Incidental Mutation 'R8730:Adgrg3'
| ID |
662708 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Adgrg3
|
| Ensembl Gene |
ENSMUSG00000060470 |
| Gene Name |
adhesion G protein-coupled receptor G3 |
| Synonyms |
Pb99, A030001G24Rik, Gpr97 |
| MMRRC Submission |
068578-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R8730 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
8 |
| Chromosomal Location |
95744320-95771878 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to A
at 95766556 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Histidine
at position 409
(R409H)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000051079
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000051259]
|
| AlphaFold |
Q8R0T6 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000051259
AA Change: R409H
PolyPhen 2
Score 0.095 (Sensitivity: 0.93; Specificity: 0.85)
|
| SMART Domains |
Protein: ENSMUSP00000051079
Gene: ENSMUSG00000060470
AA Change: R409H
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
18 |
N/A |
INTRINSIC |
|
GPS
|
209 |
256 |
3.45e-11 |
SMART |
|
Pfam:7tm_2
|
260 |
509 |
5.1e-33 |
PFAM |
|
low complexity region
|
520 |
531 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000212570
|
| Meta Mutation Damage Score |
0.4192 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.3%
- 20x: 97.3%
|
| Validation Efficiency |
100% (57/57) |
| MGI Phenotype |
PHENOTYPE: Homozygous null mice display normal B and T cell development. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 55 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam22 |
A |
C |
5: 8,208,830 (GRCm39) |
S200A |
probably benign |
Het |
| Aldh1l2 |
C |
T |
10: 83,342,506 (GRCm39) |
V548M |
possibly damaging |
Het |
| Ampd1 |
T |
A |
3: 102,992,676 (GRCm39) |
C143* |
probably null |
Het |
| Angpt4 |
A |
G |
2: 151,771,467 (GRCm39) |
Q261R |
probably damaging |
Het |
| Ccar1 |
T |
C |
10: 62,601,191 (GRCm39) |
K491E |
probably damaging |
Het |
| Cd177 |
A |
T |
7: 24,457,501 (GRCm39) |
M180K |
possibly damaging |
Het |
| Dcst2 |
C |
T |
3: 89,280,553 (GRCm39) |
R620C |
probably damaging |
Het |
| Dcun1d4 |
T |
A |
5: 73,688,832 (GRCm39) |
|
probably benign |
Het |
| Dhrs1 |
T |
A |
14: 55,980,978 (GRCm39) |
T103S |
probably benign |
Het |
| Dnah2 |
A |
G |
11: 69,384,087 (GRCm39) |
L1043P |
possibly damaging |
Het |
| Eci3 |
A |
T |
13: 35,144,405 (GRCm39) |
N19K |
probably benign |
Het |
| Ets1 |
A |
T |
9: 32,649,614 (GRCm39) |
D317V |
probably damaging |
Het |
| Fem1c |
T |
C |
18: 46,638,668 (GRCm39) |
I445V |
possibly damaging |
Het |
| Gabpb1 |
T |
C |
2: 126,492,484 (GRCm39) |
I176M |
possibly damaging |
Het |
| Gm6563 |
A |
T |
19: 23,653,429 (GRCm39) |
K73I |
probably damaging |
Het |
| Grap2 |
A |
G |
15: 80,532,140 (GRCm39) |
R252G |
possibly damaging |
Het |
| Gsx1 |
T |
C |
5: 147,126,651 (GRCm39) |
L158P |
probably damaging |
Het |
| Hand2 |
T |
A |
8: 57,775,468 (GRCm39) |
V176E |
probably benign |
Het |
| Hey2 |
G |
T |
10: 30,718,622 (GRCm39) |
T8K |
possibly damaging |
Het |
| Igsf3 |
T |
C |
3: 101,334,532 (GRCm39) |
I203T |
probably benign |
Het |
| Itgax |
G |
A |
7: 127,739,066 (GRCm39) |
|
probably null |
Het |
| Kcnn2 |
A |
G |
18: 45,725,139 (GRCm39) |
