Mutagenetix > Incidental Mutation

Incidental Mutation 'R1430:Zfp879'

161492

Institutional Source

Beutler Lab

Gene Symbol

Zfp879

Ensembl Gene

ENSMUSG00000044296

Gene Name

zinc finger protein 879

Synonyms

9630041N07Rik

MMRRC Submission

039486-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1430 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50722858-50732379 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 50724784 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 91 (F91L)

Ref Sequence

ENSEMBL: ENSMUSP00000104762 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000049625] [ENSMUST00000109133] [ENSMUST00000109134]

AlphaFold

Q8BI99

Predicted Effect

probably benign
Transcript: ENSMUST00000049625
AA Change: F91L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000061782
Gene: ENSMUSG00000044296
AA Change: F91L

Domain	Start	End	E-Value	Type
KRAB	14	74	1.54e-33	SMART
ZnF_C2H2	204	226	1.92e-2	SMART
ZnF_C2H2	232	254	1.38e-3	SMART
ZnF_C2H2	260	282	1.16e-1	SMART
ZnF_C2H2	288	310	4.54e-4	SMART
ZnF_C2H2	316	338	8.34e-3	SMART
ZnF_C2H2	344	366	4.87e-4	SMART
ZnF_C2H2	372	394	8.47e-4	SMART
ZnF_C2H2	400	422	1.84e-4	SMART
ZnF_C2H2	428	450	2.57e-3	SMART
ZnF_C2H2	456	478	1.47e-3	SMART
ZnF_C2H2	484	506	3.21e-4	SMART
ZnF_C2H2	512	534	1.5e-4	SMART
ZnF_C2H2	540	562	5.21e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109133
AA Change: F18L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000104761
Gene: ENSMUSG00000044296
AA Change: F18L

Domain	Start	End	E-Value	Type
ZnF_C2H2	131	153	1.92e-2	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	1.16e-1	SMART
ZnF_C2H2	215	237	4.54e-4	SMART
ZnF_C2H2	243	265	8.34e-3	SMART
ZnF_C2H2	271	293	4.87e-4	SMART
ZnF_C2H2	299	321	8.47e-4	SMART
ZnF_C2H2	327	349	1.84e-4	SMART
ZnF_C2H2	355	377	2.57e-3	SMART
ZnF_C2H2	383	405	1.47e-3	SMART
ZnF_C2H2	411	433	3.21e-4	SMART
ZnF_C2H2	439	461	1.5e-4	SMART
ZnF_C2H2	467	489	5.21e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109134
AA Change: F91L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000104762
Gene: ENSMUSG00000044296
AA Change: F91L

Domain	Start	End	E-Value	Type
KRAB	14	74	1.54e-33	SMART
ZnF_C2H2	204	226	1.92e-2	SMART
ZnF_C2H2	232	254	1.38e-3	SMART
ZnF_C2H2	260	282	1.16e-1	SMART
ZnF_C2H2	288	310	4.54e-4	SMART
ZnF_C2H2	316	338	8.34e-3	SMART
ZnF_C2H2	344	366	4.87e-4	SMART
ZnF_C2H2	372	394	8.47e-4	SMART
ZnF_C2H2	400	422	1.84e-4	SMART
ZnF_C2H2	428	450	2.57e-3	SMART
ZnF_C2H2	456	478	1.47e-3	SMART
ZnF_C2H2	484	506	3.21e-4	SMART
ZnF_C2H2	512	534	1.5e-4	SMART
ZnF_C2H2	540	562	5.21e-4	SMART

