Mutagenetix > Incidental Mutation

Incidental Mutation 'R1190:Fstl4'

100743

Institutional Source

Beutler Lab

Gene Symbol

Fstl4

Ensembl Gene

ENSMUSG00000036264

Gene Name

follistatin-like 4

Synonyms

SPIG1, B230374F23Rik

MMRRC Submission

039262-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.151) question?

Stock #

R1190 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

52655461-53079365 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 52959373 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 138 (M138T)

Ref Sequence

ENSEMBL: ENSMUSP00000042007 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036796]

AlphaFold

Q5STE3

Predicted Effect

probably benign
Transcript: ENSMUST00000036796
AA Change: M138T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000042007
Gene: ENSMUSG00000036264
AA Change: M138T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
KAZAL	87	132	4.7e-13	SMART
Blast:IG_like	215	241	6e-7	BLAST
IGc2	260	327	1.9e-6	SMART
IGc2	352	419	1e-14	SMART

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.3%
20x: 89.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous null mice were born at expected Mendelian ratio and healthy, fertile, apparently normal with normal retinal laminar structure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 20 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Antxrl	T	C	14: 33,791,207 (GRCm39)	F367L	probably benign	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Atp8a2	T	C	14: 60,097,719 (GRCm39)	K770E	probably benign	Het
Dnah17	G	A	11: 117,933,001 (GRCm39)	R3586W	probably damaging	Het
Dpysl2	A	G	14: 67,061,850 (GRCm39)	V252A	probably benign	Het
Dus2	T	C	8: 106,771,497 (GRCm39)	S208P	possibly damaging	Het
Elk3	T	C	10: 93,101,058 (GRCm39)	N231S	probably benign	Het
Ephx1	A	T	1: 180,821,494 (GRCm39)	M280K	probably benign	Het
Iqsec1	A	T	6: 90,666,659 (GRCm39)	Y593N	probably damaging	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Lep	G	T	6: 29,071,173 (GRCm39)	E166*	probably null	Het
Limch1	A	G	5: 67,126,540 (GRCm39)	D56G	probably damaging	Het
Or4d10c	G	T	19: 12,066,051 (GRCm39)	T35K	possibly damaging	Het
Pkn2	A	T	3: 142,517,286 (GRCm39)		probably null	Het
Plxna4	A	G	6: 32,228,071 (GRCm39)	Y512H	probably damaging	Het
Tekt1	A	T	11: 72,246,039 (GRCm39)	I161N	probably damaging	Het
Tex14	T	G	11: 87,385,934 (GRCm39)		probably null	Het
Trpc3	T	C	3: 36,725,497 (GRCm39)	N160D	probably benign	Het
Zfp369	T	C	13: 65,440,107 (GRCm39)	S264P	probably damaging	Het
Zfp747	G	A	7: 126,973,726 (GRCm39)	A148V	probably damaging	Het

