Mutagenetix > Incidental Mutation

Incidental Mutation 'R8189:Or4f17-ps1'

635009

Institutional Source

Beutler Lab

Gene Symbol

Or4f17-ps1

Ensembl Gene

ENSMUSG00000108908

Gene Name

olfactory receptor family 4 subfamily F member 17, pseudogene 1

Synonyms

Olfr1293-ps, MOR245-24P, OTTMUSG00000015076, GA_x6K02T2Q125-72578807-72579745

MMRRC Submission

067612-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.127) question?

Stock #

R8189 (G1)

Quality Score

221.009

Status

Validated

Chromosome

Chromosomal Location

111357649-111358587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111358118 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 153 (V153A)

Ref Sequence

ENSEMBL: ENSMUSP00000099611 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102551] [ENSMUST00000120021]

AlphaFold

A0A1L1SVB5

Predicted Effect

SMART Domains

Protein: ENSMUSP00000099611
Gene: ENSMUSG00000108908
AA Change: V153A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	17	289	6e-45	PFAM
Pfam:7TM_GPCR_Srsx	20	288	1.4e-7	PFAM
Pfam:7tm_1	27	273	7e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000120021
AA Change: V156A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Meta Mutation Damage Score

0.1107

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	G	1: 71,324,885 (GRCm39)	I1531T	probably damaging	Het
Ankrd13d	G	A	19: 4,320,880 (GRCm39)	P516S	probably benign	Het
Bag6	A	G	17: 35,364,214 (GRCm39)		probably null	Het
Cchcr1	A	G	17: 35,837,563 (GRCm39)	I422V	probably benign	Het
Ceacam1	A	G	7: 25,173,343 (GRCm39)	S282P	probably damaging	Het
Chka	G	T	19: 3,925,759 (GRCm39)	E159*	probably null	Het
Ckmt2	T	C	13: 92,003,894 (GRCm39)	Y368C	probably damaging	Het
Cngb1	TTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGG	TTCTGGCTCTGGCTCTGGCTCTGG	8: 96,030,248 (GRCm39)		probably benign	Het
Ctsq	A	G	13: 61,184,969 (GRCm39)	L239P	probably damaging	Het
D630045J12Rik	A	G	6: 38,135,106 (GRCm39)	L1358P	probably damaging	Het
Dglucy	G	A	12: 100,804,889 (GRCm39)	E127K	probably benign	Het
Dnajb8	C	T	6: 88,199,940 (GRCm39)	R159C	possibly damaging	Het
Fgd6	T	C	10: 93,910,077 (GRCm39)	L794P	probably benign	Het
Fgfr2	G	A	7: 129,774,629 (GRCm39)	T654I	probably damaging	Het
Gmeb2	G	A	2: 180,919,760 (GRCm39)	T38M	probably damaging	Het
Gria1	T	C	11: 57,108,625 (GRCm39)	F243L	probably benign	Het
Gria2	A	T	3: 80,629,489 (GRCm39)	D244E	probably damaging	Het
Ints2	T	A	11: 86,106,396 (GRCm39)	I1005F	probably damaging	Het
Ipo11	T	A	13: 107,061,604 (GRCm39)	S19C	probably damaging	Het
Kmt2b	A	G	7: 30,268,756 (GRCm39)	F2658L	probably damaging	Het
Lrrc38	G	A	4: 143,077,303 (GRCm39)	G189R	probably damaging	Het
Mcf2l	A	G	8: 13,013,164 (GRCm39)	D27G	probably damaging	Het
Mrgprb2	G	T	7: 48,202,502 (GRCm39)	Y74*	probably null	Het
Mtrex	T	A	13: 113,028,515 (GRCm39)	R628S	possibly damaging	Het
Nrcam	C	A	12: 44,617,291 (GRCm39)	P740T	possibly damaging	Het
Nrxn1	C	T	17: 91,011,637 (GRCm39)	V331I	probably damaging	Het
Or2a57	T	C	6: 43,213,013 (GRCm39)	V157A	probably benign	Het
Or5k1b	T	A	16: 58,581,288 (GRCm39)	N84Y	probably damaging	Het
Or5p52	A	G	7: 107,501,939 (GRCm39)	N5S	probably damaging	Het
Or6c66b	T	A	10: 129,377,122 (GRCm39)	C239S	probably damaging	Het
Pla2g4a	A	G	1: 149,733,337 (GRCm39)	F479L	probably benign	Het
Pramel20	T	G	4: 143,298,530 (GRCm39)	Y158D	probably benign	Het
Scaper	A	T	9: 55,819,404 (GRCm39)	F104I	probably damaging	Het
Serpinb3c	A	T	1: 107,204,039 (GRCm39)	C58S	probably benign	Het
Setdb1	A	G	3: 95,254,022 (GRCm39)	V260A	probably damaging	Het
Shank3	A	G	15: 89,433,439 (GRCm39)	T1395A	probably benign	Het
Slc35b4	A	G	6: 34,144,570 (GRCm39)	I95T	probably damaging	Het
Trpm5	A	G	7: 142,635,575 (GRCm39)	V599A	probably benign	Het
Ttk	A	G	9: 83,729,272 (GRCm39)	S352G	probably benign	Het
Ugt2b35	T	A	5: 87,149,302 (GRCm39)	S184R	probably damaging	Het
Usp17le	C	T	7: 104,418,555 (GRCm39)	D196N	probably damaging	Het
Vmn2r15	A	G	5: 109,434,713 (GRCm39)	F664L	probably benign	Het
Vmn2r94	A	T	17: 18,478,618 (GRCm39)	L43Q	probably damaging	Het

Other mutations in Or4f17-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R3933:Or4f17-ps1	UTSW	2	111,358,300 (GRCm39)	missense	probably damaging	1.00
R4231:Or4f17-ps1	UTSW	2	111,358,546 (GRCm39)	missense	probably damaging	1.00
R4364:Or4f17-ps1	UTSW	2	111,357,985 (GRCm39)	missense	probably benign	0.24
R4935:Or4f17-ps1	UTSW	2	111,357,793 (GRCm39)	missense	probably damaging	1.00
R4957:Or4f17-ps1	UTSW	2	111,358,569 (GRCm39)	missense	probably benign	0.00
R4959:Or4f17-ps1	UTSW	2	111,357,969 (GRCm39)	missense	probably damaging	1.00
R4973:Or4f17-ps1	UTSW	2	111,357,969 (GRCm39)	missense	probably damaging	1.00
R5333:Or4f17-ps1	UTSW	2	111,358,048 (GRCm39)	missense	probably benign
R6087:Or4f17-ps1	UTSW	2	111,358,526 (GRCm39)	missense	possibly damaging	0.81
R6431:Or4f17-ps1	UTSW	2	111,358,001 (GRCm39)	missense	probably damaging	1.00
R8345:Or4f17-ps1	UTSW	2	111,357,864 (GRCm39)	nonsense	probably null
R9260:Or4f17-ps1	UTSW	2	111,358,271 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TGTCCATGGCTGTTTTGCAC -3'
(R):5'- CGCTGGATGGTAGTTAGAATGATACTG -3'

Sequencing Primer
(F):5'- GTTTTGCACAGATATTTCTCCTTCAC -3'
(R):5'- TGGTAGTTAGAATGATACTGTAGGAG -3'

Posted On

2020-07-13