Mutagenetix > Incidental Mutations

Incidental Mutation 'R4066:Togaram2'

316134

Institutional Source

Beutler Lab

Gene Symbol

Togaram2

Ensembl Gene

ENSMUSG00000045761

Gene Name

TOG array regulator of axonemal microtubules 2

Synonyms

Fam179a, 4632412N22Rik

MMRRC Submission

040973-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R4066 (G1)

Quality Score

186

Status

Validated

Chromosome

Chromosomal Location

71673261-71729669 bp(+) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

C to A at 71716238 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000122691 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000097284] [ENSMUST00000144479] [ENSMUST00000153445]

Predicted Effect

probably benign
Transcript: ENSMUST00000097284

SMART Domains

Protein: ENSMUSP00000094886
Gene: ENSMUSG00000045761

Domain	Start	End	E-Value	Type
low complexity region	49	60	N/A	INTRINSIC
low complexity region	94	105	N/A	INTRINSIC
low complexity region	467	474	N/A	INTRINSIC
Pfam:CLASP_N	492	705	2.3e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129072

Predicted Effect

probably benign
Transcript: ENSMUST00000144479

SMART Domains

Protein: ENSMUSP00000114359
Gene: ENSMUSG00000045761

Domain	Start	End	E-Value	Type
low complexity region	50	61	N/A	INTRINSIC
low complexity region	95	106	N/A	INTRINSIC
low complexity region	468	475	N/A	INTRINSIC
Pfam:CLASP_N	493	706	2.4e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000153445

SMART Domains

Protein: ENSMUSP00000122691
Gene: ENSMUSG00000045761

Domain	Start	End	E-Value	Type
low complexity region	49	60	N/A	INTRINSIC
low complexity region	94	105	N/A	INTRINSIC
low complexity region	467	474	N/A	INTRINSIC
Pfam:CLASP_N	492	705	2.3e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161407

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

96% (50/52)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam23	A	T	1: 63,563,425	H582L	probably damaging	Het
Ankrd13d	C	T	19: 4,270,360	A118T	probably benign	Het
Arhgap30	C	T	1: 171,408,323	T755I	probably benign	Het
Cab39l	A	G	14: 59,547,005	H285R	probably benign	Het
Dpyd	AAT	AATGTATATATAT	3: 118,897,089		probably benign	Het
Dspp	T	A	5: 104,177,194	N474K	unknown	Het
Fanci	T	C	7: 79,412,757		probably null	Het
Fras1	T	A	5: 96,770,683	I3526K	possibly damaging	Het
Fut8	T	A	12: 77,464,061	Y421N	probably damaging	Het
Gm12258	C	T	11: 58,858,526	L176F	probably benign	Het
Gm8220	A	T	14: 44,285,638	R12*	probably null	Het
Gm9825	T	A	6: 7,983,009		noncoding transcript	Het
Hecw1	T	C	13: 14,316,431	S659G	probably damaging	Het
Hnrnpr	C	A	4: 136,339,346		probably benign	Het
Htt	C	T	5: 34,878,847	T2046I	probably benign	Het
Kat7	T	C	11: 95,284,141	D259G	possibly damaging	Het
Klra9	T	C	6: 130,188,744	T103A	probably benign	Het
Lad1	A	G	1: 135,827,427	E147G	probably damaging	Het
Lipo2	T	C	19: 33,720,859	I373V	probably benign	Het
Ltb4r1	G	T	14: 55,767,495	W85L	probably damaging	Het
Ltn1	T	C	16: 87,416,230	Y481C	possibly damaging	Het
Man1c1	G	C	4: 134,703,438	P11R	probably damaging	Het
Muc4	A	T	16: 32,751,051	I310F	possibly damaging	Het
Myl10	A	G	5: 136,695,450	K70E	probably damaging	Het
Nptx2	G	T	5: 144,556,312	W403L	probably damaging	Het
Nyap2	A	G	1: 81,241,835	Y524C	probably damaging	Het
Olfr1245	T	A	2: 89,575,179	L182F	probably damaging	Het
Olfr1415	A	G	1: 92,491,189	C189R	probably damaging	Het
Olfr906	T	A	9: 38,488,482	M151K	probably benign	Het
Pde9a	A	G	17: 31,443,838	*64W	probably null	Het
Ppp2r5b	T	C	19: 6,229,330	Y379C	probably damaging	Het
Rd3l	T	G	12: 111,979,511	N178T	probably benign	Het
Recql4	A	G	15: 76,705,827	Y673H	probably damaging	Het
Shcbp1	A	G	8: 4,748,716	I401T	probably damaging	Het
Shd	A	T	17: 55,971,581	D48V	probably damaging	Het
Slc14a1	A	G	18: 78,111,377	W209R	probably damaging	Het
Slc2a9	T	C	5: 38,483,349	K6E	probably benign	Het
Slco6d1	A	G	1: 98,463,846		probably benign	Het
Spic	T	C	10: 88,675,683	H237R	possibly damaging	Het
Stau2	A	G	1: 16,394,059	S156P	possibly damaging	Het
Stmn2	T	C	3: 8,509,608		probably benign	Het
Trpc5	T	A	X: 144,419,598	R545*	probably null	Het
Ugcg	C	T	4: 59,207,798	P46S	probably benign	Het
Wdfy3	C	T	5: 101,922,447	V1152I	probably benign	Het
Xpo4	G	T	14: 57,588,054	H939N	probably benign	Het

