Mutagenetix > Incidental Mutation

Incidental Mutation 'R8089:Or5h27'

629864

Institutional Source

Beutler Lab

Gene Symbol

Or5h27

Ensembl Gene

ENSMUSG00000061501

Gene Name

olfactory receptor family 5 subfamily H member 27, pseudogene 1

Synonyms

Olfr197, GA_x54KRFPKG5P-55400292-55399363, MOR183-3

MMRRC Submission

067522-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.221) question?

Stock #

R8089 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

59005919-59006844 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 59006073 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 258 (I258L)

Ref Sequence

ENSEMBL: ENSMUSP00000146916 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080031] [ENSMUST00000207772]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000080031
AA Change: I258L

Predicted Effect

unknown
Transcript: ENSMUST00000207772
AA Change: I258L

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.8%
20x: 94.7%

Validation Efficiency

93% (54/58)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,104,383 (GRCm39)	V768I	probably benign	Het
Abcc8	G	A	7: 45,757,780 (GRCm39)	T1323I	probably benign	Het
Adamtsl2	A	G	2: 26,994,809 (GRCm39)	M828V	probably benign	Het
Agmo	A	G	12: 37,397,306 (GRCm39)	D153G	probably benign	Het
Akap13	T	A	7: 75,260,340 (GRCm39)	V185D	possibly damaging	Het
Asic1	A	G	15: 99,595,968 (GRCm39)	N414D	probably damaging	Het
Bin1	T	A	18: 32,562,236 (GRCm39)		probably null	Het
Ccar1	C	A	10: 62,626,770 (GRCm39)	M1I	probably null	Het
Cdh26	T	A	2: 178,099,370 (GRCm39)		probably null	Het
Csmd3	C	T	15: 47,532,603 (GRCm39)	D1620N		Het
Dennd4a	A	T	9: 64,756,457 (GRCm39)	N204I	probably damaging	Het
Dgkb	G	A	12: 38,234,949 (GRCm39)	S438N	probably damaging	Het
Dmxl1	T	C	18: 50,021,897 (GRCm39)	M1604T	probably damaging	Het
Fhad1	T	A	4: 141,684,971 (GRCm39)	D456V	probably damaging	Het
Gcfc2	C	T	6: 81,902,771 (GRCm39)	T86M	probably damaging	Het
Idua	T	A	5: 108,829,646 (GRCm39)	M503K	probably damaging	Het
Ift70b	T	C	2: 75,767,647 (GRCm39)	T369A	possibly damaging	Het
Ighm	T	C	12: 113,384,854 (GRCm39)		probably benign	Het
Kmt2d	A	T	15: 98,740,750 (GRCm39)	S4676T	unknown	Het
Ldlrad1	G	A	4: 107,066,688 (GRCm39)	A8T	probably benign	Het
Lmtk2	G	A	5: 144,093,718 (GRCm39)	V232M	probably benign	Het
Map3k13	T	C	16: 21,722,567 (GRCm39)	V243A	possibly damaging	Het
Moxd1	A	G	10: 24,157,417 (GRCm39)	T350A	probably benign	Het
Nalcn	A	T	14: 123,537,372 (GRCm39)	W1175R	probably damaging	Het
Or10u4	A	T	10: 129,802,566 (GRCm39)	M1K	probably null	Het
Or8g26	T	A	9: 39,095,927 (GRCm39)	V148E	probably damaging	Het
Pacsin2	A	G	15: 83,263,897 (GRCm39)	I380T	probably benign	Het
Plcd1	A	C	9: 118,905,060 (GRCm39)	C214G	possibly damaging	Het
Poglut3	C	T	9: 53,307,262 (GRCm39)	A402V	probably benign	Het
Ptprm	C	A	17: 66,990,483 (GRCm39)	W1385L	possibly damaging	Het
Rab22a	C	T	2: 173,530,013 (GRCm39)	Q64*	probably null	Het
Rasa2	G	T	9: 96,435,177 (GRCm39)	H604Q	probably benign	Het
Rasal1	G	A	5: 120,809,643 (GRCm39)	G516D	probably damaging	Het
Rasgrp3	A	T	17: 75,804,056 (GRCm39)	I120L	possibly damaging	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Rgs12	T	C	5: 35,177,692 (GRCm39)	I742T	probably damaging	Het
Scyl3	A	G	1: 163,763,996 (GRCm39)	T121A	possibly damaging	Het
Six5	T	G	7: 18,828,797 (GRCm39)	F79C	probably damaging	Het
Tbx3	G	A	5: 119,818,634 (GRCm39)	R423H	probably damaging	Het
Terf2ip	C	T	8: 112,738,424 (GRCm39)	T104M	probably benign	Het
Tmem135	A	G	7: 88,805,703 (GRCm39)	C234R	probably damaging	Het
Tnxb	C	G	17: 34,891,763 (GRCm39)	A702G	unknown	Het
Tspan1	T	C	4: 116,021,532 (GRCm39)	K83R	probably null	Het
Ttn	C	T	2: 76,728,406 (GRCm39)		probably null	Het
Usp5	A	T	6: 124,797,373 (GRCm39)		probably null	Het
Vmn1r27	T	A	6: 58,192,194 (GRCm39)	Y270F	possibly damaging	Het
Vmn2r110	G	A	17: 20,803,807 (GRCm39)	T256I	probably benign	Het
Zfp831	T	A	2: 174,486,717 (GRCm39)	L464Q	possibly damaging	Het
Zscan4-ps3	C	A	7: 11,346,659 (GRCm39)	H232N	probably benign	Het

Other mutations in Or5h27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4612:Or5h27	UTSW	16	59,006,674 (GRCm39)	missense	probably damaging	1.00
R5777:Or5h27	UTSW	16	59,006,266 (GRCm39)	missense	unknown
R5945:Or5h27	UTSW	16	59,006,091 (GRCm39)	missense	unknown
R6045:Or5h27	UTSW	16	59,006,454 (GRCm39)	missense	probably benign	0.07
R6752:Or5h27	UTSW	16	59,006,694 (GRCm39)	missense	probably damaging	1.00
R7284:Or5h27	UTSW	16	59,006,331 (GRCm39)	makesense	probably null
R7327:Or5h27	UTSW	16	59,006,376 (GRCm39)	missense	unknown
R7387:Or5h27	UTSW	16	59,006,699 (GRCm39)	missense	probably damaging	1.00
R8420:Or5h27	UTSW	16	59,006,117 (GRCm39)	missense	unknown
R8489:Or5h27	UTSW	16	59,006,400 (GRCm39)	makesense	probably null
R8870:Or5h27	UTSW	16	59,006,130 (GRCm39)	missense	unknown
R9311:Or5h27	UTSW	16	59,006,106 (GRCm39)	missense	unknown
R9431:Or5h27	UTSW	16	59,006,090 (GRCm39)	missense	unknown
Z1176:Or5h27	UTSW	16	59,006,848 (GRCm39)	start gained	probably benign
Z1177:Or5h27	UTSW	16	59,006,363 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACATTAAGCCAGCACACATTTG -3'
(R):5'- CATGAAGGCATGCTGTTCCG -3'

Sequencing Primer
(F):5'- TTGACTAAAAGACAACAGATGGTAAC -3'
(R):5'- GAAGGCATGCTGTTCCGATTAACC -3'

Posted On

2020-06-30