Mutagenetix > Incidental Mutation

Incidental Mutation 'R8869:Cyp4f37'

676162

Institutional Source

Beutler Lab

Gene Symbol

Cyp4f37

Ensembl Gene

ENSMUSG00000062464

Gene Name

cytochrome P450, family 4, subfamily f, polypeptide 37

Synonyms

Gm9705

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.222) question?

Stock #

R8869 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

32840283-32855158 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 32844096 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 67 (I67F)

Ref Sequence

ENSEMBL: ENSMUSP00000076827 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077639]

AlphaFold

Q3V1F1

Predicted Effect

probably benign
Transcript: ENSMUST00000077639
AA Change: I67F

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000076827
Gene: ENSMUSG00000062464
AA Change: I67F

Domain	Start	End	E-Value	Type
Pfam:p450	52	515	1.1e-136	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsl5	G	A	19: 55,266,523 (GRCm39)	R114Q	possibly damaging	Het
Atp1a4	C	T	1: 172,054,690 (GRCm39)	V980I	probably benign	Het
Bltp1	T	A	3: 37,013,007 (GRCm39)	C1895S	probably damaging	Het
Brd1	A	T	15: 88,614,729 (GRCm39)	D55E	probably benign	Het
Cachd1	A	G	4: 100,809,280 (GRCm39)	D255G	probably benign	Het
Ccdc168	A	T	1: 44,097,425 (GRCm39)	D1224E	possibly damaging	Het
Ces2f	C	A	8: 105,676,704 (GRCm39)	P133T	probably damaging	Het
Cfap251	A	T	5: 123,460,505 (GRCm39)	M1156L	possibly damaging	Het
Clock	GGCTGCTGCTGCTGCTGCTGCTGCTG	GGCTGCTGCTGCTGCTGCTGCTG	5: 76,374,889 (GRCm39)		probably benign	Het
Dbf4	T	C	5: 8,448,656 (GRCm39)	I84V		Het
Dcc	T	C	18: 71,511,755 (GRCm39)	T887A	probably benign	Het
Dhdh	G	T	7: 45,137,536 (GRCm39)	N50K	probably benign	Het
Dnah7c	A	T	1: 46,671,504 (GRCm39)	E1631V	probably damaging	Het
Dusp23	A	G	1: 172,460,293 (GRCm39)	C53R	possibly damaging	Het
Efcab6	G	A	15: 83,928,432 (GRCm39)	S54L	probably damaging	Het
Fcamr	T	C	1: 130,739,335 (GRCm39)	F212L	probably damaging	Het
Gfap	G	C	11: 102,787,810 (GRCm39)	A45G	probably benign	Het
Grm8	C	T	6: 27,363,752 (GRCm39)	V588M	probably benign	Het
H1f8	A	G	6: 115,926,911 (GRCm39)	T263A	probably benign	Het
Hoatz	T	A	9: 50,992,832 (GRCm39)		probably null	Het
Inhca	T	C	9: 103,149,831 (GRCm39)	Y242C	probably damaging	Het
Malrd1	T	A	2: 15,570,368 (GRCm39)		probably null	Het
Mxi1	G	A	19: 53,360,126 (GRCm39)	G283S	probably damaging	Het
Myh15	T	C	16: 48,997,366 (GRCm39)	V1728A	probably benign	Het
Nxph2	T	A	2: 23,290,071 (GRCm39)	V141D	probably damaging	Het
Or11l3	T	C	11: 58,515,994 (GRCm39)	E106G	unknown	Het
Or2t26	A	T	11: 49,039,483 (GRCm39)	Y133F	probably damaging	Het
Or4f6	A	T	2: 111,838,596 (GRCm39)	S312T	possibly damaging	Het
Or5b121	A	T	19: 13,507,892 (GRCm39)	N329I	probably damaging	Het
Or5d36	T	A	2: 87,901,753 (GRCm39)		probably null	Het
Or5h25	A	G	16: 58,930,121 (GRCm39)	V284A		Het
Or5w10	A	T	2: 87,375,753 (GRCm39)	M45K	probably damaging	Het
Or8c10	A	G	9: 38,279,142 (GRCm39)	N90S	possibly damaging	Het
Otof	T	A	5: 30,578,325 (GRCm39)	I108F	probably benign	Het
Pcdha4	A	T	18: 37,086,011 (GRCm39)	R65*	probably null	Het
Pde11a	T	C	2: 76,041,434 (GRCm39)	H412R	probably benign	Het
Peli3	C	T	19: 4,982,541 (GRCm39)	G375S	probably damaging	Het
Ppp6r3	A	G	19: 3,561,927 (GRCm39)		probably null	Het
Prcp	T	C	7: 92,559,518 (GRCm39)	V194A	possibly damaging	Het
Prkce	T	C	17: 86,476,370 (GRCm39)		probably null	Het
Prss23	A	T	7: 89,159,887 (GRCm39)	S61T	probably benign	Het
Ptprq	C	T	10: 107,535,469 (GRCm39)	R432H	probably damaging	Het
Rnase2a	T	A	14: 51,493,101 (GRCm39)	N88I	possibly damaging	Het
Rspry1	G	T	8: 95,359,780 (GRCm39)	L230F	probably damaging	Het
Slco1a8	A	G	6: 141,927,810 (GRCm39)	V548A	probably damaging	Het
Smpd2	A	G	10: 41,365,301 (GRCm39)	L65P	probably benign	Het
Sorl1	A	G	9: 41,933,722 (GRCm39)	Y1083H	probably benign	Het
Tjp1	A	G	7: 64,986,386 (GRCm39)	S241P	probably damaging	Het
Tmed6	A	C	8: 107,792,164 (GRCm39)	L27R	probably damaging	Het
Tmem52	G	A	4: 155,553,788 (GRCm39)	C32Y	probably damaging	Het
Tmprss15	C	A	16: 78,750,834 (GRCm39)	G1022*	probably null	Het
Ttn	T	C	2: 76,730,345 (GRCm39)	S5085G	unknown	Het
Vmn2r37	C	T	7: 9,209,854 (GRCm39)	V553M	possibly damaging	Het
Wdr70	A	T	15: 8,123,210 (GRCm39)	M42K	probably benign	Het
Zfp735	T	A	11: 73,602,510 (GRCm39)	C485S	possibly damaging	Het

