Mutagenetix > Incidental Mutation

Incidental Mutation 'FR4449:Supt20'

511455

Institutional Source

Beutler Lab

Gene Symbol

Supt20

Ensembl Gene

ENSMUSG00000027751

Gene Name

SPT20 SAGA complex component

Synonyms

p38IP, Fam48a, p38 interacting protein, D3Ertd300e

Accession Numbers

Essential gene?

Probably essential (E-score: 0.939) question?

Stock #

FR4449 ()

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

54600228-54636187 bp(+) (GRCm39)

Type of Mutation

small insertion (2 aa in frame mutation)

DNA Base Change (assembly)

CAGCAG to CAGCAGGAGCAG at 54635070 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000143231 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029315] [ENSMUST00000029316] [ENSMUST00000200441] [ENSMUST00000197502] [ENSMUST00000200439] [ENSMUST00000199674] [ENSMUST00000154787] [ENSMUST00000153224]

AlphaFold

Q7TT00

Predicted Effect

probably benign
Transcript: ENSMUST00000029315

SMART Domains

Protein: ENSMUSP00000029315
Gene: ENSMUSG00000027751

Domain	Start	End	E-Value	Type
low complexity region	65	78	N/A	INTRINSIC
low complexity region	107	159	N/A	INTRINSIC
coiled coil region	201	230	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000029316

SMART Domains

Protein: ENSMUSP00000029316
Gene: ENSMUSG00000027752

Domain	Start	End	E-Value	Type
Pfam:RNase_PH	31	166	2.3e-29	PFAM
Pfam:RNase_PH_C	191	258	8.9e-15	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125632

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127112

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134892

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140935

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142377

Predicted Effect

probably benign
Transcript: ENSMUST00000200441

SMART Domains

Protein: ENSMUSP00000143231
Gene: ENSMUSG00000027751

Domain	Start	End	E-Value	Type
low complexity region	65	78	N/A	INTRINSIC
low complexity region	123	171	N/A	INTRINSIC
coiled coil region	213	242	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000197502

SMART Domains

Protein: ENSMUSP00000143750
Gene: ENSMUSG00000027751

Domain	Start	End	E-Value	Type
low complexity region	45	56	N/A	INTRINSIC
Pfam:Spt20	62	227	1.9e-43	PFAM
low complexity region	424	440	N/A	INTRINSIC
low complexity region	467	476	N/A	INTRINSIC
low complexity region	487	501	N/A	INTRINSIC
low complexity region	512	532	N/A	INTRINSIC
low complexity region	574	587	N/A	INTRINSIC
low complexity region	632	680	N/A	INTRINSIC
coiled coil region	722	751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000200439

SMART Domains

Protein: ENSMUSP00000143059
Gene: ENSMUSG00000027751

Domain	Start	End	E-Value	Type
low complexity region	45	56	N/A	INTRINSIC
Pfam:Spt20	59	227	2.7e-42	PFAM
low complexity region	424	440	N/A	INTRINSIC
low complexity region	467	476	N/A	INTRINSIC
low complexity region	487	501	N/A	INTRINSIC
low complexity region	514	525	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000199674

SMART Domains

Protein: ENSMUSP00000142948
Gene: ENSMUSG00000027751

Domain	Start	End	E-Value	Type
low complexity region	45	56	N/A	INTRINSIC
Pfam:Spt20	59	227	3.3e-39	PFAM
low complexity region	424	442	N/A	INTRINSIC
low complexity region	466	475	N/A	INTRINSIC
low complexity region	486	500	N/A	INTRINSIC
low complexity region	513	524	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000154787

SMART Domains

Protein: ENSMUSP00000115876
Gene: ENSMUSG00000027752

Domain	Start	End	E-Value	Type
Pfam:RNase_PH	19	106	5.7e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150923

