Mutagenetix > Incidental Mutations

Incidental Mutation 'R0850:Fbxw25'

82523

Institutional Source

Beutler Lab

Gene Symbol

Fbxw25

Ensembl Gene

ENSMUSG00000094992

Gene Name

F-box and WD-40 domain protein 25

Synonyms

E330001B16Rik

MMRRC Submission

039029-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R0850 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109645122-109664652 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109649617 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 425 (K425R)

Ref Sequence

ENSEMBL: ENSMUSP00000128652 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163839]

AlphaFold

F7C9P2

Predicted Effect

probably benign
Transcript: ENSMUST00000163839
AA Change: K425R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000128652
Gene: ENSMUSG00000094992
AA Change: K425R

Domain	Start	End	E-Value	Type
FBOX	5	45	5.44e-6	SMART
SCOP:d1gxra_	119	228	1e-6	SMART
Blast:WD40	137	176	6e-6	BLAST

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 97.8%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700016C15Rik	A	G	1: 177,741,005	T23A	probably benign	Het
4930433I11Rik	A	T	7: 40,993,056	T141S	probably benign	Het
9330182L06Rik	T	A	5: 9,417,993	N220K	probably damaging	Het
Agbl3	T	A	6: 34,799,204	F210Y	probably damaging	Het
Dgkq	A	G	5: 108,654,578	V418A	possibly damaging	Het
Dmp1	A	T	5: 104,212,787	D443V	possibly damaging	Het
Elavl3	T	G	9: 22,036,763	D35A	probably damaging	Het
Fbxo44	C	G	4: 148,156,269	R220S	probably damaging	Het
Gm5538	T	G	3: 59,752,248	I374R	possibly damaging	Het
Gypa	A	G	8: 80,496,345	H26R	unknown	Het
H2-DMb1	T	C	17: 34,155,562	V62A	probably benign	Het
Helb	T	C	10: 120,105,367	H472R	probably damaging	Het
Herc1	T	G	9: 66,466,670	V3197G	probably damaging	Het
Herc2	C	A	7: 56,204,483	N3712K	probably benign	Het
Herc6	T	C	6: 57,583,242	V89A	possibly damaging	Het
Hspa1l	A	G	17: 34,977,623	T213A	probably benign	Het
Kcna7	T	C	7: 45,409,431	S381P	probably damaging	Het
Kif19a	C	A	11: 114,780,787	P164Q	probably damaging	Het
Macf1	A	G	4: 123,474,402	S2189P	probably benign	Het
Mpo	A	G	11: 87,797,502	N329S	probably damaging	Het
Mrps15	A	G	4: 126,048,686	Y76C	probably damaging	Het
Olfr643	T	A	7: 104,059,045	M186L	probably benign	Het
Olfr790	T	C	10: 129,501,724	V280A	probably damaging	Het
Prdm2	T	C	4: 143,132,203	R1506G	possibly damaging	Het
Ptprb	A	T	10: 116,302,125	Q311H	possibly damaging	Het
Ptprb	T	C	10: 116,339,510	Y1137H	probably damaging	Het
Scaf8	A	G	17: 3,195,774		probably null	Het
Slc25a1	A	T	16: 17,927,281	F105Y	probably benign	Het
Slc29a4	T	C	5: 142,718,572	V327A	probably benign	Het
Tmed10	A	G	12: 85,343,505	F195L	probably benign	Het
Tmem45a2	T	A	16: 57,045,369	I151F	probably benign	Het
Vmn2r93	C	A	17: 18,305,017	F312L	possibly damaging	Het
Zfp326	A	G	5: 105,878,797		probably null	Het

Other mutations in Fbxw25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03330:Fbxw25	APN	9	109645239	missense	probably benign	0.00
goodtimes	UTSW	9	109663374	critical splice donor site	probably null
shakeys	UTSW	9	109654583	missense
R0158:Fbxw25	UTSW	9	109654652	missense	possibly damaging	0.74
R1109:Fbxw25	UTSW	9	109650060	missense	probably benign	0.00
R1386:Fbxw25	UTSW	9	109654641	missense	possibly damaging	0.77
R1609:Fbxw25	UTSW	9	109663510	missense	probably benign	0.11
R1750:Fbxw25	UTSW	9	109650073	missense	probably benign	0.23
R1977:Fbxw25	UTSW	9	109652856	missense	possibly damaging	0.72
R2427:Fbxw25	UTSW	9	109652860	missense	probably benign	0.09
R3841:Fbxw25	UTSW	9	109662134	nonsense	probably null
R4356:Fbxw25	UTSW	9	109662085	missense	probably damaging	1.00
R4934:Fbxw25	UTSW	9	109651637	missense	possibly damaging	0.63
R5024:Fbxw25	UTSW	9	109663374	critical splice donor site	probably null
R5175:Fbxw25	UTSW	9	109664563	missense	probably damaging	1.00
R5323:Fbxw25	UTSW	9	109663505	missense	probably benign	0.04
R5389:Fbxw25	UTSW	9	109652886	missense	possibly damaging	0.95
R5493:Fbxw25	UTSW	9	109652916	missense	probably benign	0.01
R6268:Fbxw25	UTSW	9	109654650	missense	probably damaging	1.00
R6739:Fbxw25	UTSW	9	109651631	missense	probably benign	0.29
R7275:Fbxw25	UTSW	9	109654592	missense
R7492:Fbxw25	UTSW	9	109664530	critical splice donor site	probably null
R7623:Fbxw25	UTSW	9	109654583	missense
R7784:Fbxw25	UTSW	9	109650119	missense
R7861:Fbxw25	UTSW	9	109664557	nonsense	probably null
R7887:Fbxw25	UTSW	9	109649594	critical splice donor site	probably null
R8973:Fbxw25	UTSW	9	109650064	missense
X0023:Fbxw25	UTSW	9	109651775	missense	possibly damaging	0.94
Z1176:Fbxw25	UTSW	9	109651738	missense

Predicted Primers

PCR Primer
(F):5'- TGTGCTGAGATCCCAAGAACTAGCAA -3'
(R):5'- GTCTGACATTGACTCCAGCCTACTAACA -3'

Sequencing Primer
(F):5'- ggagtgggtgggttggg -3'
(R):5'- TCGTCACCTGTAACAATGGC -3'

Posted On

2013-11-08