Mutagenetix > Incidental Mutation

Incidental Mutation 'R4649:Myt1'

350561

Institutional Source

Beutler Lab

Gene Symbol

Myt1

Ensembl Gene

ENSMUSG00000010505

Gene Name

myelin transcription factor 1

Synonyms

NZF-2b, NZF-2a, Nztf2, Nzf2

MMRRC Submission

041910-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4649 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

181405125-181469590 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 181439207 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 243 (D243G)

Ref Sequence

ENSEMBL: ENSMUSP00000104388 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081125] [ENSMUST00000108756] [ENSMUST00000108757] [ENSMUST00000183403]

AlphaFold

Q8CFC2

Predicted Effect

probably benign
Transcript: ENSMUST00000081125
AA Change: D285G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000079900
Gene: ENSMUSG00000010505
AA Change: D285G

Domain	Start	End	E-Value	Type
Pfam:zf-C2HC	71	99	8.7e-16	PFAM
low complexity region	155	160	N/A	INTRINSIC
low complexity region	185	201	N/A	INTRINSIC
coiled coil region	300	354	N/A	INTRINSIC
low complexity region	388	405	N/A	INTRINSIC
Pfam:zf-C2HC	485	512	2.9e-14	PFAM
Pfam:zf-C2HC	529	557	4.3e-16	PFAM
Pfam:MYT1	604	660	2e-28	PFAM
Pfam:MYT1	659	835	2.3e-56	PFAM
Pfam:zf-C2HC	843	871	2e-18	PFAM
Pfam:zf-C2HC	887	915	1.9e-18	PFAM
Pfam:zf-C2HC	936	964	2.1e-16	PFAM
Pfam:zf-C2HC	989	1017	8.4e-16	PFAM
coiled coil region	1037	1109	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108756
AA Change: D243G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000104387
Gene: ENSMUSG00000010505
AA Change: D243G

Domain	Start	End	E-Value	Type
Pfam:zf-C2HC	27	57	5.1e-18	PFAM
low complexity region	113	118	N/A	INTRINSIC
low complexity region	143	159	N/A	INTRINSIC
coiled coil region	258	312	N/A	INTRINSIC
low complexity region	346	363	N/A	INTRINSIC
Pfam:zf-C2HC	441	471	5e-17	PFAM
Pfam:zf-C2HC	485	515	3.1e-18	PFAM
Pfam:MYT1	562	618	2.4e-32	PFAM
Pfam:MYT1	617	794	2e-74	PFAM
Pfam:zf-C2HC	799	829	1.9e-19	PFAM
Pfam:zf-C2HC	843	873	9.7e-20	PFAM
Pfam:zf-C2HC	892	922	2.2e-18	PFAM
Pfam:zf-C2HC	945	975	1.7e-16	PFAM
coiled coil region	995	1067	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108757
AA Change: D243G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000104388
Gene: ENSMUSG00000010505
AA Change: D243G

Domain	Start	End	E-Value	Type
Pfam:zf-C2HC	27	57	1e-17	PFAM
low complexity region	113	118	N/A	INTRINSIC
low complexity region	143	159	N/A	INTRINSIC
coiled coil region	258	312	N/A	INTRINSIC
low complexity region	346	363	N/A	INTRINSIC
Pfam:zf-C2HC	441	471	1e-16	PFAM
Pfam:zf-C2HC	485	510	6.2e-12	PFAM
Pfam:MYT1	524	580	2.7e-32	PFAM
Pfam:MYT1	579	756	2.3e-74	PFAM
Pfam:zf-C2HC	761	791	3.8e-19	PFAM
Pfam:zf-C2HC	805	835	1.9e-19	PFAM
Pfam:zf-C2HC	854	884	4.3e-18	PFAM
Pfam:zf-C2HC	907	937	3.3e-16	PFAM
coiled coil region	957	1029	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000129843

