Mutagenetix > Incidental Mutation

Incidental Mutation 'R6930:Zfp879'

539968

Institutional Source

Beutler Lab

Gene Symbol

Zfp879

Ensembl Gene

ENSMUSG00000044296

Gene Name

zinc finger protein 879

Synonyms

9630041N07Rik

MMRRC Submission

045046-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6930 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

50722858-50732379 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 50723839 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 406 (G406R)

Ref Sequence

ENSEMBL: ENSMUSP00000104762 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000049625] [ENSMUST00000109133] [ENSMUST00000109134]

AlphaFold

Q8BI99

Predicted Effect

probably damaging
Transcript: ENSMUST00000049625
AA Change: G406R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000061782
Gene: ENSMUSG00000044296
AA Change: G406R

Domain	Start	End	E-Value	Type
KRAB	14	74	1.54e-33	SMART
ZnF_C2H2	204	226	1.92e-2	SMART
ZnF_C2H2	232	254	1.38e-3	SMART
ZnF_C2H2	260	282	1.16e-1	SMART
ZnF_C2H2	288	310	4.54e-4	SMART
ZnF_C2H2	316	338	8.34e-3	SMART
ZnF_C2H2	344	366	4.87e-4	SMART
ZnF_C2H2	372	394	8.47e-4	SMART
ZnF_C2H2	400	422	1.84e-4	SMART
ZnF_C2H2	428	450	2.57e-3	SMART
ZnF_C2H2	456	478	1.47e-3	SMART
ZnF_C2H2	484	506	3.21e-4	SMART
ZnF_C2H2	512	534	1.5e-4	SMART
ZnF_C2H2	540	562	5.21e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109133
AA Change: G333R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000104761
Gene: ENSMUSG00000044296
AA Change: G333R

Domain	Start	End	E-Value	Type
ZnF_C2H2	131	153	1.92e-2	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	1.16e-1	SMART
ZnF_C2H2	215	237	4.54e-4	SMART
ZnF_C2H2	243	265	8.34e-3	SMART
ZnF_C2H2	271	293	4.87e-4	SMART
ZnF_C2H2	299	321	8.47e-4	SMART
ZnF_C2H2	327	349	1.84e-4	SMART
ZnF_C2H2	355	377	2.57e-3	SMART
ZnF_C2H2	383	405	1.47e-3	SMART
ZnF_C2H2	411	433	3.21e-4	SMART
ZnF_C2H2	439	461	1.5e-4	SMART
ZnF_C2H2	467	489	5.21e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109134
AA Change: G406R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000104762
Gene: ENSMUSG00000044296
AA Change: G406R

