Mutagenetix > Incidental Mutation

Incidental Mutation 'R1903:Trrap'

209930

Institutional Source

Beutler Lab

Gene Symbol

Trrap

Ensembl Gene

ENSMUSG00000045482

Gene Name

transformation/transcription domain-associated protein

Synonyms

transactivation/transformation-domain associated protein

MMRRC Submission

039923-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1903 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144704547-144796588 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 144752863 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Arginine at position 1813 (I1813R)

Ref Sequence

ENSEMBL: ENSMUSP00000148419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038980] [ENSMUST00000094120] [ENSMUST00000100467] [ENSMUST00000213013]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000038980
AA Change: I1794R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000042544
Gene: ENSMUSG00000045482
AA Change: I1794R

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3363	3376	N/A	INTRINSIC
low complexity region	3407	3418	N/A	INTRINSIC
PI3Kc	3509	3798	5.11e-8	SMART
FATC	3797	3829	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000094120
AA Change: I1812R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000091668
Gene: ENSMUSG00000045482
AA Change: I1812R

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1682	2e-6	SMART
low complexity region	1850	1861	N/A	INTRINSIC
low complexity region	1884	1899	N/A	INTRINSIC
low complexity region	2307	2321	N/A	INTRINSIC
Pfam:FAT	2848	3203	1.1e-68	PFAM
low complexity region	3392	3405	N/A	INTRINSIC
low complexity region	3436	3447	N/A	INTRINSIC
PI3Kc	3538	3827	5.11e-8	SMART
FATC	3826	3858	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000100467
AA Change: I1794R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000098035
Gene: ENSMUSG00000045482
AA Change: I1794R

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3381	3394	N/A	INTRINSIC
low complexity region	3425	3436	N/A	INTRINSIC
PI3Kc	3527	3816	5.11e-8	SMART
FATC	3815	3847	1.89e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132347

Predicted Effect

unknown
Transcript: ENSMUST00000132925
AA Change: I1533R

SMART Domains

Protein: ENSMUSP00000122021
Gene: ENSMUSG00000045482
AA Change: I1533R

Domain	Start	End	E-Value	Type
low complexity region	197	242	N/A	INTRINSIC
low complexity region	244	255	N/A	INTRINSIC
SCOP:d1gw5a_	474	1003	9e-7	SMART
Blast:PI3Kc	480	579	1e-13	BLAST
low complexity region	1083	1092	N/A	INTRINSIC
low complexity region	1572	1583	N/A	INTRINSIC
low complexity region	1606	1621	N/A	INTRINSIC
low complexity region	2029	2043	N/A	INTRINSIC
Pfam:FAT	2570	2914	1.5e-69	PFAM
low complexity region	3121	3134	N/A	INTRINSIC
low complexity region	3165	3176	N/A	INTRINSIC
PI3Kc	3267	3556	5.11e-8	SMART
FATC	3555	3587	1.89e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143357