Predicted Effect

probably benign
Transcript: ENSMUST00000153224

SMART Domains

Protein: ENSMUSP00000118780
Gene: ENSMUSG00000027752

Domain	Start	End	E-Value	Type
Pfam:RNase_PH	31	130	2e-16	PFAM

Coding Region Coverage

1x: 99.7%
3x: 99.5%
10x: 98.3%
20x: 95.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: The incompletely penetrant homozygous phenotype of a splice-site mutation may include retinal epithelium expansion over the dorsal half of the eye, exencephaly, spina bifida, gastrulation defects and/or aberrant somite and mesoderm development. A few mutants survive postnatally and appear normal. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 128 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap9	GGTATTGCATTTCTTATCT	G	5: 4,031,214 (GRCm39)		probably benign	Homo
Amfr	C	G	8: 94,731,787 (GRCm39)	G30R	probably damaging	Homo
Anxa7	C	T	14: 20,519,479 (GRCm39)	G113E	probably damaging	Homo
Apc	AGC	AGCCAATAACGC	18: 34,415,058 (GRCm39)		probably benign	Het
Apc	AATAAAGC	AATAAAGCCGATAAAGC	18: 34,415,053 (GRCm39)		probably benign	Het
Apol6	TTT	TTTGATT	15: 76,935,643 (GRCm39)		probably null	Homo
Arid1b	CGG	CGGTGG	17: 5,045,864 (GRCm39)		probably benign	Het
B430218F22Rik	CGGCG	CGGCGATGGCG	13: 118,523,387 (GRCm39)		probably benign	Homo
Blm	CCTCCTCCTCCTCCTCCTCCTCCT	CCTCCTCCTCCTTCTCCTCCTCCTCCTCCTCCTCCT	7: 80,162,656 (GRCm39)		probably benign	Het
Brd2	CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	CAAAAAAAAAAAAAAA	17: 34,335,310 (GRCm39)		probably benign	Het
Btnl10	AAG	AAGGAG	11: 58,814,754 (GRCm39)		probably benign	Homo
Cacna1a	ACC	ACCCCC	8: 85,365,343 (GRCm39)		probably benign	Het
Cacna1a	ACC	ACCGCC	8: 85,365,352 (GRCm39)		probably benign	Het
Cacna1a	ACC	ACCCCC	8: 85,365,349 (GRCm39)		probably benign	Het
Calhm1	TGGC	TGGCTGTGGCTGCGGC	19: 47,129,713 (GRCm39)		probably benign	Het
Ccdc15	C	CTTTAT	9: 37,226,454 (GRCm39)		probably null	Het
Ccdc85c	CCG	CCGACG	12: 108,240,875 (GRCm39)		probably benign	Het
Ccnk	TTCCCAC	T	12: 108,168,766 (GRCm39)		probably benign	Het
Cdhr2	AGTC	AGTCGTC	13: 54,873,737 (GRCm39)		probably benign	Homo
Cdk15	A	ATCTAAAAGG	1: 59,296,982 (GRCm39)		probably benign	Homo
Cdx1	GCTG	GCTGCTCCTG	18: 61,152,953 (GRCm39)		probably benign	Het
Cfap46	T	C	7: 139,218,711 (GRCm39)		probably benign	Homo
Cgref1	TTC	TTCGTC	5: 31,091,120 (GRCm39)		probably benign	Het
Cgref1	CTT	CTTATT	5: 31,091,122 (GRCm39)		probably null	Homo
Cluh	G	GACTGAA	11: 74,560,358 (GRCm39)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,080,395 (GRCm39)		probably benign	Het
Cntnap1	AGCC	AGCCCCCGCC	11: 101,080,419 (GRCm39)		probably benign	Het
Cpne1	CCTACT	CCT	2: 155,915,422 (GRCm39)		probably benign	Homo
Cracdl	T	A	1: 37,664,116 (GRCm39)	E594V	probably benign	Homo
Cracdl	C	A	1: 37,664,117 (GRCm39)	E594*	probably null	Homo
Cttnbp2	CTGCTG	CTGCTGTTGCTG	6: 18,367,461 (GRCm39)		probably benign	Het
Cul9	TCC	TCCGCC	17: 46,811,782 (GRCm39)		probably benign	Het
Dbr1	AGGAGG	AGGAGGCGGAGG	9: 99,465,739 (GRCm39)		probably benign	Het
Dbr1	GGAGGA	GGAGGAAGAGGA	9: 99,465,749 (GRCm39)		probably benign	Het
Dbr1	AGGAGG	AGGAGGGGGAGG	9: 99,465,727 (GRCm39)		probably benign	Het
Dhx8	CG	CGAGACAG	11: 101,629,020 (GRCm39)		probably benign	Homo
