Incidental Mutation 'R5832:Crisp1'
ID449383
Institutional Source Beutler Lab
Gene Symbol Crisp1
Ensembl Gene ENSMUSG00000025431
Gene Namecysteine-rich secretory protein 1
SynonymsAeg1, CRISP-1
MMRRC Submission 044054-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5832 (G1)
Quality Score225
Status Not validated
Chromosome17
Chromosomal Location40293758-40319207 bp(-) (GRCm38)
Type of Mutationsplice site (6 bp from exon)
DNA Base Change (assembly) A to G at 40301317 bp
ZygosityHeterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000026498 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026498]
Predicted Effect probably null
Transcript: ENSMUST00000026498
SMART Domains Protein: ENSMUSP00000026498
Gene: ENSMUSG00000025431

DomainStartEndE-ValueType
signal peptide 1 19 N/A INTRINSIC
SCP 37 178 1.89e-55 SMART
Pfam:Crisp 190 244 4.4e-22 PFAM
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.8%
  • 20x: 96.7%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for a null allele exhibit reduced ability of sperm to fertilize eggs but no reduction in fertility. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930505A04Rik C T 11: 30,426,349 V173M probably damaging Het
Adgrv1 G A 13: 81,103,302 S6232F possibly damaging Het
Alox12 T C 11: 70,253,280 E129G probably damaging Het
Anxa13 C A 15: 58,341,993 noncoding transcript Het
Arfgef3 C T 10: 18,630,420 G878D probably damaging Het
Asnsd1 T C 1: 53,347,475 D331G probably damaging Het
Eml5 T C 12: 98,876,188 N217S probably benign Het
Fat1 A T 8: 45,017,423 Y1463F possibly damaging Het
Fhod3 T C 18: 25,090,695 W1033R probably damaging Het
Galr2 T A 11: 116,281,631 L49Q probably damaging Het
Gstm5 T C 3: 107,897,537 V115A probably benign Het
Gtpbp2 C T 17: 46,167,862 T535I probably damaging Het
Hk1 T C 10: 62,292,365 E326G probably benign Het
Igfn1 A G 1: 135,974,795 V388A probably damaging Het
Iqgap2 C T 13: 95,675,372 R707H probably damaging Het
Kitl C A 10: 100,080,020 P137H probably damaging Het
Lamp3 A T 16: 19,701,320 Y38N probably damaging Het
Lmo7 A T 14: 101,884,213 N5I probably damaging Het
Mc3r T A 2: 172,249,430 C191S probably benign Het
Mep1a G A 17: 43,478,164 H574Y probably benign Het
Mybpc3 A G 2: 91,119,175 probably null Het
Nav2 A T 7: 49,548,069 probably null Het
Patz1 C T 11: 3,306,277 P521L probably benign Het
Pramef17 A G 4: 143,991,962 S304P probably damaging Het
Prkcd T C 14: 30,605,821 T103A probably damaging Het
Pttg1ip T C 10: 77,584,025 probably null Het
Rcbtb2 C A 14: 73,166,822 Q85K possibly damaging Het
Rdh16f1 T A 10: 127,788,749 V152E probably damaging Het
Rsph4a A G 10: 33,909,502 I470V probably benign Het
Sarnp T C 10: 128,848,312 probably null Het
Slc39a6 C A 18: 24,601,612 V7L possibly damaging Het
Slco1a4 A G 6: 141,819,544 I324T probably benign Het
Spata31d1a A T 13: 59,701,566 V916E probably damaging Het
Srgap1 T G 10: 121,840,914 T392P probably damaging Het
Tbc1d20 A T 2: 152,311,362 M271L possibly damaging Het
Tbc1d22b T C 17: 29,570,647 I161T possibly damaging Het
Tcof1 G T 18: 60,819,539 N918K unknown Het
Tnr A G 1: 159,886,122 T707A probably benign Het
Trim66 A G 7: 109,455,202 F1267S probably damaging Het
Trpm6 A G 19: 18,786,819 H263R possibly damaging Het
Tshz2 T A 2: 169,884,045 V187D possibly damaging Het
Ube2f T G 1: 91,285,324 V176G possibly damaging Het
Vmn2r109 C T 17: 20,541,056 A680T probably benign Het
Vmn2r77 T A 7: 86,811,462 C665* probably null Het
Zfp954 A G 7: 7,115,390 V385A probably damaging Het
Other mutations in Crisp1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02794:Crisp1 APN 17 40313066 missense unknown
IGL03034:Crisp1 APN 17 40307728 missense probably benign 0.11
R0670:Crisp1 UTSW 17 40305110 nonsense probably null
R1672:Crisp1 UTSW 17 40308869 missense possibly damaging 0.93
R2264:Crisp1 UTSW 17 40313074 start codon destroyed probably null
R2904:Crisp1 UTSW 17 40313004 critical splice donor site probably null
R4672:Crisp1 UTSW 17 40294513 critical splice acceptor site probably null
R5079:Crisp1 UTSW 17 40308976 splice site probably null
R6051:Crisp1 UTSW 17 40305126 missense possibly damaging 0.59
R7318:Crisp1 UTSW 17 40307777 missense possibly damaging 0.91
R7354:Crisp1 UTSW 17 40319180 start gained probably benign
R7897:Crisp1 UTSW 17 40307765 missense probably benign 0.06
R7980:Crisp1 UTSW 17 40307765 missense probably benign 0.06
Predicted Primers PCR Primer
(F):5'- AGAACTGATGAGTTAGCCATTTCAC -3'
(R):5'- GTCATTTAAGTAAGTCCAGAAGGC -3'

Sequencing Primer
(F):5'- TGAGTTAGCCATTTCACAAATTTTG -3'
(R):5'- GTCCAGAAGGCATATAGTAACAAAC -3'
Posted On2016-12-20