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197834

Predicted Effect

probably damaging
Transcript: ENSMUST00000213013
AA Change: I1813R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 97.3%
3x: 96.7%
10x: 95.0%
20x: 91.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygous embryos die prior to E3.5 and exhibit embryonic and extraembryonic tissue disorganization. Mitotic abnormalities were also noted in homozygous cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 120 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	A	C	9: 57,165,635 (GRCm39)	S246R	possibly damaging	Het
Abca13	C	T	11: 9,416,411 (GRCm39)	R4058C	probably benign	Het
Acacb	A	T	5: 114,303,795 (GRCm39)	R73*	probably null	Het
Adam22	C	T	5: 8,184,525 (GRCm39)	C489Y	probably damaging	Het
Agap3	A	T	5: 24,698,011 (GRCm39)	K460I	probably damaging	Het
Ak4	T	C	4: 101,320,833 (GRCm39)	I214T	possibly damaging	Het
Anpep	C	T	7: 79,488,004 (GRCm39)	E518K	probably benign	Het
Arrb2	T	A	11: 70,328,808 (GRCm39)	H221Q	probably damaging	Het
Atl1	T	G	12: 70,006,049 (GRCm39)	F452V	probably damaging	Het
Atp8b5	A	G	4: 43,357,063 (GRCm39)	T604A	probably damaging	Het
Bglap3	T	C	3: 88,276,068 (GRCm39)	I95V	probably benign	Het
Ccdc88a	G	T	11: 29,411,788 (GRCm39)	M532I	probably benign	Het
Ccnl1	A	G	3: 65,854,332 (GRCm39)	S430P	possibly damaging	Het
Cdhr18	C	T	14: 13,829,657 (GRCm38)	S695N	possibly damaging	Het
Cdk5rap2	A	T	4: 70,321,791 (GRCm39)		probably null	Het
Cep126	A	T	9: 8,120,748 (GRCm39)	Y92N	possibly damaging	Het
Cfap44	A	C	16: 44,242,737 (GRCm39)	T714P	probably benign	Het
Cnga1	T	G	5: 72,774,068 (GRCm39)	D90A	possibly damaging	Het
Cnot1	T	C	8: 96,469,749 (GRCm39)	I1369V	possibly damaging	Het
Coq3	T	C	4: 21,910,466 (GRCm39)	S314P	probably damaging	Het
Crhr1	A	G	11: 104,060,675 (GRCm39)	R151G	probably damaging	Het
Crybg2	A	G	4: 133,806,167 (GRCm39)	I930V	probably damaging	Het
Ctcf	T	A	8: 106,402,620 (GRCm39)		probably null	Het
Dct	T	C	14: 118,271,690 (GRCm39)	N380S	probably benign	Het
Decr2	A	T	17: 26,306,387 (GRCm39)	L83Q	probably damaging	Het
Depdc5	CTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT	CTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT	5: 33,067,751 (GRCm39)		probably benign	Het
Dgkb	T	C	12: 38,216,776 (GRCm39)		probably null	Het
Dnah1	T	C	14: 31,041,716 (GRCm39)	D85G	probably damaging	Het
Dnah7a	T	C	1: 53,574,637 (GRCm39)	D1709G	probably damaging	Het
Dnajc13	A	C	9: 104,106,136 (GRCm39)	L346R	probably damaging	Het
Dsc1	A	T	18: 20,229,045 (GRCm39)	V415D	probably damaging	Het
Duox2	T	A	2: 122,125,832 (GRCm39)	I296F	probably damaging	Het
Ece2	T	A	16: 20,463,922 (GRCm39)	L890H	probably damaging	Het