Dhx8	CG	CGAGACAG	11: 101,629,032 (GRCm39)		probably benign	Het
Dhx8	G	GAGACCC	11: 101,629,033 (GRCm39)		probably benign	Het
Dhx8	AGACCG	AGACCGTGACCG	11: 101,629,010 (GRCm39)		probably benign	Homo
Dhx8	AGACCGGGACCGGGACCGGGACCGGGAC	AGACCGGGACCGGGAC	11: 101,629,016 (GRCm39)		probably benign	Het
Dspp	CGACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAG	CGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAG	5: 104,326,254 (GRCm39)		probably benign	Het
Dusp10	G	T	1: 183,769,253 (GRCm39)	C73F	probably damaging	Homo
Erich3	GA	GAGAA	3: 154,469,150 (GRCm39)		probably benign	Homo
Ermn	CTT	CTTGTT	2: 57,938,086 (GRCm39)		probably benign	Het
Fgd6	GGAT	G	10: 93,880,182 (GRCm39)		probably benign	Homo
G530012D18Rik	GAGAGAGAGAGAGAGAGACAGAGA	GAGAGA	1: 85,504,901 (GRCm39)		probably benign	Homo
Gar1	GCCGCCTCCGCC	GCCGCC	3: 129,624,353 (GRCm39)		probably benign	Homo
Gatad2b	AGAC	A	3: 90,249,224 (GRCm39)		probably benign	Het
Gigyf2	C	T	1: 87,356,307 (GRCm39)		probably benign	Het
Gm16519	A	AGAT	17: 71,236,333 (GRCm39)		probably benign	Homo
Gm4340	AGC	AGCGGC	10: 104,031,946 (GRCm39)		probably benign	Het
Gm4340	GCA	GCATCA	10: 104,031,947 (GRCm39)		probably benign	Het
Gm4340	AGC	AGCGGC	10: 104,031,943 (GRCm39)		probably benign	Het
Gpatch11	AGGAAG	AGGAAGCGGAAG	17: 79,149,597 (GRCm39)		probably benign	Het
Gpatch11	GAAGAG	GAAGAGCAAGAG	17: 79,149,605 (GRCm39)		probably benign	Het
Gpatch11	GG	GGCAGACG	17: 79,149,610 (GRCm39)		probably benign	Het
Hoxa10	T	A	6: 52,211,166 (GRCm39)	Q250L	possibly damaging	Homo
Igkv12-89	GCA	GCAGCAGCAACA	6: 68,812,264 (GRCm39)		probably benign	Homo
Igsf10	G	A	3: 59,226,531 (GRCm39)	R2381C	probably damaging	Homo
Il17rd	GGC	GGCAGC	14: 26,804,635 (GRCm39)		probably benign	Het
Ints5	G	A	19: 8,874,594 (GRCm39)	R851Q	probably benign	Het
Isg20l2	AGA	AGAGGA	3: 87,839,020 (GRCm39)		probably benign	Het
Kcng4	G	T	8: 120,360,258 (GRCm39)	Y39*	probably null	Homo
Kifc5b	A	C	17: 27,143,191 (GRCm39)	E321A	probably benign	Het
Klra2	TCCACAG	TCCACAGAAACCCACAG	6: 131,198,809 (GRCm39)		probably null	Homo
Kmt2b	TCCTCC	TCCTCCCCCTCC	7: 30,285,794 (GRCm39)		probably benign	Het
Kmt2b	CCTCCT	CCTCCTGCTCCT	7: 30,285,786 (GRCm39)		probably benign	Het
Kmt2b	TCCTCC	TCCTCCACCTCC	7: 30,285,791 (GRCm39)		probably benign	Het
Krt10	ACC	ACCACCTCC	11: 99,280,093 (GRCm39)		probably benign	Het
Las1l	GA	GAGAA	X: 94,984,438 (GRCm39)		probably benign	Het
Lce1m	AC	ACTGCTGCTGCCGC	3: 92,925,459 (GRCm39)		probably benign	Het
Leo1	GTACCATGCA	G	9: 75,357,855 (GRCm39)		probably benign	Het
Lkaaear1	CA	CATCTCCAGCTCTA	2: 181,339,364 (GRCm39)		probably benign	Het
Maml2	ACAGCAGCAGCAACAGCAGCAGCAGCAGCA	ACAGCAACAGCAGCAGCAGCAGCA	9: 13,532,752 (GRCm39)		probably benign	Homo
Med12l	CAG	CAGTAG	3: 59,183,384 (GRCm39)		probably null	Het
Mgat4e	GTCGTAGTCATCGT	GTCGT	1: 134,468,735 (GRCm39)		probably benign	Homo
Mn1	GCA	GCAACA	5: 111,567,576 (GRCm39)		probably benign	Het
Morn4	AGGCAGTGAG	AGGCAGTGAGTCTGGCAGTGAG	19: 42,064,548 (GRCm39)		probably benign	Het
Nat8f2	T	A	6: 85,844,668 (GRCm39)	L231F	possibly damaging	Homo
Noc2l	C	CTGA	4: 156,324,558 (GRCm39)		probably benign	Het
Nrg3	T	TAGACAC	14: 38,119,228 (GRCm39)		probably benign	Het