Domain	Start	End	E-Value	Type
KRAB	14	74	1.54e-33	SMART
ZnF_C2H2	204	226	1.92e-2	SMART
ZnF_C2H2	232	254	1.38e-3	SMART
ZnF_C2H2	260	282	1.16e-1	SMART
ZnF_C2H2	288	310	4.54e-4	SMART
ZnF_C2H2	316	338	8.34e-3	SMART
ZnF_C2H2	344	366	4.87e-4	SMART
ZnF_C2H2	372	394	8.47e-4	SMART
ZnF_C2H2	400	422	1.84e-4	SMART
ZnF_C2H2	428	450	2.57e-3	SMART
ZnF_C2H2	456	478	1.47e-3	SMART
ZnF_C2H2	484	506	3.21e-4	SMART
ZnF_C2H2	512	534	1.5e-4	SMART
ZnF_C2H2	540	562	5.21e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 99.0%
20x: 96.5%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700093K21Rik	A	T	11: 23,466,563 (GRCm39)	D156E	probably benign	Het
Adam17	A	T	12: 21,403,949 (GRCm39)	V99E	probably damaging	Het
Akr1e1	T	C	13: 4,652,714 (GRCm39)	D41G	probably damaging	Het
Alcam	T	C	16: 52,126,018 (GRCm39)	I100V	probably benign	Het
Atr	T	C	9: 95,748,688 (GRCm39)	I411T	probably benign	Het
Bbs4	T	C	9: 59,230,764 (GRCm39)	S453G	probably benign	Het
Brdt	C	T	5: 107,507,081 (GRCm39)	L494F	probably benign	Het
Ccser1	T	C	6: 62,357,009 (GRCm39)	S816P	probably benign	Het
Cdk10	T	C	8: 123,957,347 (GRCm39)	I157T	probably damaging	Het
Ceacam5	G	A	7: 17,484,759 (GRCm39)		probably null	Het
Chst15	T	C	7: 131,870,759 (GRCm39)	I259V	possibly damaging	Het
Cracdl	A	T	1: 37,664,026 (GRCm39)	I624N	possibly damaging	Het
Csmd1	A	T	8: 16,142,409 (GRCm39)	M1498K	probably damaging	Het
D630045J12Rik	T	C	6: 38,135,151 (GRCm39)	D1343G	probably damaging	Het
Denr	T	C	5: 124,046,250 (GRCm39)	Y27H	probably benign	Het
Dop1a	T	C	9: 86,413,825 (GRCm39)		probably null	Het
Epg5	T	C	18: 78,057,378 (GRCm39)	F1819S	probably damaging	Het
Flg2	T	A	3: 93,108,642 (GRCm39)	Y223*	probably null	Het
Fry	T	C	5: 150,351,695 (GRCm39)	L1733P	probably benign	Het
Gabrb2	T	C	11: 42,488,440 (GRCm39)	V302A	probably damaging	Het
Gimap9	G	A	6: 48,654,601 (GRCm39)	D53N	probably damaging	Het
Gje1	G	T	10: 14,593,886 (GRCm39)	L3I	possibly damaging	Het
Gm49383	G	T	12: 69,239,586 (GRCm39)	A645E	probably damaging	Het
Gm8947	G	A	1: 151,068,347 (GRCm39)	G60D	probably damaging	Het
Gpatch2l	G	A	12: 86,290,958 (GRCm39)	R47H	probably damaging	Het
Gys2	A	G	6: 142,405,106 (GRCm39)		probably null	Het
Hace1	A	G	10: 45,494,598 (GRCm39)	H136R	probably damaging	Het
Herc3	T	G	6: 58,893,444 (GRCm39)	V902G	probably damaging	Het
Hspbp1	T	C	7: 4,687,606 (GRCm39)	R2G	probably benign	Het
Iqch	T	A	9: 63,387,856 (GRCm39)	K811N	possibly damaging	Het
Kmt2a	A	G	9: 44,753,962 (GRCm39)		probably benign	Het
Lonrf2	A	T	1: 38,843,417 (GRCm39)	V372D	probably benign	Het
Lpin2	T	C	17: 71,551,786 (GRCm39)	Y729H	probably damaging	Het
Lrrc32	A	G	7: 98,148,471 (GRCm39)	N417S	possibly damaging	Het
Malrd1	T	A	2: 15,802,478 (GRCm39)	C1064S	unknown	Het
Mast3	G	A	8: 71,252,115 (GRCm39)	R20*	probably null	Het
Mypn	A	C	10: 62,952,718 (GRCm39)	I174S	probably damaging	Het
Nrg1	G	A	8: 32,308,534 (GRCm39)	T505M	probably damaging	Het
Or13p8	A	G	4: 118,584,338 (GRCm39)	K298R	probably damaging	Het
Or6y1	A	G	1: 174,276,677 (GRCm39)	I163V	probably damaging	Het
Or7a42	A	G	10: 78,791,615 (GRCm39)	D192G	possibly damaging	Het
Or7e178	A	G	9: 20,225,395 (GRCm39)	Y266H	probably damaging	Het
Phf3	T	C	1: 30,850,958 (GRCm39)	E1132G	probably damaging	Het
Pla2g4d	A	T	2: 120,101,114 (GRCm39)	M521K	probably damaging	Het
Plekhg1	A	G	10: 3,913,770 (GRCm39)	H1164R	possibly damaging	Het
Plxnb2	A	G	15: 89,044,592 (GRCm39)	V1218A	probably benign	Het
Pold1	A	G	7: 44,191,630 (GRCm39)	S119P	probably benign	Het
Pole	T	A	5: 110,441,156 (GRCm39)	D203E	probably benign	Het
Rapgefl1	T	A	11: 98,737,947 (GRCm39)	L387Q	probably damaging	Het
Rbm33	T	C	5: 28,557,504 (GRCm39)	I199T	probably benign	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Rufy1	C	A	11: 50,289,207 (GRCm39)	R545L	probably benign	Het
Ryr3	T	A	2: 112,690,699 (GRCm39)	D1117V	probably damaging	Het
Sap130	T	C	18: 31,815,141 (GRCm39)	V621A	possibly damaging	Het
Sparcl1	T	A	5: 104,234,940 (GRCm39)	Y525F	probably damaging	Het
Spon2	A	G	5: 33,373,771 (GRCm39)	V180A	probably benign	Het
Trav10n	G	A	14: 53,359,947 (GRCm39)	V75M	probably benign	Het
Ttc34	T	C	4: 154,923,543 (GRCm39)	L84P	probably damaging	Het
Vmn1r23	C	T	6: 57,903,130 (GRCm39)	R216K	probably benign	Het
Vmn2r61	A	G	7: 41,949,364 (GRCm39)	T595A	probably benign	Het
Vmn2r66	T	A	7: 84,661,216 (GRCm39)	I5F	possibly damaging	Het
Zic2	A	T	14: 122,713,869 (GRCm39)	D261V	probably damaging	Het

