Mutagenetix > Incidental Mutation

Incidental Mutation 'R4819:Zfp935'

370071

Institutional Source

Beutler Lab

Gene Symbol

Zfp935

Ensembl Gene

ENSMUSG00000055228

Gene Name

zinc finger protein 935

Synonyms

8430426H19Rik

MMRRC Submission

042000-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.114) question?

Stock #

R4819 (G1)

Quality Score

224

Status

Not validated

Chromosome

Chromosomal Location

62453715-62558511 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 62454417 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 323 (H323L)

Ref Sequence

ENSEMBL: ENSMUSP00000152662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000221747] [ENSMUST00000223247]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221260

Predicted Effect

probably damaging
Transcript: ENSMUST00000221747
AA Change: H323L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221951

Predicted Effect

probably benign
Transcript: ENSMUST00000223247

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.5%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,290,421 (GRCm38)	N761K	possibly damaging	Het
Adgrf3	T	A	5: 30,198,444 (GRCm38)	L444F	possibly damaging	Het
Akap8	G	A	17: 32,312,305 (GRCm38)	R378W	probably damaging	Het
Amotl2	C	T	9: 102,730,071 (GRCm38)	R693W	probably damaging	Het
As3mt	G	T	19: 46,707,529 (GRCm38)		probably benign	Het
Atp6v1e2	A	G	17: 86,944,538 (GRCm38)	V144A	probably benign	Het
Bfar	C	T	16: 13,687,467 (GRCm38)	Q114*	probably null	Het
Casd1	C	T	6: 4,621,225 (GRCm38)	A261V	probably damaging	Het
Cd177	A	T	7: 24,752,271 (GRCm38)	I440K	probably damaging	Het
Cfap54	G	T	10: 92,836,477 (GRCm38)	Y2910*	probably null	Het
Csl	A	G	10: 99,758,082 (GRCm38)	F374L	possibly damaging	Het
Dctn1	G	A	6: 83,190,519 (GRCm38)	R275H	probably damaging	Het
Derl3	A	G	10: 75,893,879 (GRCm38)		probably null	Het
Dst	A	G	1: 33,968,835 (GRCm38)	I117V	probably benign	Het
Edc3	T	A	9: 57,748,397 (GRCm38)	C477S	possibly damaging	Het
Efs	T	C	14: 54,917,153 (GRCm38)	E450G	probably damaging	Het
Fcrla	T	A	1: 170,920,939 (GRCm38)	I212F	probably damaging	Het
Fsip2	A	G	2: 82,988,442 (GRCm38)	I4840V	probably benign	Het
Gpam	T	A	19: 55,078,341 (GRCm38)	I581F	probably benign	Het
Greb1l	A	G	18: 10,458,358 (GRCm38)	D45G	probably damaging	Het
Heca	A	G	10: 17,908,072 (GRCm38)	Y478H	probably damaging	Het
Hspa9	C	T	18: 34,939,388 (GRCm38)	M561I	probably damaging	Het
Hyal6	T	A	6: 24,734,966 (GRCm38)	Y299*	probably null	Het
Iho1	A	G	9: 108,406,678 (GRCm38)	V189A	probably benign	Het
Ik	T	A	18: 36,753,257 (GRCm38)		probably null	Het
Khsrp	A	G	17: 57,023,360 (GRCm38)	S582P	possibly damaging	Het
Kif18a	A	G	2: 109,292,126 (GRCm38)	D182G	probably damaging	Het
Krt2	T	C	15: 101,811,544 (GRCm38)	T564A	unknown	Het
Lig4	A	G	8: 9,971,885 (GRCm38)	S632P	probably benign	Het
Med1	C	T	11: 98,155,432 (GRCm38)		probably benign	Het
Mgat3	T	A	15: 80,212,349 (GRCm38)	I459N	probably damaging	Het
Mkln1	A	T	6: 31,474,486 (GRCm38)	Q454L	probably benign	Het
Mn1	A	G	5: 111,419,937 (GRCm38)	E591G	possibly damaging	Het
Myo5c	T	A	9: 75,292,202 (GRCm38)	L1364Q	probably damaging	Het
Oas1d	T	C	5: 120,915,717 (GRCm38)	V80A	probably damaging	Het
Obscn	A	T	11: 59,038,848 (GRCm38)	D5180E	probably damaging	Het
Pax6	G	A	2: 105,692,277 (GRCm38)		probably null	Het
Pcdh15	A	C	10: 74,324,389 (GRCm38)	N446T	probably damaging	Het
Pcnx2	A	T	8: 125,855,230 (GRCm38)	F922L	probably benign	Het
Ptpn4	G	T	1: 119,659,850 (GRCm38)	T921K	probably benign	Het
Selenov	A	G	7: 28,290,321 (GRCm38)		probably null	Het
Tmem100	A	G	11: 90,035,445 (GRCm38)	T33A	probably benign	Het
Tmem59	C	T	4: 107,187,681 (GRCm38)	Q66*	probably null	Het
Trav21-dv12	T	A	14: 53,876,613 (GRCm38)	Y63*	probably null	Het
Trim66	G	T	7: 109,457,586 (GRCm38)	H1121Q	probably damaging	Het
Trim80	A	G	11: 115,447,943 (GRCm38)	Y533C	probably damaging	Het
Ttc17	G	A	2: 94,364,610 (GRCm38)	P520L	probably damaging	Het
Ttn	A	T	2: 76,791,749 (GRCm38)	V15483E	probably damaging	Het
Vinac1	G	T	2: 129,040,801 (GRCm38)	N98K	probably damaging	Het
Zdhhc1	CGGGGG	CGGGGGG	8: 105,483,744 (GRCm38)		probably null	Het
Zfhx4	C	A	3: 5,403,914 (GRCm38)	T3069K	probably benign	Het
Zfp281	A	G	1: 136,625,710 (GRCm38)	H142R	probably benign	Het
Zfp462	G	T	4: 55,060,044 (GRCm38)	R1190L	probably damaging	Het

