Mutagenetix > Incidental Mutation

Incidental Mutation 'R6016:Vmn2r68'

478602

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r68

Ensembl Gene

ENSMUSG00000096861

Gene Name

vomeronasal 2, receptor 68

Synonyms

EG620697, Vmn2r68-ps

MMRRC Submission

043255-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R6016 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84870726-84886912 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 84871453 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 610 (I610K)

Ref Sequence

ENSEMBL: ENSMUSP00000129411 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061074]

AlphaFold

L7N2B3

Predicted Effect

probably damaging
Transcript: ENSMUST00000061074
AA Change: I610K

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000129411
Gene: ENSMUSG00000096861
AA Change: I610K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	463	4.5e-28	PFAM
Pfam:NCD3G	507	559	1.1e-18	PFAM
Pfam:7tm_3	589	827	3.7e-53	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.0%
20x: 90.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apol9b	T	C	15: 77,620,058 (GRCm39)	S285P	probably damaging	Het
Casz1	C	A	4: 149,019,041 (GRCm39)	N447K	probably damaging	Het
Cltb	T	C	13: 54,754,480 (GRCm39)	T71A	possibly damaging	Het
Dcaf12	A	G	4: 41,313,267 (GRCm39)	F93L	probably damaging	Het
Dnah5	T	C	15: 28,328,030 (GRCm39)	Y2135H	probably damaging	Het
Entpd2	C	T	2: 25,288,568 (GRCm39)	R191W	probably damaging	Het
Gm17067	G	T	7: 42,357,654 (GRCm39)	P283T	probably benign	Het
Gm3250	A	G	10: 77,618,367 (GRCm39)		probably benign	Het
Gne	T	C	4: 44,039,063 (GRCm39)	E532G	probably damaging	Het
Gsdmc3	T	G	15: 63,740,261 (GRCm39)	D86A	probably benign	Het
Hs3st2	A	G	7: 121,099,922 (GRCm39)	H256R	probably damaging	Het
Il19	T	A	1: 130,863,718 (GRCm39)	D16V	probably damaging	Het
Lats2	A	T	14: 57,971,632 (GRCm39)	N14K	probably damaging	Het
Mill2	G	T	7: 18,590,373 (GRCm39)	S151I	probably benign	Het
Ncapg2	G	A	12: 116,390,227 (GRCm39)	R392H	probably damaging	Het
Nop56	T	C	2: 130,118,545 (GRCm39)		probably null	Het
Or14j8	A	T	17: 38,262,967 (GRCm39)	V316D	probably benign	Het
Or2bd2	A	T	7: 6,443,613 (GRCm39)	H238L	probably benign	Het
Or5p5	A	T	7: 107,414,219 (GRCm39)	I143F	probably benign	Het
Pde1a	T	C	2: 79,695,406 (GRCm39)	R446G	probably benign	Het
Pes1	T	A	11: 3,928,004 (GRCm39)	M552K	possibly damaging	Het
Plxnb2	T	A	15: 89,045,225 (GRCm39)	T1074S	possibly damaging	Het
Psg23	G	T	7: 18,346,112 (GRCm39)	H194Q	probably benign	Het
Rprd2	A	G	3: 95,694,685 (GRCm39)	V116A	probably damaging	Het
Shkbp1	A	G	7: 27,053,826 (GRCm39)	V124A	possibly damaging	Het
Slc38a8	A	T	8: 120,221,044 (GRCm39)		probably null	Het
Slitrk6	A	G	14: 110,987,958 (GRCm39)	V583A	probably benign	Het
Sptbn5	G	T	2: 119,880,573 (GRCm39)		noncoding transcript	Het
Stab1	A	T	14: 30,880,950 (GRCm39)	I614N	probably damaging	Het
Tgm6	A	G	2: 129,983,148 (GRCm39)	T246A	probably damaging	Het
Tnks1bp1	T	C	2: 84,882,734 (GRCm39)	L187P	probably damaging	Het
Vmn1r157	A	G	7: 22,461,272 (GRCm39)	R51G	possibly damaging	Het