Meta Mutation Damage Score

0.0600

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 89.9%

Validation Efficiency

100% (55/55)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam3	T	A	8: 25,204,287 (GRCm39)		probably benign	Het
Aoc2	A	G	11: 101,217,321 (GRCm39)	Y468C	probably damaging	Het
Cdyl2	A	G	8: 117,306,056 (GRCm39)		probably benign	Het
Cfh	A	G	1: 140,030,436 (GRCm39)		probably benign	Het
Cyp2j9	G	T	4: 96,472,201 (GRCm39)		probably benign	Het
Dapk1	T	G	13: 60,901,957 (GRCm39)	F929V	probably benign	Het
Dhx9	A	T	1: 153,359,493 (GRCm39)	M35K	probably benign	Het
Dnah5	G	A	15: 28,346,003 (GRCm39)	E2448K	probably benign	Het
Doc2b	A	T	11: 75,670,981 (GRCm39)	C217S	possibly damaging	Het
Dock11	A	G	X: 35,333,565 (GRCm39)	I2010V	probably benign	Het
Dram1	T	C	10: 88,160,641 (GRCm39)	T227A	possibly damaging	Het
Eppin	G	A	2: 164,431,323 (GRCm39)	T101M	probably damaging	Het
F13a1	T	C	13: 37,082,105 (GRCm39)	D533G	probably damaging	Het
Fmo1	C	A	1: 162,667,293 (GRCm39)	R174L	probably damaging	Het
Fsip2	C	A	2: 82,828,407 (GRCm39)	L6735I	possibly damaging	Het
Gab1	G	T	8: 81,515,241 (GRCm39)	T359K	probably benign	Het
Ggta1	T	C	2: 35,298,029 (GRCm39)	D118G	possibly damaging	Het
Gramd1a	A	G	7: 30,832,211 (GRCm39)	S609P	probably damaging	Het
Gtf3c3	C	T	1: 54,456,937 (GRCm39)	A488T	probably damaging	Het
Hsh2d	G	A	8: 72,954,304 (GRCm39)	D229N	probably benign	Het
Lama1	A	G	17: 68,089,150 (GRCm39)	Y1607C	possibly damaging	Het
Lrrc24	G	T	15: 76,607,992 (GRCm39)		probably null	Het
Mak	C	A	13: 41,223,760 (GRCm39)		probably benign	Het
Megf8	G	A	7: 25,063,768 (GRCm39)	R2708Q	possibly damaging	Het
Mettl24	T	C	10: 40,613,791 (GRCm39)	C177R	probably damaging	Het
Mgam	G	C	6: 40,733,305 (GRCm39)	E812D	probably benign	Het
Mroh8	T	C	2: 157,111,445 (GRCm39)	R170G	possibly damaging	Het
Msn	G	A	X: 95,196,325 (GRCm39)	V130I	probably benign	Het
Ncoa4	G	A	14: 31,898,679 (GRCm39)	V500I	probably benign	Het
Or2aj5	A	G	16: 19,424,752 (GRCm39)	L222P	probably damaging	Het
Or2y1b	A	G	11: 49,208,928 (GRCm39)		probably null	Het
Or5an6	C	T	19: 12,371,801 (GRCm39)	T58I	probably benign	Het
Or8k3b	T	C	2: 86,520,866 (GRCm39)	Y151C	possibly damaging	Het
Ppm1h	T	C	10: 122,693,004 (GRCm39)	S302P	probably damaging	Het
Prkce	T	A	17: 86,866,565 (GRCm39)		probably benign	Het
Psenen	T	C	7: 30,261,815 (GRCm39)	I34V	probably benign	Het
Rbl1	T	C	2: 157,011,826 (GRCm39)	T710A	probably benign	Het
Sdk2	C	T	11: 113,729,472 (GRCm39)		silent	Het
Slc1a5	A	G	7: 16,516,328 (GRCm39)	D168G	probably benign	Het
Slc35e1	T	C	8: 73,246,415 (GRCm39)		probably benign	Het
Sned1	G	A	1: 93,209,376 (GRCm39)	V830M	possibly damaging	Het
Syce1	A	T	7: 140,359,351 (GRCm39)		probably benign	Het
Tbc1d23	G	T	16: 57,034,573 (GRCm39)	D75E	probably damaging	Het
Tbk1	A	G	10: 121,395,839 (GRCm39)	V418A	probably benign	Het
Tmem132e	T	C	11: 82,329,122 (GRCm39)	V467A	probably damaging	Het
Tmem241	T	C	18: 12,126,651 (GRCm39)	D144G	probably benign	Het
Tsc2	A	G	17: 24,817,997 (GRCm39)		probably null	Het
Ubxn4	T	A	1: 128,202,617 (GRCm39)	F420I	probably benign	Het
Usp34	A	G	11: 23,409,151 (GRCm39)	T2645A	probably damaging	Het
Utp14b	A	G	1: 78,644,111 (GRCm39)	K670E	probably benign	Het
Zfp407	C	T	18: 84,227,580 (GRCm39)	V2010M	probably benign	Het
Zfyve26	A	G	12: 79,329,591 (GRCm39)	S532P	probably benign	Het

