Mutagenetix > Incidental Mutation

Incidental Mutation 'R8089:Vmn1r27'

629838

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r27

Ensembl Gene

ENSMUSG00000071428

Gene Name

vomeronasal 1 receptor 27

Synonyms

V1rc33

MMRRC Submission

067522-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R8089 (G1)

Quality Score

219.009

Status

Validated

Chromosome

Chromosomal Location

58192091-58193002 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 58192194 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 270 (Y270F)

Ref Sequence

ENSEMBL: ENSMUSP00000093547 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095862] [ENSMUST00000226666] [ENSMUST00000228530]

AlphaFold

K7N688

Predicted Effect

possibly damaging
Transcript: ENSMUST00000095862
AA Change: Y270F

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000093547
Gene: ENSMUSG00000071428
AA Change: Y270F

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	4.2e-51	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226666
AA Change: Y220F

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000228530
AA Change: Y270F

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.8%
20x: 94.7%

Validation Efficiency

93% (54/58)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,104,383 (GRCm39)	V768I	probably benign	Het
Abcc8	G	A	7: 45,757,780 (GRCm39)	T1323I	probably benign	Het
Adamtsl2	A	G	2: 26,994,809 (GRCm39)	M828V	probably benign	Het
Agmo	A	G	12: 37,397,306 (GRCm39)	D153G	probably benign	Het
Akap13	T	A	7: 75,260,340 (GRCm39)	V185D	possibly damaging	Het
Asic1	A	G	15: 99,595,968 (GRCm39)	N414D	probably damaging	Het
Bin1	T	A	18: 32,562,236 (GRCm39)		probably null	Het
Ccar1	C	A	10: 62,626,770 (GRCm39)	M1I	probably null	Het
Cdh26	T	A	2: 178,099,370 (GRCm39)		probably null	Het
Csmd3	C	T	15: 47,532,603 (GRCm39)	D1620N		Het
Dennd4a	A	T	9: 64,756,457 (GRCm39)	N204I	probably damaging	Het
Dgkb	G	A	12: 38,234,949 (GRCm39)	S438N	probably damaging	Het
Dmxl1	T	C	18: 50,021,897 (GRCm39)	M1604T	probably damaging	Het
Fhad1	T	A	4: 141,684,971 (GRCm39)	D456V	probably damaging	Het
Gcfc2	C	T	6: 81,902,771 (GRCm39)	T86M	probably damaging	Het
Idua	T	A	5: 108,829,646 (GRCm39)	M503K	probably damaging	Het
Ift70b	T	C	2: 75,767,647 (GRCm39)	T369A	possibly damaging	Het
Ighm	T	C	12: 113,384,854 (GRCm39)		probably benign	Het
Kmt2d	A	T	15: 98,740,750 (GRCm39)	S4676T	unknown	Het
Ldlrad1	G	A	4: 107,066,688 (GRCm39)	A8T	probably benign	Het
Lmtk2	G	A	5: 144,093,718 (GRCm39)	V232M	probably benign	Het
Map3k13	T	C	16: 21,722,567 (GRCm39)	V243A	possibly damaging	Het
Moxd1	A	G	10: 24,157,417 (GRCm39)	T350A	probably benign	Het
Nalcn	A	T	14: 123,537,372 (GRCm39)	W1175R	probably damaging	Het
Or10u4	A	T	10: 129,802,566 (GRCm39)	M1K	probably null	Het
Or5h27	T	G	16: 59,006,073 (GRCm39)	I258L	unknown	Het
Or8g26	T	A	9: 39,095,927 (GRCm39)	V148E	probably damaging	Het
Pacsin2	A	G	15: 83,263,897 (GRCm39)	I380T	probably benign	Het
Plcd1	A	C	9: 118,905,060 (GRCm39)	C214G	possibly damaging	Het
Poglut3	C	T	9: 53,307,262 (GRCm39)	A402V	probably benign	Het
Ptprm	C	A	17: 66,990,483 (GRCm39)	W1385L	possibly damaging	Het
Rab22a	C	T	2: 173,530,013 (GRCm39)	Q64*	probably null	Het
Rasa2	G	T	9: 96,435,177 (GRCm39)	H604Q	probably benign	Het
Rasal1	G	A	5: 120,809,643 (GRCm39)	G516D	probably damaging	Het
Rasgrp3	A	T	17: 75,804,056 (GRCm39)	I120L	possibly damaging	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Rgs12	T	C	5: 35,177,692 (GRCm39)	I742T	probably damaging	Het
Scyl3	A	G	1: 163,763,996 (GRCm39)	T121A	possibly damaging	Het
Six5	T	G	7: 18,828,797 (GRCm39)	F79C	probably damaging	Het
Tbx3	G	A	5: 119,818,634 (GRCm39)	R423H	probably damaging	Het
Terf2ip	C	T	8: 112,738,424 (GRCm39)	T104M	probably benign	Het
Tmem135	A	G	7: 88,805,703 (GRCm39)	C234R	probably damaging	Het
Tnxb	C	G	17: 34,891,763 (GRCm39)	A702G	unknown	Het
Tspan1	T	C	4: 116,021,532 (GRCm39)	K83R	probably null	Het
Ttn	C	T	2: 76,728,406 (GRCm39)		probably null	Het
Usp5	A	T	6: 124,797,373 (GRCm39)		probably null	Het
Vmn2r110	G	A	17: 20,803,807 (GRCm39)	T256I	probably benign	Het
Zfp831	T	A	2: 174,486,717 (GRCm39)	L464Q	possibly damaging	Het
Zscan4-ps3	C	A	7: 11,346,659 (GRCm39)	H232N	probably benign	Het

