Mutagenetix > Incidental Mutation

Incidental Mutation 'R0442:Taar1'

39225

Institutional Source

Beutler Lab

Gene Symbol

Taar1

Ensembl Gene

ENSMUSG00000056379

Gene Name

trace amine-associated receptor 1

Synonyms

Trar1, Tar1

MMRRC Submission

038643-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R0442 (G1)

Quality Score

143

Status

Validated

Chromosome

Chromosomal Location

23796304-23797302 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23796380 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 26 (Y26C)

Ref Sequence

ENSEMBL: ENSMUSP00000049527 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051532]

AlphaFold

Q923Y8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000051532
AA Change: Y26C

PolyPhen 2 Score 0.801 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000049527
Gene: ENSMUSG00000056379
AA Change: Y26C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	316	1.4e-11	PFAM
Pfam:7tm_1	39	301	2.4e-62	PFAM

Meta Mutation Damage Score

0.3327

Coding Region Coverage

1x: 98.8%
3x: 97.5%
10x: 93.4%
20x: 81.2%

Validation Efficiency

99% (70/71)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a G-protein coupled receptor activated by trace amines. The encoded protein responds little or not at all to dopamine, serotonin, epinephrine, or histamine, but responds well to beta-phenylethylamine, p-tyramine, octopamine, and tryptamine. While primarily functioning in neurologic systems, there is evidence that this gene is involved in blood cell and immunologic functions as well. This gene is thought to be intronless. [provided by RefSeq, Nov 2015]
PHENOTYPE: Mice homozygous for a null mutation display decreased prepulse inhibition and increased sensitivity to amphetamines. Mice homozygous for another knock-out allele exhibit increased sensitivity to MDMA-induced hyperthermia, brain dopamine and serotonin levels, and induced hyperactivity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb3	T	A	1: 25,435,551 (GRCm39)	N816Y	probably damaging	Het
Arfgef3	T	C	10: 18,553,563 (GRCm39)		probably benign	Het
Cd200	T	A	16: 45,217,518 (GRCm39)	S58C	probably damaging	Het
Cep128	C	T	12: 91,233,545 (GRCm39)	E508K	probably damaging	Het
Dnah2	C	A	11: 69,339,368 (GRCm39)	L3046F	probably damaging	Het
Duox2	T	C	2: 122,119,813 (GRCm39)	N872D	probably benign	Het
Fam90a1a	C	T	8: 22,453,074 (GRCm39)	T143I	probably benign	Het
Fdft1	T	C	14: 63,400,798 (GRCm39)	T112A	probably benign	Het
Gimap9	G	T	6: 48,655,000 (GRCm39)	G196*	probably null	Het
Grhl1	G	A	12: 24,662,169 (GRCm39)	R536Q	probably damaging	Het
Gtpbp3	T	A	8: 71,944,135 (GRCm39)	V293E	probably damaging	Het
Hcn3	A	T	3: 89,058,847 (GRCm39)	F251Y	probably damaging	Het
Hectd4	T	A	5: 121,462,045 (GRCm39)	C971S	possibly damaging	Het
Helz2	T	A	2: 180,874,002 (GRCm39)	D2164V	probably damaging	Het
Hif1an	T	G	19: 44,554,451 (GRCm39)	L188R	probably damaging	Het
Hjurp	GT	GTT	1: 88,194,246 (GRCm39)		probably null	Het
Iqgap3	C	T	3: 88,023,266 (GRCm39)	P519L	probably damaging	Het
Jakmip1	T	G	5: 37,292,897 (GRCm39)		probably null	Het
Klra1	T	C	6: 130,349,835 (GRCm39)	Y201C	probably damaging	Het
Minpp1	T	C	19: 32,471,348 (GRCm39)	F299L	possibly damaging	Het
Myb	A	G	10: 21,002,095 (GRCm39)	S749P	probably benign	Het
Myo3b	T	C	2: 70,069,305 (GRCm39)		probably null	Het
Naip1	T	A	13: 100,581,024 (GRCm39)	R74S	probably benign	Het
Nt5m	A	G	11: 59,765,445 (GRCm39)	T158A	possibly damaging	Het
Obscn	A	T	11: 58,893,000 (GRCm39)		probably benign	Het
Or5af1	T	A	11: 58,722,257 (GRCm39)	Y92*	probably null	Het
Or5b119	T	G	19: 13,457,412 (GRCm39)	D50A	probably damaging	Het
Or6c210	T	C	10: 129,495,693 (GRCm39)	I6T	probably benign	Het
Otogl	T	A	10: 107,712,716 (GRCm39)	T543S	probably damaging	Het
Pds5b	T	C	5: 150,640,009 (GRCm39)		probably benign	Het
Plekhm1	A	G	11: 103,288,000 (GRCm39)	M49T	possibly damaging	Het
Rabl6	A	T	2: 25,477,534 (GRCm39)	S305R	probably damaging	Het
Rad54b	G	A	4: 11,609,480 (GRCm39)		probably benign	Het
Rad54b	C	A	4: 11,610,362 (GRCm39)	R660S	probably benign	Het
Rexo5	T	C	7: 119,442,508 (GRCm39)	L542P	probably damaging	Het
Rp1	C	T	1: 4,416,970 (GRCm39)	D1381N	probably benign	Het
Scnn1a	T	G	6: 125,316,100 (GRCm39)	M346R	probably damaging	Het
Sirpb1c	A	G	3: 15,856,710 (GRCm39)	I380T	probably benign	Het
Snrnp40	C	G	4: 130,271,836 (GRCm39)		probably null	Het
Sstr3	A	T	15: 78,424,597 (GRCm39)	L50Q	probably damaging	Het
St3gal6	C	A	16: 58,293,816 (GRCm39)	A238S	probably damaging	Het
St3gal6	G	T	16: 58,293,818 (GRCm39)	A237E	probably damaging	Het
Sun5	T	C	2: 153,712,872 (GRCm39)	D16G	possibly damaging	Het
Svil	A	T	18: 5,046,870 (GRCm39)	T39S	probably damaging	Het
Ugt1a1	AT	A	1: 88,140,093 (GRCm39)		probably null	Het
Use1	T	C	8: 71,819,702 (GRCm39)		probably benign	Het
Usp54	T	C	14: 20,657,277 (GRCm39)	Y7C	probably damaging	Het
Zbtb37	A	G	1: 160,859,918 (GRCm39)	F129S	possibly damaging	Het
Zfhx2	T	C	14: 55,304,357 (GRCm39)	H1209R	possibly damaging	Het
Zfp28	T	C	7: 6,397,998 (GRCm39)	L811P	probably damaging	Het
Zfp616	T	C	11: 73,975,321 (GRCm39)	I530T	possibly damaging	Het

