Mutagenetix > Incidental Mutation

Incidental Mutation 'R8420:Or4m1'

653161

Institutional Source

Beutler Lab

Gene Symbol

Or4m1

Ensembl Gene

ENSMUSG00000045306

Gene Name

olfactory receptor family 4 subfamily M member 1

Synonyms

Olfr734, GA_x6K02T2PMLR-6013665-6012724, MOR242-1

MMRRC Submission

067773-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.495) question?

Stock #

R8420 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

50557293-50558325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 50558233 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 20 (T20A)

Ref Sequence

ENSEMBL: ENSMUSP00000150732 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050928] [ENSMUST00000217152]

AlphaFold

Q8VFT4

Predicted Effect

probably benign
Transcript: ENSMUST00000050928
AA Change: T20A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000057376
Gene: ENSMUSG00000045306
AA Change: T20A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.3e-39	PFAM
Pfam:7tm_1	41	302	4.1e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217152
AA Change: T20A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	A	G	11: 119,937,746 (GRCm39)	S11P	unknown	Het
Adrb2	T	C	18: 62,312,004 (GRCm39)	T274A	probably damaging	Het
Aph1b	A	G	9: 66,701,503 (GRCm39)	S45P	probably damaging	Het
Atp9b	A	T	18: 80,887,806 (GRCm39)	D321E		Het
C1qbp	A	G	11: 70,869,543 (GRCm39)	V180A	possibly damaging	Het
C4b	G	T	17: 34,953,513 (GRCm39)	A990D	probably damaging	Het
Ccdc80	T	A	16: 44,915,612 (GRCm39)	S123T	possibly damaging	Het
Cdh18	A	C	15: 23,474,138 (GRCm39)	E669D	possibly damaging	Het
Ceacam3	T	A	7: 16,895,608 (GRCm39)	V526D		Het
Cenpf	C	T	1: 189,404,782 (GRCm39)	C349Y	probably damaging	Het
Dmrt2	T	C	19: 25,655,379 (GRCm39)	V326A	probably damaging	Het
Dock9	T	C	14: 121,783,454 (GRCm39)	Q2023R	probably damaging	Het
Exd2	A	G	12: 80,522,771 (GRCm39)	R77G	probably benign	Het
Exph5	A	T	9: 53,287,148 (GRCm39)	K1410*	probably null	Het
Eya2	A	G	2: 165,608,988 (GRCm39)	T443A	probably damaging	Het
Fam135a	G	A	1: 24,067,569 (GRCm39)	T1100M	probably benign	Het
Foxp2	G	T	6: 15,403,866 (GRCm39)	R381L	unknown	Het
Grm7	T	G	6: 111,057,315 (GRCm39)	V305G	probably benign	Het
Gzma	G	A	13: 113,237,464 (GRCm39)	R8W	probably benign	Het
H6pd	T	C	4: 150,066,133 (GRCm39)	E759G	probably benign	Het
Ift27	A	G	15: 78,048,391 (GRCm39)	V154A	probably benign	Het
Kif1a	A	T	1: 92,950,141 (GRCm39)	S1429T	probably benign	Het
Lrrn1	T	A	6: 107,546,294 (GRCm39)	D697E	probably benign	Het
Mcrs1	A	T	15: 99,141,575 (GRCm39)	I387N	probably damaging	Het
Muc16	T	C	9: 18,448,807 (GRCm39)	T7675A	probably damaging	Het
Naf1	GCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAACTGGGATGCGGGCGGAAGACCACCACCGCCGCCAGCCCCGAACTCGGATCCCGGCGGAAGACC	GCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAACTGGGATGCGGGCGGAAGACCACCACCGCCGCCAGCCCCGAACTCGGATCCCGGCGGAAGACC	8: 67,313,200 (GRCm39)		probably benign	Het
Nid1	C	A	13: 13,612,416 (GRCm39)	L44I	possibly damaging	Het
Or2f2	A	T	6: 42,767,644 (GRCm39)	T224S	possibly damaging	Het
Or5h27	A	G	16: 59,006,117 (GRCm39)	I243T	unknown	Het
Or8k1	T	A	2: 86,047,457 (GRCm39)	E199V	probably damaging	Het
Pde11a	C	T	2: 75,889,354 (GRCm39)	D707N	probably damaging	Het
Pigf	A	T	17: 87,327,910 (GRCm39)	L119*	probably null	Het
Pkd1l1	A	T	11: 8,820,277 (GRCm39)	C1679*	probably null	Het
Prnp	A	G	2: 131,778,669 (GRCm39)	N107S	probably benign	Het
Ptgfr	C	T	3: 151,541,053 (GRCm39)	V152M	possibly damaging	Het
Rap2b	A	G	3: 61,271,805 (GRCm39)		probably benign	Het
Rtn4	A	G	11: 29,657,300 (GRCm39)	T485A	probably damaging	Het
Sec23b	A	G	2: 144,401,234 (GRCm39)	T32A	probably benign	Het
Skint5	A	T	4: 113,437,679 (GRCm39)		probably null	Het
Slc13a5	A	G	11: 72,148,210 (GRCm39)	L275P	probably damaging	Het
Slc20a1	C	T	2: 129,041,784 (GRCm39)	A49V	probably damaging	Het
Slc35g2	A	G	9: 100,435,224 (GRCm39)	I149T	probably benign	Het
Syk	T	A	13: 52,778,763 (GRCm39)	I283K	probably benign	Het
Taf7l2	T	C	10: 115,948,440 (GRCm39)	Y362C	probably benign	Het
Tnfsf13b	G	A	8: 10,056,795 (GRCm39)		probably benign	Het
Ubr1	A	G	2: 120,701,476 (GRCm39)	V1592A	probably benign	Het
Vopp1	T	C	6: 57,739,379 (GRCm39)	*123W	probably null	Het
Xpo4	T	A	14: 57,841,913 (GRCm39)	Q467H	probably damaging	Het
Zdhhc22	A	C	12: 87,035,143 (GRCm39)	V103G	possibly damaging	Het
Zfp868	A	G	8: 70,064,160 (GRCm39)	S392P	probably damaging	Het

