Mutagenetix > Incidental Mutation

Incidental Mutation 'R0654:Map3k21'

62435

Institutional Source

Beutler Lab

Gene Symbol

Map3k21

Ensembl Gene

ENSMUSG00000031853

Gene Name

mitogen-activated protein kinase kinase kinase 21

Synonyms

BC021891

MMRRC Submission

038839-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.151) question?

Stock #

R0654 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

126637189-126674179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 126668759 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Isoleucine at position 782 (L782I)

Ref Sequence

ENSEMBL: ENSMUSP00000034316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034316]

AlphaFold

Q8VDG6

Predicted Effect

probably benign
Transcript: ENSMUST00000034316
AA Change: L782I

PolyPhen 2 Score 0.066 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000034316
Gene: ENSMUSG00000031853
AA Change: L782I

Domain	Start	End	E-Value	Type
SH3	27	87	1.1e-18	SMART
TyrKc	110	382	6.04e-82	SMART
coiled coil region	402	474	N/A	INTRINSIC
low complexity region	478	492	N/A	INTRINSIC
low complexity region	661	677	N/A	INTRINSIC
low complexity region	740	758	N/A	INTRINSIC
low complexity region	766	788	N/A	INTRINSIC

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Asb13	A	C	13: 3,692,092 (GRCm39)	H24P	probably damaging	Het
Avpr1b	T	A	1: 131,527,480 (GRCm39)	M1K	probably null	Het
Baz1a	T	C	12: 54,958,182 (GRCm39)	E1023G	probably benign	Het
Bpifb5	A	G	2: 154,070,820 (GRCm39)	T204A	probably benign	Het
Cfap157	C	T	2: 32,669,954 (GRCm39)	V210M	probably damaging	Het
Clock	A	G	5: 76,374,976 (GRCm39)	V731A	possibly damaging	Het
Cntn6	C	T	6: 104,753,389 (GRCm39)	T447I	probably benign	Het
Dchs1	A	G	7: 105,421,556 (GRCm39)	I288T	probably damaging	Het
Far2	T	C	6: 148,076,639 (GRCm39)	F494S	possibly damaging	Het
Fibin	T	A	2: 110,192,962 (GRCm39)	D60V	probably damaging	Het
Fnd3c2	G	A	X: 105,290,760 (GRCm39)	T302I	possibly damaging	Het
Foxp3	A	G	X: 7,457,639 (GRCm39)	I281V	probably benign	Het
Fryl	A	G	5: 73,240,715 (GRCm39)	I1295T	probably benign	Het
Gpr82	T	C	X: 13,531,829 (GRCm39)	S126P	probably benign	Het
Gria4	T	A	9: 4,464,372 (GRCm39)	Q530L	probably benign	Het
Hivep1	A	C	13: 42,313,232 (GRCm39)	D1824A	probably benign	Het
Kif13a	A	G	13: 46,966,218 (GRCm39)	V400A	possibly damaging	Het
Nphs2	A	C	1: 156,146,317 (GRCm39)	T98P	probably damaging	Het
Pdss2	CGGAG	CG	10: 43,097,927 (GRCm39)		probably benign	Het
Pkd2l1	A	G	19: 44,146,070 (GRCm39)		probably null	Het
Rbm28	A	T	6: 29,128,577 (GRCm39)	S48R	probably damaging	Het
Rimkla	C	T	4: 119,335,177 (GRCm39)	V69M	probably damaging	Het
Scg3	T	A	9: 75,573,017 (GRCm39)	I305F	probably damaging	Het
Slc22a1	T	A	17: 12,881,679 (GRCm39)	N310I	probably damaging	Het
Slc26a9	T	C	1: 131,692,768 (GRCm39)	V700A	probably benign	Het
Snx15	T	C	19: 6,171,915 (GRCm39)	I140V	probably benign	Het
Spryd3	C	T	15: 102,036,969 (GRCm39)		probably null	Het
Srebf1	T	C	11: 60,094,942 (GRCm39)	T486A	probably benign	Het
Syne2	AGAGTGAG	AGAGTGAGTGAG	12: 76,144,734 (GRCm39)		probably null	Het
Tbl1xr1	T	A	3: 22,258,158 (GRCm39)		probably null	Het
Tmcc1	A	T	6: 116,019,951 (GRCm39)	H281Q	probably benign	Het
Ttc39b	T	A	4: 83,159,938 (GRCm39)	M413L	probably benign	Het
Vmn2r101	C	A	17: 19,810,373 (GRCm39)	H386Q	probably benign	Het
Zbtb47	T	C	9: 121,592,327 (GRCm39)	F251L	probably benign	Het

