Mutagenetix > Incidental Mutation

Incidental Mutation 'R8866:Or1e32'

675947

Institutional Source

Beutler Lab

Gene Symbol

Or1e32

Ensembl Gene

ENSMUSG00000061984

Gene Name

olfactory receptor family 1 subfamily E member 32

Synonyms

GA_x6K02T2P1NL-3966976-3966038, Olfr392, MOR135-9

MMRRC Submission

068742-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8866 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73704968-73707703 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73705237 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 224 (S224T)

Ref Sequence

ENSEMBL: ENSMUSP00000113118 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000118463] [ENSMUST00000144724]

AlphaFold

Q7TRX6

Predicted Effect

probably damaging
Transcript: ENSMUST00000118463
AA Change: S224T

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000113118
Gene: ENSMUSG00000061984
AA Change: S224T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.5e-58	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.6e-6	PFAM
Pfam:7tm_1	41	290	1.2e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000144724
AA Change: S224T

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000123649
Gene: ENSMUSG00000061984
AA Change: S224T

Domain	Start	End	E-Value	Type
Pfam:7tm_1	41	116	2e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2	C	T	3: 59,914,511 (GRCm39)	T38I	probably benign	Het
Arhgap1	G	T	2: 91,499,744 (GRCm39)	S293I	probably benign	Het
Arhgap45	C	A	10: 79,853,750 (GRCm39)	P88Q	probably damaging	Het
Atg9a	A	C	1: 75,161,867 (GRCm39)	F560V	probably damaging	Het
Btaf1	T	A	19: 36,935,901 (GRCm39)	N230K	probably benign	Het
Ccne1	A	C	7: 37,800,046 (GRCm39)	H179Q	probably benign	Het
Dbr1	T	A	9: 99,460,497 (GRCm39)	L109*	probably null	Het
Dchs1	G	T	7: 105,404,597 (GRCm39)	N2648K	probably benign	Het
Ddx47	A	G	6: 135,000,356 (GRCm39)	K451E	probably benign	Het
Dlgap1	A	T	17: 70,823,435 (GRCm39)	H140L	probably damaging	Het
Dnah11	T	A	12: 118,156,107 (GRCm39)	H181L	probably benign	Het
Dync1h1	T	C	12: 110,602,333 (GRCm39)	C2074R	probably damaging	Het
Ell2	A	T	13: 75,917,793 (GRCm39)	Q574H	probably damaging	Het
Eloa	A	C	4: 135,737,538 (GRCm39)		probably null	Het
Epha7	A	G	4: 28,821,614 (GRCm39)	I260V	probably benign	Het
Fbp2	T	C	13: 62,989,709 (GRCm39)	K199R	probably benign	Het
Fetub	A	T	16: 22,758,321 (GRCm39)	E368V	possibly damaging	Het
Fsip2	A	G	2: 82,810,521 (GRCm39)	Q2280R	probably benign	Het
Fstl4	A	G	11: 52,963,233 (GRCm39)	E205G	possibly damaging	Het
Gm5592	A	T	7: 40,938,246 (GRCm39)	R509S	possibly damaging	Het
Gpld1	A	G	13: 25,170,890 (GRCm39)	T794A	probably benign	Het
Gsk3b	A	G	16: 38,004,900 (GRCm39)	Y157C	probably damaging	Het
Hgs	A	G	11: 120,360,464 (GRCm39)	N54S	probably benign	Het
Hydin	G	A	8: 111,308,779 (GRCm39)	V4022M	possibly damaging	Het
Kank1	T	C	19: 25,388,702 (GRCm39)	S792P	possibly damaging	Het
Kctd4	A	G	14: 76,200,465 (GRCm39)	I145M	probably benign	Het
Klra5	A	T	6: 129,880,533 (GRCm39)	W212R	probably damaging	Het
Lrp6	A	T	6: 134,445,785 (GRCm39)	N1009K	probably benign	Het
Lrrc10	T	A	10: 116,881,858 (GRCm39)	N177K	probably damaging	Het
Lrrc39	T	A	3: 116,363,790 (GRCm39)	I121N	probably damaging	Het
Mov10l1	A	T	15: 88,896,169 (GRCm39)	D671V	probably benign	Het
Ndel1	A	G	11: 68,734,645 (GRCm39)		probably null	Het
Or10g3	A	T	14: 52,610,196 (GRCm39)	Y105N	probably damaging	Het
Or1e27-ps1	A	G	11: 73,555,825 (GRCm39)	Y130C	probably damaging	Het
Or51a43	A	T	7: 103,718,119 (GRCm39)	S40T	probably damaging	Het
Otop2	A	G	11: 115,220,354 (GRCm39)	D398G	probably benign	Het
Pkd1	T	C	17: 24,792,051 (GRCm39)	V1246A	probably damaging	Het
Polr3b	T	A	10: 84,531,555 (GRCm39)	D810E	probably benign	Het
Ppl	T	A	16: 4,920,211 (GRCm39)	T395S	probably benign	Het
Ptprm	G	A	17: 67,116,630 (GRCm39)	A883V	probably benign	Het
Rapgef6	G	A	11: 54,443,700 (GRCm39)		probably null	Het
Rbm12	A	T	2: 155,938,693 (GRCm39)	Y526*	probably null	Het
Rbm15b	C	T	9: 106,763,595 (GRCm39)	R191Q	probably benign	Het
Rsf1	GCGGCGGCG	GCGGCGGCGACGGCGGCG	7: 97,229,120 (GRCm39)		probably benign	Het
Rxylt1	A	G	10: 121,924,953 (GRCm39)	Y250H	probably damaging	Het
Serpinb6c	A	T	13: 34,083,309 (GRCm39)	I35N	probably damaging	Het
Setd4	A	G	16: 93,386,961 (GRCm39)	F246L	probably damaging	Het
Shank2	A	G	7: 143,964,986 (GRCm39)	S865G	probably benign	Het
Skint6	T	C	4: 112,711,650 (GRCm39)	N956D	probably benign	Het
Slc30a3	C	A	5: 31,245,325 (GRCm39)	V332F	possibly damaging	Het
Smg1	A	G	7: 117,806,122 (GRCm39)	S240P	unknown	Het
Tank	T	A	2: 61,409,005 (GRCm39)	S7R	probably benign	Het
Tecpr1	T	C	5: 144,153,117 (GRCm39)	E204G	possibly damaging	Het
Tmem74	A	G	15: 43,730,231 (GRCm39)	S271P	probably damaging	Het
Tor4a	T	C	2: 25,084,965 (GRCm39)	M313V	probably benign	Het
Tpd52l2	A	G	2: 181,154,861 (GRCm39)	D192G	probably damaging	Het
Trim29	T	C	9: 43,222,945 (GRCm39)	V258A	probably damaging	Het
Ttc39c	A	T	18: 12,831,003 (GRCm39)	S288C	probably benign	Het
Ttn	T	C	2: 76,592,675 (GRCm39)	E20819G	probably damaging	Het
Twnk	C	T	19: 45,000,272 (GRCm39)	Q663*	probably null	Het
Ucn	T	A	5: 31,295,857 (GRCm39)	Q3L	probably benign	Het
Unc80	G	A	1: 66,629,388 (GRCm39)	G1295E	probably benign	Het
Usp9y	C	T	Y: 1,395,758 (GRCm39)	R492H	probably damaging	Het
Vars1	T	C	17: 35,234,620 (GRCm39)	S1150P	probably benign	Het
Vwa5b1	T	G	4: 138,327,628 (GRCm39)	E316A	probably damaging	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zfp839	A	G	12: 110,834,848 (GRCm39)	E701G	probably benign	Het
Zfp931	A	T	2: 177,710,178 (GRCm39)	C69*	probably null	Het

