Mutagenetix > Incidental Mutation

Incidental Mutation 'R0383:Tead3'

31131

Institutional Source

Beutler Lab

Gene Symbol

Tead3

Ensembl Gene

ENSMUSG00000002249

Gene Name

TEA domain family member 3

Synonyms

DTEF-1, Tcf13r2, TEF-5, ETFR-1, TEAD-3

MMRRC Submission

038589-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0383 (G1)

Quality Score

214

Status

Not validated

Chromosome

Chromosomal Location

28550645-28569779 bp(-) (GRCm39)

Type of Mutation

splice site (6 bp from exon)

DNA Base Change (assembly)

A to G at 28553672 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152030 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042334] [ENSMUST00000114799] [ENSMUST00000129935] [ENSMUST00000154873] [ENSMUST00000156862] [ENSMUST00000156862] [ENSMUST00000219703]

AlphaFold

P70210

Predicted Effect

probably benign
Transcript: ENSMUST00000042334

SMART Domains

Protein: ENSMUSP00000048469
Gene: ENSMUSG00000037805

Domain	Start	End	E-Value	Type
Pfam:Ribosomal_L1	12	213	3.5e-49	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000080572

SMART Domains

Protein: ENSMUSP00000079410
Gene: ENSMUSG00000002249

Domain	Start	End	E-Value	Type
TEA	26	97	9.04e-52	SMART
low complexity region	124	139	N/A	INTRINSIC
low complexity region	155	176	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	201	218	N/A	INTRINSIC
PDB:3KYS\|C	222	439	1e-121	PDB

Predicted Effect

probably null
Transcript: ENSMUST00000114799

SMART Domains

Protein: ENSMUSP00000110447
Gene: ENSMUSG00000002249

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
TEA	52	123	9.04e-52	SMART
low complexity region	150	165	N/A	INTRINSIC
low complexity region	181	202	N/A	INTRINSIC
low complexity region	208	222	N/A	INTRINSIC
low complexity region	227	244	N/A	INTRINSIC
PDB:3KYS\|C	248	465	1e-120	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127212

Predicted Effect

probably benign
Transcript: ENSMUST00000129935

SMART Domains

Protein: ENSMUSP00000114141
Gene: ENSMUSG00000037805

Domain	Start	End	E-Value	Type
Pfam:Ribosomal_L1	3	57	1.3e-8	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141049

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146668

Predicted Effect

probably null
Transcript: ENSMUST00000154873

SMART Domains

Protein: ENSMUSP00000118582
Gene: ENSMUSG00000002249

Domain	Start	End	E-Value	Type
Pfam:TEA	1	366	3.8e-149	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000156862

SMART Domains

Protein: ENSMUSP00000115443
Gene: ENSMUSG00000002249

Domain	Start	End	E-Value	Type
Pfam:TEA	1	366	3.8e-149	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000156862

SMART Domains

Protein: ENSMUSP00000115443
Gene: ENSMUSG00000002249

Domain	Start	End	E-Value	Type
Pfam:TEA	1	366	3.8e-149	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151557

