Blast:WD40	1038	1079	6e-18	BLAST
low complexity region	1223	1239	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000178969
AA Change: I599F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000136924
Gene: ENSMUSG00000024163
AA Change: I599F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.1e-72	PFAM
low complexity region	236	249	N/A	INTRINSIC
low complexity region	261	270	N/A	INTRINSIC
PDB:2W83\|D	417	472	6e-20	PDB
coiled coil region	525	555	N/A	INTRINSIC
low complexity region	582	596	N/A	INTRINSIC
low complexity region	754	769	N/A	INTRINSIC
low complexity region	893	901	N/A	INTRINSIC
low complexity region	928	940	N/A	INTRINSIC
SCOP:d1flga_	987	1167	3e-8	SMART
Blast:WD40	1075	1116	6e-18	BLAST
low complexity region	1260	1276	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000146923
AA Change: I599F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000114802
Gene: ENSMUSG00000024163
AA Change: I599F

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	29	186	1.4e-72	PFAM
low complexity region	236	249	N/A	INTRINSIC
low complexity region	261	270	N/A	INTRINSIC
PDB:2W83\|D	417	472	6e-20	PDB
coiled coil region	525	555	N/A	INTRINSIC
low complexity region	582	596	N/A	INTRINSIC
low complexity region	754	769	N/A	INTRINSIC
low complexity region	893	901	N/A	INTRINSIC
low complexity region	928	940	N/A	INTRINSIC
SCOP:d1flga_	987	1167	4e-8	SMART
Blast:WD40	1075	1116	6e-18	BLAST
low complexity region	1260	1276	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125648