Other mutations in Cyp4f37

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Cyp4f37	APN	17	32,848,027 (GRCm39)	missense	probably benign	0.20
IGL01994:Cyp4f37	APN	17	32,844,150 (GRCm39)	nonsense	probably null
IGL02073:Cyp4f37	APN	17	32,846,825 (GRCm39)	missense	possibly damaging	0.59
IGL02145:Cyp4f37	APN	17	32,849,009 (GRCm39)	missense	probably benign	0.43
IGL02814:Cyp4f37	APN	17	32,853,645 (GRCm39)	missense	probably benign	0.01
IGL02873:Cyp4f37	APN	17	32,844,142 (GRCm39)	missense	probably benign	0.00
IGL02937:Cyp4f37	APN	17	32,844,163 (GRCm39)	missense	probably benign	0.00
IGL03170:Cyp4f37	APN	17	32,844,093 (GRCm39)	splice site	probably benign
R0625:Cyp4f37	UTSW	17	32,853,652 (GRCm39)	missense	probably damaging	1.00
R1774:Cyp4f37	UTSW	17	32,848,864 (GRCm39)	missense	possibly damaging	0.59
R1871:Cyp4f37	UTSW	17	32,853,639 (GRCm39)	missense	probably damaging	1.00
R2232:Cyp4f37	UTSW	17	32,853,244 (GRCm39)	missense	probably benign	0.23
R2847:Cyp4f37	UTSW	17	32,848,099 (GRCm39)	missense	probably damaging	1.00
R2848:Cyp4f37	UTSW	17	32,848,099 (GRCm39)	missense	probably damaging	1.00
R4027:Cyp4f37	UTSW	17	32,850,646 (GRCm39)	missense	probably benign	0.00
R4463:Cyp4f37	UTSW	17	32,846,710 (GRCm39)	critical splice acceptor site	probably null
R4517:Cyp4f37	UTSW	17	32,850,566 (GRCm39)	missense	probably benign	0.00
R4573:Cyp4f37	UTSW	17	32,848,061 (GRCm39)	missense	probably benign	0.23
R4670:Cyp4f37	UTSW	17	32,844,126 (GRCm39)	missense	probably benign
R5752:Cyp4f37	UTSW	17	32,850,306 (GRCm39)	missense	probably damaging	1.00
R5930:Cyp4f37	UTSW	17	32,848,957 (GRCm39)	missense	possibly damaging	0.79
R6248:Cyp4f37	UTSW	17	32,848,864 (GRCm39)	missense	possibly damaging	0.59
R7412:Cyp4f37	UTSW	17	32,848,818 (GRCm39)	missense	possibly damaging	0.87
R7969:Cyp4f37	UTSW	17	32,844,181 (GRCm39)	missense	probably benign	0.00
R8066:Cyp4f37	UTSW	17	32,854,047 (GRCm39)	missense	probably benign	0.21
R8187:Cyp4f37	UTSW	17	32,854,171 (GRCm39)	missense	probably benign
R8303:Cyp4f37	UTSW	17	32,853,152 (GRCm39)	critical splice acceptor site	probably null
R8309:Cyp4f37	UTSW	17	32,853,952 (GRCm39)	missense	probably damaging	1.00
R8407:Cyp4f37	UTSW	17	32,853,158 (GRCm39)	missense	probably damaging	1.00
R9054:Cyp4f37	UTSW	17	32,853,253 (GRCm39)	missense	probably benign	0.25
R9565:Cyp4f37	UTSW	17	32,844,205 (GRCm39)	missense	possibly damaging	0.79
R9674:Cyp4f37	UTSW	17	32,846,841 (GRCm39)	critical splice donor site	probably null
X0057:Cyp4f37	UTSW	17	32,844,198 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GAGCGACATGGATAGACTCATG -3'
(R):5'- TGGTCAATCCCGGGATAGAGTG -3'

Sequencing Primer
(F):5'- CGACATGGATAGACTCATGTGTAC -3'
(R):5'- TCCCGGGATAGAGTGAGCTG -3'

Posted On

2021-07-15