Mutagenetix > Incidental Mutation

Incidental Mutation 'R9611:Prl5a1'

724238

Institutional Source

Beutler Lab

Gene Symbol

Prl5a1

Ensembl Gene

ENSMUSG00000017064

Gene Name

prolactin family 5, subfamily a, member 1

Synonyms

D13Wsu14e, 1600013P04Rik, Prlpl, PLP-L

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R9611 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

28142484-28151611 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 28145509 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 57 (E57G)

Ref Sequence

ENSEMBL: ENSMUSP00000017208 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000017208]

AlphaFold

Q9JII2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000017208
AA Change: E57G

PolyPhen 2 Score 0.710 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000017208
Gene: ENSMUSG00000017064
AA Change: E57G

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	230	4.8e-50	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110051M20Rik	T	C	2: 91,304,782	D111G	probably damaging	Het
Adamts19	A	T	18: 58,890,327	T265S	probably benign	Het
Ahnak	T	A	19: 9,011,798	L3482Q	probably damaging	Het
Apopt1	T	G	12: 111,733,674	Y163D	probably damaging	Het
Arhgef5	A	G	6: 43,280,956	I1311M	probably damaging	Het
Bcr	T	A	10: 75,154,911	Y750N	probably damaging	Het
Bcr	T	A	10: 75,154,913	Y750*	probably null	Het
Btbd16	T	A	7: 130,805,865	M295K	probably benign	Het
Ccdc88a	T	C	11: 29,477,316	L1007P	possibly damaging	Het
Cnep1r1	A	G	8: 88,133,829	*126W	probably null	Het
Cntnap5b	C	A	1: 99,967,210	P69Q	probably damaging	Het
Col4a3	A	G	1: 82,700,297	M1207V	unknown	Het
Colgalt2	T	A	1: 152,484,994	W261R	probably damaging	Het
Cstf1	T	A	2: 172,373,064	I35N	probably benign	Het
Cul9	A	G	17: 46,519,897	L1690P	possibly damaging	Het
Dscaml1	C	A	9: 45,668,224	N356K	possibly damaging	Het
Ecm2	A	G	13: 49,515,042	N74D	probably benign	Het
Ecm2	A	G	13: 49,527,740	I450M	probably damaging	Het
Emc1	T	A	4: 139,363,724	D460E	probably benign	Het
Fam160b2	T	C	14: 70,586,818	Y493C	probably benign	Het
Fmo5	C	T	3: 97,641,773	R246C	possibly damaging	Het
Fyco1	T	C	9: 123,828,520	T864A	possibly damaging	Het
Galk2	T	C	2: 125,975,298	Y336H	probably damaging	Het
Gcsh	T	A	8: 116,993,386	Y14F	probably benign	Het
Grip1	G	A	10: 120,038,664	E778K	possibly damaging	Het
Gucy2g	T	C	19: 55,206,173	I937M	probably damaging	Het
Hsp90ab1	T	C	17: 45,569,674	I370M	possibly damaging	Het
Hypk	T	C	2: 121,457,673	S72P	probably damaging	Het
Ighv1-26	T	C	12: 114,788,586	Y46C	probably damaging	Het
Kctd13	G	T	7: 126,945,008	G293C	probably damaging	Het
Kmt2d	CTGCTGTTGCTG	CTGCTG	15: 98,845,173		probably benign	Het
Kmt2d	CTGTTG	CTG	15: 98,845,176		probably benign	Het
Krt13	T	C	11: 100,121,492	S2G	probably benign	Het
Mark4	C	A	7: 19,433,413	R467L	possibly damaging	Het
Mrps33	T	A	6: 39,802,488	K91M	probably damaging	Het
Olfr209	A	C	16: 59,361,879	I113S	probably benign	Het
Olfr566	T	G	7: 102,856,940	H114P	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452		probably benign	Het
Peg10	C	CTCA	6: 4,756,453		probably benign	Het
Phldb3	G	A	7: 24,628,947	V639M	probably damaging	Het
Pigyl	A	T	9: 22,158,203	H70L	probably damaging	Het
Pip4k2c	A	C	10: 127,200,200	L266R	probably damaging	Het
Plg	T	C	17: 12,390,326	Y173H	probably benign	Het
Pmp22	G	T	11: 63,133,239	V25F	probably benign	Het
Pot1b	T	A	17: 55,699,995	T41S	probably benign	Het
Ppp1r16b	T	A	2: 158,696,078	Y40N	probably damaging	Het
Prpf3	G	A	3: 95,851,619	R74*	probably null	Het
Psmb10	A	G	8: 105,937,512	F75S	probably benign	Het
Ptprm	C	A	17: 66,693,488	R1167M	probably damaging	Het
Rmnd5b	T	C	11: 51,627,042	I162V	probably damaging	Het
Sik1	T	A	17: 31,854,272	K70M	probably damaging	Het
Slc44a4	C	T	17: 34,928,817	S611F	probably benign	Het
St18	T	C	1: 6,802,923	V294A	probably benign	Het
Stk32a	A	G	18: 43,297,555	I177V	probably benign	Het
Syne2	T	C	12: 76,033,686	F58S	probably benign	Het
Tcf7l2	A	G	19: 55,910,606	D215G	probably null	Het
Tcirg1	A	T	19: 3,903,400	F149Y	probably benign	Het
Tex14	T	A	11: 87,486,258	F143I	probably damaging	Het
Tjp3	A	G	10: 81,283,577	V26A	possibly damaging	Het
Tnfaip8	ACACACTCTC	AC	18: 50,046,841		probably benign	Het
Tpx2	T	A	2: 152,873,204	V115D	probably benign	Het
Tubb2b	C	A	13: 34,127,759	K350N	probably damaging	Het
Vps13b	G	T	15: 35,642,409	G1389V	possibly damaging	Het
Vps39	T	C	2: 120,342,004	R183G	probably damaging	Het
Wdr47	A	T	3: 108,611,413	E72D	probably damaging	Het
Wnk4	G	T	11: 101,268,424	E556*	probably null	Het
Xpo7	T	A	14: 70,688,177	E474V	probably benign	Het
Zfat	A	G	15: 68,179,806	V713A	possibly damaging	Het
Zfp189	G	T	4: 49,530,058	C387F	probably damaging	Het
Zmiz1	T	C	14: 25,650,598	V502A	probably benign	Het

