Mutagenetix > Incidental Mutation

Incidental Mutation 'R9097:Stk32a'

691439

Institutional Source

Beutler Lab

Gene Symbol

Stk32a

Ensembl Gene

ENSMUSG00000039954

Gene Name

serine/threonine kinase 32A

Synonyms

A930015B13Rik, YANK1

MMRRC Submission

068912-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R9097 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

43340762-43450546 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 43446497 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 316 (M316L)

Ref Sequence

ENSEMBL: ENSMUSP00000038471 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045477]

AlphaFold

Q8BGW6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000045477
AA Change: M316L

PolyPhen 2 Score 0.618 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000038471
Gene: ENSMUSG00000039954
AA Change: M316L

Domain	Start	End	E-Value	Type
S_TKc	23	281	9.58e-85	SMART
low complexity region	318	339	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.1%

Validation Efficiency

96% (50/52)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Antxrl	G	A	14: 33,793,660 (GRCm39)	V463I	probably benign	Het
Aox1	T	A	1: 58,326,887 (GRCm39)	L162Q	possibly damaging	Het
Arhgap40	A	T	2: 158,389,584 (GRCm39)	M586L	probably benign	Het
Arnt	G	T	3: 95,397,588 (GRCm39)	S535I	probably benign	Het
Bsx	G	T	9: 40,785,636 (GRCm39)	G55C	probably damaging	Het
Ccdc168	T	C	1: 44,098,049 (GRCm39)	I1016M	possibly damaging	Het
Col9a1	A	T	1: 24,224,207 (GRCm39)	I130F	unknown	Het
Cyp51	G	T	5: 4,149,172 (GRCm39)	T235N	possibly damaging	Het
Depdc1a	T	A	3: 159,204,117 (GRCm39)	D55E	probably benign	Het
Dlc1	A	T	8: 37,080,721 (GRCm39)	I10N	probably benign	Het
Dzank1	G	T	2: 144,316,882 (GRCm39)	L767I	possibly damaging	Het
Eif3k	A	T	7: 28,671,660 (GRCm39)	*193K	probably null	Het
Eprs1	C	A	1: 185,139,303 (GRCm39)	A896E	probably benign	Het
Evc2	T	A	5: 37,550,505 (GRCm39)	F840I	possibly damaging	Het
Fut2	G	T	7: 45,300,375 (GRCm39)	H132Q	probably benign	Het
Gbp11	T	C	5: 105,474,347 (GRCm39)	*443W	probably null	Het
Gimd1	T	A	3: 132,340,661 (GRCm39)	M59K	probably benign	Het
Glis2	A	G	16: 4,429,640 (GRCm39)	T228A	probably damaging	Het
Gm21663	G	C	5: 26,147,167 (GRCm39)	C46W	probably benign	Het
Gpr162	A	G	6: 124,836,570 (GRCm39)	I367T	probably benign	Het
Grik1	G	A	16: 87,732,796 (GRCm39)	T692I		Het
Igf2r	G	T	17: 12,910,100 (GRCm39)	A2043D	probably damaging	Het
Il12b	C	A	11: 44,301,107 (GRCm39)	L208M	probably benign	Het
Il12b	T	A	11: 44,301,108 (GRCm39)	L208Q	probably damaging	Het
Lrrk2	C	T	15: 91,557,459 (GRCm39)		probably benign	Het
Map3k9	T	C	12: 81,819,855 (GRCm39)	D133G	possibly damaging	Het
Mrc2	A	G	11: 105,231,398 (GRCm39)	D777G	probably damaging	Het
Mtrr	A	G	13: 68,723,441 (GRCm39)	L157P	probably benign	Het
Nol4l	C	A	2: 153,312,630 (GRCm39)	R226L	probably damaging	Het
Nsmaf	CAAACTTTTAAACTT	CAAACTT	4: 6,416,543 (GRCm39)		probably null	Het
Or4f7	T	A	2: 111,644,196 (GRCm39)	K292*	probably null	Het
Or51f23c-ps1	A	G	7: 102,431,475 (GRCm39)	H264R	probably damaging	Het
Or5p73	A	T	7: 108,064,815 (GRCm39)	I95F	probably damaging	Het
Or6c7	T	A	10: 129,323,647 (GRCm39)	M256K	possibly damaging	Het
Or7g22	G	A	9: 19,048,670 (GRCm39)	C127Y	probably damaging	Het
Or9g8	T	A	2: 85,607,669 (GRCm39)	V247E	probably damaging	Het
Pkn2	C	T	3: 142,515,249 (GRCm39)	R695Q	probably benign	Het
Ppp1r9a	A	G	6: 4,906,012 (GRCm39)	D189G	probably damaging	Het
Prkca	A	G	11: 107,905,061 (GRCm39)	S226P	probably benign	Het
Ptpru	T	C	4: 131,499,843 (GRCm39)	N1267S	probably damaging	Het
Retnlb	T	A	16: 48,638,980 (GRCm39)		probably benign	Het
Rrp12	A	T	19: 41,878,577 (GRCm39)	D189E	probably benign	Het
Serpinb7	G	A	1: 107,377,907 (GRCm39)	C200Y	probably damaging	Het
Sez6	G	A	11: 77,865,121 (GRCm39)	E623K	possibly damaging	Het
Supt6	G	T	11: 78,113,100 (GRCm39)	H948N	probably benign	Het
Sycp2l	A	T	13: 41,300,070 (GRCm39)	D428V	possibly damaging	Het
Tasp1	A	T	2: 139,725,690 (GRCm39)		probably null	Het
Telo2	T	C	17: 25,324,066 (GRCm39)	T550A	probably benign	Het
Tmem131l	A	T	3: 83,850,122 (GRCm39)	N225K	probably damaging	Het
Tnc	T	C	4: 63,888,622 (GRCm39)	T1724A	possibly damaging	Het
Tyk2	C	A	9: 21,020,072 (GRCm39)	E1029*	probably null	Het
Uhmk1	C	T	1: 170,042,879 (GRCm39)		probably benign	Het
Vav2	G	A	2: 27,181,850 (GRCm39)	R330W	probably damaging	Het
Vmn1r16	A	T	6: 57,300,250 (GRCm39)	I124N	probably benign	Het
Vmn1r181	A	G	7: 23,684,444 (GRCm39)	N303S	probably benign	Het
Vmn1r50	G	A	6: 90,085,022 (GRCm39)	V256I	probably benign	Het