SMART Domains

Protein: ENSMUSP00000115697
Gene: ENSMUSG00000010505

Domain	Start	End	E-Value	Type
low complexity region	50	67	N/A	INTRINSIC
Pfam:zf-C2HC	147	174	6.2e-15	PFAM
Pfam:zf-C2HC	191	219	9.2e-17	PFAM
Pfam:MYT1	266	322	7.3e-29	PFAM
Pfam:MYT1	321	497	7.2e-57	PFAM
Pfam:zf-C2HC	505	533	9.6e-19	PFAM
Pfam:zf-C2HC	554	582	4.4e-17	PFAM
Pfam:zf-C2HC	607	635	1.8e-16	PFAM
coiled coil region	654	726	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000129856

SMART Domains

Protein: ENSMUSP00000122011
Gene: ENSMUSG00000010505

Domain	Start	End	E-Value	Type
Pfam:zf-C2HC	63	91	1.4e-16	PFAM
low complexity region	147	152	N/A	INTRINSIC
Pfam:zf-C2HC	177	204	4.6e-15	PFAM
Pfam:zf-C2HC	221	249	6.7e-17	PFAM
Pfam:MYT1	296	352	5.2e-29	PFAM
Pfam:MYT1	351	527	4.5e-57	PFAM
Pfam:zf-C2HC	535	556	1.4e-13	PFAM
Pfam:zf-C2HC	556	584	3.5e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130079