Other mutations in Taar1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00782:Taar1	APN	10	23,796,344 (GRCm39)	missense	probably benign	0.00
R0022:Taar1	UTSW	10	23,796,625 (GRCm39)	missense	probably benign	0.45
R0126:Taar1	UTSW	10	23,796,445 (GRCm39)	missense	probably benign	0.00
R0720:Taar1	UTSW	10	23,796,971 (GRCm39)	missense	probably damaging	1.00
R0931:Taar1	UTSW	10	23,797,181 (GRCm39)	missense	probably damaging	1.00
R1270:Taar1	UTSW	10	23,796,431 (GRCm39)	missense	probably damaging	1.00
R1834:Taar1	UTSW	10	23,797,087 (GRCm39)	missense	probably benign	0.04
R2137:Taar1	UTSW	10	23,797,168 (GRCm39)	missense	probably benign
R3765:Taar1	UTSW	10	23,797,205 (GRCm39)	missense	probably damaging	1.00
R3873:Taar1	UTSW	10	23,796,482 (GRCm39)	missense	probably damaging	1.00
R5334:Taar1	UTSW	10	23,796,443 (GRCm39)	missense	probably damaging	1.00
R5418:Taar1	UTSW	10	23,797,214 (GRCm39)	missense	possibly damaging	0.91
R5578:Taar1	UTSW	10	23,796,718 (GRCm39)	missense	possibly damaging	0.71
R7048:Taar1	UTSW	10	23,796,722 (GRCm39)	missense	probably benign	0.40
R7096:Taar1	UTSW	10	23,796,809 (GRCm39)	missense	possibly damaging	0.48
R7163:Taar1	UTSW	10	23,796,918 (GRCm39)	missense	probably benign	0.38
R7707:Taar1	UTSW	10	23,797,135 (GRCm39)	missense	possibly damaging	0.69
R7854:Taar1	UTSW	10	23,796,680 (GRCm39)	missense	probably benign	0.00
R8036:Taar1	UTSW	10	23,797,033 (GRCm39)	missense	probably benign	0.01
R8442:Taar1	UTSW	10	23,796,522 (GRCm39)	nonsense	probably null
R8855:Taar1	UTSW	10	23,796,976 (GRCm39)	missense	possibly damaging	0.50
R9777:Taar1	UTSW	10	23,796,676 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCAGAATGTCCAGCTCCAACTAGG -3'
(R):5'- ACACAGCACAGTAGCGGTCAATG -3'

Sequencing Primer
(F):5'- TGAAGCATTACACGGACCTTG -3'
(R):5'- GTGAACTTTACAGAGGATTTCCCC -3'

Posted On

2013-05-23