Other mutations in Stk32a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00502:Stk32a	APN	18	43,443,510 (GRCm39)	missense	possibly damaging	0.46
IGL00704:Stk32a	APN	18	43,394,314 (GRCm39)	missense	probably damaging	1.00
IGL00813:Stk32a	APN	18	43,443,585 (GRCm39)	missense	probably benign	0.10
IGL02121:Stk32a	APN	18	43,446,572 (GRCm39)	missense	probably benign
IGL02407:Stk32a	APN	18	43,430,576 (GRCm39)	missense	probably benign	0.00
IGL02957:Stk32a	APN	18	43,445,057 (GRCm39)	missense	probably benign
R0004:Stk32a	UTSW	18	43,438,121 (GRCm39)	missense	probably damaging	1.00
R0047:Stk32a	UTSW	18	43,446,443 (GRCm39)	splice site	probably benign
R0047:Stk32a	UTSW	18	43,446,443 (GRCm39)	splice site	probably benign
R0288:Stk32a	UTSW	18	43,438,060 (GRCm39)	splice site	probably null
R0330:Stk32a	UTSW	18	43,446,566 (GRCm39)	missense	probably benign	0.15
R1337:Stk32a	UTSW	18	43,394,414 (GRCm39)	missense	probably benign	0.00
R1559:Stk32a	UTSW	18	43,376,149 (GRCm39)	missense	probably benign	0.32
R1695:Stk32a	UTSW	18	43,446,485 (GRCm39)	nonsense	probably null
R1874:Stk32a	UTSW	18	43,394,381 (GRCm39)	missense	probably damaging	1.00
R1954:Stk32a	UTSW	18	43,345,090 (GRCm39)	missense	probably benign	0.45
R4529:Stk32a	UTSW	18	43,376,044 (GRCm39)	missense	possibly damaging	0.83
R4980:Stk32a	UTSW	18	43,447,113 (GRCm39)	missense	probably benign	0.01
R5124:Stk32a	UTSW	18	43,438,082 (GRCm39)	missense	probably benign	0.00
R5751:Stk32a	UTSW	18	43,438,085 (GRCm39)	missense	possibly damaging	0.74
R5822:Stk32a	UTSW	18	43,446,552 (GRCm39)	missense	probably benign	0.00
R5863:Stk32a	UTSW	18	43,448,209 (GRCm39)	missense	probably benign	0.00
R6167:Stk32a	UTSW	18	43,446,474 (GRCm39)	missense	probably damaging	1.00
R6355:Stk32a	UTSW	18	43,430,659 (GRCm39)	splice site	probably null
R6731:Stk32a	UTSW	18	43,438,143 (GRCm39)	missense	probably damaging	1.00
R7162:Stk32a	UTSW	18	43,430,649 (GRCm39)	nonsense	probably null
R8001:Stk32a	UTSW	18	43,448,209 (GRCm39)	missense	possibly damaging	0.62
R8022:Stk32a	UTSW	18	43,448,166 (GRCm39)	nonsense	probably null
R8485:Stk32a	UTSW	18	43,376,075 (GRCm39)	missense	possibly damaging	0.83
R8994:Stk32a	UTSW	18	43,443,542 (GRCm39)	missense	probably benign	0.03
R9183:Stk32a	UTSW	18	43,394,405 (GRCm39)	missense	probably damaging	1.00
R9258:Stk32a	UTSW	18	43,444,999 (GRCm39)	missense	probably benign	0.27
R9610:Stk32a	UTSW	18	43,430,620 (GRCm39)	missense	probably benign
R9611:Stk32a	UTSW	18	43,430,620 (GRCm39)	missense	probably benign
R9780:Stk32a	UTSW	18	43,375,049 (GRCm39)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- GGAGGGAGCCCAAGTTATTATTC -3'
(R):5'- TAACATAGTAGAGGTTACCCCACC -3'

Sequencing Primer
(F):5'- CAAGTTATTATTCTTGGGCTACCC -3'
(R):5'- TGTCTCTTGCAGAAGACCAG -3'

Posted On

2021-12-30