Other mutations in Zfp935

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00791:Zfp935	APN	13	62,454,464 (GRCm38)	missense	probably benign	0.33
IGL02169:Zfp935	APN	13	62,456,931 (GRCm38)	critical splice donor site	probably null
IGL02570:Zfp935	APN	13	62,456,978 (GRCm38)	missense	probably damaging	1.00
IGL02756:Zfp935	APN	13	62,454,887 (GRCm38)	nonsense	probably null
IGL03338:Zfp935	APN	13	62,454,433 (GRCm38)	missense	probably benign	0.11
R1252:Zfp935	UTSW	13	62,454,541 (GRCm38)	missense	probably damaging	1.00
R1503:Zfp935	UTSW	13	62,455,137 (GRCm38)	missense	possibly damaging	0.65
R2425:Zfp935	UTSW	13	62,455,108 (GRCm38)	missense	probably benign	0.02
R3720:Zfp935	UTSW	13	62,455,032 (GRCm38)	nonsense	probably null
R5391:Zfp935	UTSW	13	62,454,818 (GRCm38)	nonsense	probably null
R6253:Zfp935	UTSW	13	62,454,871 (GRCm38)	missense	probably benign	0.02
R6742:Zfp935	UTSW	13	62,454,479 (GRCm38)	missense	probably damaging	1.00
R9447:Zfp935	UTSW	13	62,455,028 (GRCm38)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- TAACTGTGTTGTGAAAAGGCTG -3'
(R):5'- TCCCAATACGTTCATCTCCAAAT -3'

Sequencing Primer
(F):5'- TGTGAAAAGGCTGTCACATTG -3'
(R):5'- TACTGGAGAGAAACCCTTCAAATGTG -3'

Posted On

2016-02-04