Other mutations in Vmn2r68

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Vmn2r68	APN	7	84,886,819 (GRCm39)	missense	probably benign
IGL01477:Vmn2r68	APN	7	84,882,691 (GRCm39)	missense	probably damaging	1.00
IGL01600:Vmn2r68	APN	7	84,871,468 (GRCm39)	missense	probably benign	0.39
IGL01979:Vmn2r68	APN	7	84,871,325 (GRCm39)	missense	probably benign
IGL01999:Vmn2r68	APN	7	84,871,439 (GRCm39)	missense	probably damaging	1.00
IGL02269:Vmn2r68	APN	7	84,870,947 (GRCm39)	missense	possibly damaging	0.84
IGL02517:Vmn2r68	APN	7	84,871,153 (GRCm39)	nonsense	probably null
IGL02827:Vmn2r68	APN	7	84,886,800 (GRCm39)	missense	probably damaging	1.00
IGL02852:Vmn2r68	APN	7	84,882,595 (GRCm39)	missense	probably damaging	1.00
IGL02982:Vmn2r68	APN	7	84,883,649 (GRCm39)	missense	probably benign	0.12
IGL03099:Vmn2r68	APN	7	84,871,448 (GRCm39)	nonsense	probably null
IGL03166:Vmn2r68	APN	7	84,871,331 (GRCm39)	missense	probably benign	0.01
IGL03168:Vmn2r68	APN	7	84,870,972 (GRCm39)	missense	probably damaging	1.00
IGL03243:Vmn2r68	APN	7	84,882,963 (GRCm39)	missense	possibly damaging	0.66
F5770:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
R0280:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0280:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0281:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0281:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0348:Vmn2r68	UTSW	7	84,870,884 (GRCm39)	missense	possibly damaging	0.50
R0390:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0390:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0722:Vmn2r68	UTSW	7	84,870,794 (GRCm39)	missense	possibly damaging	0.95
R1129:Vmn2r68	UTSW	7	84,886,712 (GRCm39)	splice site	probably null
R1136:Vmn2r68	UTSW	7	84,871,549 (GRCm39)	missense	possibly damaging	0.81
R1319:Vmn2r68	UTSW	7	84,881,700 (GRCm39)	missense	probably damaging	0.96
R1614:Vmn2r68	UTSW	7	84,870,946 (GRCm39)	missense	possibly damaging	0.93
R1682:Vmn2r68	UTSW	7	84,882,574 (GRCm39)	missense	possibly damaging	0.68
R1837:Vmn2r68	UTSW	7	84,882,886 (GRCm39)	missense	probably damaging	0.96
R1893:Vmn2r68	UTSW	7	84,883,867 (GRCm39)	nonsense	probably null
R1908:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1909:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1951:Vmn2r68	UTSW	7	84,883,102 (GRCm39)	missense	probably damaging	1.00
R2177:Vmn2r68	UTSW	7	84,871,123 (GRCm39)	missense	probably benign	0.01
R2178:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2185:Vmn2r68	UTSW	7	84,882,901 (GRCm39)	nonsense	probably null
R2188:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2282:Vmn2r68	UTSW	7	84,870,859 (GRCm39)	missense	possibly damaging	0.65
R2567:Vmn2r68	UTSW	7	84,883,803 (GRCm39)	missense	probably benign
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2873:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2874:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R3149:Vmn2r68	UTSW	7	84,886,875 (GRCm39)	missense	probably benign	0.00
R3401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R3978:Vmn2r68	UTSW	7	84,881,670 (GRCm39)	missense	probably benign	0.00
