Incidental Mutation 'R5089:Kras'
ID 387636
Institutional Source Beutler Lab
Gene Symbol Kras
Ensembl Gene ENSMUSG00000030265
Gene Name Kirsten rat sarcoma viral oncogene homolog
Synonyms Kras2, Kras-2, K-ras, Ki-ras
MMRRC Submission 042678-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R5089 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 145162425-145195965 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 145170869 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Lysine to Glutamic Acid at position 169 (K169E)
Ref Sequence ENSEMBL: ENSMUSP00000107339 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032399] [ENSMUST00000111710] [ENSMUST00000156486] [ENSMUST00000203147]
AlphaFold P32883
Predicted Effect probably benign
Transcript: ENSMUST00000032399
SMART Domains Protein: ENSMUSP00000032399
Gene: ENSMUSG00000030265

DomainStartEndE-ValueType
RAS 1 166 1.14e-123 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000111710
AA Change: K169E

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
SMART Domains Protein: ENSMUSP00000107339
Gene: ENSMUSG00000030265
AA Change: K169E

DomainStartEndE-ValueType
RAS 1 166 3.7e-123 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123972
Predicted Effect noncoding transcript
Transcript: ENSMUST00000149314
Predicted Effect probably benign
Transcript: ENSMUST00000156486
Predicted Effect probably benign
Transcript: ENSMUST00000203147
SMART Domains Protein: ENSMUSP00000145294
Gene: ENSMUSG00000030265

DomainStartEndE-ValueType
small_GTPase 1 53 3.1e-8 SMART
Meta Mutation Damage Score 0.0721 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.3%
  • 10x: 96.0%
  • 20x: 91.3%
Validation Efficiency 98% (65/66)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality, decreased fetal growth, pericardial edema, anemia, and liver hypoplasia. Mice heterozygous for various knock-in alleles exhibit increased tumorigenesis. [provided by MGI curators]
Allele List at MGI