Or8b41	A	G	9: 38,054,484 (GRCm39)	I13V	probably benign	Homo
Piezo1	G	A	8: 123,222,308 (GRCm39)	R503W	probably damaging	Homo
Pih1d2	CTCTTGCGAGGATC	CTC	9: 50,532,927 (GRCm39)		probably null	Homo
Pik3ap1	G	GGAA	19: 41,270,385 (GRCm39)		probably benign	Het
Ppp1r3f	C	A	X: 7,426,575 (GRCm39)	G562V	probably damaging	Homo
Ptms	TTC	TTCGTC	6: 124,891,422 (GRCm39)		probably benign	Het
Ptpn23	G	T	9: 110,216,701 (GRCm39)	P1052T	probably benign	Homo
Qrich2	AACT	A	11: 116,347,025 (GRCm39)		probably benign	Homo
Raet1d	A	ATATCCTCTCTGG	10: 22,246,814 (GRCm39)		probably benign	Het
Rbm33	AGCAGCAGCAGCACCAGCCGCAGCAGCAGCA	AGCAGCAGCAGCA	5: 28,599,166 (GRCm39)		probably benign	Homo
Rrbp1	TGCTTCTCAAAGGTGGCTGCCTTGGCTTC	TGCTTC	2: 143,809,376 (GRCm39)		probably null	Het
Sbp	CAACAAAGATGCTGA	CAACAAAGATGCTGAGAACAAAGATGCTGA	17: 24,164,338 (GRCm39)		probably benign	Het
Setd1a	G	A	7: 127,384,498 (GRCm39)		probably benign	Het
Sfswap	CCCACTCAG	CCCACTCAGTCCACTCAG	5: 129,646,812 (GRCm39)		probably benign	Het
Sfswap	CCACTCAGC	CCACTCAGCTCACTCAGC	5: 129,646,813 (GRCm39)		probably benign	Het
Sh3pxd2b	T	TGTCTGC	11: 32,373,065 (GRCm39)		probably benign	Homo
Six3	CGG	CGGGGG	17: 85,928,790 (GRCm39)		probably benign	Het
Slc12a1	C	CTTTGGCCACAACACG	2: 124,996,136 (GRCm39)		probably benign	Homo
Slc26a8	CTCTCTG	C	17: 28,857,290 (GRCm39)		probably benign	Het
Spata31h1	TTCAGT	TT	10: 82,121,303 (GRCm39)		probably null	Homo
Srebf2	G	T	15: 82,069,536 (GRCm39)	A693S	probably damaging	Homo
Sry	TGCTG	TGCTGCTG	Y: 2,662,832 (GRCm39)		probably benign	Homo
Sry	ACTG	ACTGCTG	Y: 2,662,818 (GRCm39)		probably benign	Het
Ston1	G	A	17: 88,942,953 (GRCm39)	V120M	probably benign	Homo
Tbl3	TG	TGTGG	17: 24,921,518 (GRCm39)		probably benign	Homo
Tctn3	AG	AGAAGCCG	19: 40,595,646 (GRCm39)		probably benign	Het
Tesk1	CCC	CCCACC	4: 43,447,002 (GRCm39)		probably benign	Homo
Tmc2	T	G	2: 130,082,116 (GRCm39)	V433G	probably damaging	Het
Tmprss13	G	A	9: 45,239,856 (GRCm39)	A55T	unknown	Het
Tnfaip8	ACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	AC	18: 50,179,906 (GRCm39)		probably null	Homo
Tob1	CAG	CAGAAG	11: 94,105,294 (GRCm39)		probably benign	Het
Tob1	AGC	AGCCGC	11: 94,105,301 (GRCm39)		probably benign	Het
Tpsab1	TTGCACCTCCT	TT	17: 25,562,756 (GRCm39)		probably benign	Homo
Triobp	GTC	GTCTTC	15: 78,877,589 (GRCm39)		probably benign	Het
Ubtf	CCT	CCTACT	11: 102,197,774 (GRCm39)		probably null	Het
Vps13b	G	T	15: 35,847,103 (GRCm39)	A2629S	probably damaging	Homo
Xpnpep3	G	C	15: 81,311,623 (GRCm39)	D110H	possibly damaging	Het
Zc3h13	CGAGATGTG	CGAGATGTGTGAGATGTG	14: 75,561,041 (GRCm39)		probably null	Homo
Zfhx3	GCAACAGCA	GCAACAGCAACAACAGCA	8: 109,682,726 (GRCm39)		probably benign	Homo
Zfp26	C	A	9: 20,349,842 (GRCm39)	A241S	probably benign	Homo
Zfp335	CTC	CTCATC	2: 164,749,397 (GRCm39)		probably benign	Het
Zfp335	CTCT	CTCTTCT	2: 164,749,403 (GRCm39)		probably benign	Het
Zfp598	ACCACC	ACCACCTCCACC	17: 24,899,750 (GRCm39)		probably benign	Het
Zfp598	ACCACC	ACCACCCCCACC	17: 24,899,759 (GRCm39)		probably benign	Het
Zfp831	TCC	TCCACC	2: 174,487,264 (GRCm39)		probably benign	Het
Zfp831	CTC	CTCGTC	2: 174,487,275 (GRCm39)		probably benign	Het
Zfp978	G	T	4: 147,475,401 (GRCm39)	S316I	probably benign	Het

