Mutagenetix > Incidental Mutation

Incidental Mutation 'R8507:Or1j15'

655617

Institutional Source

Beutler Lab

Gene Symbol

Or1j15

Ensembl Gene

ENSMUSG00000096822

Gene Name

olfactory receptor family 1 subfamily J member 15

Synonyms

MOR136-12, Olfr344, GA_x6K02T2NLDC-33262744-33263673

MMRRC Submission

067843-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.188) question?

Stock #

R8507 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

36458612-36459541 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 36459443 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 278 (Y278H)

Ref Sequence

ENSEMBL: ENSMUSP00000151202 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075474] [ENSMUST00000215879]

AlphaFold

Q8VFP9

Predicted Effect

probably damaging
Transcript: ENSMUST00000075474
AA Change: Y278H

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000074919
Gene: ENSMUSG00000096822
AA Change: Y278H

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	5.7e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215879
AA Change: Y278H

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam7	A	G	14: 68,763,773 (GRCm39)	C126R	probably damaging	Het
Agpat5	G	A	8: 18,928,043 (GRCm39)	V203I	possibly damaging	Het
Ankrd12	T	A	17: 66,293,904 (GRCm39)	R510*	probably null	Het
Anxa6	C	T	11: 54,904,696 (GRCm39)	A22T	probably benign	Het
Barx2	A	T	9: 31,770,309 (GRCm39)	L73Q	probably damaging	Het
BC024139	T	C	15: 76,004,333 (GRCm39)	K702R	possibly damaging	Het
Cc2d1a	T	C	8: 84,861,605 (GRCm39)	K739R	probably benign	Het
Cdk12	A	G	11: 98,141,111 (GRCm39)	T1451A	unknown	Het
Chd7	A	G	4: 8,858,675 (GRCm39)	E2367G	probably damaging	Het
Cp	A	G	3: 20,025,193 (GRCm39)	Y384C	probably damaging	Het
Dnah14	T	A	1: 181,468,979 (GRCm39)	W1270R	probably benign	Het
Dyrk2	G	T	10: 118,696,567 (GRCm39)	S230R	probably damaging	Het
Epn3	A	T	11: 94,384,602 (GRCm39)	S290R	probably damaging	Het
Fas	C	T	19: 34,304,626 (GRCm39)	R296C	probably benign	Het
Fmnl1	A	T	11: 103,084,859 (GRCm39)	N659I	unknown	Het
Gas8	T	C	8: 124,257,777 (GRCm39)		probably null	Het
Gpr156	A	G	16: 37,768,598 (GRCm39)	T40A	probably benign	Het
Hey1	G	C	3: 8,729,836 (GRCm39)	A207G	probably benign	Het
Htr3b	C	A	9: 48,876,177 (GRCm39)		probably benign	Het
Itgal	C	T	7: 126,928,607 (GRCm39)	T1044I	probably benign	Het
Itprid2	A	G	2: 79,475,208 (GRCm39)	Q389R	probably benign	Het
Kcnip4	T	G	5: 48,639,997 (GRCm39)	D38A	possibly damaging	Het
Kcnma1	G	T	14: 23,641,706 (GRCm39)	Q216K	probably benign	Het
Kdsr	T	A	1: 106,671,400 (GRCm39)	E203V	probably null	Het
Lrp1b	T	C	2: 41,298,387 (GRCm39)	E999G		Het
Malt1	T	A	18: 65,603,594 (GRCm39)	W577R	probably damaging	Het
Mroh2b	A	C	15: 4,978,572 (GRCm39)	T1373P	probably damaging	Het
Mymk	A	G	2: 26,952,712 (GRCm39)		probably null	Het
Myo1f	T	A	17: 33,816,992 (GRCm39)	H707Q	probably benign	Het
Ncam2	C	A	16: 81,309,867 (GRCm39)	H452Q	possibly damaging	Het
Ndrg4	A	G	8: 96,404,975 (GRCm39)	M1V	probably null	Het
Nps	C	T	7: 134,874,079 (GRCm39)	S83L	probably damaging	Het
Nup155	T	A	15: 8,177,044 (GRCm39)	Y1040*	probably null	Het
Or2ak4	C	T	11: 58,648,985 (GRCm39)	Q165*	probably null	Het
Or4f7	T	C	2: 111,645,051 (GRCm39)	T7A	probably benign	Het
Or5h22	C	T	16: 58,895,243 (GRCm39)	V67M	possibly damaging	Het
Pak1ip1	G	A	13: 41,162,770 (GRCm39)	R191Q	probably benign	Het
Pcdh17	G	A	14: 84,683,384 (GRCm39)		probably benign	Het
Pcdhgc5	G	T	18: 37,952,945 (GRCm39)	R73L	probably benign	Het
Peg10	C	CTCA	6: 4,756,453 (GRCm39)		probably benign	Het
Plat	G	T	8: 23,262,248 (GRCm39)	G91W	probably damaging	Het
Plscr4	C	T	9: 92,372,843 (GRCm39)	R322*	probably null	Het
Plxnd1	A	T	6: 115,943,866 (GRCm39)	N1144K	probably damaging	Het
Ppp1r1b	G	T	11: 98,246,310 (GRCm39)	E133D	probably damaging	Het
Ptpn13	A	G	5: 103,705,815 (GRCm39)	E1396G	probably damaging	Het
Reps1	T	C	10: 17,970,218 (GRCm39)	S272P	probably damaging	Het
Ric8b	T	C	10: 84,816,039 (GRCm39)	V430A	probably damaging	Het
Septin10	A	T	10: 59,012,825 (GRCm39)	N264K	possibly damaging	Het
Sgms1	A	T	19: 32,137,109 (GRCm39)	F152L	probably benign	Het
Snapc1	C	A	12: 74,011,506 (GRCm39)	F57L	probably damaging	Het
Spidr	A	G	16: 15,786,540 (GRCm39)	L401P	probably damaging	Het
Spn	C	T	7: 126,735,728 (GRCm39)	V260M	probably damaging	Het
Sptan1	G	A	2: 29,916,596 (GRCm39)	A2095T	probably damaging	Het
Tbc1d15	A	G	10: 115,038,407 (GRCm39)		probably null	Het
Thsd7b	T	A	1: 129,605,790 (GRCm39)	F510L	probably benign	Het
Trpm5	T	A	7: 142,632,050 (GRCm39)	I920F	probably damaging	Het
Tspear	C	A	10: 77,710,898 (GRCm39)	H507N	probably benign	Het
Vmn2r108	T	A	17: 20,683,195 (GRCm39)	K670*	probably null	Het
Vmn2r75	A	T	7: 85,797,685 (GRCm39)	C709*	probably null	Het
Wdfy3	G	T	5: 102,020,767 (GRCm39)	S2494R	probably benign	Het
Zfp456	A	T	13: 67,515,108 (GRCm39)	F199L	probably damaging	Het
Zfp830	A	G	11: 82,655,529 (GRCm39)	Q111R	probably benign	Het
Znrf1	G	A	8: 112,263,842 (GRCm39)	A24T	probably damaging	Het