All alleles(26) : Targeted, knock-out(3) Targeted, other(7) Gene trapped(14) Other(2)
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700030J22Rik T C 8: 117,698,672 (GRCm39) N145S possibly damaging Het
Acp2 T C 2: 91,042,267 (GRCm39) probably benign Het
Adgrf2 G A 17: 43,020,988 (GRCm39) A612V probably benign Het
Bltp1 T A 3: 37,041,651 (GRCm39) D2676E probably benign Het
Cct3 T G 3: 88,208,150 (GRCm39) M46R probably damaging Het
Cdc123 T C 2: 5,809,811 (GRCm39) D200G probably benign Het
Cdh9 T A 15: 16,778,362 (GRCm39) F59Y probably damaging Het
Cluh A G 11: 74,551,198 (GRCm39) E349G probably damaging Het
Col16a1 C T 4: 129,972,988 (GRCm39) T643M probably benign Het
Col5a1 G A 2: 27,908,614 (GRCm39) W67* probably null Het
Crbn T C 6: 106,758,679 (GRCm39) H381R possibly damaging Het
Crim1 A G 17: 78,681,519 (GRCm39) D991G probably damaging Het
Dhx16 A T 17: 36,194,981 (GRCm39) M503L probably damaging Het
Dthd1 A G 5: 63,007,248 (GRCm39) T650A probably benign Het
Etfa T A 9: 55,396,150 (GRCm39) K139* probably null Het
Flnc T C 6: 29,447,812 (GRCm39) I1205T probably damaging Het
Fzd6 T A 15: 38,870,875 (GRCm39) C32S probably damaging Het
Gm1110 T A 9: 26,793,683 (GRCm39) D515V probably damaging Het
Gm7676 T C 8: 13,946,401 (GRCm39) noncoding transcript Het
Gpr157 T C 4: 150,186,750 (GRCm39) S293P possibly damaging Het
Hc T C 2: 34,914,902 (GRCm39) D810G probably benign Het
Helz2 T C 2: 180,876,942 (GRCm39) H1184R probably benign Het
Hoxc5 A T 15: 102,922,487 (GRCm39) probably benign Het
Iah1 C T 12: 21,373,309 (GRCm39) S196L possibly damaging Het
Il5 C T 11: 53,612,655 (GRCm39) T55I possibly damaging Het
Larp1 C A 11: 57,938,693 (GRCm39) T492K possibly damaging Het
Lgr5 T C 10: 115,314,328 (GRCm39) D203G probably damaging Het
Lpcat2 G A 8: 93,606,071 (GRCm39) V241M probably damaging Het
Mpeg1 G A 19: 12,440,361 (GRCm39) M606I probably benign Het
Ms4a1 G A 19: 11,236,176 (GRCm39) P4S probably benign Het
Nat10 A T 2: 103,587,488 (GRCm39) probably benign Het
Ncaph2 T A 15: 89,240,148 (GRCm39) probably null Het
Nfat5 T A 8: 108,078,070 (GRCm39) V403D probably damaging Het
Or2y10 T A 11: 49,455,240 (GRCm39) M164K possibly damaging Het
Or4c109 A G 2: 88,818,516 (GRCm39) F10S probably damaging Het
Pax7 T C 4: 139,557,576 (GRCm39) H65R probably damaging Het
Phf20 C A 2: 156,144,782 (GRCm39) H797N probably benign Het
Pkhd1l1 T A 15: 44,455,283 (GRCm39) S4015T probably benign Het
Prdm5 C T 6: 65,833,074 (GRCm39) H148Y probably benign Het
Prpf8 A G 11: 75,400,054 (GRCm39) probably null Het
Rangap1 A C 15: 81,594,664 (GRCm39) D388E probably benign Het
Sardh A G 2: 27,129,625 (GRCm39) probably null Het
Serpinb6b G A 13: 33,162,133 (GRCm39) E192K probably benign Het
Shfl A T 9: 20,780,212 (GRCm39) M1L probably benign Het
Skor1 A G 9: 63,053,205 (GRCm39) S216P probably damaging Het
Slc51a A G 16: 32,296,364 (GRCm39) probably null Het
Smarcb1 T C 10: 75,751,013 (GRCm39) T74A probably benign Het
Spg11 A T 2: 121,945,198 (GRCm39) Y107* probably null Het
Spmip5 A T 19: 58,774,678 (GRCm39) L176H probably damaging Het
Stk25 T A 1: 93,552,330 (GRCm39) K350M probably benign Het
Syne1 C T 10: 5,355,444 (GRCm39) W379* probably null Het
Taco1 A T 11: 105,960,437 (GRCm39) E126V probably benign Het
Tbc1d12 T A 19: 38,905,232 (GRCm39) L649* probably null Het
Trpm3 G A 19: 22,744,120 (GRCm39) G238R probably damaging Het
Yy1 C A 12: 108,759,663 (GRCm39) Q109K probably damaging Het
Other mutations in Kras
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00808:Kras APN 6 145,192,474 (GRCm39) missense probably damaging 1.00
IGL02929:Kras APN 6 145,177,815 (GRCm39) intron probably benign
N/A - 293:Kras UTSW 6 145,177,940 (GRCm39) missense probably benign 0.01
R1463:Kras UTSW 6 145,170,787 (GRCm39) intron probably benign
R1518:Kras UTSW 6 145,177,977 (GRCm39) missense probably benign 0.00
R1603:Kras UTSW 6 145,170,871 (GRCm39) nonsense probably null
R1885:Kras UTSW 6 145,177,843 (GRCm39) missense probably damaging 1.00
R5133:Kras UTSW 6 145,177,879 (GRCm39) missense probably benign 0.00
R7710:Kras UTSW 6 145,166,354 (GRCm39) missense probably benign
R7876:Kras UTSW 6 145,170,848 (GRCm39) missense probably benign
R8151:Kras UTSW 6 145,166,360 (GRCm39) small deletion probably benign
R8944:Kras UTSW 6 145,170,853 (GRCm39) missense probably benign
R8951:Kras UTSW 6 145,166,338 (GRCm39) missense probably benign
R9345:Kras UTSW 6 145,192,442 (GRCm39) missense probably benign 0.00
Z1177:Kras UTSW 6 145,192,498 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CGTTTAATACCTCAGAGCAGAAC -3'
(R):5'- TAGCTCATGTTTTAACGCTGC -3'

Sequencing Primer
(F):5'- TTTAATACCTCAGAGCAGAACCACAC -3'
(R):5'- ATGTTTTAACGCTGCATCCTC -3'
Posted On 2016-06-06