Incidental Mutation 'R2355:Mettl25'
| ID |
246880 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mettl25
|
| Ensembl Gene |
ENSMUSG00000036009 |
| Gene Name |
methyltransferase like 25 |
| Synonyms |
BC067068 |
| MMRRC Submission |
040337-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.114)
|
| Stock # |
R2355 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
10 |
| Chromosomal Location |
105599050-105677241 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to A
at 105599316 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Leucine
at position 570
(V570L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000038665
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046638]
[ENSMUST00000176924]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000046638
AA Change: V570L
PolyPhen 2
Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
|
| SMART Domains |
Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: V570L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Methyltransf_32
|
149 |
413 |
1.9e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176924
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.5%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 46 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcb9 |
CGG |
CG |
5: 124,215,368 (GRCm39) |
|
probably null |
Het |
| Ahcyl1 |
A |
G |
3: 107,577,533 (GRCm39) |
S296P |
probably damaging |
Het |
| Alas1 |
A |
G |
9: 106,113,673 (GRCm39) |
V524A |
probably damaging |
Het |
| Amn |
C |
A |
12: 111,238,246 (GRCm39) |
D53E |
probably damaging |
Het |
| Bbof1 |
A |
G |
12: 84,470,223 (GRCm39) |
E33G |
probably damaging |
Het |
| Ccdc149 |
T |
C |
5: 52,578,114 (GRCm39) |
E106G |
probably damaging |
Het |
| Ceacam5 |
T |
A |
7: 17,479,560 (GRCm39) |
S226T |
probably damaging |
Het |
| Chd7 |
G |
T |
4: 8,801,350 (GRCm39) |
S698I |
possibly damaging |
Het |
| Chst2 |
A |
G |
9: 95,288,148 (GRCm39) |
L66P |
probably damaging |
Het |
| Cps1 |
C |
T |
1: 67,195,383 (GRCm39) |
P268L |
probably damaging |
Het |
| Csmd3 |
CCTTTGCGCTT |
CCTT |
15: 47,604,632 (GRCm39) |
|
probably null |
Het |
| Cyp3a59 |
A |
T |
5: 146,036,622 (GRCm39) |
M275L |
probably benign |
Het |
| Ddx41 |
T |
G |
13: 55,682,113 (GRCm39) |
M232L |
probably benign |
Het |
| Dnah6 |
C |
A |
6: 73,133,404 (GRCm39) |
A1068S |
possibly damaging |
Het |
| Dnah7a |
T |
C |
1: 53,621,661 (GRCm39) |
I1155V |
probably benign |
Het |
| Dop1b |
G |
A |
16: 93,567,565 (GRCm39) |
V611I |
probably damaging |
Het |
| Epyc |
A |
G |
10: 97,512,875 (GRCm39) |
Y243C |
probably damaging |
Het |
| Fam171a1 |
C |
T |
2: 3,226,570 (GRCm39) |
Q568* |
probably null |
Het |
| Gm5930 |
A |
G |
14: 44,573,918 (GRCm39) |
S105P |
probably damaging |
Het |
| Golga4 |
A |
G |
9: 118,389,810 (GRCm39) |
D2032G |
probably benign |
Het |
| Gps2 |
AGTGCT |
A |
11: 69,806,207 (GRCm39) |
|
probably null |
Het |
| H2-DMb1 |
A |
G |
17: 34,376,289 (GRCm39) |
Y136C |
probably damaging |
Het |
| Il12b |
A |
G |
11: 44,301,039 (GRCm39) |
E185G |
probably benign |
Het |
| Kat7 |
A |
C |
11: 95,182,407 (GRCm39) |
I231R |
probably benign |
Het |
| Kcmf1 |
A |
T |
6: 72,827,466 (GRCm39) |
I58N |
probably damaging |
Het |
| Lmf2 |
A |
T |
15: 89,235,966 (GRCm39) |
V646E |
possibly damaging |
Het |
| Lmo7 |
A |
G |
14: 102,126,121 (GRCm39) |
Q409R |
probably damaging |
Het |
| Lmod1 |
T |
A |
1: 135,292,253 (GRCm39) |
H369Q |
probably benign |
Het |
| M1ap |
G |
A |
6: 82,933,484 (GRCm39) |
A13T |
probably benign |
Het |
| Mapk8ip2 |
G |
T |
15: 89,343,168 (GRCm39) |
V637L |
probably benign |
Het |
| Mfsd1 |
T |
A |
3: 67,508,668 (GRCm39) |
N449K |
probably damaging |
Het |
| Or56a5 |
A |
T |
7: 104,793,020 (GRCm39) |
M166K |
probably benign |
Het |
| Or5b24 |
T |
C |
19: 12,912,383 (GRCm39) |
S94P |
possibly damaging |
Het |
| Or5d18 |
T |
A |
2: 87,865,379 (GRCm39) |
I35F |
probably damaging |
Het |
| Or6c209 |
G |
A |
10: 129,483,711 (GRCm39) |
C238Y |
probably benign |
Het |
| Pcdhb5 |
T |
A |
18: 37,455,169 (GRCm39) |
S516R |
probably benign |
Het |
| Plppr3 |
C |
T |
10: 79,701,194 (GRCm39) |
M549I |
possibly damaging |
Het |
| Ppl |
A |
G |
16: 4,912,361 (GRCm39) |