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142245

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156190

Predicted Effect

probably benign
Transcript: ENSMUST00000183403

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene is a member of the myelin transcription factor 1 gene family. The encoded protein, a zinc finger DNA-binding protein, is involved in regulation of oligodendrocyte differentiation and proliferation in the developing central nervous system. The gene product has a role in remyelination through regeneration of oligodendrocyte lineage cells in response to demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele die shortly after birth displaying improper diaphragm innervation and a failture to initiate breathing; mutant embryonic pancreata contain abnormal islet cells that express multiple hormones. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
4933409G03Rik	G	A	2: 68,436,559 (GRCm39)	E168K	unknown	Het
Abca4	G	T	3: 121,963,542 (GRCm39)	V841F	probably damaging	Het
Adcy10	G	A	1: 165,331,618 (GRCm39)	E84K	probably damaging	Het
Adgrg6	C	A	10: 14,344,571 (GRCm39)	M127I	probably damaging	Het
Agpat5	A	G	8: 18,929,668 (GRCm39)	I266V	possibly damaging	Het
Agtpbp1	A	T	13: 59,676,213 (GRCm39)	Y186N	possibly damaging	Het
Alx1	A	T	10: 102,845,193 (GRCm39)	N279K	probably damaging	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Arsi	A	G	18: 61,050,170 (GRCm39)	Y351C	probably damaging	Het
B4galnt3	T	A	6: 120,201,581 (GRCm39)	R155W	probably damaging	Het
BC028528	T	C	3: 95,795,577 (GRCm39)	E75G	probably damaging	Het
Bmp2k	C	T	5: 97,200,970 (GRCm39)	P297S	possibly damaging	Het
Btnl4	A	G	17: 34,691,602 (GRCm39)	S296P	probably benign	Het
Bzw2	A	T	12: 36,173,980 (GRCm39)	V98E	probably null	Het
Cacna1d	C	T	14: 29,817,365 (GRCm39)	M1232I	probably benign	Het
Cast	A	G	13: 74,894,133 (GRCm39)	S171P	probably benign	Het
Catsperb	G	A	12: 101,507,771 (GRCm39)	A513T	probably benign	Het
Cd19	A	G	7: 126,013,664 (GRCm39)	S42P	probably benign	Het
Chml	A	G	1: 175,514,962 (GRCm39)	S138P	probably benign	Het
Clic6	T	G	16: 92,327,827 (GRCm39)		probably null	Het
Col12a1	A	T	9: 79,547,076 (GRCm39)	V2252E	probably damaging	Het
Cplx2	G	A	13: 54,527,361 (GRCm39)	E82K	probably benign	Het
Csmd2	G	A	4: 128,439,866 (GRCm39)	V3107M	probably benign	Het
Cspg4	T	A	9: 56,794,149 (GRCm39)	V628D	possibly damaging	Het
Dcaf13	G	A	15: 39,001,637 (GRCm39)	R315Q	possibly damaging	Het
Deaf1	A	G	7: 140,877,486 (GRCm39)	V547A	possibly damaging	Het
Dmxl1	C	T	18: 50,011,698 (GRCm39)	T1285I	probably damaging	Het
Dnah3	C	A	7: 119,646,921 (GRCm39)	W1036L	probably damaging	Het
Dnai3	A	G	3: 145,753,922 (GRCm39)	S713P	probably damaging	Het
Dpy19l1	A	C	9: 24,393,350 (GRCm39)	S140A	possibly damaging	Het
Dsg2	T	A	18: 20,735,302 (GRCm39)	D1093E	possibly damaging	Het
Edaradd	T	A	13: 12,523,304 (GRCm39)	H12L	probably damaging	Het
Enam	T	C	5: 88,640,827 (GRCm39)	M163T	probably benign	Het
Fbxo22	T	A	9: 55,128,333 (GRCm39)	V218E	probably damaging	Het
Fbxo34	A	T	14: 47,767,085 (GRCm39)	K148N	probably damaging	Het
Foxi3	A	G	6: 70,933,960 (GRCm39)	D149G	probably damaging	Het
Gbp8	C	T	5: 105,189,807 (GRCm39)	G75D	probably damaging	Het
Gm10784	C	T	13: 50,099,251 (GRCm39)		noncoding transcript	Het
Gm7247	G	A	14: 51,807,051 (GRCm39)		probably null	Het
Grik3	T	A	4: 125,544,278 (GRCm39)	V333E	probably damaging	Het
Gsap	A	T	5: 21,431,309 (GRCm39)	D211V	probably damaging	Het
H2-T15	T	C	17: 36,368,768 (GRCm39)	Y104C	possibly damaging	Het
Ino80	T	C	2: 119,261,489 (GRCm39)	N725S	probably damaging	Het
Itgad	A	G	7: 127,788,703 (GRCm39)	I443V	probably benign	Het
Itsn1	T	A	16: 91,638,476 (GRCm39)	I52N	probably damaging	Het
