Incidental Mutation 'R4051:Ier5l'
ID314144
Institutional Source Beutler Lab
Gene Symbol Ier5l
Ensembl Gene ENSMUSG00000089762
Gene Nameimmediate early response 5-like
Synonyms
Accession Numbers
Is this an essential gene? Not available question?
Stock #R4051 ()
Quality Score225
Status Not validated
Chromosome2
Chromosomal Location30471537-30474219 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to C at 30473312 bp
ZygosityHeterozygous
Amino Acid Change Serine to Alanine at position 234 (S234A)
Ref Sequence ENSEMBL: ENSMUSP00000132594 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000065134]
Predicted Effect probably benign
Transcript: ENSMUST00000065134
AA Change: S234A

PolyPhen 2 Score 0.317 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000132594
Gene: ENSMUSG00000089762
AA Change: S234A

DomainStartEndE-ValueType
Pfam:IER 1 406 8.1e-141 PFAM
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 93.9%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 30 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcc6 A C 7: 45,986,563 L1020R probably damaging Het
Ahnak A C 19: 9,014,327 D4325A probably damaging Het
Chtf18 A G 17: 25,719,194 V955A probably damaging Het
Dclk2 A G 3: 86,830,822 probably null Het
Disc1 A G 8: 125,148,425 T547A possibly damaging Het
Eif4b A G 15: 102,086,604 Y211C probably benign Het
Fli1 T G 9: 32,452,162 D176A probably benign Het
Hcn2 A C 10: 79,733,687 probably null Het
Ice1 A G 13: 70,603,527 V1480A probably damaging Het
Ino80b G C 6: 83,122,333 P178R probably damaging Het
Itga11 C T 9: 62,755,651 Q550* probably null Het
Kdr T A 5: 75,968,408 M193L probably benign Het
Kif3b G A 2: 153,323,557 R628Q probably damaging Het
Ldlrad3 C T 2: 101,953,162 D240N probably damaging Het
Met T C 6: 17,548,729 V924A possibly damaging Het
Olfr554 T C 7: 102,641,026 V260A possibly damaging Het
Peg10 T C 6: 4,754,534 L105P probably benign Het
Phtf1 A G 3: 104,005,508 T717A possibly damaging Het
Ptafr T C 4: 132,579,994 W232R probably benign Het
Rnase4 A G 14: 51,105,005 K62R probably benign Het
Slamf7 T C 1: 171,637,383 K261E possibly damaging Het
Slc9c1 T A 16: 45,543,230 Y120N probably damaging Het
Sstr2 A C 11: 113,624,656 T134P probably damaging Het
Steap4 A G 5: 7,980,404 Y420C probably damaging Het
Tbc1d9b T C 11: 50,171,243 C1210R probably benign Het
Tnfsf10 T A 3: 27,335,354 I188N probably damaging Het
Trav7-3 A G 14: 53,443,746 T82A probably benign Het
Usp46 T C 5: 74,002,755 N283S probably benign Het
Vmn2r84 T G 10: 130,390,898 N357T probably damaging Het
Zfp217 T C 2: 170,112,616 probably null Het
Other mutations in Ier5l
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01610:Ier5l APN 2 30473954 missense probably damaging 1.00
IGL03209:Ier5l APN 2 30473055 missense possibly damaging 0.84
R2073:Ier5l UTSW 2 30473056 missense probably damaging 0.99
R5727:Ier5l UTSW 2 30473159 missense possibly damaging 0.86
RF035:Ier5l UTSW 2 30473820 small deletion probably benign
X0021:Ier5l UTSW 2 30473820 small deletion probably benign
Predicted Primers PCR Primer
(F):5'- GGGTAATACTTGCGCTTGC -3'
(R):5'- TCTACCTGAGCGAGCGTTAC -3'

Sequencing Primer
(F):5'- GACGCCAGACCGGGTCC -3'
(R):5'- TGCACCTCCAGCAGCAG -3'
Posted On2015-04-30