Ecsit	A	G	9: 21,987,815 (GRCm39)	S75P	possibly damaging	Het
Enpp3	A	G	10: 24,654,687 (GRCm39)	C664R	probably damaging	Het
Evpl	G	T	11: 116,117,854 (GRCm39)	D778E	probably damaging	Het
Eya3	T	A	4: 132,448,663 (GRCm39)		probably null	Het
Fam217b	A	T	2: 178,062,374 (GRCm39)	I113F	probably benign	Het
Galnt6	G	A	15: 100,613,999 (GRCm39)	P101S	possibly damaging	Het
Gm379	G	A	X: 107,707,870 (GRCm39)	Q210*	probably null	Het
Grk4	A	T	5: 34,833,531 (GRCm39)		probably null	Het
Gtf3c4	A	G	2: 28,729,968 (GRCm39)	V91A	probably benign	Het
Hcfc2	G	T	10: 82,538,392 (GRCm39)	G143V	probably damaging	Het
Heatr4	A	C	12: 84,005,221 (GRCm39)	H710Q	probably damaging	Het
Htr3a	A	T	9: 48,817,681 (GRCm39)	D97E	probably damaging	Het
Htr4	T	G	18: 62,561,193 (GRCm39)	F151L	probably benign	Het
Il22ra1	A	T	4: 135,478,219 (GRCm39)	Q430L	probably damaging	Het
Invs	T	A	4: 48,402,824 (GRCm39)		probably null	Het
Ip6k1	G	A	9: 107,918,195 (GRCm39)	E77K	possibly damaging	Het
Irs1	T	C	1: 82,267,182 (GRCm39)	S345G	probably damaging	Het
Kdm4a	A	G	4: 118,017,596 (GRCm39)	V490A	probably benign	Het
Kif26a	C	T	12: 112,141,974 (GRCm39)	R743C	probably damaging	Het
Kif28	A	G	1: 179,530,088 (GRCm39)	V691A	possibly damaging	Het
Klhl5	T	A	5: 65,324,330 (GRCm39)	L696Q	probably benign	Het
Krtap5-1	T	C	7: 141,850,084 (GRCm39)		probably benign	Het
Lama2	A	C	10: 27,064,395 (GRCm39)	D1195E	probably damaging	Het
Lamb1	T	G	12: 31,379,209 (GRCm39)	L1722R	probably damaging	Het
Lrp11	T	A	10: 7,499,544 (GRCm39)	L245Q	probably damaging	Het
Ltbp2	T	C	12: 84,876,879 (GRCm39)	E422G	probably benign	Het
Man2b1	T	A	8: 85,813,451 (GRCm39)	D214E	probably damaging	Het
Mlxipl	A	T	5: 135,162,422 (GRCm39)	D628V	possibly damaging	Het
Msantd5f6	T	A	4: 73,321,675 (GRCm39)	Y120F	probably damaging	Het
Myo18b	G	A	5: 112,840,624 (GRCm39)	R2390C	probably damaging	Het
Mypn	T	A	10: 62,959,176 (GRCm39)	R1048S	probably benign	Het
Napepld	A	G	5: 21,870,270 (GRCm39)	S383P	probably damaging	Het
Napsa	A	G	7: 44,231,160 (GRCm39)	T130A	probably damaging	Het
Nbr1	T	C	11: 101,465,978 (GRCm39)	I716T	probably damaging	Het
Nexn	A	T	3: 151,953,818 (GRCm39)	M212K	probably damaging	Het
Nlrp9b	T	A	7: 19,757,182 (GRCm39)	S140T	probably benign	Het
Nxpe2	T	C	9: 48,230,906 (GRCm39)	T488A	probably benign	Het
Or10g3	T	G	14: 52,610,174 (GRCm39)	Q112P	possibly damaging	Het
Or10v1	A	T	19: 11,873,913 (GRCm39)	Y176F	probably benign	Het
Or5ac25	A	T	16: 59,182,526 (GRCm39)	D18E	probably benign	Het
Or7g12	A	T	9: 18,900,192 (GRCm39)	K303*	probably null	Het
Or8k20	C	T	2: 86,106,190 (GRCm39)	V214I	probably benign	Het
Osbpl5	T	C	7: 143,256,918 (GRCm39)	D404G	possibly damaging	Het