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156663

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152486

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144336

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133198

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145357

Predicted Effect

probably benign
Transcript: ENSMUST00000146706

SMART Domains

Protein: ENSMUSP00000118422
Gene: ENSMUSG00000024163

Domain	Start	End	E-Value	Type
low complexity region	55	64	N/A	INTRINSIC
Pfam:JIP_LZII	203	235	1.6e-17	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous inactivation of this gene results in impaired lung morphogenesis, causes absence of the telencephalic commissure with multiple defects in brain development and axon guidance, may affect synaptic transmission, and invariably leads to neonatal death due to respiratory failure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
4933409G03Rik	G	A	2: 68,436,559 (GRCm39)	E168K	unknown	Het
Abca4	G	T	3: 121,963,542 (GRCm39)	V841F	probably damaging	Het
Adcy10	G	A	1: 165,331,618 (GRCm39)	E84K	probably damaging	Het
Adgrg6	C	A	10: 14,344,571 (GRCm39)	M127I	probably damaging	Het
Agpat5	A	G	8: 18,929,668 (GRCm39)	I266V	possibly damaging	Het
Agtpbp1	A	T	13: 59,676,213 (GRCm39)	Y186N	possibly damaging	Het
Alx1	A	T	10: 102,845,193 (GRCm39)	N279K	probably damaging	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Arsi	A	G	18: 61,050,170 (GRCm39)	Y351C	probably damaging	Het
B4galnt3	T	A	6: 120,201,581 (GRCm39)	R155W	probably damaging	Het
BC028528	T	C	3: 95,795,577 (GRCm39)	E75G	probably damaging	Het
Bmp2k	C	T	5: 97,200,970 (GRCm39)	P297S	possibly damaging	Het
Btnl4	A	G	17: 34,691,602 (GRCm39)	S296P	probably benign	Het
Bzw2	A	T	12: 36,173,980 (GRCm39)	V98E	probably null	Het
Cacna1d	C	T	14: 29,817,365 (GRCm39)	M1232I	probably benign	Het
Cast	A	G	13: 74,894,133 (GRCm39)	S171P	probably benign	Het
Catsperb	G	A	12: 101,507,771 (GRCm39)	A513T	probably benign	Het
Cd19	A	G	7: 126,013,664 (GRCm39)	S42P	probably benign	Het
Chml	A	G	1: 175,514,962 (GRCm39)	S138P	probably benign	Het
Clic6	T	G	16: 92,327,827 (GRCm39)		probably null	Het
Col12a1	A	T	9: 79,547,076 (GRCm39)	V2252E	probably damaging	Het
Cplx2	G	A	13: 54,527,361 (GRCm39)	E82K	probably benign	Het
Csmd2	G	A	4: 128,439,866 (GRCm39)	V3107M	probably benign	Het
Cspg4	T	A	9: 56,794,149 (GRCm39)	V628D	possibly damaging	Het
Dcaf13	G	A	15: 39,001,637 (GRCm39)	R315Q	possibly damaging	Het