V740A |
probably benign |
Het |
| Rabgef1 |
C |
T |
5: 130,240,928 (GRCm39) |
T349M |
probably benign |
Het |
| Rad51ap2 |
T |
C |
12: 11,507,109 (GRCm39) |
C344R |
probably benign |
Het |
| Shank2 |
A |
G |
7: 143,611,455 (GRCm39) |
Q172R |
possibly damaging |
Het |
| Smg9 |
G |
A |
7: 24,119,546 (GRCm39) |
|
probably null |
Het |
| Tectb |
C |
G |
19: 55,169,431 (GRCm39) |
|
probably benign |
Het |
| Trim42 |
A |
T |
9: 97,241,293 (GRCm39) |
N646K |
probably damaging |
Het |
| Usp32 |
T |
C |
11: 84,896,735 (GRCm39) |
I1181V |
probably benign |
Het |
| Vwa5b1 |
C |
A |
4: 138,319,221 (GRCm39) |
|
probably null |
Het |
|
| Other mutations in Mettl25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00310:Mettl25
|
APN |
10 |
105,662,295 (GRCm39) |
missense |
probably benign |
|
|
IGL00698:Mettl25
|
APN |
10 |
105,629,201 (GRCm39) |
missense |
probably null |
0.31 |
|
IGL00766:Mettl25
|
APN |
10 |
105,615,443 (GRCm39) |
splice site |
probably benign |
|
|
IGL01360:Mettl25
|
APN |
10 |
105,659,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Mettl25
|
APN |
10 |
105,659,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02088:Mettl25
|
APN |
10 |
105,659,111 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02623:Mettl25
|
APN |
10 |
105,662,185 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03245:Mettl25
|
APN |
10 |
105,662,358 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL03134:Mettl25
|
UTSW |
10 |
105,661,888 (GRCm39) |
nonsense |
probably null |
|
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1297:Mettl25
|
UTSW |
10 |
105,659,126 (GRCm39) |
missense |
probably benign |
0.11 |
|
R1482:Mettl25
|
UTSW |
10 |
105,662,451 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1526:Mettl25
|
UTSW |
10 |
105,668,844 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R1542:Mettl25
|
UTSW |
10 |
105,661,981 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1589:Mettl25
|
UTSW |
10 |
105,615,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1901:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1902:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R2016:Mettl25
|
UTSW |
10 |
105,633,167 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2920:Mettl25
|
UTSW |
10 |
105,601,038 (GRCm39) |
splice site |
probably null |
|
|
R3149:Mettl25
|
UTSW |
10 |
105,662,214 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4547:Mettl25
|
UTSW |
10 |
105,661,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4646:Mettl25
|
UTSW |
10 |
105,662,416 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4652:Mettl25
|
UTSW |
10 |
105,615,509 (GRCm39) |
nonsense |
probably null |
|
|
R5020:Mettl25
|
UTSW |
10 |
105,662,068 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5435:Mettl25
|
UTSW |
10 |
105,615,447 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6947:Mettl25
|
UTSW |
10 |
105,662,053 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7075:Mettl25
|
UTSW |
10 |
105,665,785 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7729:Mettl25
|
UTSW |
10 |
105,601,871 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8074:Mettl25
|
UTSW |
10 |
105,661,941 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8108:Mettl25
|
UTSW |
10 |
105,659,040 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8415:Mettl25
|
UTSW |
10 |
105,662,002 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8937:Mettl25
|
UTSW |
10 |
105,601,122 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8949:Mettl25
|
UTSW |
10 |
105,668,714 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9497:Mettl25
|
UTSW |
10 |
105,677,090 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9772:Mettl25
|
UTSW |
10 |
105,633,127 (GRCm39) |
missense |
probably benign |
|
|
RF009:Mettl25
|
UTSW |
10 |
105,669,100 (GRCm39) |
intron |
probably benign |
|
|
Z1176:Mettl25
|
UTSW |
10 |
105,661,959 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- CTGTATGCTTTGAGAATGAAAACAC -3'
(R):5'- ACTGTGGCAGCCAATCTCTATAG -3'
Sequencing Primer
(F):5'- TCTAGCACTCTGCTTCAG -3'
(R):5'- CTATACTGGGGCCTAGAAACTTC -3'
|
| Posted On |
2014-10-30 |
Incidental Mutation