Other mutations in Or4m1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Or4m1	APN	14	50,557,732 (GRCm39)	missense	probably damaging	0.96
IGL01285:Or4m1	APN	14	50,557,713 (GRCm39)	missense	possibly damaging	0.88
IGL02106:Or4m1	APN	14	50,557,617 (GRCm39)	missense	probably damaging	1.00
IGL02313:Or4m1	APN	14	50,557,473 (GRCm39)	missense	probably damaging	0.99
IGL03125:Or4m1	APN	14	50,558,149 (GRCm39)	missense	probably benign	0.01
R0276:Or4m1	UTSW	14	50,557,636 (GRCm39)	missense	probably benign	0.23
R0547:Or4m1	UTSW	14	50,557,575 (GRCm39)	missense	probably benign	0.06
R0567:Or4m1	UTSW	14	50,558,115 (GRCm39)	missense	probably damaging	0.99
R0927:Or4m1	UTSW	14	50,558,186 (GRCm39)	nonsense	probably null
R1506:Or4m1	UTSW	14	50,557,941 (GRCm39)	missense	probably benign	0.00
R4032:Or4m1	UTSW	14	50,557,767 (GRCm39)	missense	possibly damaging	0.91
R5179:Or4m1	UTSW	14	50,557,993 (GRCm39)	nonsense	probably null
R5401:Or4m1	UTSW	14	50,557,566 (GRCm39)	missense	probably damaging	1.00
R6240:Or4m1	UTSW	14	50,558,043 (GRCm39)	missense	probably benign	0.00
R7752:Or4m1	UTSW	14	50,557,573 (GRCm39)	missense	probably damaging	1.00
R7901:Or4m1	UTSW	14	50,557,573 (GRCm39)	missense	probably damaging	1.00
R8034:Or4m1	UTSW	14	50,558,023 (GRCm39)	missense	probably damaging	1.00
R8260:Or4m1	UTSW	14	50,557,615 (GRCm39)	missense	probably benign	0.09
R9056:Or4m1	UTSW	14	50,557,999 (GRCm39)	missense	probably damaging	0.98
R9128:Or4m1	UTSW	14	50,558,214 (GRCm39)	missense	probably benign	0.08
R9618:Or4m1	UTSW	14	50,557,760 (GRCm39)	nonsense	probably null
R9659:Or4m1	UTSW	14	50,558,181 (GRCm39)	missense	probably benign	0.10
R9788:Or4m1	UTSW	14	50,558,181 (GRCm39)	missense	probably benign	0.10
X0064:Or4m1	UTSW	14	50,557,511 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGAAGAGTTGAGCAATACACCC -3'
(R):5'- CCCCTGTGTAATCTATTAATGATGC -3'

Sequencing Primer
(F):5'- TACAAGCATCTTAGGCGCTG -3'
(R):5'- TCCATAGCCATAATGACAAAT -3'

Posted On

2020-10-20