Other mutations in Vmn1r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Vmn1r27	APN	6	58,192,119 (GRCm39)	missense	probably benign	0.00
IGL01548:Vmn1r27	APN	6	58,192,538 (GRCm39)	missense	probably benign	0.01
IGL02662:Vmn1r27	APN	6	58,192,272 (GRCm39)	missense	probably damaging	1.00
IGL02726:Vmn1r27	APN	6	58,192,854 (GRCm39)	missense	possibly damaging	0.95
IGL02795:Vmn1r27	APN	6	58,192,287 (GRCm39)	missense	possibly damaging	0.93
IGL03241:Vmn1r27	APN	6	58,192,126 (GRCm39)	missense	probably benign	0.04
IGL03373:Vmn1r27	APN	6	58,192,689 (GRCm39)	missense	probably damaging	1.00
R0119:Vmn1r27	UTSW	6	58,192,704 (GRCm39)	missense	possibly damaging	0.56
R0124:Vmn1r27	UTSW	6	58,192,233 (GRCm39)	missense	probably damaging	1.00
R0136:Vmn1r27	UTSW	6	58,192,704 (GRCm39)	missense	possibly damaging	0.56
R3613:Vmn1r27	UTSW	6	58,192,787 (GRCm39)	missense	probably damaging	1.00
R4192:Vmn1r27	UTSW	6	58,192,812 (GRCm39)	missense	probably damaging	0.99
R4556:Vmn1r27	UTSW	6	58,192,804 (GRCm39)	missense	possibly damaging	0.94
R4831:Vmn1r27	UTSW	6	58,192,827 (GRCm39)	missense	possibly damaging	0.85
R5354:Vmn1r27	UTSW	6	58,192,581 (GRCm39)	missense	probably benign	0.00
R5813:Vmn1r27	UTSW	6	58,192,985 (GRCm39)	missense	possibly damaging	0.76
R6856:Vmn1r27	UTSW	6	58,192,432 (GRCm39)	missense	possibly damaging	0.65
R7653:Vmn1r27	UTSW	6	58,192,879 (GRCm39)	missense	probably benign	0.21
R7653:Vmn1r27	UTSW	6	58,192,785 (GRCm39)	missense	possibly damaging	0.88
R8177:Vmn1r27	UTSW	6	58,192,759 (GRCm39)	missense	probably benign	0.00
R9123:Vmn1r27	UTSW	6	58,192,416 (GRCm39)	missense	probably benign	0.00
R9125:Vmn1r27	UTSW	6	58,192,416 (GRCm39)	missense	probably benign	0.00
R9372:Vmn1r27	UTSW	6	58,192,746 (GRCm39)	missense	possibly damaging	0.95
R9422:Vmn1r27	UTSW	6	58,192,867 (GRCm39)	missense	probably benign	0.23

Predicted Primers

PCR Primer
(F):5'- AATTGGTGCTGTACAGAATGAATC -3'
(R):5'- GCATCAAGCACCTGAGAGC -3'

Sequencing Primer
(F):5'- TGCATGACATATCACCTCTGCAGG -3'
(R):5'- ACCTGAGAGCATCCCCTGAG -3'

Posted On

2020-06-30