Incidental Mutation 'R7682:Rassf7'
ID 592823
Institutional Source Beutler Lab
Gene Symbol Rassf7
Ensembl Gene ENSMUSG00000038618
Gene Name Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
Synonyms 2400009B11Rik
MMRRC Submission 045748-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.137) question?
Stock # R7682 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 140795773-140798571 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 140797847 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 296 (N296D)
Ref Sequence ENSEMBL: ENSMUSP00000038444 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026573] [ENSMUST00000046890] [ENSMUST00000127613] [ENSMUST00000133763] [ENSMUST00000141804] [ENSMUST00000148975] [ENSMUST00000153081] [ENSMUST00000170841] [ENSMUST00000209500] [ENSMUST00000210993]
AlphaFold Q9DD19
Predicted Effect probably benign
Transcript: ENSMUST00000026573
SMART Domains Protein: ENSMUSP00000026573
Gene: ENSMUSG00000025500

DomainStartEndE-ValueType
coiled coil region 114 170 N/A INTRINSIC
low complexity region 286 298 N/A INTRINSIC
Pfam:LTD 375 482 1.3e-13 PFAM
low complexity region 567 578 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000046890
AA Change: N296D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000038444
Gene: ENSMUSG00000038618
AA Change: N296D

DomainStartEndE-ValueType
RA 6 89 1.67e-19 SMART
low complexity region 181 215 N/A INTRINSIC
low complexity region 217 244 N/A INTRINSIC
coiled coil region 259 300 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000127613
Predicted Effect probably benign
Transcript: ENSMUST00000133763
SMART Domains Protein: ENSMUSP00000118313
Gene: ENSMUSG00000038618

DomainStartEndE-ValueType
RA 6 89 1.67e-19 SMART
low complexity region 181 200 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000141804
SMART Domains Protein: ENSMUSP00000115948
Gene: ENSMUSG00000038618

DomainStartEndE-ValueType
RA 6 89 1.67e-19 SMART
low complexity region 181 215 N/A INTRINSIC
low complexity region 217 243 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000148975
SMART Domains Protein: ENSMUSP00000118078
Gene: ENSMUSG00000038618

DomainStartEndE-ValueType
Blast:RA 6 35 5e-13 BLAST
PDB:2CS4|A 7 35 2e-11 PDB
low complexity region 36 47 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000153081
SMART Domains Protein: ENSMUSP00000123128
Gene: ENSMUSG00000038618

DomainStartEndE-ValueType
RA 6 89 1.67e-19 SMART
low complexity region 181 199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000170841
SMART Domains Protein: ENSMUSP00000130905
Gene: ENSMUSG00000025500