Other mutations in Zfp879

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01659:Zfp879	APN	11	50,729,281 (GRCm39)	missense	probably damaging	1.00
IGL02175:Zfp879	APN	11	50,728,743 (GRCm39)	missense	probably benign	0.13
IGL02259:Zfp879	APN	11	50,729,255 (GRCm39)	missense	probably benign	0.00
Bazooka	UTSW	11	50,729,302 (GRCm39)	missense	probably damaging	0.98
joe	UTSW	11	50,724,222 (GRCm39)	missense	probably damaging	0.99
R0131:Zfp879	UTSW	11	50,724,426 (GRCm39)	missense	probably damaging	1.00
R1430:Zfp879	UTSW	11	50,724,784 (GRCm39)	missense	probably benign	0.00
R1576:Zfp879	UTSW	11	50,724,376 (GRCm39)	missense	probably benign	0.41
R1616:Zfp879	UTSW	11	50,723,473 (GRCm39)	missense	probably benign	0.06
R1701:Zfp879	UTSW	11	50,724,060 (GRCm39)	missense	possibly damaging	0.77
R1965:Zfp879	UTSW	11	50,724,355 (GRCm39)	missense	probably damaging	1.00
R2057:Zfp879	UTSW	11	50,723,428 (GRCm39)	missense	probably benign
R2058:Zfp879	UTSW	11	50,723,428 (GRCm39)	missense	probably benign
R2219:Zfp879	UTSW	11	50,724,094 (GRCm39)	missense	probably damaging	1.00
R3110:Zfp879	UTSW	11	50,723,989 (GRCm39)	missense	possibly damaging	0.87
R3112:Zfp879	UTSW	11	50,723,989 (GRCm39)	missense	possibly damaging	0.87
R4658:Zfp879	UTSW	11	50,724,024 (GRCm39)	missense	probably damaging	1.00
R4845:Zfp879	UTSW	11	50,724,672 (GRCm39)	missense	probably damaging	1.00
R4998:Zfp879	UTSW	11	50,728,796 (GRCm39)	missense	probably damaging	1.00
R6362:Zfp879	UTSW	11	50,729,302 (GRCm39)	missense	probably damaging	0.98
R7091:Zfp879	UTSW	11	50,724,222 (GRCm39)	missense	probably damaging	0.99
R7186:Zfp879	UTSW	11	50,724,621 (GRCm39)	missense	probably benign	0.06
R7218:Zfp879	UTSW	11	50,723,508 (GRCm39)	missense	possibly damaging	0.61
R8138:Zfp879	UTSW	11	50,724,275 (GRCm39)	nonsense	probably null
R8445:Zfp879	UTSW	11	50,724,213 (GRCm39)	missense	probably damaging	0.98
R8523:Zfp879	UTSW	11	50,728,757 (GRCm39)	missense	probably benign
R8725:Zfp879	UTSW	11	50,729,321 (GRCm39)	missense	probably damaging	0.98
R8790:Zfp879	UTSW	11	50,723,429 (GRCm39)	nonsense	probably null
R8882:Zfp879	UTSW	11	50,724,763 (GRCm39)	nonsense	probably null
R9229:Zfp879	UTSW	11	50,723,886 (GRCm39)	missense	probably damaging	1.00
X0066:Zfp879	UTSW	11	50,723,914 (GRCm39)	missense	possibly damaging	0.89
Z1177:Zfp879	UTSW	11	50,724,258 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACGCTTTCCCGCAAGCT -3'
(R):5'- CACCACCTCTCTCATTGGG -3'

Sequencing Primer
(F):5'- CCTCATATGTGTCATAAGCGAGG -3'
(R):5'- CCATATGAATGCAGCCAGTGTG -3'

Posted On

2018-11-06