Deaf1	A	G	7: 140,877,486 (GRCm39)	V547A	possibly damaging	Het
Dmxl1	C	T	18: 50,011,698 (GRCm39)	T1285I	probably damaging	Het
Dnah3	C	A	7: 119,646,921 (GRCm39)	W1036L	probably damaging	Het
Dnai3	A	G	3: 145,753,922 (GRCm39)	S713P	probably damaging	Het
Dpy19l1	A	C	9: 24,393,350 (GRCm39)	S140A	possibly damaging	Het
Dsg2	T	A	18: 20,735,302 (GRCm39)	D1093E	possibly damaging	Het
Edaradd	T	A	13: 12,523,304 (GRCm39)	H12L	probably damaging	Het
Enam	T	C	5: 88,640,827 (GRCm39)	M163T	probably benign	Het
Fbxo22	T	A	9: 55,128,333 (GRCm39)	V218E	probably damaging	Het
Fbxo34	A	T	14: 47,767,085 (GRCm39)	K148N	probably damaging	Het
Foxi3	A	G	6: 70,933,960 (GRCm39)	D149G	probably damaging	Het
Gbp8	C	T	5: 105,189,807 (GRCm39)	G75D	probably damaging	Het
Gm10784	C	T	13: 50,099,251 (GRCm39)		noncoding transcript	Het
Gm7247	G	A	14: 51,807,051 (GRCm39)		probably null	Het
Grik3	T	A	4: 125,544,278 (GRCm39)	V333E	probably damaging	Het
Gsap	A	T	5: 21,431,309 (GRCm39)	D211V	probably damaging	Het
H2-T15	T	C	17: 36,368,768 (GRCm39)	Y104C	possibly damaging	Het
Ino80	T	C	2: 119,261,489 (GRCm39)	N725S	probably damaging	Het
Itgad	A	G	7: 127,788,703 (GRCm39)	I443V	probably benign	Het
Itsn1	T	A	16: 91,638,476 (GRCm39)	I52N	probably damaging	Het
Kmt2b	TCTCCTCCTCCTCCTCCTCCTCCTCC	TCTCCTCCTCCTCCTCCTCCTCC	7: 30,285,783 (GRCm39)		probably benign	Het
Lamc1	T	G	1: 153,104,523 (GRCm39)	S59R	probably damaging	Het
Lrrk1	T	C	7: 65,922,801 (GRCm39)	I1366V	probably benign	Het
Lyn	C	A	4: 3,738,850 (GRCm39)	Q41K	probably benign	Het
Mapk13	C	T	17: 28,997,461 (GRCm39)	R360*	probably null	Het
Me1	T	C	9: 86,561,905 (GRCm39)	K60R	probably benign	Het
Mga	T	A	2: 119,771,974 (GRCm39)	D1532E	possibly damaging	Het
Miga1	G	A	3: 151,984,642 (GRCm39)	S427L	probably benign	Het
Mknk2	A	T	10: 80,505,173 (GRCm39)	V186E	probably damaging	Het
Myo1a	C	A	10: 127,546,086 (GRCm39)	N236K	probably benign	Het
Myt1	A	G	2: 181,439,207 (GRCm39)	D243G	probably benign	Het
Ncf4	T	C	15: 78,140,189 (GRCm39)	V196A	possibly damaging	Het
Nlrp5	G	T	7: 23,117,603 (GRCm39)	L442F	probably damaging	Het
Olfm4	G	A	14: 80,258,747 (GRCm39)	D332N	probably benign	Het
Or4a81	C	A	2: 89,619,637 (GRCm39)	V20F	probably benign	Het
Or4b1d	T	A	2: 89,969,432 (GRCm39)	D17V	probably damaging	Het
Osbpl11	T	A	16: 33,016,452 (GRCm39)	M69K	probably benign	Het
Oxnad1	T	A	14: 31,824,366 (GRCm39)	*312K	probably null	Het
Plekhg1	A	G	10: 3,906,985 (GRCm39)	Q634R	probably benign	Het