Other mutations in Fstl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00093:Fstl4	APN	11	53,077,102 (GRCm39)	missense	probably benign	0.01
IGL00885:Fstl4	APN	11	53,039,809 (GRCm39)	missense	possibly damaging	0.90
IGL00915:Fstl4	APN	11	53,067,825 (GRCm39)	missense	probably benign
IGL00933:Fstl4	APN	11	53,077,588 (GRCm39)	missense	possibly damaging	0.80
IGL01022:Fstl4	APN	11	53,077,568 (GRCm39)	missense	probably benign	0.18
IGL01121:Fstl4	APN	11	52,705,464 (GRCm39)	missense	probably benign	0.00
IGL01656:Fstl4	APN	11	52,891,201 (GRCm39)	missense	probably damaging	1.00
IGL01805:Fstl4	APN	11	53,077,184 (GRCm39)	missense	probably damaging	1.00
IGL01997:Fstl4	APN	11	53,053,881 (GRCm39)	nonsense	probably null
IGL02386:Fstl4	APN	11	52,664,698 (GRCm39)	missense	probably benign	0.21
IGL02536:Fstl4	APN	11	53,024,851 (GRCm39)	splice site	probably benign
IGL02807:Fstl4	APN	11	53,077,501 (GRCm39)	missense	probably benign	0.03
IGL03037:Fstl4	APN	11	53,059,050 (GRCm39)	missense	possibly damaging	0.83
R0462:Fstl4	UTSW	11	53,077,229 (GRCm39)	missense	probably benign	0.09
R1300:Fstl4	UTSW	11	52,959,454 (GRCm39)	missense	probably benign
R1626:Fstl4	UTSW	11	52,891,117 (GRCm39)	nonsense	probably null
R1695:Fstl4	UTSW	11	53,056,705 (GRCm39)	splice site	probably null
R1699:Fstl4	UTSW	11	53,059,005 (GRCm39)	missense	possibly damaging	0.81
R1727:Fstl4	UTSW	11	52,959,478 (GRCm39)	missense	probably damaging	1.00
R1752:Fstl4	UTSW	11	53,077,622 (GRCm39)	missense	probably benign	0.09
R1866:Fstl4	UTSW	11	53,077,225 (GRCm39)	missense	probably benign	0.00
R4689:Fstl4	UTSW	11	52,959,477 (GRCm39)	nonsense	probably null
R5126:Fstl4	UTSW	11	53,077,388 (GRCm39)	missense	possibly damaging	0.71
R5129:Fstl4	UTSW	11	53,077,266 (GRCm39)	missense	probably damaging	1.00
R5499:Fstl4	UTSW	11	52,959,374 (GRCm39)	missense	probably benign	0.01
R5578:Fstl4	UTSW	11	53,056,608 (GRCm39)	missense	probably damaging	1.00
R5715:Fstl4	UTSW	11	52,891,243 (GRCm39)	missense	possibly damaging	0.53
R6125:Fstl4	UTSW	11	53,077,130 (GRCm39)	missense	probably benign
R6177:Fstl4	UTSW	11	53,059,031 (GRCm39)	missense	probably benign	0.00
R6236:Fstl4	UTSW	11	53,077,162 (GRCm39)	missense	probably benign	0.00
R6311:Fstl4	UTSW	11	53,067,804 (GRCm39)	missense	probably damaging	1.00
R6611:Fstl4	UTSW	11	53,077,552 (GRCm39)	missense	probably benign	0.01
R6886:Fstl4	UTSW	11	53,077,277 (GRCm39)	missense	probably damaging	1.00
R7404:Fstl4	UTSW	11	53,024,898 (GRCm39)	missense	probably benign	0.03
R7423:Fstl4	UTSW	11	52,959,382 (GRCm39)	missense	possibly damaging	0.54
R7586:Fstl4	UTSW	11	52,963,256 (GRCm39)	missense	probably benign	0.00
R7756:Fstl4	UTSW	11	53,059,123 (GRCm39)	missense	possibly damaging	0.69
R7758:Fstl4	UTSW	11	53,059,123 (GRCm39)	missense	possibly damaging	0.69
R7775:Fstl4	UTSW	11	53,067,798 (GRCm39)	nonsense	probably null
R7953:Fstl4	UTSW	11	52,891,050 (GRCm39)	missense	probably benign	0.01
R8026:Fstl4	UTSW	11	52,959,496 (GRCm39)	missense	probably damaging	0.99
R8043:Fstl4	UTSW	11	52,891,050 (GRCm39)	missense	probably benign	0.01
R8375:Fstl4	UTSW	11	53,053,502 (GRCm39)	missense	possibly damaging	0.63
R8866:Fstl4	UTSW	11	52,963,233 (GRCm39)	missense	possibly damaging	0.54
R9103:Fstl4	UTSW	11	52,664,696 (GRCm39)	missense	probably benign	0.21
R9182:Fstl4	UTSW	11	53,024,905 (GRCm39)	missense	probably damaging	0.98
R9297:Fstl4	UTSW	11	53,024,973 (GRCm39)	missense	possibly damaging	0.50
R9390:Fstl4	UTSW	11	52,891,102 (GRCm39)	missense	probably benign
R9396:Fstl4	UTSW	11	52,664,778 (GRCm39)	missense	probably benign
R9447:Fstl4	UTSW	11	53,077,166 (GRCm39)	missense	probably damaging	1.00
R9506:Fstl4	UTSW	11	53,024,950 (GRCm39)	missense	probably benign	0.18
R9518:Fstl4	UTSW	11	53,056,647 (GRCm39)	missense	possibly damaging	0.71
R9523:Fstl4	UTSW	11	53,075,466 (GRCm39)	missense	probably benign	0.04
R9586:Fstl4	UTSW	11	53,077,729 (GRCm39)	missense	probably benign	0.02
R9594:Fstl4	UTSW	11	52,664,694 (GRCm39)	missense	probably benign
X0013:Fstl4	UTSW	11	53,053,446 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGAACCCTGCTCTATGATGGTGAC -3'
(R):5'- ACATTCTCAGAGGCGCTGACAAG -3'

Sequencing Primer
(F):5'- CTCTATGATGGTGACACTGTTTAG -3'
(R):5'- GCTGACAAGCTGTGAGTCC -3'

Posted On

2014-01-15