Other mutations in Prl5a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01638:Prl5a1	APN	13	28145439	missense	possibly damaging	0.77
IGL01820:Prl5a1	APN	13	28148700	missense	probably benign	0.34
IGL02682:Prl5a1	APN	13	28145420	missense	probably benign	0.32
R0266:Prl5a1	UTSW	13	28149987	missense	possibly damaging	0.77
R1022:Prl5a1	UTSW	13	28149897	missense	probably damaging	0.97
R1024:Prl5a1	UTSW	13	28149897	missense	probably damaging	0.97
R2098:Prl5a1	UTSW	13	28145505	missense	probably damaging	1.00
R5467:Prl5a1	UTSW	13	28150011	missense	possibly damaging	0.92
R6002:Prl5a1	UTSW	13	28145482	missense	probably benign	0.00
R6026:Prl5a1	UTSW	13	28151264	missense	probably benign	0.43
R6242:Prl5a1	UTSW	13	28142555	nonsense	probably null
R6616:Prl5a1	UTSW	13	28149856	missense	probably benign	0.00
R6733:Prl5a1	UTSW	13	28149936	missense	possibly damaging	0.81
R6979:Prl5a1	UTSW	13	28151206	missense	probably benign	0.32
R7692:Prl5a1	UTSW	13	28150014	missense	probably damaging	1.00
R9610:Prl5a1	UTSW	13	28145509	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- TAACAGAGGACACGGTGGCT -3'
(R):5'- GCTTTCTTGAGCCTATGAATGTGTA -3'

Sequencing Primer
(F):5'- GCTAGACAGGGTTTCACTATACAGC -3'
(R):5'- AGCCTATGAATGTGTATTGTTTGG -3'

Posted On

2022-09-12