Plekhh2	T	C	17: 84,882,691 (GRCm39)	Y733H	probably damaging	Het
Ppp1r21	A	G	17: 88,856,941 (GRCm39)	H132R	probably benign	Het
Prkcq	T	C	2: 11,284,333 (GRCm39)	V501A	possibly damaging	Het
Prpf38b	G	A	3: 108,811,408 (GRCm39)		probably benign	Het
Prpf4b	A	T	13: 35,083,954 (GRCm39)	M908L	probably benign	Het
Prps2	T	C	X: 166,135,288 (GRCm39)	D183G	probably damaging	Het
Pudp	T	C	18: 50,701,259 (GRCm39)	D158G	probably damaging	Het
R3hdm1	T	C	1: 128,112,181 (GRCm39)	S422P	probably damaging	Het
Ranbp3	T	A	17: 57,003,640 (GRCm39)		probably null	Het
Ranbp6	A	G	19: 29,787,721 (GRCm39)	V877A	probably benign	Het
Rgsl1	A	T	1: 153,693,328 (GRCm39)	S618T	probably benign	Het
Rhox2a	A	T	X: 36,509,015 (GRCm39)	I61L	probably benign	Het
Rhox2a	G	C	X: 36,508,962 (GRCm39)	R43P	probably benign	Het
Rnf207	A	C	4: 152,396,612 (GRCm39)	D466E	probably benign	Het
Rnf7	A	G	9: 96,353,883 (GRCm39)	I80T	probably benign	Het
Ros1	T	A	10: 52,005,764 (GRCm39)	I878F	possibly damaging	Het
Rsbn1	T	A	3: 103,861,096 (GRCm39)		probably null	Het
Sbk1	T	C	7: 125,890,301 (GRCm39)	S105P	probably damaging	Het
Sdk1	T	C	5: 141,992,380 (GRCm39)	Y790H	probably damaging	Het
Sec62	T	A	3: 30,864,683 (GRCm39)	N182K	unknown	Het
Slc15a1	T	C	14: 121,715,504 (GRCm39)	Y345C	probably damaging	Het
Slc27a1	G	A	8: 72,023,408 (GRCm39)	A15T	probably benign	Het
Slc35d1	A	G	4: 103,070,426 (GRCm39)	F110L	probably damaging	Het
Slc39a5	T	A	10: 128,233,136 (GRCm39)	S333C	probably benign	Het
Tarbp1	A	G	8: 127,173,934 (GRCm39)	S927P	probably damaging	Het
Tns1	A	G	1: 73,992,930 (GRCm39)	S583P	probably damaging	Het
Trim38	A	T	13: 23,966,952 (GRCm39)	D133V	probably damaging	Het
Trim40	T	C	17: 37,193,531 (GRCm39)		probably null	Het
Trim42	C	A	9: 97,244,998 (GRCm39)	V601L	probably benign	Het
Trpc7	G	A	13: 57,035,367 (GRCm39)	R189C	probably damaging	Het
Ttc29	A	T	8: 79,060,208 (GRCm39)	Y376F	probably benign	Het
Vmn2r63	A	G	7: 42,553,114 (GRCm39)	I714T	possibly damaging	Het
Vmn2r68	C	A	7: 84,870,743 (GRCm39)	A847S	probably benign	Het
Zbp1	G	A	2: 173,049,608 (GRCm39)	P385S	possibly damaging	Het
Zfat	A	G	15: 68,056,325 (GRCm39)	Y237H	probably damaging	Het
Zfp236	A	T	18: 82,615,784 (GRCm39)	W1840R	probably damaging	Het
Zfp426	G	T	9: 20,381,923 (GRCm39)	Q341K	possibly damaging	Het
Zfp462	T	A	4: 55,009,349 (GRCm39)	N438K	probably benign	Het
Zfp952	T	A	17: 33,221,899 (GRCm39)	V88E	probably damaging	Het
Znfx1	G	T	2: 166,898,276 (GRCm39)	A216D	probably benign	Het