I212V |
possibly damaging |
Het |
| Kcnu1 |
G |
T |
8: 26,403,708 (GRCm39) |
V740L |
probably damaging |
Het |
| Kcnv1 |
A |
G |
15: 44,972,797 (GRCm39) |
I362T |
probably damaging |
Het |
| Klhdc9 |
C |
T |
1: 171,186,488 (GRCm39) |
G316D |
probably damaging |
Het |
| Mcmbp |
T |
C |
7: 128,317,738 (GRCm39) |
E169G |
probably damaging |
Het |
| Muc20 |
G |
T |
16: 32,599,490 (GRCm39) |
H645N |
probably benign |
Het |
| Nrm |
A |
G |
17: 36,175,423 (GRCm39) |
T52A |
probably benign |
Het |
| Or13a23-ps1 |
T |
C |
7: 140,119,197 (GRCm39) |
S256P |
unknown |
Het |
| Or14c41 |
A |
G |
7: 86,235,259 (GRCm39) |
K259E |
probably benign |
Het |
| Or2y3 |
A |
T |
17: 38,392,925 (GRCm39) |
*315K |
probably null |
Het |
| Or4c105 |
T |
A |
2: 88,648,043 (GRCm39) |
M176K |
possibly damaging |
Het |
| Or4g7 |
T |
A |
2: 111,309,934 (GRCm39) |
D268E |
probably damaging |
Het |
| Or51f23 |
T |
C |
7: 102,453,348 (GRCm39) |
V221A |
probably benign |
Het |
| Pde11a |
A |
T |
2: 75,889,334 (GRCm39) |
N713K |
probably damaging |
Het |
| Pfpl |
T |
C |
19: 12,405,944 (GRCm39) |
L65S |
probably damaging |
Het |
| Prox1 |
A |
G |
1: 189,894,238 (GRCm39) |
V69A |
possibly damaging |
Het |
| Prss39 |
A |
G |
1: 34,539,198 (GRCm39) |
H146R |
probably damaging |
Het |
| Pxt1 |
A |
T |
17: 29,153,702 (GRCm39) |
F44I |
possibly damaging |
Het |
| Rbp3 |
A |
G |
14: 33,677,795 (GRCm39) |
D581G |
probably benign |
Het |
| Rims2 |
A |
G |
15: 39,381,239 (GRCm39) |
T1057A |
probably benign |
Het |
| Robo1 |
G |
A |
16: 72,786,495 (GRCm39) |
G836R |
probably benign |
Het |
| Slc34a3 |
A |
T |
2: 25,122,057 (GRCm39) |
S155T |
possibly damaging |
Het |
| Slc35d1 |
T |
C |
4: 103,030,951 (GRCm39) |
Y308C |
|
Het |
| Slfn9 |
T |
C |
11: 82,878,194 (GRCm39) |
I312V |
possibly damaging |
Het |
| St3gal5 |
T |
A |
6: 72,130,461 (GRCm39) |
L351Q |
probably damaging |
Het |
| Tanc1 |
A |
G |
2: 59,601,590 (GRCm39) |
D157G |
probably benign |
Het |
| Tmprss11g |
C |
T |
5: 86,638,837 (GRCm39) |
|
probably null |
Het |
| Tnpo1 |
A |
T |
13: 98,989,916 (GRCm39) |
I745N |
probably benign |
Het |
| Uggt1 |
C |
A |
1: 36,236,624 (GRCm39) |
|
probably null |
Het |
| Ugt8a |
G |
A |
3: 125,732,105 (GRCm39) |
|
probably benign |
Het |
| Urod |
T |
C |
4: 116,850,729 (GRCm39) |
|
probably benign |
Het |
| Vmn1r211 |
A |
T |
13: 23,035,838 (GRCm39) |
Y276* |
probably null |
Het |
| Vwa3a |
T |
A |
7: 120,381,910 (GRCm39) |
S582T |
probably damaging |
Het |
| Zfp869 |
A |
T |
8: 70,159,177 (GRCm39) |
C465* |
probably null |
Het |
|
| Other mutations in Adgrg3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01096:Adgrg3
|
APN |
8 |
95,766,221 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
IGL01724:Adgrg3
|
APN |
8 |
95,766,053 (GRCm39) |
missense |
probably benign |
0.04 |
|
IGL02111:Adgrg3
|
APN |
8 |
95,761,627 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL02142:Adgrg3
|
APN |
8 |
95,766,483 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02577:Adgrg3
|
APN |
8 |
95,766,555 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02940:Adgrg3
|
APN |
8 |
95,760,084 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
IGL03395:Adgrg3
|
APN |
8 |
95,761,701 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0111:Adgrg3