R4399:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4421:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4478:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4479:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4495:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4628:Vmn2r68	UTSW	7	84,883,673 (GRCm39)	missense	probably benign	0.00
R4649:Vmn2r68	UTSW	7	84,870,743 (GRCm39)	missense	probably benign
R4654:Vmn2r68	UTSW	7	84,882,769 (GRCm39)	nonsense	probably null
R4793:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R5007:Vmn2r68	UTSW	7	84,881,622 (GRCm39)	missense	probably benign
R5021:Vmn2r68	UTSW	7	84,882,942 (GRCm39)	missense	possibly damaging	0.62
R5082:Vmn2r68	UTSW	7	84,883,076 (GRCm39)	missense	probably benign	0.12
R5177:Vmn2r68	UTSW	7	84,871,199 (GRCm39)	missense	probably damaging	0.99
R5221:Vmn2r68	UTSW	7	84,871,085 (GRCm39)	missense	probably damaging	1.00
R5514:Vmn2r68	UTSW	7	84,886,767 (GRCm39)	missense	possibly damaging	0.92
R5521:Vmn2r68	UTSW	7	84,882,926 (GRCm39)	missense	probably benign	0.03
R5563:Vmn2r68	UTSW	7	84,871,283 (GRCm39)	missense	probably damaging	1.00
R5664:Vmn2r68	UTSW	7	84,882,978 (GRCm39)	missense	probably benign	0.02
R5829:Vmn2r68	UTSW	7	84,886,812 (GRCm39)	missense	probably benign	0.00
R6356:Vmn2r68	UTSW	7	84,883,048 (GRCm39)	missense	possibly damaging	0.85
R6413:Vmn2r68	UTSW	7	84,870,973 (GRCm39)	missense	probably damaging	1.00
R6418:Vmn2r68	UTSW	7	84,882,915 (GRCm39)	missense	probably benign
R6699:Vmn2r68	UTSW	7	84,881,583 (GRCm39)	missense	possibly damaging	0.58
R7287:Vmn2r68	UTSW	7	84,871,460 (GRCm39)	missense	probably benign	0.33
R7319:Vmn2r68	UTSW	7	84,883,042 (GRCm39)	missense	probably benign
R7374:Vmn2r68	UTSW	7	84,881,607 (GRCm39)	missense	possibly damaging	0.66
R7585:Vmn2r68	UTSW	7	84,881,587 (GRCm39)	missense	probably damaging	1.00
R7605:Vmn2r68	UTSW	7	84,883,116 (GRCm39)	missense	probably benign	0.01
R7892:Vmn2r68	UTSW	7	84,883,722 (GRCm39)	missense	probably benign
R7979:Vmn2r68	UTSW	7	84,883,625 (GRCm39)	critical splice donor site	probably null
R8177:Vmn2r68	UTSW	7	84,871,422 (GRCm39)	nonsense	probably null
R8349:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8378:Vmn2r68	UTSW	7	84,871,108 (GRCm39)	missense	probably benign	0.00
R8397:Vmn2r68	UTSW	7	84,886,722 (GRCm39)	missense	possibly damaging	0.71
R8449:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8543:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R8680:Vmn2r68	UTSW	7	84,871,321 (GRCm39)	missense	possibly damaging	0.68
R9056:Vmn2r68	UTSW	7	84,871,420 (GRCm39)	missense	possibly damaging	0.71
R9342:Vmn2r68	UTSW	7	84,882,993 (GRCm39)	missense	probably benign	0.39
R9734:Vmn2r68	UTSW	7	84,882,757 (GRCm39)	missense	possibly damaging	0.54
V7581:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
Z1176:Vmn2r68	UTSW	7	84,871,289 (GRCm39)	missense	probably benign	0.27
Z1176:Vmn2r68	UTSW	7	84,870,941 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r68	UTSW	7	84,871,307 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- AAGCAGCCACCTCATTCTTC -3'
(R):5'- ACTAGACATGGATCAGTGTGTG -3'

Sequencing Primer
(F):5'- GTAGAACAGTAATTGTCTTAGCCAAG -3'
(R):5'- CTAGACATGGATCAGTGTGTGAAGTG -3'

Posted On

2017-06-26