Other mutations in Mapk8ip3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00578:Mapk8ip3	APN	17	25,119,793 (GRCm39)	missense	probably damaging	1.00
IGL01018:Mapk8ip3	APN	17	25,118,693 (GRCm39)	splice site	probably benign
IGL01066:Mapk8ip3	APN	17	25,120,692 (GRCm39)	missense	probably benign	0.00
IGL01656:Mapk8ip3	APN	17	25,137,003 (GRCm39)	missense	probably damaging	0.99
IGL01991:Mapk8ip3	APN	17	25,146,835 (GRCm39)	missense	possibly damaging	0.78
IGL02014:Mapk8ip3	APN	17	25,122,254 (GRCm39)	splice site	probably benign
IGL02219:Mapk8ip3	APN	17	25,118,532 (GRCm39)	missense	probably damaging	1.00
IGL03006:Mapk8ip3	APN	17	25,120,489 (GRCm39)	missense	probably benign
ANU74:Mapk8ip3	UTSW	17	25,119,551 (GRCm39)	missense	possibly damaging	0.94
FR4737:Mapk8ip3	UTSW	17	25,121,093 (GRCm39)	splice site	probably null
R0028:Mapk8ip3	UTSW	17	25,123,871 (GRCm39)	splice site	probably benign
R0401:Mapk8ip3	UTSW	17	25,128,145 (GRCm39)	intron	probably benign
R0496:Mapk8ip3	UTSW	17	25,133,424 (GRCm39)	splice site	probably benign
R1456:Mapk8ip3	UTSW	17	25,125,923 (GRCm39)	missense	probably damaging	1.00
R1503:Mapk8ip3	UTSW	17	25,123,897 (GRCm39)	missense	probably damaging	1.00
R1554:Mapk8ip3	UTSW	17	25,122,033 (GRCm39)	missense	probably benign	0.14
R1680:Mapk8ip3	UTSW	17	25,119,985 (GRCm39)	missense	probably damaging	1.00
R1733:Mapk8ip3	UTSW	17	25,155,824 (GRCm39)	missense	possibly damaging	0.70
R1741:Mapk8ip3	UTSW	17	25,118,828 (GRCm39)	missense	probably damaging	1.00
R1750:Mapk8ip3	UTSW	17	25,133,433 (GRCm39)	missense	probably null	1.00
R1774:Mapk8ip3	UTSW	17	25,143,119 (GRCm39)	critical splice donor site	probably null
R1845:Mapk8ip3	UTSW	17	25,133,557 (GRCm39)	missense	probably benign	0.29
R1911:Mapk8ip3	UTSW	17	25,123,025 (GRCm39)	missense	probably benign	0.00
R1993:Mapk8ip3	UTSW	17	25,133,562 (GRCm39)	missense	probably damaging	1.00
R2512:Mapk8ip3	UTSW	17	25,133,677 (GRCm39)	nonsense	probably null
R2656:Mapk8ip3	UTSW	17	25,131,781 (GRCm39)	missense	probably damaging	1.00
R2990:Mapk8ip3	UTSW	17	25,124,266 (GRCm39)	missense	probably benign	0.00
R4587:Mapk8ip3	UTSW	17	25,123,761 (GRCm39)	missense	probably damaging	1.00
R4617:Mapk8ip3	UTSW	17	25,123,761 (GRCm39)	missense	probably damaging	1.00
R4627:Mapk8ip3	UTSW	17	25,122,267 (GRCm39)	missense	probably benign
R4868:Mapk8ip3	UTSW	17	25,120,389 (GRCm39)	missense	probably benign	0.04
R4903:Mapk8ip3	UTSW	17	25,120,183 (GRCm39)	missense	probably benign
R4915:Mapk8ip3	UTSW	17	25,128,127 (GRCm39)	missense	possibly damaging	0.75
R5447:Mapk8ip3	UTSW	17	25,118,163 (GRCm39)	missense	probably benign
R5642:Mapk8ip3	UTSW	17	25,122,285 (GRCm39)	missense	possibly damaging	0.63
R6320:Mapk8ip3	UTSW	17	25,125,879 (GRCm39)	missense	probably damaging	0.99
R6900:Mapk8ip3	UTSW	17	25,128,097 (GRCm39)	splice site	probably null
R7178:Mapk8ip3	UTSW	17	25,120,728 (GRCm39)	missense	probably benign	0.02
R7273:Mapk8ip3	UTSW	17	25,125,148 (GRCm39)	missense	probably benign	0.00
R7317:Mapk8ip3	UTSW	17	25,120,692 (GRCm39)	missense	probably benign	0.00
R7323:Mapk8ip3	UTSW	17	25,120,135 (GRCm39)	missense	probably benign
R7701:Mapk8ip3	UTSW	17	25,120,378 (GRCm39)	missense	possibly damaging	0.93
R7873:Mapk8ip3	UTSW	17	25,125,146 (GRCm39)	missense	probably benign	0.01
R8070:Mapk8ip3	UTSW	17	25,120,078 (GRCm39)	critical splice donor site	probably null
R8314:Mapk8ip3	UTSW	17	25,120,748 (GRCm39)	missense	probably benign	0.09
R8356:Mapk8ip3	UTSW	17	25,123,925 (GRCm39)	missense	probably damaging	1.00
R8441:Mapk8ip3	UTSW	17	25,139,474 (GRCm39)	intron	probably benign
R8537:Mapk8ip3	UTSW	17	25,120,652 (GRCm39)	nonsense	probably null
R8802:Mapk8ip3	UTSW	17	25,124,206 (GRCm39)	missense	probably damaging	1.00
R8864:Mapk8ip3	UTSW	17	25,118,492 (GRCm39)	missense	probably damaging	1.00
R8918:Mapk8ip3	UTSW	17	25,131,727 (GRCm39)	missense	probably damaging	1.00
R9312:Mapk8ip3	UTSW	17	25,146,925 (GRCm39)	critical splice acceptor site	probably null
R9599:Mapk8ip3	UTSW	17	25,118,124 (GRCm39)	missense	probably damaging	1.00
R9638:Mapk8ip3	UTSW	17	25,118,023 (GRCm39)	missense	probably benign
R9640:Mapk8ip3	UTSW	17	25,155,647 (GRCm39)	missense	possibly damaging	0.46
R9723:Mapk8ip3	UTSW	17	25,132,585 (GRCm39)	missense	possibly damaging	0.83
X0024:Mapk8ip3	UTSW	17	25,122,947 (GRCm39)	missense	possibly damaging	0.69

Predicted Primers

PCR Primer
(F):5'- AGAAGGTGTCATCTTTCCTGCTC -3'
(R):5'- TCCACCATCTGGCAGTTGTG -3'

Sequencing Primer
(F):5'- TGATAACTTCTGTCCCACAGTACAG -3'
(R):5'- CACCATCTGGCAGTTGTGAGTATG -3'

Posted On

2015-10-08