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150103

Predicted Effect

probably null
Transcript: ENSMUST00000219703

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000226172

Coding Region Coverage

1x: 99.0%
3x: 97.9%
10x: 95.2%
20x: 88.6%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and thought to play a role in placental gene regulation and development. Alternative splicing, and alternate use of an upstream AUG translation initiation codon, and an in-frame downstream non-AUG (AUA) codon, results in 2 isoforms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730455P16Rik	G	T	11: 80,254,767 (GRCm39)	Y351*	probably null	Het
Aadac	G	T	3: 59,943,368 (GRCm39)	R91L	possibly damaging	Het
Adgrg1	T	A	8: 95,738,370 (GRCm39)	F621Y	probably damaging	Het
Ankmy1	G	T	1: 92,812,775 (GRCm39)	D511E	probably benign	Het
Anks4b	A	T	7: 119,782,097 (GRCm39)	D376V	probably damaging	Het
Aox1	G	T	1: 58,100,400 (GRCm39)	C399F	probably benign	Het
Arfgef1	C	T	1: 10,269,067 (GRCm39)		probably null	Het
Arhgef4	G	A	1: 34,849,614 (GRCm39)	V546M	probably damaging	Het
Cab39	T	A	1: 85,765,020 (GRCm39)	V98E	probably damaging	Het
Cacna1b	T	C	2: 24,651,856 (GRCm39)	N108D	probably damaging	Het
Car15	C	A	16: 17,654,617 (GRCm39)	E134*	probably null	Het
Ccdc80	T	G	16: 44,915,732 (GRCm39)	Y163D	probably damaging	Het
Cdcp3	A	G	7: 130,841,268 (GRCm39)	M537V	probably benign	Het
Col22a1	C	T	15: 71,740,853 (GRCm39)	G513D	unknown	Het
Col8a1	T	C	16: 57,452,805 (GRCm39)	D66G	probably damaging	Het
Crot	C	A	5: 9,018,734 (GRCm39)	S544I	probably damaging	Het
Cubn	G	T	2: 13,435,770 (GRCm39)	P1062Q	probably damaging	Het
Dcc	A	T	18: 71,553,334 (GRCm39)	V774E	probably damaging	Het
Dlgap5	T	A	14: 47,647,818 (GRCm39)	M240L	probably benign	Het
Dlx4	A	G	11: 95,036,261 (GRCm39)	V16A	probably benign	Het
Dnah17	G	T	11: 117,958,373 (GRCm39)	H2703Q	probably benign	Het
Duox2	A	G	2: 122,122,291 (GRCm39)		probably null	Het
Fn1	C	T	1: 71,636,844 (GRCm39)	V168I	probably damaging	Het
Fpr-rs4	A	T	17: 18,242,359 (GRCm39)	D122V	probably damaging	Het
Gas2l2	A	T	11: 83,313,923 (GRCm39)	I463N	probably benign	Het
Ggta1	G	T	2: 35,292,416 (GRCm39)	P297Q	probably damaging	Het
Gpatch3	C	A	4: 133,305,457 (GRCm39)	R231S	probably damaging	Het
Gpc1	T	C	1: 92,782,705 (GRCm39)	Y151H	probably damaging	Het
Gpr141b	A	G	13: 19,913,317 (GRCm39)		noncoding transcript	Het
Gtf2e2	T	C	8: 34,245,973 (GRCm39)	W119R	probably damaging	Het
H2-M10.2	T	C	17: 36,595,253 (GRCm39)	I304V	probably benign	Het
Helq	T	C	5: 100,927,031 (GRCm39)	K685R	probably benign	Het
Hps5	C	T	7: 46,418,712 (GRCm39)		probably null	Het
Iars1	T	C	13: 49,885,818 (GRCm39)	C1186R	probably damaging	Het
Ift43	T	C	12: 86,208,795 (GRCm39)	V158A	possibly damaging	Het
Ift70b	A	G	2: 75,768,586 (GRCm39)	Y56H	probably damaging	Het
Iqca1	A	T	1: 90,070,429 (GRCm39)	I141N	probably damaging	Het
Kat6b	A	G	14: 21,719,149 (GRCm39)	N1276S	probably benign	Het
Kif19a	A	T	11: 114,656,340 (GRCm39)	M1L	possibly damaging	Het