Pan2	T	G	10: 128,144,237 (GRCm39)	L162R	probably damaging	Het
Parp1	G	T	1: 180,416,235 (GRCm39)	V545F	probably damaging	Het
Pcdh18	A	G	3: 49,709,896 (GRCm39)	V473A	probably benign	Het
Plb1	A	T	5: 32,448,582 (GRCm39)	N350I	probably damaging	Het
Polr1a	A	G	6: 71,944,898 (GRCm39)	K1318R	probably benign	Het
Ppp1r18	C	T	17: 36,184,738 (GRCm39)	P130S	probably damaging	Het
Prss48	C	T	3: 85,905,614 (GRCm39)	W86*	probably null	Het
Rab3c	A	T	13: 110,220,744 (GRCm39)	I137N	probably damaging	Het
Rab3gap2	G	C	1: 184,954,099 (GRCm39)	R57P	probably benign	Het
Rad54l2	A	C	9: 106,570,916 (GRCm39)		probably null	Het
Ralgapb	C	A	2: 158,337,483 (GRCm39)	N1147K	probably benign	Het
Rfx7	C	T	9: 72,524,093 (GRCm39)	R428C	probably damaging	Het
Robo1	A	G	16: 72,757,092 (GRCm39)	Q351R	probably null	Het
Samd4	T	C	14: 47,311,585 (GRCm39)	F81S	probably damaging	Het
Shprh	T	A	10: 11,059,541 (GRCm39)	Y1097*	probably null	Het
Sik3	C	G	9: 46,132,387 (GRCm39)	H1276Q	probably benign	Het
Slc24a4	T	A	12: 102,097,876 (GRCm39)	D79E	probably benign	Het
Slc7a13	A	T	4: 19,839,254 (GRCm39)	I286F	probably benign	Het
Smarca1	G	A	X: 46,938,840 (GRCm39)	Q723*	probably null	Het
Spata31d1b	T	C	13: 59,865,882 (GRCm39)	L1010P	probably damaging	Het
Sult2a1	T	C	7: 13,569,900 (GRCm39)	S111G	possibly damaging	Het
Tecpr2	C	T	12: 110,914,346 (GRCm39)	T1219M	probably damaging	Het
Tesk1	T	C	4: 43,446,998 (GRCm39)	M462T	probably benign	Het
Thoc2l	T	A	5: 104,666,196 (GRCm39)	S239R	probably benign	Het
Tmem171	C	A	13: 98,822,924 (GRCm39)	G292*	probably null	Het
Tmtc2	G	T	10: 105,025,969 (GRCm39)	T833N	probably benign	Het
Tnc	G	T	4: 63,918,299 (GRCm39)	T1204K	probably benign	Het
Tnfaip3	T	C	10: 18,883,937 (GRCm39)	K148E	probably benign	Het
Tnrc18	G	A	5: 142,800,895 (GRCm39)	S21F	probably damaging	Het
Tns4	T	C	11: 98,966,401 (GRCm39)	T425A	probably damaging	Het
Tox	T	A	4: 6,688,948 (GRCm39)	Y472F	probably damaging	Het
Trak2	A	T	1: 58,958,014 (GRCm39)		probably null	Het
Trim33	T	A	3: 103,244,760 (GRCm39)	Y716N	probably damaging	Het
Ttc29	A	G	8: 78,978,361 (GRCm39)	E137G	probably benign	Het
Ube2j2	A	G	4: 156,033,483 (GRCm39)	K19R	probably benign	Het
Ubxn2b	T	C	4: 6,208,889 (GRCm39)	I206T	possibly damaging	Het
Usp40	A	G	1: 87,909,778 (GRCm39)	F559L	probably benign	Het
Utp4	T	A	8: 107,638,982 (GRCm39)		probably null	Het
Vac14	A	G	8: 111,409,166 (GRCm39)	N524S	probably benign	Het
Vps13c	T	C	9: 67,801,334 (GRCm39)	S605P	probably damaging	Het
Vwa5b2	G	T	16: 20,423,582 (GRCm39)	S1165I	possibly damaging	Het
Zdhhc19	C	T	16: 32,317,231 (GRCm39)	R28*	probably null	Het
Zfp106	T	C	2: 120,357,329 (GRCm39)	I1189V	probably benign	Het
Zfp189	C	T	4: 49,529,511 (GRCm39)	Q205*	probably null	Het

