|Institutional Source||Beutler Lab|
|Gene Name||WEE 1 homolog 1 (S. pombe)|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R3110 (G1)|
|Chromosomal Location||110122046-110143286 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 110130836 bp|
|Amino Acid Change||Serine to Arginine at position 382 (S382R)|
|Ref Sequence||ENSEMBL: ENSMUSP00000033326 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033326]|
|Predicted Effect||probably damaging
AA Change: S382R
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: S382R
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.8916|
|Coding Region Coverage||
|Validation Efficiency||98% (51/52)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Wee1||
(F):5'- TGAACAAAAGTTGGTTGCTGAG -3'
(R):5'- AAGACTTGGGCCAGTGTCAG -3'
(F):5'- GTCTGGGCTAGCTAACCTTAAAC -3'
(R):5'- CCAGTGTCAGAGTGGTCTAATC -3'