Other mutations in Zfp879

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01659:Zfp879	APN	11	50,729,281 (GRCm39)	missense	probably damaging	1.00
IGL02175:Zfp879	APN	11	50,728,743 (GRCm39)	missense	probably benign	0.13
IGL02259:Zfp879	APN	11	50,729,255 (GRCm39)	missense	probably benign	0.00
Bazooka	UTSW	11	50,729,302 (GRCm39)	missense	probably damaging	0.98
joe	UTSW	11	50,724,222 (GRCm39)	missense	probably damaging	0.99
R0131:Zfp879	UTSW	11	50,724,426 (GRCm39)	missense	probably damaging	1.00
R1576:Zfp879	UTSW	11	50,724,376 (GRCm39)	missense	probably benign	0.41
R1616:Zfp879	UTSW	11	50,723,473 (GRCm39)	missense	probably benign	0.06
R1701:Zfp879	UTSW	11	50,724,060 (GRCm39)	missense	possibly damaging	0.77
R1965:Zfp879	UTSW	11	50,724,355 (GRCm39)	missense	probably damaging	1.00
R2057:Zfp879	UTSW	11	50,723,428 (GRCm39)	missense	probably benign
R2058:Zfp879	UTSW	11	50,723,428 (GRCm39)	missense	probably benign
R2219:Zfp879	UTSW	11	50,724,094 (GRCm39)	missense	probably damaging	1.00
R3110:Zfp879	UTSW	11	50,723,989 (GRCm39)	missense	possibly damaging	0.87
R3112:Zfp879	UTSW	11	50,723,989 (GRCm39)	missense	possibly damaging	0.87
R4658:Zfp879	UTSW	11	50,724,024 (GRCm39)	missense	probably damaging	1.00
R4845:Zfp879	UTSW	11	50,724,672 (GRCm39)	missense	probably damaging	1.00
R4998:Zfp879	UTSW	11	50,728,796 (GRCm39)	missense	probably damaging	1.00
R6362:Zfp879	UTSW	11	50,729,302 (GRCm39)	missense	probably damaging	0.98
R6930:Zfp879	UTSW	11	50,723,839 (GRCm39)	missense	probably damaging	1.00
R7091:Zfp879	UTSW	11	50,724,222 (GRCm39)	missense	probably damaging	0.99
R7186:Zfp879	UTSW	11	50,724,621 (GRCm39)	missense	probably benign	0.06
R7218:Zfp879	UTSW	11	50,723,508 (GRCm39)	missense	possibly damaging	0.61
R8138:Zfp879	UTSW	11	50,724,275 (GRCm39)	nonsense	probably null
R8445:Zfp879	UTSW	11	50,724,213 (GRCm39)	missense	probably damaging	0.98
R8523:Zfp879	UTSW	11	50,728,757 (GRCm39)	missense	probably benign
R8725:Zfp879	UTSW	11	50,729,321 (GRCm39)	missense	probably damaging	0.98
R8790:Zfp879	UTSW	11	50,723,429 (GRCm39)	nonsense	probably null
R8882:Zfp879	UTSW	11	50,724,763 (GRCm39)	nonsense	probably null
R9229:Zfp879	UTSW	11	50,723,886 (GRCm39)	missense	probably damaging	1.00
X0066:Zfp879	UTSW	11	50,723,914 (GRCm39)	missense	possibly damaging	0.89
Z1177:Zfp879	UTSW	11	50,724,258 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAGAAAGCAGCATCTCGCTGAAA -3'
(R):5'- GGTACACACAGACACGTAGGCACA -3'

Sequencing Primer
(F):5'- CGCTGAAAGGGTTTTTCTTATTGC -3'
(R):5'- acacacacacacacacacac -3'

Posted On

2014-03-14