Mutagenetix > Incidental Mutation

Incidental Mutation 'R5502:Fyb2'

430654

Institutional Source

Beutler Lab

Gene Symbol

Fyb2

Ensembl Gene

ENSMUSG00000078612

Gene Name

FYN binding protein 2

Synonyms

1700024P16Rik

MMRRC Submission

043063-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R5502 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

104770653-104874060 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 104802521 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 141 (Q141L)

Ref Sequence

ENSEMBL: ENSMUSP00000102415 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106803] [ENSMUST00000106804]

AlphaFold

A2A995

Predicted Effect

probably damaging
Transcript: ENSMUST00000106803
AA Change: Q141L

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000102415
Gene: ENSMUSG00000078612
AA Change: Q141L

Domain	Start	End	E-Value	Type
low complexity region	120	132	N/A	INTRINSIC
low complexity region	340	349	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	537	549	N/A	INTRINSIC
low complexity region	567	578	N/A	INTRINSIC
low complexity region	682	697	N/A	INTRINSIC
SH3	735	791	3.82e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106804
AA Change: Q77L

PolyPhen 2 Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000102416
Gene: ENSMUSG00000078612
AA Change: Q77L

Domain	Start	End	E-Value	Type
low complexity region	56	68	N/A	INTRINSIC
low complexity region	276	285	N/A	INTRINSIC
low complexity region	378	395	N/A	INTRINSIC
low complexity region	473	485	N/A	INTRINSIC
low complexity region	503	514	N/A	INTRINSIC
low complexity region	618	633	N/A	INTRINSIC
SH3	671	727	3.82e0	SMART

Meta Mutation Damage Score

0.0906

Coding Region Coverage

1x: 98.2%
3x: 97.3%
10x: 95.0%
20x: 90.2%

Validation Efficiency

98% (84/86)