Kif1b	T	G	4: 149,286,969 (GRCm39)	H1241P	probably damaging	Het
Kif26a	T	C	12: 112,144,510 (GRCm39)	V1588A	possibly damaging	Het
Klb	T	A	5: 65,529,842 (GRCm39)		probably null	Het
Krtap26-1	A	T	16: 88,444,131 (GRCm39)	Y163*	probably null	Het
Lefty1	G	T	1: 180,765,199 (GRCm39)	E256*	probably null	Het
Lox	T	C	18: 52,662,271 (GRCm39)	N44S	possibly damaging	Het
Marf1	C	T	16: 13,960,398 (GRCm39)	A549T	probably damaging	Het
Mctp1	A	G	13: 76,949,663 (GRCm39)	Y565C	probably damaging	Het
Megf6	C	T	4: 154,349,783 (GRCm39)	A961V	probably benign	Het
Mindy4	A	G	6: 55,253,619 (GRCm39)	K496R	probably benign	Het
Nalcn	A	T	14: 123,744,971 (GRCm39)	H352Q	probably benign	Het
Ncoa5	T	C	2: 164,851,310 (GRCm39)	I188V	possibly damaging	Het
Notum	G	T	11: 120,545,282 (GRCm39)	H426N	probably benign	Het
Or52r1	T	A	7: 102,536,458 (GRCm39)	I301F	possibly damaging	Het
Orm2	A	T	4: 63,282,233 (GRCm39)	D137V	probably damaging	Het
Pabpc2	G	A	18: 39,908,448 (GRCm39)	G571D	probably damaging	Het
Pabpc4	A	G	4: 123,191,735 (GRCm39)	N599S	probably damaging	Het
Pak1ip1	T	C	13: 41,166,080 (GRCm39)	V335A	probably benign	Het
Pcdhb11	A	C	18: 37,556,446 (GRCm39)	D592A	probably damaging	Het
Pmch	C	A	10: 87,927,120 (GRCm39)	T41K	possibly damaging	Het
Polb	G	T	8: 23,130,011 (GRCm39)	S187*	probably null	Het
Pter	G	T	2: 13,005,753 (GRCm39)	G309*	probably null	Het
Ptprg	T	C	14: 12,219,024 (GRCm38)	V406A	possibly damaging	Het
Ranbp3l	A	T	15: 9,063,184 (GRCm39)	E467V	possibly damaging	Het
Rif1	T	A	2: 51,975,153 (GRCm39)	M354K	probably damaging	Het
Ripk4	C	T	16: 97,549,312 (GRCm39)	C248Y	probably damaging	Het
Slc6a15	T	C	10: 103,253,914 (GRCm39)	W617R	probably damaging	Het
Smyd5	C	T	6: 85,417,155 (GRCm39)	Q178*	probably null	Het
St18	T	A	1: 6,873,248 (GRCm39)	F328I	probably damaging	Het
Supt20	T	A	3: 54,610,570 (GRCm39)	L124*	probably null	Het
Tarbp1	T	A	8: 127,174,223 (GRCm39)	H861L	probably benign	Het
Tars1	A	G	15: 11,390,411 (GRCm39)	M356T	probably benign	Het
Tbc1d10a	A	G	11: 4,162,819 (GRCm39)	T221A	probably damaging	Het
Tprg1	A	G	16: 25,240,985 (GRCm39)	T254A	probably damaging	Het
Trank1	C	A	9: 111,220,545 (GRCm39)	N2427K	probably benign	Het
Tufm	T	C	7: 126,089,036 (GRCm39)	S380P	probably damaging	Het
Tyrobp	C	T	7: 30,114,042 (GRCm39)	R68C	probably damaging	Het
Ubl4b	T	C	3: 107,462,143 (GRCm39)	E39G	possibly damaging	Het
Uggt2	A	T	14: 119,286,863 (GRCm39)	F661I	probably damaging	Het
Upf3b	A	G	X: 36,368,120 (GRCm39)	I144T	probably benign	Het
Usp54	A	T	14: 20,611,320 (GRCm39)	D1165E	probably benign	Het
Vmn2r81	C	T	10: 79,129,281 (GRCm39)	T724I	possibly damaging	Het
Vsig1	A	G	X: 139,837,062 (GRCm39)	I247M	possibly damaging	Het
Zfp110	C	A	7: 12,583,187 (GRCm39)	L612I	probably benign	Het
Zfp318	C	A	17: 46,724,222 (GRCm39)	T2075K	probably damaging	Het
Zfp37	A	G	4: 62,110,122 (GRCm39)	M1T	probably null	Het
Zfp605	T	A	5: 110,276,720 (GRCm39)	C613S	probably damaging	Het
Zfp729a	G	A	13: 67,769,792 (GRCm39)	P146S	possibly damaging	Het
Zfp85	T	C	13: 67,896,791 (GRCm39)	N427S	probably benign	Het