Other mutations in Supt20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00518:Supt20	APN	3	54,622,590 (GRCm39)	missense	probably damaging	0.98
IGL01781:Supt20	APN	3	54,602,626 (GRCm39)	start codon destroyed	probably null	0.47
IGL02510:Supt20	APN	3	54,622,945 (GRCm39)	intron	probably benign
IGL02656:Supt20	APN	3	54,615,816 (GRCm39)	missense	probably damaging	1.00
IGL02958:Supt20	APN	3	54,621,144 (GRCm39)	intron	probably benign
IGL03036:Supt20	APN	3	54,616,723 (GRCm39)	nonsense	probably null
IGL03128:Supt20	APN	3	54,615,708 (GRCm39)	missense	probably benign	0.05
IGL03164:Supt20	APN	3	54,620,609 (GRCm39)	missense	probably benign	0.01
FR4304:Supt20	UTSW	3	54,635,085 (GRCm39)	nonsense	probably null
FR4304:Supt20	UTSW	3	54,635,068 (GRCm39)	small insertion	probably benign
FR4304:Supt20	UTSW	3	54,635,083 (GRCm39)	small insertion	probably benign
FR4548:Supt20	UTSW	3	54,635,094 (GRCm39)	small insertion	probably benign
FR4548:Supt20	UTSW	3	54,635,078 (GRCm39)	small insertion	probably benign
FR4548:Supt20	UTSW	3	54,635,085 (GRCm39)	small insertion	probably benign
FR4589:Supt20	UTSW	3	54,635,092 (GRCm39)	small insertion	probably benign
FR4589:Supt20	UTSW	3	54,635,072 (GRCm39)	small insertion	probably benign
FR4589:Supt20	UTSW	3	54,635,076 (GRCm39)	small insertion	probably benign
FR4737:Supt20	UTSW	3	54,635,082 (GRCm39)	small insertion	probably benign
FR4737:Supt20	UTSW	3	54,635,078 (GRCm39)	small insertion	probably benign
FR4737:Supt20	UTSW	3	54,635,079 (GRCm39)	small insertion	probably benign
R0383:Supt20	UTSW	3	54,610,570 (GRCm39)	nonsense	probably null
R0675:Supt20	UTSW	3	54,614,390 (GRCm39)	missense	probably damaging	1.00
R0744:Supt20	UTSW	3	54,622,122 (GRCm39)	missense	probably damaging	1.00
R0968:Supt20	UTSW	3	54,615,821 (GRCm39)	intron	probably benign
R1075:Supt20	UTSW	3	54,614,362 (GRCm39)	nonsense	probably null
R1689:Supt20	UTSW	3	54,619,583 (GRCm39)	nonsense	probably null
R1772:Supt20	UTSW	3	54,617,841 (GRCm39)	missense	probably damaging	1.00
R1779:Supt20	UTSW	3	54,622,164 (GRCm39)	missense	probably benign	0.00
R1829:Supt20	UTSW	3	54,635,079 (GRCm39)	utr 3 prime	probably benign
R3236:Supt20	UTSW	3	54,616,501 (GRCm39)	missense	possibly damaging	0.94
R3237:Supt20	UTSW	3	54,616,501 (GRCm39)	missense	possibly damaging	0.94
R4989:Supt20	UTSW	3	54,602,555 (GRCm39)	utr 5 prime	probably benign