Kmt2b	TCTCCTCCTCCTCCTCCTCCTCCTCC	TCTCCTCCTCCTCCTCCTCCTCC	7: 30,285,783 (GRCm39)		probably benign	Het
Lamc1	T	G	1: 153,104,523 (GRCm39)	S59R	probably damaging	Het
Lrrk1	T	C	7: 65,922,801 (GRCm39)	I1366V	probably benign	Het
Lyn	C	A	4: 3,738,850 (GRCm39)	Q41K	probably benign	Het
Mapk13	C	T	17: 28,997,461 (GRCm39)	R360*	probably null	Het
Mapk8ip3	T	A	17: 25,123,726 (GRCm39)	I599F	probably damaging	Het
Me1	T	C	9: 86,561,905 (GRCm39)	K60R	probably benign	Het
Mga	T	A	2: 119,771,974 (GRCm39)	D1532E	possibly damaging	Het
Miga1	G	A	3: 151,984,642 (GRCm39)	S427L	probably benign	Het
Mknk2	A	T	10: 80,505,173 (GRCm39)	V186E	probably damaging	Het
Myo1a	C	A	10: 127,546,086 (GRCm39)	N236K	probably benign	Het
Ncf4	T	C	15: 78,140,189 (GRCm39)	V196A	possibly damaging	Het
Nlrp5	G	T	7: 23,117,603 (GRCm39)	L442F	probably damaging	Het
Olfm4	G	A	14: 80,258,747 (GRCm39)	D332N	probably benign	Het
Or4a81	C	A	2: 89,619,637 (GRCm39)	V20F	probably benign	Het
Or4b1d	T	A	2: 89,969,432 (GRCm39)	D17V	probably damaging	Het
Osbpl11	T	A	16: 33,016,452 (GRCm39)	M69K	probably benign	Het
Oxnad1	T	A	14: 31,824,366 (GRCm39)	*312K	probably null	Het
Plekhg1	A	G	10: 3,906,985 (GRCm39)	Q634R	probably benign	Het
Plekhh2	T	C	17: 84,882,691 (GRCm39)	Y733H	probably damaging	Het
Ppp1r21	A	G	17: 88,856,941 (GRCm39)	H132R	probably benign	Het
Prkcq	T	C	2: 11,284,333 (GRCm39)	V501A	possibly damaging	Het
Prpf38b	G	A	3: 108,811,408 (GRCm39)		probably benign	Het
Prpf4b	A	T	13: 35,083,954 (GRCm39)	M908L	probably benign	Het
Prps2	T	C	X: 166,135,288 (GRCm39)	D183G	probably damaging	Het
Pudp	T	C	18: 50,701,259 (GRCm39)	D158G	probably damaging	Het
R3hdm1	T	C	1: 128,112,181 (GRCm39)	S422P	probably damaging	Het
Ranbp3	T	A	17: 57,003,640 (GRCm39)		probably null	Het
Ranbp6	A	G	19: 29,787,721 (GRCm39)	V877A	probably benign	Het
Rgsl1	A	T	1: 153,693,328 (GRCm39)	S618T	probably benign	Het
Rhox2a	A	T	X: 36,509,015 (GRCm39)	I61L	probably benign	Het
Rhox2a	G	C	X: 36,508,962 (GRCm39)	R43P	probably benign	Het
Rnf207	A	C	4: 152,396,612 (GRCm39)	D466E	probably benign	Het
Rnf7	A	G	9: 96,353,883 (GRCm39)	I80T	probably benign	Het
Ros1	T	A	10: 52,005,764 (GRCm39)	I878F	possibly damaging	Het
Rsbn1	T	A	3: 103,861,096 (GRCm39)		probably null	Het
Sbk1	T	C	7: 125,890,301 (GRCm39)	S105P	probably damaging	Het
Sdk1	T	C	5: 141,992,380 (GRCm39)	Y790H	probably damaging	Het
Sec62	T	A	3: 30,864,683 (GRCm39)	N182K	unknown	Het
Slc15a1	T	C	14: 121,715,504 (GRCm39)	Y345C	probably damaging	Het
Slc27a1	G	A	8: 72,023,408 (GRCm39)	A15T	probably benign	Het
Slc35d1	A	G	4: 103,070,426 (GRCm39)	F110L	probably damaging	Het
Slc39a5	T	A	10: 128,233,136 (GRCm39)	S333C	probably benign	Het
Tarbp1	A	G	8: 127,173,934 (GRCm39)	S927P	probably damaging	Het
Tns1	A	G	1: 73,992,930 (GRCm39)	S583P	probably damaging	Het
Trim38	A	T	13: 23,966,952 (GRCm39)	D133V	probably damaging	Het
Trim40	T	C	17: 37,193,531 (GRCm39)		probably null	Het
Trim42	C	A	9: 97,244,998 (GRCm39)	V601L	probably benign	Het
Trpc7	G	A	13: 57,035,367 (GRCm39)	R189C	probably damaging	Het
Ttc29	A	T	8: 79,060,208 (GRCm39)	Y376F	probably benign	Het
Vmn2r63	A	G	7: 42,553,114 (GRCm39)	I714T	possibly damaging	Het
Vmn2r68	C	A	7: 84,870,743 (GRCm39)	A847S	probably benign	Het
Zbp1	G	A	2: 173,049,608 (GRCm39)	P385S	possibly damaging	Het
Zfat	A	G	15: 68,056,325 (GRCm39)	Y237H	probably damaging	Het
Zfp236	A	T	18: 82,615,784 (GRCm39)	W1840R	probably damaging	Het
Zfp426	G	T	9: 20,381,923 (GRCm39)	Q341K	possibly damaging	Het
Zfp462	T	A	4: 55,009,349 (GRCm39)	N438K	probably benign	Het
Zfp952	T	A	17: 33,221,899 (GRCm39)	V88E	probably damaging	Het
Znfx1	G	T	2: 166,898,276 (GRCm39)	A216D	probably benign	Het