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
Abcc8	A	G	7: 45,758,262 (GRCm39)	I1268T	probably benign	Het
Accsl	C	T	2: 93,687,289 (GRCm39)		probably null	Het
Actmap	A	G	7: 26,896,542 (GRCm39)	D35G	possibly damaging	Het
Ank3	A	T	10: 69,756,291 (GRCm39)	I842F	probably benign	Het
Bbs9	T	A	9: 22,415,370 (GRCm39)	L98Q	probably damaging	Het
Bin2	A	G	15: 100,543,286 (GRCm39)	V299A	probably benign	Het
Cabp4	G	A	19: 4,181,228 (GRCm39)		probably benign	Het
Ces2f	A	T	8: 105,679,155 (GRCm39)	H324L	possibly damaging	Het
Chd4	G	A	6: 125,082,239 (GRCm39)	R576Q	possibly damaging	Het
Cndp1	A	G	18: 84,650,138 (GRCm39)	V185A	possibly damaging	Het
Cntn3	A	C	6: 102,242,295 (GRCm39)	V450G	possibly damaging	Het
Col5a2	C	A	1: 45,419,286 (GRCm39)	G1265W	probably damaging	Het
Corin	G	A	5: 72,473,449 (GRCm39)	Q754*	probably null	Het
Cyp4a10	A	G	4: 115,382,702 (GRCm39)	N291S	probably benign	Het
Dap3	T	C	3: 88,832,633 (GRCm39)	Y353C	probably damaging	Het
Disp1	A	G	1: 182,869,450 (GRCm39)	V990A	probably damaging	Het
Dock9	A	T	14: 121,847,594 (GRCm39)		probably null	Het
Dop1b	T	A	16: 93,590,114 (GRCm39)	V179E	probably benign	Het
Eps15l1	A	T	8: 73,132,836 (GRCm39)		probably null	Het
Gemin4	A	T	11: 76,104,227 (GRCm39)	L178*	probably null	Het
Gm15446	A	T	5: 110,088,364 (GRCm39)	K25*	probably null	Het
Gm17067	A	T	7: 42,357,843 (GRCm39)	C220S	probably damaging	Het
Gm4868	A	G	5: 125,925,042 (GRCm39)		noncoding transcript	Het
Golga4	GT	GTT	9: 118,388,125 (GRCm39)		probably null	Het
Gria2	T	C	3: 80,614,252 (GRCm39)	N596S	probably damaging	Het
Hoxb4	A	G	11: 96,211,057 (GRCm39)	D219G	probably damaging	Het
Htr1b	T	A	9: 81,513,854 (GRCm39)	Q251L	possibly damaging	Het
Ibtk	A	C	9: 85,602,916 (GRCm39)	S696R	probably benign	Het
Ide	C	A	19: 37,307,855 (GRCm39)	K52N	unknown	Het
Incenp	C	A	19: 9,870,728 (GRCm39)	L300F	unknown	Het
Ino80	T	C	2: 119,232,877 (GRCm39)	Y1147C	probably damaging	Het
Mme	T	A	3: 63,207,702 (GRCm39)	Y49*	probably null	Het
Mmp15	A	T	8: 96,094,812 (GRCm39)	T229S	possibly damaging	Het
Mtmr4	A	T	11: 87,504,904 (GRCm39)	N1133I	probably damaging	Het
Mycbp2	C	T	14: 103,411,250 (GRCm39)	G284R	probably damaging	Het
Myo3a	T	C	2: 22,448,381 (GRCm39)	I52T	probably damaging	Het
Nat8f5	G	T	6: 85,794,635 (GRCm39)	F108L	probably damaging	Het
Nbeal1	T	A	1: 60,350,158 (GRCm39)	H2402Q	probably damaging	Het
Nexn	T	A	3: 151,943,941 (GRCm39)	E331D	probably damaging	Het
Nktr	C	T	9: 121,577,672 (GRCm39)		probably benign	Het
Oaz3	T	C	3: 94,342,392 (GRCm39)	D88G	probably damaging	Het
Or1n1	T	C	2: 36,750,282 (GRCm39)	Y26C	probably damaging	Het
Or4l1	A	G	14: 50,166,993 (GRCm39)	Y3H	probably benign	Het
Pcdhb9	A	T	18: 37,534,656 (GRCm39)	T217S	possibly damaging	Het
Pcdhga2	A	T	18: 37,803,605 (GRCm39)	D483V	possibly damaging	Het
Pde5a	G	T	3: 122,596,681 (GRCm39)	G456V	probably damaging	Het
Qser1	T	C	2: 104,616,919 (GRCm39)	T1298A	probably benign	Het
Rapgef5	T	C	12: 117,685,064 (GRCm39)	V303A	probably damaging	Het
Rbbp6	T	G	7: 122,587,947 (GRCm39)	M267R	probably damaging	Het
Rfx8	C	A	1: 39,722,113 (GRCm39)	V291F	probably damaging	Het
Rnf121	T	C	7: 101,672,555 (GRCm39)	K276R	probably null	Het
Rtca	G	T	3: 116,282,931 (GRCm39)	Y352*	probably null	Het
Rusf1	C	T	7: 127,884,308 (GRCm39)	V225M	probably damaging	Het
Sacs	T	C	14: 61,443,549 (GRCm39)	V1865A	probably damaging	Het
Sclt1	C	T	3: 41,611,710 (GRCm39)	E521K	probably benign	Het
Setd1a	T	C	7: 127,396,420 (GRCm39)		probably null	Het
Slc37a4	G	T	9: 44,313,394 (GRCm39)	V337L	probably benign	Het
Slc5a9	G	T	4: 111,750,366 (GRCm39)	S79*	probably null	Het
Snrpd2	T	C	7: 18,885,247 (GRCm39)	V36A	probably benign	Het
Spata31d1b	A	T	13: 59,864,486 (GRCm39)	N545Y	probably damaging	Het
Sstr4	CGAGGAGGAGGAGGA	CGAGGAGGAGGAGGAGGA	2: 148,237,471 (GRCm39)		probably benign	Het
St7	G	A	6: 17,834,673 (GRCm39)	V98I	possibly damaging	Het
Strip2	A	T	6: 29,927,623 (GRCm39)	I223F	probably benign	Het
Syn2	A	C	6: 115,255,313 (GRCm39)	N542H	possibly damaging	Het
Tbc1d2b	T	C	9: 90,109,496 (GRCm39)	T327A	probably benign	Het
Timm44	A	T	8: 4,319,992 (GRCm39)	F59I	possibly damaging	Het
Tmem41b	A	T	7: 109,581,970 (GRCm39)	C44*	probably null	Het
Tob1	ACAGCAGCAGCAGCAGCAGCAGCAGCA	ACAGCAGCAGCAGCAGCAGCAGCA	11: 94,105,278 (GRCm39)		probably benign	Het
Tusc3	A	T	8: 39,597,947 (GRCm39)	K188*	probably null	Het
Usp39	T	A	6: 72,305,670 (GRCm39)	Q371L	probably benign	Het
Vmn2r13	G	T	5: 109,321,580 (GRCm39)	N372K	probably damaging	Het
Zfp961	T	A	8: 72,721,903 (GRCm39)	Y139N	probably damaging	Het