Other mutations in Trrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Trrap	APN	5	144,716,784 (GRCm39)	splice site	probably benign
IGL00470:Trrap	APN	5	144,754,848 (GRCm39)	missense	probably damaging	1.00
IGL00490:Trrap	APN	5	144,762,035 (GRCm39)	missense	probably benign	0.40
IGL01072:Trrap	APN	5	144,721,065 (GRCm39)	splice site	probably benign
IGL01087:Trrap	APN	5	144,783,349 (GRCm39)	missense	probably damaging	0.99
IGL01300:Trrap	APN	5	144,741,628 (GRCm39)	missense	probably damaging	1.00
IGL01350:Trrap	APN	5	144,767,779 (GRCm39)	missense	possibly damaging	0.92
IGL01410:Trrap	APN	5	144,767,831 (GRCm39)	missense	probably benign	0.00
IGL01571:Trrap	APN	5	144,770,097 (GRCm39)	splice site	probably benign
IGL01748:Trrap	APN	5	144,770,150 (GRCm39)	missense	probably damaging	1.00
IGL01839:Trrap	APN	5	144,758,685 (GRCm39)	missense	probably damaging	1.00
IGL01976:Trrap	APN	5	144,793,799 (GRCm39)	missense	probably benign	0.00
IGL02075:Trrap	APN	5	144,765,304 (GRCm39)	missense	probably benign	0.00
IGL02127:Trrap	APN	5	144,753,243 (GRCm39)	missense	probably benign	0.22
IGL02131:Trrap	APN	5	144,777,246 (GRCm39)	missense	probably damaging	1.00
IGL02287:Trrap	APN	5	144,769,348 (GRCm39)	missense	probably damaging	1.00
IGL02301:Trrap	APN	5	144,714,727 (GRCm39)	missense	probably benign	0.05
IGL02336:Trrap	APN	5	144,735,200 (GRCm39)	missense	probably benign	0.39
IGL02526:Trrap	APN	5	144,761,360 (GRCm39)	missense	probably benign	0.00
IGL02873:Trrap	APN	5	144,777,889 (GRCm39)	splice site	probably benign
IGL02953:Trrap	APN	5	144,752,774 (GRCm39)	missense	probably damaging	0.99
IGL03404:Trrap	APN	5	144,769,996 (GRCm39)	missense	probably benign	0.00
Buffer	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
Card-tower	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
Cookie	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
Glass_house	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
Immovable	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R5049_trrap_520	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R7167_Trrap_977	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
vitreous	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
PIT4243001:Trrap	UTSW	5	144,733,781 (GRCm39)	missense	probably benign	0.00
PIT4466001:Trrap	UTSW	5	144,765,410 (GRCm39)	missense	probably benign	0.02
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0112:Trrap	UTSW	5	144,759,571 (GRCm39)	nonsense	probably null
R0126:Trrap	UTSW	5	144,742,560 (GRCm39)	nonsense	probably null
R0257:Trrap	UTSW	5	144,741,045 (GRCm39)	missense	probably benign	0.31
R0325:Trrap	UTSW	5	144,753,205 (GRCm39)	missense	probably benign	0.05
R0376:Trrap	UTSW	5	144,753,149 (GRCm39)	missense	probably benign	0.03
R0396:Trrap	UTSW	5	144,751,366 (GRCm39)	missense	probably damaging	0.99
R0448:Trrap	UTSW	5	144,776,377 (GRCm39)	missense	possibly damaging	0.66
R0454:Trrap	UTSW	5	144,783,287 (GRCm39)	missense	probably damaging	1.00
R0711:Trrap	UTSW	5	144,790,309 (GRCm39)	missense	probably damaging	1.00
R0827:Trrap	UTSW	5	144,751,640 (GRCm39)	missense	probably benign	0.00
R1005:Trrap	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1179:Trrap	UTSW	5	144,714,749 (GRCm39)	missense	possibly damaging	0.94
R1218:Trrap	UTSW	5	144,753,219 (GRCm39)	missense	probably damaging	1.00
R1264:Trrap	UTSW	5	144,726,409 (GRCm39)	splice site	probably benign
R1374:Trrap	UTSW	5	144,783,428 (GRCm39)	missense	probably damaging	1.00
R1401:Trrap	UTSW	5	144,794,232 (GRCm39)	missense	possibly damaging	0.93
R1480:Trrap	UTSW	5	144,755,123 (GRCm39)	missense	probably benign
R1538:Trrap	UTSW	5	144,774,012 (GRCm39)	missense	possibly damaging	0.65
R1751:Trrap	UTSW	5	144,751,385 (GRCm39)	critical splice donor site	probably null
R1779:Trrap	UTSW	5	144,765,400 (GRCm39)	missense	probably benign	0.01
R1782:Trrap	UTSW	5	144,759,513 (GRCm39)	missense	possibly damaging	0.93
R1792:Trrap	UTSW	5	144,790,396 (GRCm39)	missense	possibly damaging	0.87
R1859:Trrap	UTSW	5	144,767,761 (GRCm39)	missense	probably benign	0.04