Other mutations in Myt1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Myt1	APN	2	181,442,908 (GRCm39)	missense	probably damaging	1.00
IGL00816:Myt1	APN	2	181,449,308 (GRCm39)	missense	probably damaging	0.97
IGL01062:Myt1	APN	2	181,439,522 (GRCm39)	missense	probably damaging	1.00
IGL01069:Myt1	APN	2	181,467,749 (GRCm39)	missense	probably damaging	1.00
IGL01292:Myt1	APN	2	181,446,805 (GRCm39)	missense	probably damaging	1.00
IGL01521:Myt1	APN	2	181,467,704 (GRCm39)	missense	probably damaging	1.00
IGL01926:Myt1	APN	2	181,463,790 (GRCm39)	missense	probably benign	0.00
IGL01976:Myt1	APN	2	181,437,532 (GRCm39)	missense	probably damaging	1.00
IGL02066:Myt1	APN	2	181,438,982 (GRCm39)	missense	probably damaging	1.00
IGL02109:Myt1	APN	2	181,457,410 (GRCm39)	splice site	probably benign
IGL02209:Myt1	APN	2	181,439,027 (GRCm39)	missense	probably benign	0.06
IGL02499:Myt1	APN	2	181,467,342 (GRCm39)	splice site	probably benign
IGL03064:Myt1	APN	2	181,439,594 (GRCm39)	missense	probably benign	0.31
IGL03394:Myt1	APN	2	181,439,638 (GRCm39)	missense	probably damaging	1.00
PIT4366001:Myt1	UTSW	2	181,467,731 (GRCm39)	missense	probably damaging	1.00
R0003:Myt1	UTSW	2	181,443,664 (GRCm39)	missense	probably damaging	1.00
R0003:Myt1	UTSW	2	181,443,664 (GRCm39)	missense	probably damaging	1.00
R0362:Myt1	UTSW	2	181,405,186 (GRCm39)	unclassified	probably benign
R0627:Myt1	UTSW	2	181,437,482 (GRCm39)	missense	probably benign	0.10
R0650:Myt1	UTSW	2	181,424,408 (GRCm39)	nonsense	probably null
R0735:Myt1	UTSW	2	181,449,180 (GRCm39)	unclassified	probably benign
R0744:Myt1	UTSW	2	181,439,298 (GRCm39)	intron	probably benign
R1115:Myt1	UTSW	2	181,453,024 (GRCm39)	nonsense	probably null
R1460:Myt1	UTSW	2	181,444,725 (GRCm39)	missense	probably damaging	1.00
R1471:Myt1	UTSW	2	181,438,904 (GRCm39)	missense	probably benign
R1836:Myt1	UTSW	2	181,439,068 (GRCm39)	missense	probably benign
R1905:Myt1	UTSW	2	181,439,549 (GRCm39)	missense	probably damaging	1.00
R2007:Myt1	UTSW	2	181,437,552 (GRCm39)	missense	probably benign
R2040:Myt1	UTSW	2	181,467,717 (GRCm39)	missense	probably damaging	1.00
R2140:Myt1	UTSW	2	181,467,772 (GRCm39)	missense	probably damaging	1.00
R2323:Myt1	UTSW	2	181,448,350 (GRCm39)	missense	probably damaging	1.00
R2926:Myt1	UTSW	2	181,467,803 (GRCm39)	missense	possibly damaging	0.93
R3895:Myt1	UTSW	2	181,461,863 (GRCm39)	missense	probably damaging	1.00
R4093:Myt1	UTSW	2	181,453,191 (GRCm39)	missense	probably damaging	1.00
