Mutagenetix > Incidental Mutations

Incidental Mutation 'R3700:Eif3f'

269957

Institutional Source

Beutler Lab

Gene Symbol

Eif3f

Ensembl Gene

ENSMUSG00000031029

Gene Name

eukaryotic translation initiation factor 3, subunit F

Synonyms

Eif3s5, 0610037M02Rik

MMRRC Submission

040693-MU

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.963) question?

Stock #

R3700 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108934417-108942951 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 108940275 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 251 (I251F)

Ref Sequence

ENSEMBL: ENSMUSP00000033342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033342]

Predicted Effect

probably benign
Transcript: ENSMUST00000033342
AA Change: I251F

PolyPhen 2 Score 0.227 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000033342
Gene: ENSMUSG00000031029
AA Change: I251F

Domain	Start	End	E-Value	Type
low complexity region	2	93	N/A	INTRINSIC
JAB_MPN	95	225	3.11e-42	SMART
Pfam:MitMem_reg	247	359	7e-27	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147369

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153596

Meta Mutation Damage Score

0.2615

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.2%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alpk2	G	A	18: 65,305,151	T1524I	probably damaging	Het
Ankrd29	G	A	18: 12,254,700	A275V	possibly damaging	Het
Arhgap26	T	A	18: 39,120,184	F221L	probably damaging	Het
Arhgap28	T	C	17: 67,901,366	S36G	probably damaging	Het
Bcl11a	A	G	11: 24,163,890	D411G	probably damaging	Het
Cdh23	C	A	10: 60,327,370		probably null	Het
Cnnm2	T	A	19: 46,762,551	I260N	probably damaging	Het
Coro6	T	C	11: 77,467,303	F238S	probably damaging	Het
Ddah1	G	A	3: 145,891,495	M162I	probably benign	Het
Dnah5	T	C	15: 28,387,791	I3132T	possibly damaging	Het
Ephx4	T	C	5: 107,402,807	F11L	probably benign	Het
Esp1	T	C	17: 40,731,107	S97P	unknown	Het
Fbxw10	A	T	11: 62,869,157		probably null	Het
Hsp90ab1	T	C	17: 45,571,514	T85A	possibly damaging	Het
Idh2	T	C	7: 80,099,147	K129E	probably damaging	Het
Kcnj6	A	G	16: 94,833,006	I64T	probably damaging	Het
Klk5	T	A	7: 43,850,827	C280S	probably damaging	Het
Lhx2	T	A	2: 38,360,099	L269H	probably damaging	Het
Lrrc71	T	A	3: 87,745,878		probably null	Het
Lss	T	A	10: 76,546,192	L484Q	probably damaging	Het
Mmp14	G	A	14: 54,431,932		probably benign	Het
Muc5b	A	G	7: 141,847,249	T534A	unknown	Het
Mysm1	T	C	4: 94,970,652	K87E	probably benign	Het
Olfr213	T	A	6: 116,540,528	V25E	probably benign	Het
Olfr523	C	G	7: 140,176,214	F37L	possibly damaging	Het
Pcolce	T	C	5: 137,609,047	T61A	probably damaging	Het
Phc3	C	A	3: 30,914,128	D920Y	probably damaging	Het
Pi4kb	T	C	3: 94,994,288	I422T	probably benign	Het
Piezo1	C	T	8: 122,494,903	R584H	probably damaging	Het
Plce1	T	A	19: 38,705,337	F768Y	probably damaging	Het
Ppp1r21	T	C	17: 88,582,454	S709P	possibly damaging	Het
Prdx6	T	C	1: 161,247,288	D74G	probably damaging	Het
Prrxl1	A	T	14: 32,628,861	E218V	probably damaging	Het
Rlf	A	G	4: 121,150,863	F307L	possibly damaging	Het
Skiv2l	T	C	17: 34,849,903	E40G	probably benign	Het
Snapin	G	A	3: 90,490,192	R91*	probably null	Het
Sort1	T	A	3: 108,356,639	L838*	probably null	Het
Sstr4	A	G	2: 148,396,353	I295V	possibly damaging	Het
Stk39	T	A	2: 68,392,118	I201F	probably damaging	Het
Tasp1	T	C	2: 139,910,554	T322A	probably benign	Het
Tepsin	C	T	11: 120,091,753	C491Y	possibly damaging	Het
Tlr9	G	A	9: 106,224,079	V190M	probably damaging	Het
Ttf2	A	G	3: 100,951,008	L755P	probably damaging	Het
Txn2	G	A	15: 77,927,776	T60M	possibly damaging	Het
Vmn1r10	A	G	6: 57,114,302	N293S	probably benign	Het
Vmn1r232	A	G	17: 20,914,203	L45P	probably benign	Het
Vmn2r111	C	T	17: 22,571,161	W288*	probably null	Het
Vmn2r118	T	C	17: 55,608,421	S510G	possibly damaging	Het
Wdr60	C	T	12: 116,211,842	W905*	probably null	Het
Zgpat	T	A	2: 181,365,646		probably benign	Het
Zzef1	G	T	11: 72,886,772	G1810C	probably null	Het

Other mutations in Eif3f

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02690:Eif3f	APN	7	108934718	missense	probably damaging	1.00
IGL02821:Eif3f	APN	7	108934674	unclassified	probably benign
IGL02821:Eif3f	APN	7	108934675	unclassified	probably benign
R0010:Eif3f	UTSW	7	108941005	missense	possibly damaging	0.93
R1054:Eif3f	UTSW	7	108937817	critical splice donor site	probably null
R1886:Eif3f	UTSW	7	108940751	missense	probably benign	0.08
R2877:Eif3f	UTSW	7	108934812	splice site	probably null
R3882:Eif3f	UTSW	7	108940955	missense	possibly damaging	0.88
R4471:Eif3f	UTSW	7	108940946	missense	possibly damaging	0.79
R4472:Eif3f	UTSW	7	108940946	missense	possibly damaging	0.79
R4817:Eif3f	UTSW	7	108937775	missense	probably damaging	0.99
R4953:Eif3f	UTSW	7	108934640	unclassified	probably benign
R5744:Eif3f	UTSW	7	108938417	missense	probably damaging	0.99
R7162:Eif3f	UTSW	7	108940731	missense	probably benign	0.02
R7235:Eif3f	UTSW	7	108938088	missense	possibly damaging	0.78
R7445:Eif3f	UTSW	7	108934658	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGCCTTCTCAAGAGGAAGAC -3'
(R):5'- GCTAACTATTGGTTCTGGCCTC -3'

Sequencing Primer
(F):5'- GGAAGACACTAACTTTGACTGC -3'
(R):5'- CTCATATGGATGACTATATGGCCCG -3'

Posted On

2015-03-18