R5180:Supt20	UTSW	3	54,616,506 (GRCm39)	missense	probably benign	0.00
R5188:Supt20	UTSW	3	54,617,849 (GRCm39)	missense	possibly damaging	0.87
R5423:Supt20	UTSW	3	54,616,746 (GRCm39)	missense	probably damaging	1.00
R5627:Supt20	UTSW	3	54,620,611 (GRCm39)	missense	possibly damaging	0.86
R5888:Supt20	UTSW	3	54,619,628 (GRCm39)	missense	probably benign
R5995:Supt20	UTSW	3	54,616,474 (GRCm39)	missense	probably damaging	0.97
R6316:Supt20	UTSW	3	54,635,069 (GRCm39)	small insertion	probably benign
R6623:Supt20	UTSW	3	54,625,715 (GRCm39)	missense	possibly damaging	0.93
R6713:Supt20	UTSW	3	54,606,022 (GRCm39)	missense	possibly damaging	0.89
R6874:Supt20	UTSW	3	54,635,175 (GRCm39)	splice site	probably null
R6988:Supt20	UTSW	3	54,606,018 (GRCm39)	missense	probably damaging	1.00
R7149:Supt20	UTSW	3	54,635,832 (GRCm39)	missense	unknown
R7592:Supt20	UTSW	3	54,614,543 (GRCm39)	missense	probably damaging	0.97
R7940:Supt20	UTSW	3	54,620,620 (GRCm39)	missense	probably benign	0.04
R8480:Supt20	UTSW	3	54,614,537 (GRCm39)	missense	probably damaging	1.00
R8550:Supt20	UTSW	3	54,623,063 (GRCm39)	missense	possibly damaging	0.48
R8935:Supt20	UTSW	3	54,634,988 (GRCm39)	critical splice acceptor site	probably null
R9412:Supt20	UTSW	3	54,635,069 (GRCm39)	small deletion	probably benign
R9414:Supt20	UTSW	3	54,610,504 (GRCm39)	missense	probably damaging	1.00
R9694:Supt20	UTSW	3	54,623,015 (GRCm39)	missense	probably benign	0.02
RF001:Supt20	UTSW	3	54,635,083 (GRCm39)	small insertion	probably benign
RF009:Supt20	UTSW	3	54,635,083 (GRCm39)	small insertion	probably benign
RF010:Supt20	UTSW	3	54,635,083 (GRCm39)	small insertion	probably benign
RF014:Supt20	UTSW	3	54,635,086 (GRCm39)	small insertion	probably benign
RF026:Supt20	UTSW	3	54,635,091 (GRCm39)	nonsense	probably null
RF026:Supt20	UTSW	3	54,635,068 (GRCm39)	small insertion	probably benign
RF032:Supt20	UTSW	3	54,635,087 (GRCm39)	small insertion	probably benign
RF038:Supt20	UTSW	3	54,635,068 (GRCm39)	small insertion	probably benign
RF045:Supt20	UTSW	3	54,635,087 (GRCm39)	small insertion	probably benign
RF052:Supt20	UTSW	3	54,635,086 (GRCm39)	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- GATACTCGAATTGTACTGCTTCTAG -3'
(R):5'- TGGAATGTGCCACCAAGACC -3'

Sequencing Primer
(F):5'- CGAATTGTACTGCTTCTAGTTTTTG -3'
(R):5'- GCTGACTGGCTTTTAACATGTCAGAC -3'

Posted On

2018-04-05