R4693:Myt1	UTSW	2	181,437,532 (GRCm39)	missense	probably damaging	1.00
R4775:Myt1	UTSW	2	181,464,470 (GRCm39)	missense	probably damaging	1.00
R4835:Myt1	UTSW	2	181,439,255 (GRCm39)	missense	probably damaging	0.99
R5111:Myt1	UTSW	2	181,437,678 (GRCm39)	missense	probably benign	0.01
R5120:Myt1	UTSW	2	181,439,413 (GRCm39)	missense	probably benign	0.25
R5622:Myt1	UTSW	2	181,438,915 (GRCm39)	missense	probably benign
R6457:Myt1	UTSW	2	181,405,218 (GRCm39)	splice site	probably null
R6704:Myt1	UTSW	2	181,453,005 (GRCm39)	start codon destroyed	probably null
R6752:Myt1	UTSW	2	181,442,875 (GRCm39)	missense	probably damaging	1.00
R6944:Myt1	UTSW	2	181,439,387 (GRCm39)	missense	possibly damaging	0.52
R7337:Myt1	UTSW	2	181,444,756 (GRCm39)	missense	possibly damaging	0.71
R7362:Myt1	UTSW	2	181,439,033 (GRCm39)	missense	probably benign	0.00
R7368:Myt1	UTSW	2	181,424,384 (GRCm39)	missense	possibly damaging	0.53
R7385:Myt1	UTSW	2	181,409,498 (GRCm39)	splice site	probably null
R7411:Myt1	UTSW	2	181,456,899 (GRCm39)	missense	probably damaging	1.00
R7593:Myt1	UTSW	2	181,439,532 (GRCm39)	missense	possibly damaging	0.54
R7790:Myt1	UTSW	2	181,439,390 (GRCm39)	missense	probably benign	0.00
R8035:Myt1	UTSW	2	181,437,532 (GRCm39)	missense	probably damaging	1.00
R8156:Myt1	UTSW	2	181,464,554 (GRCm39)	critical splice donor site	probably null
R8338:Myt1	UTSW	2	181,443,655 (GRCm39)	missense	possibly damaging	0.94
R8419:Myt1	UTSW	2	181,424,399 (GRCm39)	nonsense	probably null
R8553:Myt1	UTSW	2	181,439,344 (GRCm39)	missense	possibly damaging	0.91
R9071:Myt1	UTSW	2	181,448,420 (GRCm39)	missense	possibly damaging	0.87
R9144:Myt1	UTSW	2	181,467,805 (GRCm39)	missense	possibly damaging	0.95
R9290:Myt1	UTSW	2	181,437,667 (GRCm39)	missense	probably benign	0.31
R9462:Myt1	UTSW	2	181,467,729 (GRCm39)	nonsense	probably null
R9502:Myt1	UTSW	2	181,461,991 (GRCm39)	missense	probably damaging	0.98
R9668:Myt1	UTSW	2	181,452,135 (GRCm39)	missense	probably damaging	1.00
R9700:Myt1	UTSW	2	181,452,177 (GRCm39)	missense	probably damaging	1.00
RF006:Myt1	UTSW	2	181,439,566 (GRCm39)	missense	probably damaging	1.00
Z1177:Myt1	UTSW	2	181,449,395 (GRCm39)	missense	probably damaging	1.00
Z1177:Myt1	UTSW	2	181,438,955 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GAGGATTCCGTCTCAGTAGC -3'
(R):5'- CCAAAGATAACATTTGGAGCTGC -3'

Sequencing Primer
(F):5'- GTCTCAGTAGCTAAGTTGAGCCC -3'
(R):5'- AGATAACATTTGGAGCTGCTTCCTC -3'

Posted On

2015-10-08