Other mutations in Togaram2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00943:Togaram2	APN	17	71725004	missense	probably damaging	1.00
IGL01298:Togaram2	APN	17	71716513	missense	possibly damaging	0.71
IGL01625:Togaram2	APN	17	71714698	missense	probably benign	0.06
IGL01691:Togaram2	APN	17	71729490	missense	probably null	0.02
IGL02165:Togaram2	APN	17	71697866	missense	probably benign	0.00
IGL02186:Togaram2	APN	17	71685171	missense	possibly damaging	0.64
IGL02664:Togaram2	APN	17	71729239	missense	probably damaging	0.97
IGL02712:Togaram2	APN	17	71704754	missense	probably benign	0.04
IGL03000:Togaram2	APN	17	71717370	missense	probably benign	0.08
IGL03209:Togaram2	APN	17	71695745	critical splice donor site	probably null
R0211:Togaram2	UTSW	17	71729248	missense	probably damaging	1.00
R0212:Togaram2	UTSW	17	71724983	missense	probably damaging	1.00
R0219:Togaram2	UTSW	17	71714230	splice site	probably benign
R0268:Togaram2	UTSW	17	71697998	critical splice donor site	probably null
R0617:Togaram2	UTSW	17	71700509	missense	possibly damaging	0.87
R0831:Togaram2	UTSW	17	71716444	missense	probably damaging	1.00
R0972:Togaram2	UTSW	17	71707314	missense	probably damaging	1.00
R1635:Togaram2	UTSW	17	71697851	missense	probably benign	0.05
R1799:Togaram2	UTSW	17	71691455	missense	probably damaging	1.00
R2062:Togaram2	UTSW	17	71716365	missense	probably benign	0.26
R2414:Togaram2	UTSW	17	71716309	intron	probably benign
R2866:Togaram2	UTSW	17	71709597	missense	probably benign	0.00
R2867:Togaram2	UTSW	17	71709597	missense	probably benign	0.00
R2867:Togaram2	UTSW	17	71709597	missense	probably benign	0.00
R4807:Togaram2	UTSW	17	71697923	missense	probably damaging	1.00
R5659:Togaram2	UTSW	17	71687672	missense	probably damaging	0.96
R5680:Togaram2	UTSW	17	71689209	missense	probably benign	0.00
R5975:Togaram2	UTSW	17	71729205	missense	probably damaging	1.00
R5996:Togaram2	UTSW	17	71704783	missense	probably damaging	0.99
R6619:Togaram2	UTSW	17	71689271	missense	probably damaging	0.99
R6682:Togaram2	UTSW	17	71704754	missense	probably benign	0.04
R6922:Togaram2	UTSW	17	71707134	missense	probably damaging	1.00
R6956:Togaram2	UTSW	17	71729188	missense	probably benign	0.00
R6968:Togaram2	UTSW	17	71709613	missense	probably damaging	1.00
R7007:Togaram2	UTSW	17	71709643	missense	probably damaging	0.99
R7015:Togaram2	UTSW	17	71709568	missense	possibly damaging	0.62
R7140:Togaram2	UTSW	17	71714766	missense	probably benign	0.00
R7383:Togaram2	UTSW	17	71700517	missense	probably damaging	1.00
R7691:Togaram2	UTSW	17	71716410	missense	probably benign	0.16
R7778:Togaram2	UTSW	17	71704751	missense	probably benign	0.00
R7824:Togaram2	UTSW	17	71704751	missense	probably benign	0.00
R7862:Togaram2	UTSW	17	71689173	missense	probably benign	0.00
R7864:Togaram2	UTSW	17	71700940	missense	probably damaging	0.96
R7945:Togaram2	UTSW	17	71689173	missense	probably benign	0.00
R7947:Togaram2	UTSW	17	71700940	missense	probably damaging	0.96
X0063:Togaram2	UTSW	17	71707197	missense	possibly damaging	0.91
Z1088:Togaram2	UTSW	17	71714280	missense	possibly damaging	0.87
Z1177:Togaram2	UTSW	17	71701002	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TCTTTACACATCCTCAGAAAAGGG -3'
(R):5'- TACTCAAGGAGCTTCCCAACG -3'

Sequencing Primer
(F):5'- TTACACATCCTCAGAAAAGGGAAATG -3'
(R):5'- ATCCGAGCCTGGAACTCCTTG -3'

Posted On

2015-05-15