R1861:Trrap	UTSW	5	144,752,727 (GRCm39)	splice site	probably null
R1902:Trrap	UTSW	5	144,752,863 (GRCm39)	missense	probably damaging	1.00
R2021:Trrap	UTSW	5	144,790,298 (GRCm39)	missense	possibly damaging	0.94
R2026:Trrap	UTSW	5	144,739,854 (GRCm39)	missense	possibly damaging	0.86
R2036:Trrap	UTSW	5	144,765,372 (GRCm39)	missense	probably benign	0.08
R2099:Trrap	UTSW	5	144,719,049 (GRCm39)	missense	possibly damaging	0.46
R2108:Trrap	UTSW	5	144,762,684 (GRCm39)	missense	probably benign	0.01
R2113:Trrap	UTSW	5	144,781,021 (GRCm39)	missense	probably damaging	1.00
R2174:Trrap	UTSW	5	144,758,665 (GRCm39)	missense	probably benign	0.40
R2442:Trrap	UTSW	5	144,754,776 (GRCm39)	missense	probably damaging	1.00
R2568:Trrap	UTSW	5	144,780,179 (GRCm39)	critical splice donor site	probably null
R3442:Trrap	UTSW	5	144,729,062 (GRCm39)	missense	probably benign	0.03
R3853:Trrap	UTSW	5	144,728,975 (GRCm39)	missense	probably damaging	1.00
R4401:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R4493:Trrap	UTSW	5	144,767,858 (GRCm39)	missense	probably benign	0.21
R4524:Trrap	UTSW	5	144,762,131 (GRCm39)	missense	probably benign	0.38
R4569:Trrap	UTSW	5	144,728,928 (GRCm39)	missense	probably benign	0.13
R4672:Trrap	UTSW	5	144,722,290 (GRCm39)	missense	probably damaging	0.97
R4732:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4733:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4791:Trrap	UTSW	5	144,740,087 (GRCm39)	missense	probably damaging	1.00
R4795:Trrap	UTSW	5	144,769,298 (GRCm39)	missense	probably benign	0.06
R4827:Trrap	UTSW	5	144,737,758 (GRCm39)	missense	probably benign	0.02
R4839:Trrap	UTSW	5	144,782,402 (GRCm39)	missense	probably damaging	1.00
R4915:Trrap	UTSW	5	144,742,545 (GRCm39)	missense	probably damaging	0.99
R4951:Trrap	UTSW	5	144,742,530 (GRCm39)	missense	possibly damaging	0.65
R4959:Trrap	UTSW	5	144,793,770 (GRCm39)	missense	probably damaging	1.00
R5049:Trrap	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R5074:Trrap	UTSW	5	144,787,989 (GRCm39)	missense	probably damaging	1.00
R5236:Trrap	UTSW	5	144,754,596 (GRCm39)	missense	probably benign	0.07
R5281:Trrap	UTSW	5	144,750,313 (GRCm39)	missense	probably benign	0.13
R5322:Trrap	UTSW	5	144,781,034 (GRCm39)	missense	probably damaging	1.00
R5457:Trrap	UTSW	5	144,786,787 (GRCm39)	missense	probably damaging	1.00
R5590:Trrap	UTSW	5	144,719,075 (GRCm39)	missense	probably benign	0.05
R5799:Trrap	UTSW	5	144,767,755 (GRCm39)	missense	probably benign
R5885:Trrap	UTSW	5	144,731,603 (GRCm39)	missense	probably damaging	1.00
R5905:Trrap	UTSW	5	144,786,730 (GRCm39)	missense	possibly damaging	0.95
R5908:Trrap	UTSW	5	144,723,518 (GRCm39)	missense	probably damaging	0.96
R5956:Trrap	UTSW	5	144,744,201 (GRCm39)	splice site	silent
R5992:Trrap	UTSW	5	144,746,994 (GRCm39)	missense	probably benign	0.00
R6017:Trrap	UTSW	5	144,781,051 (GRCm39)	missense	probably damaging	1.00
R6029:Trrap	UTSW	5	144,762,724 (GRCm39)	missense	possibly damaging	0.75
R6029:Trrap	UTSW	5	144,754,489 (GRCm39)	missense	possibly damaging	0.94
R6117:Trrap	UTSW	5	144,739,771 (GRCm39)	missense	possibly damaging	0.78
R6166:Trrap	UTSW	5	144,718,791 (GRCm39)	missense	possibly damaging	0.66
R6234:Trrap	UTSW	5	144,776,523 (GRCm39)	splice site	probably null
R6288:Trrap	UTSW	5	144,748,802 (GRCm39)	missense	probably damaging	1.00
R6290:Trrap	UTSW	5	144,741,828 (GRCm39)	missense	probably damaging	1.00
R6316:Trrap	UTSW	5	144,750,336 (GRCm39)	missense	probably benign	0.02
R6398:Trrap	UTSW	5	144,727,680 (GRCm39)	missense	possibly damaging	0.83
R6413:Trrap	UTSW	5	144,720,856 (GRCm39)	missense	possibly damaging	0.83
R6499:Trrap	UTSW	5	144,793,812 (GRCm39)	missense	probably damaging	1.00
R6529:Trrap	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
R6574:Trrap	UTSW	5	144,752,360 (GRCm39)	critical splice donor site	probably null
R6631:Trrap	UTSW	5	144,708,460 (GRCm39)	missense	possibly damaging	0.94
R6727:Trrap	UTSW	5	144,793,760 (GRCm39)	missense	probably damaging	1.00
R6776:Trrap	UTSW	5	144,788,066 (GRCm39)	nonsense	probably null
