|Institutional Source||Beutler Lab|
|Gene Name||T cell immunoreceptor with Ig and ITIM domains|
|Is this an essential gene?||Probably non essential (E-score: 0.070)|
|Stock #||R0446 (G1)|
|Chromosomal Location||43648867-43664146 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||G to T at 43662271 bp|
|Amino Acid Change||Asparagine to Lysine at position 33 (N33K)|
|Ref Sequence||ENSEMBL: ENSMUSP00000093770 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000096065]|
|Predicted Effect||probably damaging
AA Change: N33K
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: N33K
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased T cell proliferation, antigen presenting cell stimuation of T cell proliferation, and susceptibility to EAE. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Tigit||
(F):5'- ACTGAAGACTGAAGCGACATGCC -3'
(R):5'- AGGAAGCACAGCTCTGACTGATGG -3'
(F):5'- GACATGCCACCCCAGGTC -3'
(R):5'- CTCTGGAAATCAATGATGGTCTC -3'