Other mutations in Map3k21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00494:Map3k21	APN	8	126,671,412 (GRCm39)	missense	possibly damaging	0.52
IGL01919:Map3k21	APN	8	126,668,871 (GRCm39)	missense	probably damaging	0.97
IGL02065:Map3k21	APN	8	126,668,397 (GRCm39)	missense	probably benign	0.01
IGL02123:Map3k21	APN	8	126,652,849 (GRCm39)	missense	probably damaging	1.00
IGL02127:Map3k21	APN	8	126,668,886 (GRCm39)	missense	probably benign
IGL02863:Map3k21	APN	8	126,654,280 (GRCm39)	missense	probably benign	0.02
IGL03194:Map3k21	APN	8	126,650,801 (GRCm39)	missense	possibly damaging	0.90
PIT4142001:Map3k21	UTSW	8	126,664,047 (GRCm39)	missense	probably damaging	0.98
R0238:Map3k21	UTSW	8	126,671,709 (GRCm39)	missense	possibly damaging	0.67
R0238:Map3k21	UTSW	8	126,671,709 (GRCm39)	missense	possibly damaging	0.67
R0454:Map3k21	UTSW	8	126,668,858 (GRCm39)	missense	probably benign
R1141:Map3k21	UTSW	8	126,668,471 (GRCm39)	missense	probably benign	0.32
R1177:Map3k21	UTSW	8	126,671,577 (GRCm39)	missense	probably benign	0.31
R1463:Map3k21	UTSW	8	126,668,876 (GRCm39)	missense	probably benign	0.00
R1472:Map3k21	UTSW	8	126,668,417 (GRCm39)	missense	probably benign
R1759:Map3k21	UTSW	8	126,671,519 (GRCm39)	missense	probably benign
R1988:Map3k21	UTSW	8	126,654,294 (GRCm39)	missense	probably benign	0.07
R2058:Map3k21	UTSW	8	126,665,461 (GRCm39)	missense	probably benign	0.01
R2117:Map3k21	UTSW	8	126,650,781 (GRCm39)	missense	probably benign	0.19
R2157:Map3k21	UTSW	8	126,664,005 (GRCm39)	missense	probably benign
R2436:Map3k21	UTSW	8	126,668,354 (GRCm39)	nonsense	probably null
R2507:Map3k21	UTSW	8	126,666,677 (GRCm39)	missense	possibly damaging	0.73
R3125:Map3k21	UTSW	8	126,668,593 (GRCm39)	missense	probably benign	0.26
R3746:Map3k21	UTSW	8	126,661,839 (GRCm39)	missense	probably damaging	1.00
R4016:Map3k21	UTSW	8	126,637,924 (GRCm39)	missense	probably damaging	1.00
R4647:Map3k21	UTSW	8	126,668,850 (GRCm39)	missense	probably benign
R4648:Map3k21	UTSW	8	126,668,850 (GRCm39)	missense	probably benign
R4864:Map3k21	UTSW	8	126,654,294 (GRCm39)	missense	probably benign	0.04
R5642:Map3k21	UTSW	8	126,665,563 (GRCm39)	missense	probably benign	0.17
R5694:Map3k21	UTSW	8	126,671,507 (GRCm39)	missense	probably benign	0.04
R5950:Map3k21	UTSW	8	126,668,499 (GRCm39)	missense	possibly damaging	0.93
R5982:Map3k21	UTSW	8	126,638,169 (GRCm39)	missense	probably damaging	1.00
R6440:Map3k21	UTSW	8	126,637,876 (GRCm39)	missense	probably damaging	1.00
R6550:Map3k21	UTSW	8	126,664,031 (GRCm39)	missense	probably damaging	1.00
R6664:Map3k21	UTSW	8	126,668,610 (GRCm39)	missense	probably benign	0.01
R6668:Map3k21	UTSW	8	126,652,852 (GRCm39)	missense	possibly damaging	0.60
R6788:Map3k21	UTSW	8	126,666,605 (GRCm39)	missense	probably benign	0.28
R7369:Map3k21	UTSW	8	126,637,855 (GRCm39)	missense	possibly damaging	0.86
R7371:Map3k21	UTSW	8	126,661,804 (GRCm39)	missense	probably damaging	0.99
R7381:Map3k21	UTSW	8	126,671,717 (GRCm39)	missense	possibly damaging	0.83
R7388:Map3k21	UTSW	8	126,654,336 (GRCm39)	missense	probably damaging	1.00
R7397:Map3k21	UTSW	8	126,661,855 (GRCm39)	missense	probably damaging	1.00
R7497:Map3k21	UTSW	8	126,654,340 (GRCm39)	missense	probably damaging	0.99
R7562:Map3k21	UTSW	8	126,665,539 (GRCm39)	missense	probably damaging	1.00
R7564:Map3k21	UTSW	8	126,654,447 (GRCm39)	critical splice donor site	probably null
R7824:Map3k21	UTSW	8	126,637,702 (GRCm39)	missense	probably benign	0.01
R8286:Map3k21	UTSW	8	126,637,498 (GRCm39)	missense	probably benign	0.00
R8351:Map3k21	UTSW	8	126,671,472 (GRCm39)	missense	probably benign	0.00
R8451:Map3k21	UTSW	8	126,671,472 (GRCm39)	missense	probably benign	0.00
R8461:Map3k21	UTSW	8	126,671,361 (GRCm39)	missense	probably benign	0.05
R9005:Map3k21	UTSW	8	126,637,471 (GRCm39)	missense
R9074:Map3k21	UTSW	8	126,664,050 (GRCm39)	missense	probably damaging	0.98
R9156:Map3k21	UTSW	8	126,665,463 (GRCm39)	missense	possibly damaging	0.81
R9217:Map3k21	UTSW	8	126,638,027 (GRCm39)	missense	possibly damaging	0.47
R9474:Map3k21	UTSW	8	126,650,903 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGTCAGGAAACTTCACGGAGCCC -3'
(R):5'- CCTTTGTCTCCTACAATGGCACAGC -3'

Sequencing Primer
(F):5'- CAATCAAGTCCCAGTTCCCTTCT -3'
(R):5'- ACAGCCTGGCAGTCTCTC -3'

Posted On

2013-07-30