R6914:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6942:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6945:Trrap	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R7023:Trrap	UTSW	5	144,728,964 (GRCm39)	missense	possibly damaging	0.64
R7107:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7139:Trrap	UTSW	5	144,739,988 (GRCm39)	missense	possibly damaging	0.65
R7148:Trrap	UTSW	5	144,758,613 (GRCm39)	missense	possibly damaging	0.77
R7167:Trrap	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
R7171:Trrap	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
R7205:Trrap	UTSW	5	144,779,517 (GRCm39)	missense	possibly damaging	0.94
R7215:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7255:Trrap	UTSW	5	144,795,764 (GRCm39)	missense	probably damaging	1.00
R7261:Trrap	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
R7264:Trrap	UTSW	5	144,751,333 (GRCm39)	missense	probably benign	0.05
R7372:Trrap	UTSW	5	144,726,208 (GRCm39)	missense	probably benign
R7447:Trrap	UTSW	5	144,776,284 (GRCm39)	missense	probably damaging	0.97
R7449:Trrap	UTSW	5	144,788,019 (GRCm39)	missense	probably damaging	1.00
R7655:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7656:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7662:Trrap	UTSW	5	144,769,321 (GRCm39)	missense	probably benign	0.00
R7716:Trrap	UTSW	5	144,713,956 (GRCm39)	missense	possibly damaging	0.73
R8143:Trrap	UTSW	5	144,772,707 (GRCm39)	splice site	probably null
R8183:Trrap	UTSW	5	144,765,343 (GRCm39)	missense	probably benign	0.01
R8265:Trrap	UTSW	5	144,722,344 (GRCm39)	missense	possibly damaging	0.53
R8273:Trrap	UTSW	5	144,727,975 (GRCm39)	missense	probably damaging	1.00
R8556:Trrap	UTSW	5	144,762,747 (GRCm39)	missense	probably benign	0.44
R8674:Trrap	UTSW	5	144,727,842 (GRCm39)	missense	probably benign	0.02
R8777:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8777-TAIL:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8817:Trrap	UTSW	5	144,782,348 (GRCm39)	missense	probably damaging	1.00
R8841:Trrap	UTSW	5	144,781,021 (GRCm39)	missense	probably damaging	1.00
R8871:Trrap	UTSW	5	144,758,649 (GRCm39)	missense	probably benign	0.30
R8937:Trrap	UTSW	5	144,757,063 (GRCm39)	missense	probably damaging	1.00
R8966:Trrap	UTSW	5	144,740,162 (GRCm39)	missense	probably damaging	0.96
R9010:Trrap	UTSW	5	144,783,226 (GRCm39)	missense	probably damaging	1.00
R9095:Trrap	UTSW	5	144,733,961 (GRCm39)	missense	probably damaging	1.00
R9127:Trrap	UTSW	5	144,767,830 (GRCm39)	missense	probably benign	0.16
R9132:Trrap	UTSW	5	144,726,362 (GRCm39)	missense	probably benign	0.03
R9224:Trrap	UTSW	5	144,708,049 (GRCm39)	missense	possibly damaging	0.70
R9338:Trrap	UTSW	5	144,727,925 (GRCm39)	missense	probably benign
R9380:Trrap	UTSW	5	144,769,981 (GRCm39)	missense	probably benign
R9404:Trrap	UTSW	5	144,752,225 (GRCm39)	missense	possibly damaging	0.85
R9457:Trrap	UTSW	5	144,763,478 (GRCm39)	missense	probably damaging	1.00
R9464:Trrap	UTSW	5	144,763,517 (GRCm39)	missense	probably damaging	0.99
R9504:Trrap	UTSW	5	144,742,904 (GRCm39)	missense	probably damaging	1.00
R9583:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9584:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9585:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9608:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R9728:Trrap	UTSW	5	144,726,193 (GRCm39)	missense	probably benign	0.22
R9782:Trrap	UTSW	5	144,758,716 (GRCm39)	missense	probably damaging	0.99
X0060:Trrap	UTSW	5	144,780,171 (GRCm39)	missense	probably damaging	0.96
Z1088:Trrap	UTSW	5	144,771,007 (GRCm39)	missense	probably benign	0.00
Z1177:Trrap	UTSW	5	144,756,518 (GRCm39)	missense	probably damaging	1.00
Z1177:Trrap	UTSW	5	144,747,154 (GRCm39)	missense
Z1177:Trrap	UTSW	5	144,793,761 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCGTTCATTCATGCACAGC -3'
(R):5'- TTGTTTCTCAGGGTCCAGGAC -3'

Sequencing Primer
(F):5'- ATTCATGCACAGCTCTGTTCTG -3'
(R):5'- GGGGCCCAAGGAAAATATTA -3'

Posted On

2014-06-30