Other mutations in Tead3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Tead3	APN	17	28,551,780 (GRCm39)	missense	possibly damaging	0.91
IGL01752:Tead3	APN	17	28,552,568 (GRCm39)	missense	probably damaging	1.00
IGL01760:Tead3	APN	17	28,552,055 (GRCm39)	missense	probably benign	0.07
IGL02868:Tead3	APN	17	28,552,069 (GRCm39)	nonsense	probably null
IGL02932:Tead3	APN	17	28,560,325 (GRCm39)	missense	probably damaging	1.00
R0015:Tead3	UTSW	17	28,560,325 (GRCm39)	missense	probably damaging	1.00
R0376:Tead3	UTSW	17	28,560,339 (GRCm39)	missense	probably damaging	0.98
R1203:Tead3	UTSW	17	28,560,536 (GRCm39)	missense	probably benign	0.06
R1699:Tead3	UTSW	17	28,553,698 (GRCm39)	missense	possibly damaging	0.52
R2037:Tead3	UTSW	17	28,555,544 (GRCm39)	missense	probably damaging	0.98
R2148:Tead3	UTSW	17	28,552,638 (GRCm39)	missense	probably damaging	1.00
R4871:Tead3	UTSW	17	28,553,962 (GRCm39)	missense	probably benign	0.42
R4871:Tead3	UTSW	17	28,552,589 (GRCm39)	missense	probably damaging	1.00
R5070:Tead3	UTSW	17	28,560,451 (GRCm39)	missense	probably benign	0.06
R5557:Tead3	UTSW	17	28,555,244 (GRCm39)	intron	probably benign
R5891:Tead3	UTSW	17	28,560,339 (GRCm39)	missense	probably damaging	0.98
R5991:Tead3	UTSW	17	28,553,352 (GRCm39)	splice site	probably null
R6335:Tead3	UTSW	17	28,552,299 (GRCm39)	missense	probably damaging	1.00
R6999:Tead3	UTSW	17	28,560,506 (GRCm39)	missense	probably benign	0.00
R7165:Tead3	UTSW	17	28,552,228 (GRCm39)	missense	probably benign	0.00
R7718:Tead3	UTSW	17	28,552,491 (GRCm39)	missense	probably damaging	1.00
R7743:Tead3	UTSW	17	28,551,801 (GRCm39)	missense	probably benign	0.06
R8025:Tead3	UTSW	17	28,554,009 (GRCm39)	missense	probably benign	0.23
R8034:Tead3	UTSW	17	28,552,203 (GRCm39)	missense	probably damaging	1.00
R8851:Tead3	UTSW	17	28,551,704 (GRCm39)	missense	probably damaging	0.97
R9245:Tead3	UTSW	17	28,551,709 (GRCm39)	missense	probably benign	0.34
R9262:Tead3	UTSW	17	28,560,495 (GRCm39)	missense	probably benign	0.01
X0066:Tead3	UTSW	17	28,560,401 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATAAAGCAGGTTAGAGGTCTTCCCC -3'
(R):5'- GCGTTCTACAGAACAAGTTCAGCCC -3'

Sequencing Primer
(F):5'- CCTGATCCGTTGTCTCAAGG -3'
(R):5'- TCCTCCTCCTCAAGGGTAGG -3'

Posted On

2013-04-24