Incidental Mutation 'R3700:Eif3f'
ID269957
Institutional Source Beutler Lab
Gene Symbol Eif3f
Ensembl Gene ENSMUSG00000031029
Gene Nameeukaryotic translation initiation factor 3, subunit F
SynonymsEif3s5, 0610037M02Rik
MMRRC Submission 040693-MU
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.963) question?
Stock #R3700 (G1)
Quality Score225
Status Validated
Chromosome7
Chromosomal Location108934417-108942951 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 108940275 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 251 (I251F)
Ref Sequence ENSEMBL: ENSMUSP00000033342 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033342]
Predicted Effect probably benign
Transcript: ENSMUST00000033342
AA Change: I251F

PolyPhen 2 Score 0.227 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000033342
Gene: ENSMUSG00000031029
AA Change: I251F

DomainStartEndE-ValueType
low complexity region 2 93 N/A INTRINSIC
JAB_MPN 95 225 3.11e-42 SMART
Pfam:MitMem_reg 247 359 7e-27 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000147369
Predicted Effect noncoding transcript
Transcript: ENSMUST00000153596
Meta Mutation Damage Score 0.2615 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 94.2%
Validation Efficiency 98% (51/52)
Allele List at MGI
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alpk2 G A 18: 65,305,151 T1524I probably damaging Het
Ankrd29 G A 18: 12,254,700 A275V possibly damaging Het
Arhgap26 T A 18: 39,120,184 F221L probably damaging Het
Arhgap28 T C 17: 67,901,366 S36G probably damaging Het
Bcl11a A G 11: 24,163,890 D411G probably damaging Het
Cdh23 C A 10: 60,327,370 probably null Het
Cnnm2 T A 19: 46,762,551 I260N probably damaging Het
Coro6 T C 11: 77,467,303 F238S probably damaging Het
Ddah1 G A 3: 145,891,495 M162I probably benign Het
Dnah5 T C 15: 28,387,791 I3132T possibly damaging Het
Ephx4 T C 5: 107,402,807 F11L probably benign Het
Esp1 T C 17: 40,731,107 S97P unknown Het
Fbxw10 A T 11: 62,869,157 probably null Het
Hsp90ab1 T C 17: 45,571,514 T85A possibly damaging Het
Idh2 T C 7: 80,099,147 K129E probably damaging Het
Kcnj6 A G 16: 94,833,006 I64T probably damaging Het
Klk5 T A 7: 43,850,827 C280S probably damaging Het
Lhx2 T A 2: 38,360,099 L269H probably damaging Het
Lrrc71 T A 3: 87,745,878 probably null Het
Lss T A 10: 76,546,192 L484Q probably damaging Het
Mmp14 G A 14: 54,431,932 probably benign Het
Muc5b A G 7: 141,847,249 T534A unknown Het
Mysm1 T C 4: 94,970,652 K87E probably benign Het
Olfr213 T A 6: 116,540,528 V25E probably benign Het
Olfr523 C G 7: 140,176,214 F37L possibly damaging Het
Pcolce T C 5: 137,609,047 T61A probably damaging Het
Phc3 C A 3: 30,914,128 D920Y probably damaging Het
Pi4kb T C 3: 94,994,288 I422T probably benign Het
Piezo1 C T 8: 122,494,903 R584H probably damaging Het
Plce1 T A 19: 38,705,337 F768Y probably damaging Het
Ppp1r21 T C 17: 88,582,454 S709P possibly damaging Het
Prdx6 T C 1: 161,247,288 D74G probably damaging Het
Prrxl1 A T 14: 32,628,861 E218V probably damaging Het
Rlf A G 4: 121,150,863 F307L possibly damaging Het
Skiv2l T C 17: 34,849,903 E40G probably benign Het
Snapin G A 3: 90,490,192 R91* probably null Het
Sort1 T A 3: 108,356,639 L838* probably null Het
Sstr4 A G 2: 148,396,353 I295V possibly damaging Het
Stk39 T A 2: 68,392,118 I201F probably damaging Het
Tasp1 T C 2: 139,910,554 T322A probably benign Het
Tepsin C T 11: 120,091,753 C491Y possibly damaging Het
Tlr9 G A 9: 106,224,079 V190M probably damaging Het
Ttf2 A G 3: 100,951,008 L755P probably damaging Het
Txn2 G A 15: 77,927,776 T60M possibly damaging Het
Vmn1r10 A G 6: 57,114,302 N293S probably benign Het
Vmn1r232 A G 17: 20,914,203 L45P probably benign Het
Vmn2r111 C T 17: 22,571,161 W288* probably null Het
Vmn2r118 T C 17: 55,608,421 S510G possibly damaging Het
Wdr60 C T 12: 116,211,842 W905* probably null Het
Zgpat T A 2: 181,365,646 probably benign Het
Zzef1 G T 11: 72,886,772 G1810C probably null Het
Other mutations in Eif3f
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02690:Eif3f APN 7 108934718 missense probably damaging 1.00
IGL02821:Eif3f APN 7 108934674 unclassified probably benign
IGL02821:Eif3f APN 7 108934675 unclassified probably benign
R0010:Eif3f UTSW 7 108941005 missense possibly damaging 0.93
R1054:Eif3f UTSW 7 108937817 critical splice donor site probably null
R1886:Eif3f UTSW 7 108940751 missense probably benign 0.08
R2877:Eif3f UTSW 7 108934812 splice site probably null
R3882:Eif3f UTSW 7 108940955 missense possibly damaging 0.88
R4471:Eif3f UTSW 7 108940946 missense possibly damaging 0.79
R4472:Eif3f UTSW 7 108940946 missense possibly damaging 0.79
R4817:Eif3f UTSW 7 108937775 missense probably damaging 0.99
R4953:Eif3f UTSW 7 108934640 unclassified probably benign
R5744:Eif3f UTSW 7 108938417 missense probably damaging 0.99
R7162:Eif3f UTSW 7 108940731 missense probably benign 0.02
R7235:Eif3f UTSW 7 108938088 missense possibly damaging 0.78
R7445:Eif3f UTSW 7 108934658 missense unknown
Predicted Primers PCR Primer
(F):5'- GGCCTTCTCAAGAGGAAGAC -3'
(R):5'- GCTAACTATTGGTTCTGGCCTC -3'

Sequencing Primer
(F):5'- GGAAGACACTAACTTTGACTGC -3'
(R):5'- CTCATATGGATGACTATATGGCCCG -3'
Posted On2015-03-18