|
UTSW |
8 |
95,761,738 (GRCm39) |
splice site |
probably benign |
|
|
R0288:Adgrg3
|
UTSW |
8 |
95,766,568 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R0403:Adgrg3
|
UTSW |
8 |
95,763,550 (GRCm39) |
missense |
probably benign |
0.07 |
|
R1553:Adgrg3
|
UTSW |
8 |
95,766,896 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R1667:Adgrg3
|
UTSW |
8 |
95,760,001 (GRCm39) |
nonsense |
probably null |
|
|
R1686:Adgrg3
|
UTSW |
8 |
95,759,997 (GRCm39) |
missense |
probably benign |
0.02 |
|
R1872:Adgrg3
|
UTSW |
8 |
95,760,070 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1882:Adgrg3
|
UTSW |
8 |
95,766,943 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1924:Adgrg3
|
UTSW |
8 |
95,762,562 (GRCm39) |
missense |
probably benign |
|
|
R1998:Adgrg3
|
UTSW |
8 |
95,763,296 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2090:Adgrg3
|
UTSW |
8 |
95,766,558 (GRCm39) |
missense |
possibly damaging |
0.54 |
|
R2696:Adgrg3
|
UTSW |
8 |
95,747,702 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3846:Adgrg3
|
UTSW |
8 |
95,767,049 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4013:Adgrg3
|
UTSW |
8 |
95,761,727 (GRCm39) |
splice site |
probably benign |
|
|
R4405:Adgrg3
|
UTSW |
8 |
95,763,536 (GRCm39) |
missense |
probably benign |
0.15 |
|
R4622:Adgrg3
|
UTSW |
8 |
95,767,153 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4878:Adgrg3
|
UTSW |
8 |
95,761,714 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R5101:Adgrg3
|
UTSW |
8 |
95,763,563 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5309:Adgrg3
|
UTSW |
8 |
95,766,492 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5312:Adgrg3
|
UTSW |
8 |
95,766,492 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5353:Adgrg3
|
UTSW |
8 |
95,762,556 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5820:Adgrg3
|
UTSW |
8 |
95,766,221 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R6240:Adgrg3
|
UTSW |
8 |
95,766,544 (GRCm39) |
missense |
probably benign |
0.23 |
|
R6272:Adgrg3
|
UTSW |
8 |
95,762,889 (GRCm39) |
missense |
noncoding transcript |
|
|
R7110:Adgrg3
|
UTSW |
8 |
95,761,591 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R7645:Adgrg3
|
UTSW |
8 |
95,761,392 (GRCm39) |
intron |
probably benign |
|
|
R8178:Adgrg3
|
UTSW |
8 |
95,761,675 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8397:Adgrg3
|
UTSW |
8 |
95,767,141 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8951:Adgrg3
|
UTSW |
8 |
95,761,362 (GRCm39) |
intron |
probably benign |
|
|
R9100:Adgrg3
|
UTSW |
8 |
95,762,891 (GRCm39) |
intron |
probably benign |
|
|
R9523:Adgrg3
|
UTSW |
8 |
95,766,186 (GRCm39) |
missense |
probably benign |
0.06 |
|
R9583:Adgrg3
|
UTSW |
8 |
95,760,071 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9589:Adgrg3
|
UTSW |
8 |
95,760,093 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
X0017:Adgrg3
|
UTSW |
8 |
95,744,398 (GRCm39) |
start codon destroyed |
probably null |
0.53 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCTCAAGCAATGGATATCTGGAG -3'
(R):5'- TAGTGACCAAGACGATGCCC -3'
Sequencing Primer
(F):5'- CTGGAGGAACAGGAGGCAATCTC -3'
(R):5'- CTGTTGGCTCTCCTGGGC -3'
|
| Posted On |
2021-03-08 |
Incidental Mutation