DomainStartEndE-ValueType
coiled coil region 124 180 N/A INTRINSIC
low complexity region 296 308 N/A INTRINSIC
SCOP:d1ifra_ 385 487 1e-22 SMART
low complexity region 577 588 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000209500
AA Change: N296D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably benign
Transcript: ENSMUST00000210993
Meta Mutation Damage Score 0.1054 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (62/62)
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2310034C09Rik T A 16: 88,556,373 (GRCm39) S196T probably benign Het
Abcc5 T C 16: 20,186,803 (GRCm39) D977G probably damaging Het
Acot2 T C 12: 84,034,698 (GRCm39) V8A probably benign Het
Adamts9 T A 6: 92,857,679 (GRCm39) I870F possibly damaging Het
Adgrl3 G A 5: 81,942,407 (GRCm39) V1414I probably damaging Het
Aggf1 T C 13: 95,504,934 (GRCm39) K275R probably benign Het
Alpk1 T C 3: 127,466,195 (GRCm39) I1104V possibly damaging Het
Angptl7 A T 4: 148,582,539 (GRCm39) I119N probably damaging Het
Ank3 A T 10: 69,824,065 (GRCm39) E129D possibly damaging Het
Brca2 T A 5: 150,466,618 (GRCm39) H2127Q probably benign Het
Brd4 A G 17: 32,420,134 (GRCm39) F1008S unknown Het
Car2 T C 3: 14,953,025 (GRCm39) S56P probably damaging Het
Casp3 A G 8: 47,085,420 (GRCm39) Y41C probably benign Het
Ces4a A T 8: 105,873,297 (GRCm39) T381S probably benign Het
Clstn1 A G 4: 149,710,558 (GRCm39) T77A possibly damaging Het
Cul7 G T 17: 46,966,521 (GRCm39) V615L probably benign Het
Dnpep A G 1: 75,293,384 (GRCm39) F24L probably damaging Het
Efcab3 T A 11: 104,855,174 (GRCm39) probably null Het
Emsy G A 7: 98,239,905 (GRCm39) R1263W probably damaging Het
Fam124b A T 1: 80,191,282 (GRCm39) C34S possibly damaging Het
Fan1 C A 7: 64,022,512 (GRCm39) G247V probably benign Het
Fbxl9 C A 8: 106,041,916 (GRCm39) R304L possibly damaging Het
Glipr1l3 T G 10: 111,977,777 (GRCm39) H218P probably benign Het
Gm14305 T A 2: 176,412,703 (GRCm39) S198R probably benign Het
Gm14326 T C 2: 177,590,274 (GRCm39) Y29C probably damaging Het
Gm45713 C T 7: 44,783,426 (GRCm39) V147I probably benign Het
Gpr149 C T 3: 62,438,160 (GRCm39) D666N probably damaging Het
Gpr183 A G 14: 122,192,152 (GRCm39) I123T possibly damaging Het
Il7r A T 15: 9,513,013 (GRCm39) H165Q probably damaging Het
Krt73 A G 15: 101,710,480 (GRCm39) F85L probably benign Het
Lrrc8d A G 5: 105,960,657 (GRCm39) T356A probably damaging Het
Marco A G 1: 120,421,771 (GRCm39) probably null Het
Mettl14 T A 3: 123,177,253 (GRCm39) E49D possibly damaging Het
Mrap T A 16: 90,546,110 (GRCm39) probably null Het
Mrps34 C T 17: 25,114,852 (GRCm39) A152V probably benign Het
Nfasc T A 1: 132,501,511 (GRCm39) Y1163F unknown Het
Nfic T C 10: 81,256,334 (GRCm39) D110G probably damaging Het
Nip7 T C 8: 107,783,751 (GRCm39) V28A possibly damaging Het
Odam T C 5: 88,040,287 (GRCm39) F251S possibly damaging Het
Olfm5 T A 7: 103,810,979 (GRCm39) Q53L probably null Het
P2ry13 T G 3: 59,117,545 (GRCm39) M78L probably benign Het
Pacs1 T C 19: 5,202,727 (GRCm39) E385G probably damaging Het
Pip5k1b C T 19: 24,337,343 (GRCm39) G315D probably damaging Het
Pla2g4a C A 1: 149,762,022 (GRCm39) R145L probably damaging Het
Pramel26 A G 4: 143,537,290 (GRCm39) L347S probably benign Het
Rars1 T C 11: 35,719,579 (GRCm39) Q81R probably benign Het
Rcsd1 C T 1: 165,485,262 (GRCm39) A94T probably benign Het
Rgs8 T C 1: 153,566,668 (GRCm39) F73S probably damaging Het
Rilpl2 A C 5: 124,616,043 (GRCm39) Y36D probably damaging Het
Rpl10l A G 12: 66,331,004 (GRCm39) V43A probably benign Het
Sesn2 T C 4: 132,224,200 (GRCm39) I403V probably damaging Het
Slc10a4 C T 5: 73,164,453 (GRCm39) S15L unknown Het
Slc7a5 T C 8: 122,633,879 (GRCm39) Y156C probably damaging Het
Spata18 A G 5: 73,826,008 (GRCm39) H105R Het
Specc1l A G 10: 75,081,636 (GRCm39) D344G probably damaging Het
Syne1 A G 10: 5,112,461 (GRCm39) V415A probably benign Het
Thbs4 T G 13: 92,912,070 (GRCm39) D220A probably benign Het
Tmco5 T C 2: 116,716,752 (GRCm39) S152P probably benign Het
Tmem35b A T 4: 127,022,734 (GRCm39) D125V probably damaging Het
Trim17 A G 11: 58,857,634 (GRCm39) E155G possibly damaging Het
Vmn2r11 A C 5: 109,195,481 (GRCm39) V615G probably benign Het
Vmn2r88 A T 14: 51,655,906 (GRCm39) Q705L Het
Vmn2r93 A G 17: 18,525,583 (GRCm39) R414G probably benign Het
Xirp2 T C 2: 67,339,193 (GRCm39) L478P probably damaging Het
Other mutations in Rassf7
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02098:Rassf7 APN 7 140,798,203 (GRCm39) missense possibly damaging 0.95
R0883:Rassf7 UTSW 7 140,796,903 (GRCm39) splice site probably benign
R1275:Rassf7 UTSW 7 140,797,060 (GRCm39) missense probably damaging 1.00
R1616:Rassf7 UTSW 7 140,796,645 (GRCm39) missense probably damaging 1.00
R5546:Rassf7 UTSW 7 140,796,973 (GRCm39) splice site probably null
R5597:Rassf7 UTSW 7 140,797,024 (GRCm39) missense probably damaging 1.00
R5663:Rassf7 UTSW 7 140,797,003 (GRCm39) missense probably damaging 1.00
R6250:Rassf7 UTSW 7 140,797,156 (GRCm39) missense probably damaging 0.98
R6806:Rassf7 UTSW 7 140,796,722 (GRCm39) missense probably damaging 1.00
R6817:Rassf7 UTSW 7 140,797,360 (GRCm39) missense probably damaging 1.00
R6962:Rassf7 UTSW 7 140,797,503 (GRCm39) missense possibly damaging 0.86
R7054:Rassf7 UTSW 7 140,797,556 (GRCm39) missense probably benign 0.01
R7562:Rassf7 UTSW 7 140,797,101 (GRCm39) nonsense probably null
R9308:Rassf7 UTSW 7 140,798,063 (GRCm39) missense probably benign
R9508:Rassf7 UTSW 7 140,796,924 (GRCm39) nonsense probably null
X0026:Rassf7 UTSW 7 140,798,045 (GRCm39) nonsense probably null
Z1088:Rassf7 UTSW 7 140,797,058 (GRCm39) missense probably damaging 1.00
Z1177:Rassf7 UTSW 7 140,798,198 (GRCm39) missense probably benign 0.34
Predicted Primers PCR Primer
(F):5'- AACTTCGCTACCTGCTACAC -3'
(R):5'- ACACTAGGATGTGGCTCTGG -3'

Sequencing Primer
(F):5'- GCTACACAGCCAGTCTCAACTCTC -3'
(R):5'- CCTGGAGTTCTCCTCTATTTGG -3'
Posted On 2019-11-12