Other mutations in Or1j15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or1j15	APN	2	36,458,838 (GRCm39)	missense	probably damaging	1.00
IGL01450:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01452:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01458:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01466:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01470:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01476:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01477:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01478:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01480:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01481:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01487:Or1j15	APN	2	36,458,754 (GRCm39)	missense	probably damaging	1.00
IGL01522:Or1j15	APN	2	36,459,233 (GRCm39)	missense	probably benign	0.00
IGL02141:Or1j15	APN	2	36,458,820 (GRCm39)	missense	probably damaging	1.00
IGL02510:Or1j15	APN	2	36,458,693 (GRCm39)	missense	possibly damaging	0.87
IGL02896:Or1j15	APN	2	36,459,217 (GRCm39)	missense	possibly damaging	0.88
IGL03032:Or1j15	APN	2	36,458,716 (GRCm39)	nonsense	probably null
R0081:Or1j15	UTSW	2	36,458,893 (GRCm39)	nonsense	probably null
R0581:Or1j15	UTSW	2	36,458,834 (GRCm39)	missense	probably damaging	1.00
R0611:Or1j15	UTSW	2	36,459,568 (GRCm39)	splice site	probably null
R1503:Or1j15	UTSW	2	36,458,885 (GRCm39)	missense	probably damaging	1.00
R1844:Or1j15	UTSW	2	36,458,789 (GRCm39)	missense	probably damaging	1.00
R2320:Or1j15	UTSW	2	36,458,637 (GRCm39)	missense	possibly damaging	0.90
R4088:Or1j15	UTSW	2	36,459,030 (GRCm39)	missense	probably damaging	1.00
R5243:Or1j15	UTSW	2	36,458,655 (GRCm39)	missense	probably damaging	1.00
R5747:Or1j15	UTSW	2	36,458,979 (GRCm39)	missense	probably damaging	0.98
R5948:Or1j15	UTSW	2	36,459,363 (GRCm39)	missense	probably damaging	1.00
R6115:Or1j15	UTSW	2	36,458,963 (GRCm39)	missense	probably damaging	1.00
R6158:Or1j15	UTSW	2	36,459,128 (GRCm39)	missense	probably benign	0.03
R6198:Or1j15	UTSW	2	36,458,963 (GRCm39)	missense	probably damaging	1.00
R6531:Or1j15	UTSW	2	36,459,353 (GRCm39)	missense	probably damaging	1.00
R7075:Or1j15	UTSW	2	36,459,192 (GRCm39)	missense	probably benign	0.01
R7193:Or1j15	UTSW	2	36,459,248 (GRCm39)	missense	probably benign	0.06
R7329:Or1j15	UTSW	2	36,458,708 (GRCm39)	missense	probably benign
R7659:Or1j15	UTSW	2	36,458,637 (GRCm39)	missense	possibly damaging	0.90
R8251:Or1j15	UTSW	2	36,459,467 (GRCm39)	missense	probably damaging	1.00
R8383:Or1j15	UTSW	2	36,459,014 (GRCm39)	missense	probably benign	0.08
R8698:Or1j15	UTSW	2	36,458,915 (GRCm39)	missense	possibly damaging	0.78
R8837:Or1j15	UTSW	2	36,458,703 (GRCm39)	missense	probably benign	0.35
R9087:Or1j15	UTSW	2	36,459,345 (GRCm39)	missense	probably damaging	1.00
R9149:Or1j15	UTSW	2	36,458,988 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- TATGGCTACATTGGTGTCACC -3'
(R):5'- GTGTACAGTCCAAGTTATATGCAAG -3'

Sequencing Primer
(F):5'- GGTGTCACCATTCTGAAAACTC -3'
(R):5'- CAGTCCAAGTTATATGCAAGAAGTTC -3'

Posted On

2020-10-20