Other mutations in Fyb2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00664:Fyb2	APN	4	104,872,913 (GRCm39)	missense	probably damaging	1.00
IGL01155:Fyb2	APN	4	104,856,583 (GRCm39)	missense	probably benign	0.00
IGL01632:Fyb2	APN	4	104,853,008 (GRCm39)	missense	probably benign
IGL01746:Fyb2	APN	4	104,802,404 (GRCm39)	missense	probably benign	0.01
IGL02381:Fyb2	APN	4	104,805,863 (GRCm39)	splice site	probably benign
IGL02590:Fyb2	APN	4	104,836,250 (GRCm39)	missense	probably damaging	1.00
IGL02885:Fyb2	APN	4	104,861,118 (GRCm39)	missense	probably damaging	0.99
IGL03114:Fyb2	APN	4	104,852,975 (GRCm39)	missense	probably damaging	0.97
IGL03189:Fyb2	APN	4	104,872,939 (GRCm39)	missense	probably damaging	1.00
IGL03231:Fyb2	APN	4	104,843,460 (GRCm39)	nonsense	probably null
R0076:Fyb2	UTSW	4	104,802,661 (GRCm39)	missense	possibly damaging	0.46
R0662:Fyb2	UTSW	4	104,852,895 (GRCm39)	missense	possibly damaging	0.46
R0723:Fyb2	UTSW	4	104,873,063 (GRCm39)	missense	probably benign	0.00
R1216:Fyb2	UTSW	4	104,852,903 (GRCm39)	missense	possibly damaging	0.86
R1672:Fyb2	UTSW	4	104,808,059 (GRCm39)	missense	probably benign	0.10
R1710:Fyb2	UTSW	4	104,861,113 (GRCm39)	missense	probably damaging	1.00
R1900:Fyb2	UTSW	4	104,802,652 (GRCm39)	missense	probably benign	0.06
R1965:Fyb2	UTSW	4	104,770,846 (GRCm39)	missense	probably benign	0.00
R2106:Fyb2	UTSW	4	104,802,769 (GRCm39)	missense	probably benign	0.01
R5191:Fyb2	UTSW	4	104,852,994 (GRCm39)	missense	possibly damaging	0.88
R5236:Fyb2	UTSW	4	104,805,957 (GRCm39)	missense	probably benign	0.00
R5277:Fyb2	UTSW	4	104,872,876 (GRCm39)	missense	probably damaging	1.00
R5769:Fyb2	UTSW	4	104,872,841 (GRCm39)	missense	probably damaging	1.00
R5769:Fyb2	UTSW	4	104,870,518 (GRCm39)	missense	probably damaging	1.00
R6167:Fyb2	UTSW	4	104,802,661 (GRCm39)	missense	possibly damaging	0.46
R6169:Fyb2	UTSW	4	104,857,713 (GRCm39)	missense	probably benign	0.16
R6371:Fyb2	UTSW	4	104,852,975 (GRCm39)	missense	probably damaging	0.97
R6582:Fyb2	UTSW	4	104,802,739 (GRCm39)	missense	probably benign	0.00
R6713:Fyb2	UTSW	4	104,847,432 (GRCm39)	missense	probably benign	0.16
R6719:Fyb2	UTSW	4	104,867,656 (GRCm39)	missense	probably benign	0.07
R7484:Fyb2	UTSW	4	104,870,499 (GRCm39)	missense	probably benign	0.01
R7534:Fyb2	UTSW	4	104,856,545 (GRCm39)	nonsense	probably null
R7590:Fyb2	UTSW	4	104,802,443 (GRCm39)	missense	probably benign	0.01
R7699:Fyb2	UTSW	4	104,867,651 (GRCm39)	missense	probably benign	0.07
R7700:Fyb2	UTSW	4	104,867,651 (GRCm39)	missense	probably benign	0.07
R8041:Fyb2	UTSW	4	104,857,681 (GRCm39)	missense	possibly damaging	0.82
R8298:Fyb2	UTSW	4	104,806,028 (GRCm39)	missense	probably damaging	0.99
R8300:Fyb2	UTSW	4	104,857,689 (GRCm39)	missense	probably damaging	1.00
R8755:Fyb2	UTSW	4	104,861,086 (GRCm39)	missense	unknown
R8817:Fyb2	UTSW	4	104,802,652 (GRCm39)	missense	probably benign	0.06
R8873:Fyb2	UTSW	4	104,856,538 (GRCm39)	missense	probably damaging	1.00
R8914:Fyb2	UTSW	4	104,857,700 (GRCm39)	missense	probably benign	0.09
R9224:Fyb2	UTSW	4	104,853,105 (GRCm39)	missense	probably benign	0.44
R9434:Fyb2	UTSW	4	104,847,534 (GRCm39)	missense	probably damaging	0.99
R9512:Fyb2	UTSW	4	104,853,100 (GRCm39)	missense	probably benign	0.00
R9589:Fyb2	UTSW	4	104,872,833 (GRCm39)	missense	probably damaging	1.00
R9634:Fyb2	UTSW	4	104,856,579 (GRCm39)	nonsense	probably null
R9758:Fyb2	UTSW	4	104,802,961 (GRCm39)	missense	probably benign	0.01
X0018:Fyb2	UTSW	4	104,802,407 (GRCm39)	missense	probably benign	0.04
Z1176:Fyb2	UTSW	4	104,770,857 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AATGCTGCACCTCTTCCAG -3'
(R):5'- AGTGGAGCTGGGGACATTTC -3'

Sequencing Primer
(F):5'- CTTCCAGGGCCAATGAGGTTTC -3'
(R):5'- CCCAGTTCCAAAGCTTGTATCTGAAG -3'

Posted On

2016-10-05