Other mutations in Or1e32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01520:Or1e32	APN	11	73,705,612 (GRCm39)	missense	probably damaging	0.97
IGL02976:Or1e32	APN	11	73,705,143 (GRCm39)	missense	probably damaging	0.98
IGL03344:Or1e32	APN	11	73,705,003 (GRCm39)	missense	probably benign	0.21
BB007:Or1e32	UTSW	11	73,705,926 (GRCm39)	start gained	probably benign
BB017:Or1e32	UTSW	11	73,705,926 (GRCm39)	start gained	probably benign
R0196:Or1e32	UTSW	11	73,705,731 (GRCm39)	missense	probably damaging	0.99
R0347:Or1e32	UTSW	11	73,705,137 (GRCm39)	missense	probably damaging	0.98
R0594:Or1e32	UTSW	11	73,705,443 (GRCm39)	missense	probably benign	0.12
R0940:Or1e32	UTSW	11	73,705,050 (GRCm39)	missense	probably damaging	1.00
R1233:Or1e32	UTSW	11	73,705,176 (GRCm39)	missense	probably damaging	1.00
R1300:Or1e32	UTSW	11	73,705,072 (GRCm39)	missense	probably benign	0.13
R1490:Or1e32	UTSW	11	73,705,197 (GRCm39)	missense	possibly damaging	0.95
R3111:Or1e32	UTSW	11	73,705,012 (GRCm39)	missense	probably benign
R4827:Or1e32	UTSW	11	73,705,547 (GRCm39)	nonsense	probably null
R4852:Or1e32	UTSW	11	73,705,074 (GRCm39)	missense	probably benign	0.11
R4992:Or1e32	UTSW	11	73,705,146 (GRCm39)	missense	probably damaging	1.00
R6193:Or1e32	UTSW	11	73,705,650 (GRCm39)	missense	probably benign
R7201:Or1e32	UTSW	11	73,705,167 (GRCm39)	missense	probably benign	0.19
R7310:Or1e32	UTSW	11	73,705,112 (GRCm39)	missense	probably damaging	0.98
R7930:Or1e32	UTSW	11	73,705,926 (GRCm39)	start gained	probably benign
R8471:Or1e32	UTSW	11	73,705,309 (GRCm39)	missense	probably benign	0.00
R8722:Or1e32	UTSW	11	73,705,882 (GRCm39)	missense	probably benign	0.03
R9415:Or1e32	UTSW	11	73,705,143 (GRCm39)	missense	probably damaging	0.98
X0021:Or1e32	UTSW	11	73,705,131 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTAAGGCTGTAGATGAAGGG -3'
(R):5'- CCTACTCACGGCTAGATTGTC -3'

Sequencing Primer
(F):5'- GTTGAGCATGGGAGTCACC -3'
(R):5'- ACTCACGGCTAGATTGTCTTTCTGTG -3'

Posted On

2021-07-15